(Linear) (Six-base) MAPSORT of: KIEE4478.seq Check: 4809 from: 1 to: 1488 KIEE4478.orf With 182 enzymes: SgfI * March 22, 2012 18:09 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 0 886 1488 Size: 886 602 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 0 560 1161 1488 Size: 560 601 327 Fragments arranged by size: 601 560 327 AhdI GACnn_n'nnGTC Cuts at: 0 250 1488 Size: 250 1238 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 0 295 1488 Size: 295 1193 ApaI G_GGCC'C Cuts at: 0 1189 1488 Size: 1189 299 ApoI r'AATT_y Cuts at: 0 118 494 523 1488 Size: 118 376 29 965 Fragments arranged by size: 965 376 118 29 BanI G'GyrC_C Cuts at: 0 454 691 1488 Size: 454 237 797 Fragments arranged by size: 797 454 237 BanII G_rGCy'C Cuts at: 0 779 911 1189 1488 Size: 779 132 278 299 Fragments arranged by size: 779 299 278 132 BciVI GTATCCnnnnn_n' Cuts at: 0 714 1488 Size: 714 774 BglII A'GATC_T Cuts at: 0 431 509 1420 1488 Size: 431 78 911 68 Fragments arranged by size: 911 431 78 68 BlpI GC'TnA_GC Cuts at: 0 665 1488 Size: 665 823 Bme1580I G_kGCm'C Cuts at: 0 416 694 1189 1488 Size: 416 278 495 299 Fragments arranged by size: 495 416 299 278 BmgBI CAC'GTC Cuts at: 0 179 1488 Size: 179 1309 BmrI ACTGGGnnnn_n' Cuts at: 0 92 571 1488 Size: 92 479 917 Fragments arranged by size: 917 479 92 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 661 811 1488 Size: 661 150 677 Fragments arranged by size: 677 661 150 Bpu10I CC'TnA_GC Cuts at: 0 1466 1488 Size: 1466 22 BsaI GGTCTCn'nnnn_ Cuts at: 0 1348 1465 1488 Size: 1348 117 23 Fragments arranged by size: 1348 117 23 BsaHI Gr'CG_yC Cuts at: 0 1436 1488 Size: 1436 52 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 0 1216 1246 1274 1304 1488 Size: 1216 30 28 30 184 Fragments arranged by size: 1216 184 30 30 28 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 0 670 1488 Size: 670 818 BseYI C'CCAG_C Cuts at: 0 21 477 1194 1488 Size: 21 456 717 294 Fragments arranged by size: 717 456 294 21 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 0 384 617 1433 1488 Size: 384 233 816 55 Fragments arranged by size: 816 384 233 55 BsiHKAI G_wGCw'C Cuts at: 0 779 827 1488 Size: 779 48 661 Fragments arranged by size: 779 661 48 BsmI GAATG_Cn' Cuts at: 0 666 1488 Size: 666 822 BsmBI CGTCTCn'nnnn_ Cuts at: 0 185 1488 Size: 185 1303 Bsp1286I G_dGCh'C Cuts at: 0 416 694 779 827 911 1189 1488 Size: 416 278 85 48 84 278 299 Fragments arranged by size: 416 299 278 278 85 84 48 BspHI T'CATG_A Cuts at: 0 1043 1488 Size: 1043 445 BspMI ACCTGCnnnn'nnnn_ Cuts at: 0 886 1488 Size: 886 602 BsrFI r'CCGG_y Cuts at: 0 1213 1306 1488 Size: 1213 93 182 Fragments arranged by size: 1213 182 93 BsrGI T'GTAC_A Cuts at: 0 419 1488 Size: 419 1069 BstAPI GCAn_nnn'nTGC Cuts at: 0 1411 1488 Size: 1411 77 BstEII G'GTnAC_C Cuts at: 0 15 1488 Size: 15 1473 BstXI CCAn_nnnn'nTGG Cuts at: 0 514 1488 Size: 514 974 BstYI r'GATC_y Cuts at: 0 431 509 671 1420 1488 Size: 431 78 162 749 68 Fragments arranged by size: 749 431 162 78 68 BtgI C'CryG_G Cuts at: 0 1359 1488 Size: 1359 129 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 0 1216 1488 Size: 1216 272 BtsI GCAGTG_nn' Cuts at: 0 1214 1488 Size: 1214 274 DraIII CAC_nnn'GTG Cuts at: 0 1411 1488 Size: 1411 77 EaeI y'GGCC_r Cuts at: 0 200 550 1166 1211 1488 Size: 200 350 616 45 277 Fragments arranged by size: 616 350 277 200 45 EarI CTCTTCn'nnn_ Cuts at: 0 253 391 536 647 1180 1261 1488 Size: 253 138 145 111 533 81 227 Fragments arranged by size: 533 253 227 145 138 111 81 EcoICRI GAG'CTC Cuts at: 0 777 1488 Size: 777 711 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 0 560 661 811 1161 1488 Size: 560 101 150 350 327 Fragments arranged by size: 560 350 327 150 101 EcoO109I rG'GnC_Cy Cuts at: 0 1144 1185 1186 1488 Size: 1144 41 1 302 Fragments arranged by size: 1144 302 41 1 EcoRI G'AATT_C Cuts at: 0 494 523 1488 Size: 494 29 965 Fragments arranged by size: 965 494 29 EcoRV GAT'ATC Cuts at: 0 1380 1488 Size: 1380 108 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 0 236 268 351 383 501 533 620 652 Size: 236 32 83 32 118 32 87 32 Cuts at: 652 791 823 1488 Size: 139 32 665 Fragments arranged by size: 665 236 139 118 87 83 32 32 32 32 32 HincII GTy'rAC Cuts at: 0 255 291 1488 Size: 255 36 1197 Fragments arranged by size: 1197 255 36 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 0 222 471 1227 1247 1259 1488 Size: 222 249 756 20 12 229 Fragments arranged by size: 756 249 229 222 20 12 MscI TGG'CCA Cuts at: 0 202 1488 Size: 202 1286 MslI CAynn'nnrTG Cuts at: 0 1042 1444 1488 Size: 1042 402 44 Fragments arranged by size: 1042 402 44 MspA1I CmG'CkG Cuts at: 0 477 555 681 1198 1267 1488 Size: 477 78 126 517 69 221 Fragments arranged by size: 517 477 221 126 78 69 NaeI GCC'GGC Cuts at: 0 1215 1488 Size: 1215 273 NdeI CA'TA_TG Cuts at: 0 1004 1488 Size: 1004 484 NgoMIV G'CCGG_C Cuts at: 0 1213 1488 Size: 1213 275 PflMI CCAn_nnn'nTGG Cuts at: 0 1154 1488 Size: 1154 334 PfoI T'CCnGG_A Cuts at: 0 716 1488 Size: 716 772 PspOMI G'GGCC_C Cuts at: 0 1185 1488 Size: 1185 303 PvuII CAG'CTG Cuts at: 0 477 1198 1488 Size: 477 721 290 Fragments arranged by size: 721 477 290 SacI G_AGCT'C Cuts at: 0 779 1488 Size: 779 709 SapI GCTCTTCn'nnn_ Cuts at: 0 536 1261 1488 Size: 536 725 227 Fragments arranged by size: 725 536 227 SfcI C'TryA_G Cuts at: 0 956 1488 Size: 956 532 StuI AGG'CCT Cuts at: 0 1013 1488 Size: 1013 475 StyI C'CwwG_G Cuts at: 0 531 1014 1488 Size: 531 483 474 Fragments arranged by size: 531 483 474 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 0 709 1488 Size: 709 779 TatI w'GTAC_w Cuts at: 0 419 1488 Size: 419 1069 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 0 485 517 1488 Size: 485 32 971 Fragments arranged by size: 971 485 32 Tth111I GACn'n_nGTC Cuts at: 0 1435 1488 Size: 1435 53 Enzymes that do cut: AarI AcuI AhdI AlfI ApaI ApoI BanI BanII BciVI BglII BlpI Bme1580I BmgBI BmrI BpmI Bpu10I BsaI BsaHI BsaXI BseRI BseYI BsgI BsiHKAI BsmI BsmBI Bsp1286I BspHI BspMI BsrFI BsrGI BstAPI BstEII BstXI BstYI BtgI BtgZI BtsI DraIII EaeI EarI EcoICRI Eco57MI EcoO109I EcoRI EcoRV Hin4I HincII MmeI MscI MslI MspA1I NaeI NdeI NgoMIV PflMI PfoI PspOMI PvuII SacI SapI SfcI StuI StyI TaqII TatI TstI Tth111I Enzymes that do not cut: AatII AccI Acc65I AclI AfeI AflII AflIII AgeI AleI AloI AlwNI ApaLI AscI AseI AsiSI AvaI AvrII BaeI BamHI BbeI BbsI BbvCI BcgI BclI BfrBI BglI BmtI BplI BpuEI BsaAI BsaBI BsaWI BsiEI BsiWI BspEI BsrBI BsrDI BssHII BssSI BstBI BstZ17I Bsu36I ClaI CspCI DraI DrdI EagI EciI EcoNI FalI FseI FspI FspAI HaeII HindIII HpaI KasI KpnI MfeI MluI NarI NcoI NheI NotI NruI NsiI NspI PacI PasI PciI PmeI PmlI PpiI PpuMI PshAI PsiI PspXI PsrI PstI PvuI RsrII SacII SalI SanDI SbfI ScaI SexAI SfiI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SrfI SspI SwaI XbaI XcmI XhoI XmaI XmnI ZraI