(Linear) (Six-base) MAPSORT of: KIEE4453.seq Check: 233 from: 1 to: 1419 KIEE4453.orf With 182 enzymes: SgfI * March 22, 2012 18:04 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 0 16 1419 Size: 16 1403 AatII G_ACGT'C Cuts at: 0 659 1419 Size: 659 760 Acc65I G'GTAC_C Cuts at: 0 455 1419 Size: 455 964 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 0 198 588 751 1383 1419 Size: 198 390 163 632 36 Fragments arranged by size: 632 390 198 163 36 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 0 1256 1419 Size: 1256 163 AlwNI CAG_nnn'CTG Cuts at: 0 1026 1131 1419 Size: 1026 105 288 Fragments arranged by size: 1026 288 105 BanI G'GyrC_C Cuts at: 0 455 1419 Size: 455 964 BanII G_rGCy'C Cuts at: 0 49 1007 1390 1417 1419 Size: 49 958 383 27 2 Fragments arranged by size: 958 383 49 27 2 BbsI GAAGACnn'nnnn_ Cuts at: 0 1225 1320 1419 Size: 1225 95 99 Fragments arranged by size: 1225 99 95 BglI GCCn_nnn'nGGC Cuts at: 0 589 1419 Size: 589 830 BglII A'GATC_T Cuts at: 0 1202 1419 Size: 1202 217 BlpI GC'TnA_GC Cuts at: 0 1051 1419 Size: 1051 368 Bme1580I G_kGCm'C Cuts at: 0 126 1419 Size: 126 1293 BmrI ACTGGGnnnn_n' Cuts at: 0 467 1419 Size: 467 952 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 0 1404 1419 Size: 1404 15 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 15 66 433 730 1003 1048 1090 1102 Size: 15 51 367 297 273 45 42 12 Cuts at: 1102 1249 1282 1419 Size: 147 33 137 Fragments arranged by size: 367 297 273 147 137 51 45 42 33 15 12 Bpu10I CC'TnA_GC Cuts at: 0 844 925 1419 Size: 844 81 494 Fragments arranged by size: 844 494 81 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 1360 1419 Size: 1360 59 BsaI GGTCTCn'nnnn_ Cuts at: 0 907 1081 1419 Size: 907 174 338 Fragments arranged by size: 907 338 174 BsaBI GATnn'nnATC Cuts at: 0 718 1419 Size: 718 701 BsaHI Gr'CG_yC Cuts at: 0 656 1419 Size: 656 763 BsaWI w'CCGG_w Cuts at: 0 1007 1419 Size: 1007 412 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 0 1270 1300 1419 Size: 1270 30 119 Fragments arranged by size: 1270 119 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 0 18 84 147 159 430 751 781 784 Size: 18 66 63 12 271 321 30 3 Cuts at: 784 952 1099 1162 1326 1329 1419 Size: 168 147 63 164 3 90 Fragments arranged by size: 321 271 168 164 147 90 66 63 63 30 18 12 3 3 BseYI C'CCAG_C Cuts at: 0 127 189 1046 1160 1289 1419 Size: 127 62 857 114 129 130 Fragments arranged by size: 857 130 129 127 114 62 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 0 812 1419 Size: 812 607 BsiHKAI G_wGCw'C Cuts at: 0 411 1007 1019 1390 1417 1419 Size: 411 596 12 371 27 2 Fragments arranged by size: 596 411 371 27 12 2 BsmI GAATG_Cn' Cuts at: 0 558 1419 Size: 558 861 BsmBI CGTCTCn'nnnn_ Cuts at: 0 899 1419 Size: 899 520 Bsp1286I G_dGCh'C Cuts at: 0 49 126 411 1007 1019 1390 1417 1419 Size: 49 77 285 596 12 371 27 2 Fragments arranged by size: 596 371 285 77 49 27 12 2 BspEI T'CCGG_A Cuts at: 0 1007 1419 Size: 1007 412 BspMI ACCTGCnnnn'nnnn_ Cuts at: 0 16 1419 Size: 16 1403 BsrDI GCAATG_nn' Cuts at: 0 533 1419 Size: 533 886 BsrFI r'CCGG_y Cuts at: 0 796 1419 Size: 796 623 BssSI C'ACGA_G Cuts at: 0 763 1419 Size: 763 656 BstEII G'GTnAC_C Cuts at: 0 354 1419 Size: 354 1065 BstYI r'GATC_y Cuts at: 0 443 1121 1202 1419 Size: 443 678 81 217 Fragments arranged by size: 678 443 217 81 Bsu36I CC'TnA_GG Cuts at: 0 520 751 1419 Size: 520 231 668 Fragments arranged by size: 668 520 231 BtsI GCAGTG_nn' Cuts at: 0 351 996 1146 1419 Size: 351 645 150 273 Fragments arranged by size: 645 351 273 150 EaeI y'GGCC_r Cuts at: 0 104 539 695 1302 1419 Size: 104 435 156 607 117 Fragments arranged by size: 607 435 156 117 104 EarI CTCTTCn'nnn_ Cuts at: 0 1353 1419 Size: 1353 66 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 0 815 1419 Size: 815 604 EcoICRI GAG'CTC Cuts at: 0 1005 1388 1415 1419 Size: 1005 383 27 4 Fragments arranged by size: 1005 383 27 4 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 0 15 66 198 433 588 730 751 1003 Size: 15 51 132 235 155 142 21 252 Cuts at: 1003 1048 1090 1102 1249 1282 1383 1419 Size: 45 42 12 147 33 101 36 Fragments arranged by size: 252 235 155 147 142 132 101 51 45 42 36 33 21 15 12 EcoNI CCTnn'n_nnAGG Cuts at: 0 397 733 750 1261 1419 Size: 397 336 17 511 158 Fragments arranged by size: 511 397 336 158 17 EcoO109I rG'GnC_Cy Cuts at: 0 113 516 1023 1419 Size: 113 403 507 396 Fragments arranged by size: 507 403 396 113 FspI TGC'GCA Cuts at: 0 557 1419 Size: 557 862 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 0 53 85 765 797 1027 1059 1419 Size: 53 32 680 32 230 32 360 Fragments arranged by size: 680 360 230 53 32 32 32 KpnI G_GTAC'C Cuts at: 0 459 1419 Size: 459 960 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 0 659 986 1419 Size: 659 327 433 Fragments arranged by size: 659 433 327 MscI TGG'CCA Cuts at: 0 697 1304 1419 Size: 697 607 115 Fragments arranged by size: 697 607 115 MspA1I CmG'CkG Cuts at: 0 468 1113 1155 1419 Size: 468 645 42 264 Fragments arranged by size: 645 468 264 42 NspI r_CATG'y Cuts at: 0 1110 1419 Size: 1110 309 PflMI CCAn_nnn'nTGG Cuts at: 0 1026 1419 Size: 1026 393 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 0 904 936 1419 Size: 904 32 483 Fragments arranged by size: 904 483 32 PpuMI rG'GwC_Cy Cuts at: 0 113 516 1419 Size: 113 403 903 Fragments arranged by size: 903 403 113 PshAI GACnn'nnGTC Cuts at: 0 491 1419 Size: 491 928 PstI C_TGCA'G Cuts at: 0 13 1043 1419 Size: 13 1030 376 Fragments arranged by size: 1030 376 13 PvuII CAG'CTG Cuts at: 0 1113 1155 1419 Size: 1113 42 264 Fragments arranged by size: 1113 264 42 SacI G_AGCT'C Cuts at: 0 1007 1390 1417 1419 Size: 1007 383 27 2 Fragments arranged by size: 1007 383 27 2 SexAI A'CCwGG_T Cuts at: 0 916 1419 Size: 916 503 SfcI C'TryA_G Cuts at: 0 9 489 1039 1419 Size: 9 480 550 380 Fragments arranged by size: 550 480 380 9 SmlI C'TyrA_G Cuts at: 0 1375 1419 Size: 1375 44 SphI G_CATG'C Cuts at: 0 1110 1419 Size: 1110 309 StuI AGG'CCT Cuts at: 0 728 1419 Size: 728 691 StyI C'CwwG_G Cuts at: 0 267 1419 Size: 267 1152 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 0 283 949 1419 Size: 283 666 470 Fragments arranged by size: 666 470 283 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 0 1270 1302 1419 Size: 1270 32 117 Fragments arranged by size: 1270 117 32 XcmI CCAnnnn_n'nnnnTGG Cuts at: 0 429 705 969 1132 1297 1419 Size: 429 276 264 163 165 122 Fragments arranged by size: 429 276 264 165 163 122 ZraI GAC'GTC Cuts at: 0 657 1419 Size: 657 762 Enzymes that do cut: AarI AatII Acc65I AcuI AlfI AlwNI BanI BanII BbsI BglI BglII BlpI Bme1580I BmrI BplI BpmI Bpu10I BpuEI BsaI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspMI BsrDI BsrFI BssSI BstEII BstYI Bsu36I BtsI EaeI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I FspI Hin4I KpnI MmeI MscI MspA1I NspI PflMI PpiI PpuMI PshAI PstI PvuII SacI SexAI SfcI SmlI SphI StuI StyI TaqII TstI XcmI ZraI Enzymes that do not cut: AccI AclI AfeI AflII AflIII AgeI AhdI AleI AloI ApaI ApaLI ApoI AscI AseI AsiSI AvaI AvrII BaeI BamHI BbeI BbvCI BcgI BciVI BclI BfrBI BmgBI BmtI BsaAI BsiEI BsiWI BspHI BsrBI BsrGI BssHII BstAPI BstBI BstXI BstZ17I BtgI BtgZI ClaI CspCI DraI DraIII DrdI EagI EcoRI EcoRV FalI FseI FspAI HaeII HincII HindIII HpaI KasI MfeI MluI MslI NaeI NarI NcoI NdeI NgoMIV NheI NotI NruI NsiI PacI PasI PciI PfoI PmeI PmlI PsiI PspOMI PspXI PsrI PvuI RsrII SacII SalI SanDI SapI SbfI ScaI SfiI SfoI SgfI SgrAI SmaI SnaBI SpeI SrfI SspI SwaI TatI Tth111I XbaI XhoI XmaI XmnI