################################## # NO CUT ENZYMES ( 113 enzymes) # ################################## AatII AclI AcsI AcyI AflII AflIII AgeI AhdI Aor51HI ApoI AscI AseI AsnI AspEI AspI AviII AvrII BbeI BbrPI BbvCI BcgI BciVI BclI BfrI BglII BlnI BsaAI BsaBI BsaHI BsiEI BsiWI BsmBI BsmI Bsp1407I BspDI BspHI BspLU11I BsrGI BssHII BssSI Bst1107I BstBI BstZ17I ClaI CpoI DraI DraIII DrdI EagI Eam1105I EclXI Eco47III Eco52I EcoRI EcoRV EcoT22I FbaI FseI FspI HgaI Hin1I HindIII HpaI KasI KspI MaeII MamI MfeI MluI MseI MslI MunI NarI NcoI NdeI NheI NotI NruI NsiI NspI NspV PacI PflFI PinAI PmaCI PmeI PmlI PshBI Psp1406I PvuI RcaI RsrII SacII SalI SapI SbfI ScaI SexAI SfaNI SfoI SfuI SgrAI SnaBI SpeI SphI Sse8387I SspBI SspI SwaI Tru9I Tth111I XbaI XcmI ################################### # ONE CUT ENZYMES ( 40 enzymes) # ################################### Acc65I 491 GGTACC G^GTACC AccI 211 GTCTAC GT^[AC][GT]AC AccIII 1263 TCCGGA T^CCGGA Alw44I 358 GTGCAC G^TGCAC ApaLI 358 GTGCAC G^TGCAC Asp700I 601 GAAAAGATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC Asp718I 491 GGTACC G^GTACC BamHI 938 GGATCC G^GATCC BbsI 1153 GAAGAC GAAGAC(2/6) BlpI 420 GCTCAGC GC^T[ACGT]AGC Bpu1102I 420 GCTCAGC GC^T[ACGT]AGC BpuAI 1153 GAAGAC GAAGAC(2/6) BsaI 1267 GAGACC GGTCTC(1/5) BsaWI 1263 TCCGGA [AT]^CCGG[AT] BseAI 1263 TCCGGA T^CCGGA BspEI 1263 TCCGGA T^CCGGA BsrDI 474 CATTGC GCAATG(2/0) BstAPI 1614 GCATAGCCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC BstEII 1254 GGTGACC G^GT[ACGT]ACC BstPI 1254 GGTGACC G^GT[ACGT]ACC BstXI 1576 CCACAAGGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG CelII 420 GCTCAGC GC^T[ACGT]AGC DsaI 647 CCGTGG C^C[GA][CT]GG EcoNI 1522 CCTAGATCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG EcoO65I 1254 GGTGACC G^GT[ACGT]ACC EcoT14I 1250 CCTTGG C^C[AT][AT]GG HincII 1132 GTCAAC GT[CT]^[GA]AC HindII 1132 GTCAAC GT[CT]^[GA]AC KpnI 491 GGTACC GGTAC^C MroI 1263 TCCGGA T^CCGGA NaeI 193 GCCGGC GCC^GGC NgoMIV 193 GCCGGC G^CCGGC PaeR7I 1127 CTCGAG C^TCGAG SfiI 65 GGCCCCCGGGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC SmlI 1127 CTCGAG C^T[CT][GA]AG StuI 483 AGGCCT AGG^CCT StyI 1250 CCTTGG C^C[AT][AT]GG Tsp509I 918 AATT ^AATT XhoI 1127 CTCGAG C^TCGAG XmnI 601 GAAAAGATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC ################################### # TWO CUT ENZYMES ( 27 enzymes) # ################################### AfaI 492 GTAC GT^AC 760 GTAC AocI 336 CCTAAGG CC^T[ACGT]AGG 1158 CCTCAGG BalI 866 TGGCCA TGG^CCA 1469 TGGCCA BglI 66 GCCCCCGGGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC 447 GCCGGTCAGGC BseRI 114 CTCCTC GAGGAG(10/8) 1810 CTCCTC BsrBI 1305 CCGCTC CCGCTC(-3/-3) 1642 GAGCGG BstYI 938 GGATCC [GA]^GATC[CT] 1044 AGATCC Bsu36I 336 CCTAAGG CC^T[ACGT]AGG 1158 CCTCAGG EarI 1075 CTCTTC CTCTTC(1/4) 1393 GAAGAG Eco81I 336 CCTAAGG CC^T[ACGT]AGG 1158 CCTCAGG HaeII 136 AGCGCC [GA]GCGC^[CT] 1446 AGCGCC Ksp632I 1075 CTCTTC CTCTTC(1/4) 1393 GAAGAG MflI 938 GGATCC [GA]^GATC[CT] 1044 AGATCC MluNI 866 TGGCCA TGG^CCA 1469 TGGCCA MscI 866 TGGCCA TGG^CCA 1469 TGGCCA PflMI 924 CCAGCACCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 1357 CCAGGTCCTGG PshAI 1125 GACTCGAGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC 1269 GACCCTGGTC PstI 764 CTGCAG CTGCA^G 1855 CTGCAG RsaI 492 GTAC GT^AC 760 GTAC SacI 745 GAGCTC GAGCT^C 1327 GAGCTC SfcI 764 CTGCAG C^T[GA][CT]AG 1855 CTGCAG TaqI 561 TCGA T^CGA 1128 TCGA TfiI 606 GATTC G^A[AT]TC 1096 GAATC Tsp45I 584 GTGAC ^GT[GC]AC 1255 GTGAC TthHB8I 561 TCGA T^CGA 1128 TCGA Van91I 924 CCAGCACCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 1357 CCAGGTCCTGG XhoII 938 GGATCC [GA]^GATC[CT] 1044 AGATCC ######################################### # 3 OR MORE CUT ENZYMES ( 78 enzymes) # ######################################### AccII AciI AluI AlwI AlwNI ApaI AspHI AvaI AvaII BanI BanII BbvI BcnI BfaI BmyI BpmI BsaJI BsgI BsiHKAI BsiYI BslI BsmAI BsmFI BsoBI Bsp1286I BspMI BsrFI BsrI BssKI BstF5I BstNI BstUI Cac8I CfoI Cfr10I Cfr13I DdeI DpnII DraII EaeI EcoO109I EcoRII FauI Fnu4HI FokI HaeIII HapII HhaI HinP1I HinfI HpaII HphI ItaI MaeI MaeIII MboI MboII MnlI MspA1I MspI MvaI MvnI MwoI NciI NdeII NlaIII NlaIV PleI PpuMI PvuII Sau3AI Sau96I ScrFI SmaI TseI TspRI XmaCI XmaI