Restriction map for
pFN21AB7793
##################################
# NO CUT ENZYMES ( 103 enzymes) #
##################################
Acc65I AccI AccIII AclI AflII
AgeI Alw44I AlwNI AocI Aor51HI
ApaI ApaLI AscI AseI AsnI
Asp718I AspHI AspI AviII AvrII
BbrPI BbvCI BciVI BclI BfrI
BglI BlnI BlpI Bpu1102I BsaAI
BsaBI BseAI BsiHKAI BsiWI Bsp1407I
BspDI BspEI BspHI BspLU11I BsrDI
BsrGI BssHII BssSI Bst1107I BstAPI
BstEII BstPI BstXI BstZ17I Bsu36I
CelII ClaI DraI DraIII Eco47III
Eco81I EcoNI EcoO65I EcoRI FbaI
FseI FspI HpaI KpnI MamI
MfeI MluI MroI MunI NcoI
NdeI NheI NotI NruI PacI
PflFI PinAI PmaCI PmeI PmlI
PshBI Psp1406I PvuI RcaI SacI
SalI SbfI ScaI SexAI SfiI
SmaI SnaBI SpeI SphI Sse8387I
SspBI SspI StuI SwaI Tth111I
XbaI XmaCI XmaI
###################################
# ONE CUT ENZYMES ( 68 enzymes) #
###################################
AatII 532 GACGTC GACGT^C
AflIII 1322 ACACGT A^C[GA][CT]GT
AhdI 532 GACGTCCAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Asp700I 943 GAAGCTGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AspEI 532 GACGTCCAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
BalI 1073 TGGCCA TGG^CCA
BamHI 1169 GGATCC G^GATCC
BanI 1216 GGCGCC G^G[CT][GA]CC
BanII 129 GGGCTC G[GA]GC[CT]^C
BbeI 1216 GGCGCC GGCGC^C
BbsI 529 GAAGAC GAAGAC(2/6)
BcgI 1040 CGAGTGACCTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BglII 844 AGATCT A^GATCT
BmyI 129 GGGCTC G[AGT]GC[ACT]^C
BpmI 1159 CTGGAG CTGGAG(16/14)
BpuAI 529 GAAGAC GAAGAC(2/6)
BsaI 17 GAGACC GGTCTC(1/5)
BsaWI 1121 ACCGGA [AT]^CCGG[AT]
BsiEI 436 CGGCCG CG[GA][CT]^CG
Bsp1286I 129 GGGCTC G[AGT]GC[ACT]^C
BspMI 1046 ACCTGC ACCTGC(4/8)
BsrBI 226 CCGCTC CCGCTC(-3/-3)
BstBI 729 TTCGAA TT^CGAA
CpoI 428 CGGTCCG CG^G[AT]CCG
DrdI 580 GACCAGTCTGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EagI 436 CGGCCG C^GGCCG
Eam1105I 532 GACGTCCAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EarI 818 GAAGAG CTCTTC(1/4)
EclXI 436 CGGCCG C^GGCCG
Eco52I 436 CGGCCG C^GGCCG
EcoRV 70 GATATC GAT^ATC
EcoT14I 141 CCAAGG C^C[AT][AT]GG
EcoT22I 791 ATGCAT ATGCA^T
HgaI 475 GACGC GACGC(5/10)
HindIII 43 AAGCTT A^AGCTT
KasI 1216 GGCGCC G^GCGCC
Ksp632I 818 GAAGAG CTCTTC(1/4)
KspI 887 CCGCGG CCGC^GG
MluNI 1073 TGGCCA TGG^CCA
MscI 1073 TGGCCA TGG^CCA
MseI 593 TTAA T^TAA
MslI 88 CACGATGGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
NarI 1216 GGCGCC GG^CGCC
NsiI 791 ATGCAT ATGCA^T
NspI 662 GCATGT [GA]CATG^[CT]
NspV 729 TTCGAA TT^CGAA
PaeR7I 153 CTCGAG C^TCGAG
PflMI 465 CCACGGCCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PleI 809 GAGTC GAGTC(4/5)
PshAI 764 GACGGCTGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
PstI 1249 CTGCAG CTGCA^G
PvuII 124 CAGCTG CAG^CTG
RsrII 428 CGGTCCG CG^G[AT]CCG
SacII 887 CCGCGG CCGC^GG
SapI 818 GAAGAGC GCTCTTC(1/4)
SfcI 1249 CTGCAG C^T[GA][CT]AG
SfoI 1216 GGCGCC GGC^GCC
SfuI 729 TTCGAA TT^CGAA
SgrAI 675 CACCGGCG C[GA]^CCGG[CT]G
SmlI 153 CTCGAG C^T[CT][GA]AG
StyI 141 CCAAGG C^C[AT][AT]GG
TfiI 570 GAATC G^A[AT]TC
Tru9I 593 TTAA T^TAA
TspRI 290 CACTG CA[GC]TG[ACGT][ACGT]^
Van91I 465 CCACGGCCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XcmI 1148 CCAGCCCCATCCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XhoI 153 CTCGAG C^TCGAG
XmnI 943 GAAGCTGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 19 enzymes) #
###################################
AcsI 879 GAATTT [GA]^AATT[CT]
1096 GAATTT
AcyI 532 GACGTC G[GA]^CG[CT]C
1216 GGCGCC
ApoI 879 GAATTT [GA]^AATT[CT]
1096 GAATTT
BsaHI 532 GACGTC G[GA]^CG[CT]C
1216 GGCGCC
BseRI 799 CTCCTC GAGGAG(10/8)
1363 GAGGAG
BsmBI 1005 CGTCTC CGTCTC(1/5)
762 GAGACG
BsmI 781 GAATGC GAATGC(1/-1)
453 GCATTC
DraII 144 AGGACCC [GA]G^G[ACGT]CC[CT]
248 AGGACCC
DsaI 465 CCACGG C^C[GA][CT]GG
887 CCGCGG
EcoO109I 144 AGGACCC [GA]G^G[ACGT]CC[CT]
248 AGGACCC
Hin1I 532 GACGTC G[GA]^CG[CT]C
1216 GGCGCC
HincII 265 GTCAAC GT[CT]^[GA]AC
670 GTCAAC
HindII 265 GTCAAC GT[CT]^[GA]AC
670 GTCAAC
HinfI 570 GAATC G^A[ACGT]TC
809 GAGTC
NaeI 352 GCCGGC GCC^GGC
434 GCCGGC
NgoMIV 352 GCCGGC G^CCGGC
434 GCCGGC
PpuMI 144 AGGACCC [GA]G^G[AT]CC[CT]
248 AGGACCC
SfaNI 550 GATGC GCATC(5/9)
793 GCATC
Tsp509I 880 AATT ^AATT
1097 AATT
#########################################
# 3 OR MORE CUT ENZYMES ( 68 enzymes) #
#########################################
AccII AciI AfaI AluI AlwI
AvaI AvaII BbvI BcnI BfaI
BsaJI BsgI BsiYI BslI BsmAI
BsmFI BsoBI BsrFI BsrI BssKI
BstF5I BstNI BstUI BstYI Cac8I
CfoI Cfr10I Cfr13I DdeI DpnII
EaeI EcoRII FauI Fnu4HI FokI
HaeII HaeIII HapII HhaI HinP1I
HpaII HphI ItaI MaeI MaeII
MaeIII MboI MboII MflI MnlI
MspA1I MspI MvaI MvnI MwoI
NciI NdeII NlaIII NlaIV RsaI
Sau3AI Sau96I ScrFI TaqI TseI
Tsp45I TthHB8I XhoII