(Circular) (Six-base) MAPSORT of: pFN21AE4453.seq Check: 9098 from: 1 to: 6124 pFN21AE4453 6124 bp With 182 enzymes: SgfI * March 28, 2012 13:53 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 2021 2021 Size: 6124 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 2664 3934 278 Size: 53 83 186 936 1128 1270 2468 Fragments arranged by size: 2468 1270 1128 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3467 1937 Size: 1530 4594 Acc65I G'GTAC_C Cuts at: 2460 3445 2460 Size: 985 5139 AclI AA'CG_TT Cuts at: 1563 4251 4624 1563 Size: 2688 373 3063 Fragments arranged by size: 3063 2688 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2203 2593 2756 3388 4187 5571 5839 1632 Size: 571 390 163 632 799 1384 268 1917 Fragments arranged by size: 1917 1384 799 632 571 390 268 163 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5927 AflIII A'CryG_T Cuts at: 1203 5023 6000 1203 Size: 3820 977 1327 Fragments arranged by size: 3820 1327 977 AgeI A'CCGG_T Cuts at: 4955 4955 Size: 6124 AhdI GACnn_n'nnGTC Cuts at: 4853 4853 Size: 6124 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 6124 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 3261 1184 Size: 2077 4047 AlwNI CAG_nnn'CTG Cuts at: 3031 3136 3867 5439 3031 Size: 105 731 1572 3716 Fragments arranged by size: 3716 1572 731 105 ApaLI G'TGCA_C Cuts at: 4181 5337 4181 Size: 1156 4968 ApoI r'AATT_y Cuts at: 1493 3433 3659 3811 5920 1493 Size: 1940 226 152 2109 1697 Fragments arranged by size: 2109 1940 1697 226 152 AseI AT'TA_AT Cuts at: 160 1032 4678 160 Size: 872 3646 1606 Fragments arranged by size: 3646 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6124 AvaI C'yCGr_G Cuts at: 1870 1943 3449 1870 Size: 73 1506 4545 Fragments arranged by size: 4545 1506 73 BamHI G'GATC_C Cuts at: 3454 3821 3454 Size: 367 5757 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2460 3445 4900 618 Size: 325 217 627 673 985 1455 1842 Fragments arranged by size: 1842 1455 985 673 627 325 217 BanII G_rGCy'C Cuts at: 1391 2054 3012 3395 3422 3443 1391 Size: 663 958 383 27 21 4072 Fragments arranged by size: 4072 958 663 383 27 21 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 3230 3325 928 Size: 971 1331 95 3727 Fragments arranged by size: 3727 1331 971 95 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 4315 4349 1903 Size: 34 2378 34 3678 Fragments arranged by size: 3678 2378 34 34 BciVI GTATCCnnnnn_n' Cuts at: 4016 5226 4016 Size: 1210 4914 BclI T'GATC_A Cuts at: 1548 1548 Size: 6124 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2594 4735 136 Size: 107 122 71 631 605 922 2141 1525 Fragments arranged by size: 2141 1525 922 631 605 122 107 71 BglII A'GATC_T Cuts at: 3207 6119 3207 Size: 2912 3212 BlpI GC'TnA_GC Cuts at: 3056 3541 3056 Size: 485 5639 Bme1580I G_kGCm'C Cuts at: 1790 2131 4185 5341 1790 Size: 341 2054 1156 2573 Fragments arranged by size: 2573 2054 1156 341 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 6124 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2472 3855 4813 Size: 416 53 83 399 24 1055 1383 958 Cuts at: 4813 442 Size: 1753 Fragments arranged by size: 1753 1383 1055 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6124 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3409 3409 Size: 6124 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2020 2071 2438 2735 3008 3053 3095 3107 Size: 781 51 367 297 273 45 42 12 Cuts at: 3107 3254 3287 4784 1239 Size: 147 33 1497 2579 Fragments arranged by size: 2579 1497 781 367 297 273 147 51 45 42 33 12 Bpu10I CC'TnA_GC Cuts at: 2849 2930 2849 Size: 81 6043 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 3365 4249 5114 5412 3365 Size: 884 865 298 4077 Fragments arranged by size: 4077 884 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2912 3086 4787 882 Size: 617 1413 174 1701 2219 Fragments arranged by size: 2219 1701 1413 617 174 BsaAI yAC'GTr Cuts at: 493 1204 3915 493 Size: 711 2711 2702 Fragments arranged by size: 2711 2702 711 BsaBI GATnn'nnATC Cuts at: 2723 6118 2723 Size: 3395 2729 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2661 3931 4313 4948 Size: 53 83 186 936 1128 1270 382 635 Cuts at: 4948 275 Size: 1451 Fragments arranged by size: 1451 1270 1128 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 3012 4557 4955 5229 5376 5787 3012 Size: 1545 398 274 147 411 3349 Fragments arranged by size: 3349 1545 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 3275 3305 1178 Size: 30 165 30 1872 30 3997 Fragments arranged by size: 3997 1872 165 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2023 2089 2152 2164 2435 2756 2786 2789 Size: 837 66 63 12 271 321 30 3 Cuts at: 2789 2957 3104 3167 3331 3334 1186 Size: 168 147 63 164 3 3976 Fragments arranged by size: 3976 837 321 271 168 164 147 66 63 63 30 12 3 3 BseYI C'CCAG_C Cuts at: 2132 2194 3051 3165 3294 5327 5823 2132 Size: 62 857 114 129 2033 496 2433 Fragments arranged by size: 2433 2033 857 496 129 114 62 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2817 1163 Size: 573 1081 4470 Fragments arranged by size: 4470 1081 573 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3574 4335 5363 1540 Size: 275 186 1573 761 1028 2301 Fragments arranged by size: 2301 1573 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2416 3012 3024 3395 3422 4185 4270 5341 2416 Size: 596 12 371 27 763 85 1071 3199 Fragments arranged by size: 3199 1071 763 596 371 85 27 12 BsmI GAATG_Cn' Cuts at: 2563 3635 3728 2563 Size: 1072 93 4959 Fragments arranged by size: 4959 1072 93 BsmBI CGTCTCn'nnnn_ Cuts at: 2904 2904 Size: 6124 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2054 2131 2416 3012 3024 3395 3422 Size: 399 264 77 285 596 12 371 27 Cuts at: 3422 3443 4185 4270 5341 1391 Size: 21 742 85 1071 2174 Fragments arranged by size: 2174 1071 742 596 399 371 285 264 85 77 27 21 12 BspEI T'CCGG_A Cuts at: 3012 5787 3012 Size: 2775 3349 BspHI T'CATG_A Cuts at: 4013 5743 4013 Size: 1730 4394 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2021 3479 844 Size: 1177 1458 3489 Fragments arranged by size: 3489 1458 1177 BsrBI CCG'CTC Cuts at: 1394 1823 4011 1394 Size: 429 2188 3507 Fragments arranged by size: 3507 2188 429 BsrDI GCAATG_nn' Cuts at: 65 1248 2538 4619 4793 65 Size: 1183 1290 2081 174 1396 Fragments arranged by size: 2081 1396 1290 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2801 4768 4955 1700 Size: 110 991 1967 187 2869 Fragments arranged by size: 2869 1967 991 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 6124 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6124 BssSI C'ACGA_G Cuts at: 2768 4184 5196 2768 Size: 1416 1012 3696 Fragments arranged by size: 3696 1416 1012 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 6124 BstBI TT'CG_AA Cuts at: 4928 4928 Size: 6124 BstEII G'GTnAC_C Cuts at: 1184 2359 1184 Size: 1175 4949 BstYI r'GATC_y Cuts at: 1972 2448 3126 3207 3454 3821 4206 4223 5675 Size: 476 678 81 247 367 385 17 1452 Cuts at: 5675 5761 5773 6119 1972 Size: 86 12 346 1977 Fragments arranged by size: 1977 1452 678 476 385 367 346 247 86 81 17 12 Bsu36I CC'TnA_GG Cuts at: 2525 2756 2525 Size: 231 5893 BtgI C'CryG_G Cuts at: 513 1065 513 Size: 552 5572 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5385 Fragments arranged by size: 5385 683 56 BtsI GCAGTG_nn' Cuts at: 2356 3001 3151 3640 4434 4454 2356 Size: 645 150 489 794 20 4026 Fragments arranged by size: 4026 794 645 489 150 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6124 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 6089 DraI TTT'AAA Cuts at: 3428 3428 Size: 6124 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 5131 809 Size: 507 3815 1802 Fragments arranged by size: 3815 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2109 2544 2700 Size: 54 1425 50 275 18 279 435 156 Cuts at: 2700 3307 3571 4460 6089 8 Size: 607 264 889 1629 43 Fragments arranged by size: 1629 1425 889 607 435 279 275 264 156 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3571 1537 Size: 275 1759 4090 Fragments arranged by size: 4090 1759 275 EarI CTCTTCn'nnn_ Cuts at: 1353 3358 4054 1353 Size: 2005 696 3423 Fragments arranged by size: 3423 2005 696 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2820 4711 5085 5231 387 Size: 1064 138 1231 1891 374 146 1280 Fragments arranged by size: 1891 1280 1231 1064 374 146 138 EcoICRI GAG'CTC Cuts at: 3010 3393 3420 3010 Size: 383 27 5714 Fragments arranged by size: 5714 383 27 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2020 2071 2203 2438 2593 2735 2756 Size: 393 388 51 132 235 155 142 21 Cuts at: 2756 3008 3053 3095 3107 3254 3287 3388 4187 Size: 252 45 42 12 147 33 101 799 Cuts at: 4187 4784 5571 5839 1239 Size: 597 787 268 1524 Fragments arranged by size: 1524 799 787 597 393 388 268 252 235 155 147 142 132 101 51 45 42 33 21 12 EcoNI CCTnn'n_nnAGG Cuts at: 2402 2738 2755 3266 2402 Size: 336 17 511 5260 Fragments arranged by size: 5260 511 336 17 EcoO109I rG'GnC_Cy Cuts at: 2118 2521 3028 2118 Size: 403 507 5214 Fragments arranged by size: 5214 507 403 EcoRI G'AATT_C Cuts at: 3433 3811 3433 Size: 378 5746 FspI TGC'GCA Cuts at: 2562 4630 2562 Size: 2068 4056 HaeII r_GCGC'y Cuts at: 1678 5271 1678 Size: 3593 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2058 2090 2770 2802 3032 Size: 32 135 32 486 32 680 32 230 Cuts at: 3032 3064 4765 4797 4839 4871 1373 Size: 32 1701 32 42 32 2626 Fragments arranged by size: 2626 1701 680 486 230 135 42 32 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3468 3714 669 Size: 988 281 1530 246 3079 Fragments arranged by size: 3079 1530 988 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 6124 HpaI GTT'AAC Cuts at: 3714 3714 Size: 6124 KpnI G_GTAC'C Cuts at: 2464 3449 2464 Size: 985 5139 MfeI C'AATT_G Cuts at: 3723 3723 Size: 6124 MluI A'CGCG_T Cuts at: 6000 6000 Size: 6124 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2664 2991 5238 5422 1305 Size: 1359 327 2247 184 2007 Fragments arranged by size: 2247 2007 1359 327 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2702 3309 10 Size: 54 1425 343 870 607 2825 Fragments arranged by size: 2825 1425 870 607 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 4082 4441 4600 518 Size: 648 2916 359 159 2042 Fragments arranged by size: 2916 2042 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2473 3118 3160 3540 4217 5365 5610 1606 Size: 867 645 42 380 677 1148 245 2120 Fragments arranged by size: 2120 1148 867 677 645 380 245 42 NcoI C'CATG_G Cuts at: 513 1065 513 Size: 552 5572 NdeI CA'TA_TG Cuts at: 387 387 Size: 6124 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6124 NotI GC'GGCC_GC Cuts at: 3571 3571 Size: 6124 NspI r_CATG'y Cuts at: 1132 1229 3115 3482 5027 1132 Size: 97 1886 367 1545 2229 Fragments arranged by size: 2229 1886 1545 367 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 6124 PciI A'CATG_T Cuts at: 5023 5023 Size: 6124 PflMI CCAn_nnn'nTGG Cuts at: 1108 3031 1108 Size: 1923 4201 PmeI GTTT'AAAC Cuts at: 3428 3428 Size: 6124 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2909 2941 4193 4225 5706 5738 2909 Size: 32 1252 32 1481 32 3295 Fragments arranged by size: 3295 1481 1252 32 32 32 PpuMI rG'GwC_Cy Cuts at: 2118 2521 2118 Size: 403 5721 PshAI GACnn'nnGTC Cuts at: 1613 2496 1613 Size: 883 5241 PsiI TTA'TAA Cuts at: 3694 3694 Size: 6124 PstI C_TGCA'G Cuts at: 830 2018 3048 3476 830 Size: 1188 1030 428 3478 Fragments arranged by size: 3478 1188 1030 428 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6124 PvuII CAG'CTG Cuts at: 3118 3160 3118 Size: 42 6082 SacI G_AGCT'C Cuts at: 3012 3395 3422 3012 Size: 383 27 5714 Fragments arranged by size: 5714 383 27 SalI G'TCGA_C Cuts at: 1936 3466 1936 Size: 1530 4594 SbfI CC_TGCA'GG Cuts at: 3476 3476 Size: 6124 ScaI AGT'ACT Cuts at: 4372 6084 4372 Size: 1712 4412 SexAI A'CCwGG_T Cuts at: 2921 2921 Size: 6124 SfcI C'TryA_G Cuts at: 826 1045 2014 2494 3044 3472 4607 5288 5479 Size: 219 969 480 550 428 1135 681 191 Cuts at: 5479 826 Size: 1471 Fragments arranged by size: 1471 1135 969 681 550 480 428 219 191 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6124 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6124 SmaI CCC'GGG Cuts at: 1872 3451 1872 Size: 1579 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 3380 4228 5129 5391 820 Size: 197 926 1437 848 901 262 1553 Fragments arranged by size: 1553 1437 926 901 848 262 197 SnaBI TAC'GTA Cuts at: 493 3915 493 Size: 3422 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 6124 SphI G_CATG'C Cuts at: 1132 3115 3482 1132 Size: 1983 367 3774 Fragments arranged by size: 3774 1983 367 SspI AAT'ATT Cuts at: 5 52 4048 5 Size: 47 3996 2081 Fragments arranged by size: 3996 2081 47 StuI AGG'CCT Cuts at: 1517 2733 1517 Size: 1216 4908 StyI C'CwwG_G Cuts at: 513 1065 2272 3563 513 Size: 552 1207 1291 3074 Fragments arranged by size: 3074 1291 1207 552 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2288 2954 4151 4168 4321 4506 4951 Size: 1255 708 666 1197 17 153 185 445 Cuts at: 4951 325 Size: 1498 Fragments arranged by size: 1498 1255 1197 708 666 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 4370 6082 96 Size: 274 80 33 51 1443 2393 1712 138 Fragments arranged by size: 2393 1712 1443 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 3275 3307 6084 6116 3275 Size: 32 2777 32 3283 Fragments arranged by size: 3283 2777 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 6124 XbaI T'CTAG_A Cuts at: 3460 3460 Size: 6124 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2434 2710 2974 3137 3302 2434 Size: 276 264 163 165 5256 Fragments arranged by size: 5256 276 264 165 163 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 6124 XmaI C'CCGG_G Cuts at: 1870 3449 1870 Size: 1579 4545 XmnI GAAnn'nnTTC Cuts at: 4253 4253 Size: 6124 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 2662 3932 276 Size: 53 83 186 936 1128 1270 2468 Fragments arranged by size: 2468 1270 1128 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PmeI PpiI PpuMI PshAI PsiI PstI PvuI PvuII SacI SalI SbfI ScaI SexAI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AfeI AloI ApaI AscI AvrII BaeI BbeI BbvCI BfrBI BsiWI BstXI BstZ17I DraIII EcoRV FalI FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PfoI PmlI PspOMI PspXI PsrI RsrII SacII SanDI SapI SfiI SfoI SrfI SwaI