Restriction map for
pFN21AE1062
(Linear) (Six-base) MAPSORT of: KIEE1062.seq Check: 6316 from: 1 to: 4362
KIEE1062.orf
With 182 enzymes: SgfI *
January 15, 2009 16:32 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 892 1452 2037 4362
Size: 892 560 585 2325
Fragments arranged by size:
2325 892 585 560
AccI GT'mk_AC
Cuts at: 0 3652 4362
Size: 3652 710
AclI AA'CG_TT
Cuts at: 0 145 4362
Size: 145 4217
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 656 800 1051 1361 2297 3164 3980 4259
Size: 656 144 251 310 936 867 816 279
Cuts at: 4259 4362
Size: 103
Fragments arranged by size:
936 867 816 656 310 279 251 144
103
AfeI AGC'GCT
Cuts at: 0 2434 4362
Size: 2434 1928
AflIII A'CryG_T
Cuts at: 0 1515 4362
Size: 1515 2847
AhdI GACnn_n'nnGTC
Cuts at: 0 2229 2813 3648 4362
Size: 2229 584 835 714
Fragments arranged by size:
2229 835 714 584
AleI CACnn'nnGTG
Cuts at: 0 3328 4362
Size: 3328 1034
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 358 2045 4362
Size: 358 1687 2317
Fragments arranged by size:
2317 1687 358
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 135 167 4362
Size: 135 32 4195
Fragments arranged by size:
4195 135 32
AlwNI CAG_nnn'CTG
Cuts at: 0 727 1750 1977 3181 4362
Size: 727 1023 227 1204 1181
Fragments arranged by size:
1204 1181 1023 727 227
ApoI r'AATT_y
Cuts at: 0 1024 1763 1995 2791 3438 3615 4362
Size: 1024 739 232 796 647 177 747
Fragments arranged by size:
1024 796 747 739 647 232 177
AseI AT'TA_AT
Cuts at: 0 1847 4362
Size: 1847 2515
AvaI C'yCGr_G
Cuts at: 0 70 1385 3819 4362
Size: 70 1315 2434 543
Fragments arranged by size:
2434 1315 543 70
AvrII C'CTAG_G
Cuts at: 0 3210 4362
Size: 3210 1152
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 0 3058 3091 4362
Size: 3058 33 1271
Fragments arranged by size:
3058 1271 33
BamHI G'GATC_C
Cuts at: 0 2457 3214 3470 4362
Size: 2457 757 256 892
Fragments arranged by size:
2457 892 757 256
BanI G'GyrC_C
Cuts at: 0 74 81 88 4362
Size: 74 7 7 4274
Fragments arranged by size:
4274 74 7 7
BanII G_rGCy'C
Cuts at: 0 21 1269 3265 4362
Size: 21 1248 1996 1097
Fragments arranged by size:
1996 1248 1097 21
BbeI G_GCGC'C
Cuts at: 0 78 85 92 4362
Size: 78 7 7 4270
Fragments arranged by size:
4270 78 7 7
BbsI GAAGACnn'nnnn_
Cuts at: 0 442 4012 4177 4274 4362
Size: 442 3570 165 97 88
Fragments arranged by size:
3570 442 165 97 88
BbvCI CC'TCA_GC
Cuts at: 0 31 4362
Size: 31 4331
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 2624 2658 3383 3417 4362
Size: 2624 34 725 34 945
Fragments arranged by size:
2624 945 725 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 0 174 4362
Size: 174 4188
BclI T'GATC_A
Cuts at: 0 3945 4362
Size: 3945 417
BglII A'GATC_T
Cuts at: 0 3462 4362
Size: 3462 900
Bme1580I G_kGCm'C
Cuts at: 0 3735 4236 4362
Size: 3735 501 126
Fragments arranged by size:
3735 501 126
BmgBI CAC'GTC
Cuts at: 0 1433 4362
Size: 1433 2929
BmrI ACTGGGnnnn_n'
Cuts at: 0 1952 3033 4223 4362
Size: 1952 1081 1190 139
Fragments arranged by size:
1952 1190 1081 139
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 1652 2351 4223 4362
Size: 1652 699 1872 139
Fragments arranged by size:
1872 1652 699 139
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 934 1283 1931 2018 2324 3203 3443 3497
Size: 934 349 648 87 306 879 240 54
Cuts at: 3497 3508 3762 3779 4070 4362
Size: 11 254 17 291 292
Fragments arranged by size:
934 879 648 349 306 292 291 254
240 87 54 17 11
Bpu10I CC'TnA_GC
Cuts at: 0 31 1620 4362
Size: 31 1589 2742
Fragments arranged by size:
2742 1589 31
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 977 3545 4362
Size: 977 2568 817
Fragments arranged by size:
2568 977 817
BsaI GGTCTCn'nnnn_
Cuts at: 0 1413 2104 3672 4243 4362
Size: 1413 691 1568 571 119
Fragments arranged by size:
1568 1413 691 571 119
BsaAI yAC'GTr
Cuts at: 0 1733 2298 2630 4362
Size: 1733 565 332 1732
Fragments arranged by size:
1733 1732 565 332
BsaHI Gr'CG_yC
Cuts at: 0 75 82 89 300 3317 4362
Size: 75 7 7 211 3017 1045
Fragments arranged by size:
3017 1045 211 75 7 7
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 135 165 1296 1326 3525 3555 4362
Size: 135 30 1131 30 2199 30 807
Fragments arranged by size:
2199 1131 807 135 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 19 871 1373 1958 2327 3263 3966 4362
Size: 19 852 502 585 369 936 703 396
Fragments arranged by size:
936 852 703 585 502 396 369 19
BseYI C'CCAG_C
Cuts at: 0 493 943 1680 2461 2709 3707 3716 3898
Size: 493 450 737 781 248 998 9 182
Cuts at: 3898 4136 4362
Size: 238 226
Fragments arranged by size:
998 781 737 493 450 248 238 226
182 9
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 3924 4264 4362
Size: 3924 340 98
Fragments arranged by size:
3924 340 98
BsiEI CG_ry'CG
Cuts at: 0 98 1580 4362
Size: 98 1482 2782
Fragments arranged by size:
2782 1482 98
BsiWI C'GTAC_G
Cuts at: 0 2298 4362
Size: 2298 2064
BsmI GAATG_Cn'
Cuts at: 0 137 345 617 2736 3012 4362
Size: 137 208 272 2119 276 1350
Fragments arranged by size:
2119 1350 276 272 208 137
BsmBI CGTCTCn'nnnn_
Cuts at: 0 3932 4362
Size: 3932 430
Bsp1286I G_dGCh'C
Cuts at: 0 21 1269 3265 3735 4236 4362
Size: 21 1248 1996 470 501 126
Fragments arranged by size:
1996 1248 501 470 126 21
BspHI T'CATG_A
Cuts at: 0 2610 3477 3530 4362
Size: 2610 867 53 832
Fragments arranged by size:
2610 867 832 53
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 436 892 1452 2037 4362
Size: 436 456 560 585 2325
Fragments arranged by size:
2325 585 560 456 436
BsrBI CCG'CTC
Cuts at: 0 12 107 2307 3239 4362
Size: 12 95 2200 932 1123
Fragments arranged by size:
2200 1123 932 95 12
BsrDI GCAATG_nn'
Cuts at: 0 342 633 1229 1761 4111 4362
Size: 342 291 596 532 2350 251
Fragments arranged by size:
2350 596 532 342 291 251
BsrFI r'CCGG_y
Cuts at: 0 174 3197 4202 4362
Size: 174 3023 1005 160
Fragments arranged by size:
3023 1005 174 160
BsrGI T'GTAC_A
Cuts at: 0 1583 4362
Size: 1583 2779
BssSI C'ACGA_G
Cuts at: 0 2267 4362
Size: 2267 2095
BstBI TT'CG_AA
Cuts at: 0 141 1938 3888 4362
Size: 141 1797 1950 474
Fragments arranged by size:
1950 1797 474 141
BstEII G'GTnAC_C
Cuts at: 0 2805 4362
Size: 2805 1557
BstXI CCAn_nnnn'nTGG
Cuts at: 0 2418 3333 3467 4311 4362
Size: 2418 915 134 844 51
Fragments arranged by size:
2418 915 844 134 51
BstYI r'GATC_y
Cuts at: 0 319 723 1452 2457 2559 3214 3462 3470
Size: 319 404 729 1005 102 655 248 8
Cuts at: 3470 4362
Size: 892
Fragments arranged by size:
1005 892 729 655 404 319 248 102
8
Bsu36I CC'TnA_GG
Cuts at: 0 728 1661 3174 4362
Size: 728 933 1513 1188
Fragments arranged by size:
1513 1188 933 728
BtgI C'CryG_G
Cuts at: 0 92 2847 3587 3829 4362
Size: 92 2755 740 242 533
Fragments arranged by size:
2755 740 533 242 92
BtsI GCAGTG_nn'
Cuts at: 0 1369 1639 4025 4362
Size: 1369 270 2386 337
Fragments arranged by size:
2386 1369 337 270
DraI TTT'AAA
Cuts at: 0 278 2771 4362
Size: 278 2493 1591
Fragments arranged by size:
2493 1591 278
DraIII CAC_nnn'GTG
Cuts at: 0 2475 2553 4167 4362
Size: 2475 78 1614 195
Fragments arranged by size:
2475 1614 195 78
DrdI GACnn_nn'nnGTC
Cuts at: 0 2133 4362
Size: 2133 2229
EaeI y'GGCC_r
Cuts at: 0 95 389 3986 4362
Size: 95 294 3597 376
Fragments arranged by size:
3597 376 294 95
EagI C'GGCC_G
Cuts at: 0 95 4362
Size: 95 4267
EarI CTCTTCn'nnn_
Cuts at: 0 207 2297 3927 4051 4362
Size: 207 2090 1630 124 311
Fragments arranged by size:
2090 1630 311 207 124
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 22 1452 4362
Size: 22 1430 2910
Fragments arranged by size:
2910 1430 22
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 656 800 934 1051 1283 1361 1931 2018
Size: 656 144 134 117 232 78 570 87
Cuts at: 2018 2297 2324 3164 3203 3443 3497 3508 3762
Size: 279 27 840 39 240 54 11 254
Cuts at: 3762 3779 3980 4070 4259 4362
Size: 17 201 90 189 103
Fragments arranged by size:
840 656 570 279 254 240 232 201
189 144 134 117 103 90 87 78
54 39 27 17 11
EcoNI CCTnn'n_nnAGG
Cuts at: 0 368 4362
Size: 368 3994
EcoO109I rG'GnC_Cy
Cuts at: 0 3133 3954 4090 4338 4362
Size: 3133 821 136 248 24
Fragments arranged by size:
3133 821 248 136 24
EcoRI G'AATT_C
Cuts at: 0 1024 2791 3438 4362
Size: 1024 1767 647 924
Fragments arranged by size:
1767 1024 924 647
EcoRV GAT'ATC
Cuts at: 0 737 3567 4362
Size: 737 2830 795
Fragments arranged by size:
2830 795 737
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 1018 1853 4362
Size: 1018 835 2509
Fragments arranged by size:
2509 1018 835
HaeII r_GCGC'y
Cuts at: 0 78 85 92 2436 4362
Size: 78 7 7 2344 1926
Fragments arranged by size:
2344 1926 78 7 7
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 172 204 721 753 2136 2168 2215 2247
Size: 172 32 517 32 1383 32 47 32
Cuts at: 2247 2449 2481 2529 2561 2799 2831 3199 3231
Size: 202 32 48 32 238 32 368 32
Cuts at: 3231 3525 3557 3634 3666 4362
Size: 294 32 77 32 696
Fragments arranged by size:
1383 696 517 368 294 238 202 172
77 48 47 32 32 32 32 32
32 32 32 32 32
HincII GTy'rAC
Cuts at: 0 3077 3579 4362
Size: 3077 502 783
Fragments arranged by size:
3077 783 502
HpaI GTT'AAC
Cuts at: 0 3077 4362
Size: 3077 1285
KasI G'GCGC_C
Cuts at: 0 74 81 88 4362
Size: 74 7 7 4274
Fragments arranged by size:
4274 74 7 7
MfeI C'AATT_G
Cuts at: 0 983 4362
Size: 983 3379
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 856 1132 1227 2896 3242 4244 4362
Size: 856 276 95 1669 346 1002 118
Fragments arranged by size:
1669 1002 856 346 276 118 95
MscI TGG'CCA
Cuts at: 0 391 3988 4362
Size: 391 3597 374
Fragments arranged by size:
3597 391 374
MslI CAynn'nnrTG
Cuts at: 0 2323 2416 2569 2932 3328 3331 3847 4258
Size: 2323 93 153 363 396 3 516 411
Cuts at: 4258 4362
Size: 104
Fragments arranged by size:
2323 516 411 396 363 153 104 93
3
MspA1I CmG'CkG
Cuts at: 0 94 2133 2142 4362
Size: 94 2039 9 2220
Fragments arranged by size:
2220 2039 94 9
NaeI GCC'GGC
Cuts at: 0 3199 4204 4362
Size: 3199 1005 158
Fragments arranged by size:
3199 1005 158
NarI GG'CG_CC
Cuts at: 0 75 82 89 4362
Size: 75 7 7 4273
Fragments arranged by size:
4273 75 7 7
NcoI C'CATG_G
Cuts at: 0 2847 3587 3829 4362
Size: 2847 740 242 533
Fragments arranged by size:
2847 740 533 242
NdeI CA'TA_TG
Cuts at: 0 1701 4362
Size: 1701 2661
NgoMIV G'CCGG_C
Cuts at: 0 3197 4202 4362
Size: 3197 1005 160
Fragments arranged by size:
3197 1005 160
NotI GC'GGCC_GC
Cuts at: 0 95 4362
Size: 95 4267
NspI r_CATG'y
Cuts at: 0 3651 3938 4362
Size: 3651 287 424
Fragments arranged by size:
3651 424 287
PflMI CCAn_nnn'nTGG
Cuts at: 0 842 1750 4362
Size: 842 908 2612
Fragments arranged by size:
2612 908 842
PfoI T'CCnGG_A
Cuts at: 0 311 1908 4086 4362
Size: 311 1597 2178 276
Fragments arranged by size:
2178 1597 311 276
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 135 167 4362
Size: 135 32 4195
Fragments arranged by size:
4195 135 32
PpuMI rG'GwC_Cy
Cuts at: 0 3133 3954 4090 4338 4362
Size: 3133 821 136 248 24
Fragments arranged by size:
3133 821 248 136 24
PshAI GACnn'nnGTC
Cuts at: 0 3138 4362
Size: 3138 1224
PsiI TTA'TAA
Cuts at: 0 1775 4362
Size: 1775 2587
PvuII CAG'CTG
Cuts at: 0 2133 2142 4362
Size: 2133 9 2220
Fragments arranged by size:
2220 2133 9
SacII CC_GC'GG
Cuts at: 0 95 4362
Size: 95 4267
SanDI GG'GwC_CC
Cuts at: 0 3133 4362
Size: 3133 1229
SapI GCTCTTCn'nnn_
Cuts at: 0 207 4051 4362
Size: 207 3844 311
Fragments arranged by size:
3844 311 207
SfoI GGC'GCC
Cuts at: 0 76 83 90 4362
Size: 76 7 7 4272
Fragments arranged by size:
4272 76 7 7
SmaI CCC'GGG
Cuts at: 0 3821 4362
Size: 3821 541
SmlI C'TyrA_G
Cuts at: 0 992 3560 4362
Size: 992 2568 802
Fragments arranged by size:
2568 992 802
StuI AGG'CCT
Cuts at: 0 2086 2544 4362
Size: 2086 458 1818
Fragments arranged by size:
2086 1818 458
StyI C'CwwG_G
Cuts at: 0 1493 1982 2114 2847 2885 3210 3587 3829
Size: 1493 489 132 733 38 325 377 242
Cuts at: 3829 4362
Size: 533
Fragments arranged by size:
1493 733 533 489 377 325 242 132
38
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 1550 2494 2898 4064 4362
Size: 1550 944 404 1166 298
Fragments arranged by size:
1550 1166 944 404 298
TatI w'GTAC_w
Cuts at: 0 1583 1781 2723 2827 2995 3428 4362
Size: 1583 198 942 104 168 433 934
Fragments arranged by size:
1583 942 934 433 198 168 104
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 4089 4121 4362
Size: 4089 32 241
Fragments arranged by size:
4089 241 32
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 251 548 839 1115 2451 3273 4260 4362
Size: 251 297 291 276 1336 822 987 102
Fragments arranged by size:
1336 987 822 297 291 276 251 102
XmaI C'CCGG_G
Cuts at: 0 3819 4362
Size: 3819 543
XmnI GAAnn'nnTTC
Cuts at: 0 2867 3884 4060 4362
Size: 2867 1017 176 302
Fragments arranged by size:
2867 1017 302 176
Enzymes that do cut:
AarI AccI AclI AcuI AfeI AflIII AhdI AleI
AlfI AloI AlwNI ApoI AseI AvaI AvrII BaeI
BamHI BanI BanII BbeI BbsI BbvCI BcgI BciVI
BclI BglII Bme1580I BmgBI BmrI BplI BpmI Bpu10I
BpuEI BsaI BsaAI BsaHI BsaXI BseRI BseYI BsgI
BsiEI BsiWI BsmI BsmBI Bsp1286I BspHI BspMI BsrBI
BsrDI BsrFI BsrGI BssSI BstBI BstEII BstXI BstYI
Bsu36I BtgI BtsI DraI DraIII DrdI EaeI EagI
EarI EciI Eco57MI EcoNI EcoO109I EcoRI EcoRV FalI
HaeII Hin4I HincII HpaI KasI MfeI MmeI MscI
MslI MspA1I NaeI NarI NcoI NdeI NgoMIV NotI
NspI PflMI PfoI PpiI PpuMI PshAI PsiI PvuII
SacII SanDI SapI SfoI SmaI SmlI StuI StyI
TaqII TatI TstI XcmI XmaI XmnI
Enzymes that do not cut:
AatII Acc65I AflII AgeI ApaI ApaLI AscI AsiSI
BfrBI BglI BlpI BmtI BsaBI BsaWI BsiHKAI BspEI
BssHII BstAPI BstZ17I BtgZI ClaI CspCI EcoICRI FseI
FspI FspAI HindIII KpnI MluI NheI NruI NsiI
PacI PasI PciI PmeI PmlI PspOMI PspXI PsrI
PstI PvuI RsrII SacI SalI SbfI ScaI SexAI
SfcI SfiI SgfI SgrAI SnaBI SpeI SphI SrfI
SspI SwaI Tth111I XbaI XhoI ZraI