(Circular) (Six-base) MAPSORT of: pFN21AB7793.seq Check: 7284 from: 1 to: 6085 pFN21AB7793 6085 bp With 182 enzymes: SgfI * April 15, 2010 09:34 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 2541 3895 278 Size: 53 83 186 936 1005 1354 2468 Fragments arranged by size: 2468 1354 1005 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3428 1937 Size: 1491 4594 Acc65I G'GTAC_C Cuts at: 3406 3406 Size: 6085 AclI AA'CG_TT Cuts at: 1563 4212 4585 1563 Size: 2649 373 3063 Fragments arranged by size: 3063 2649 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 3181 4148 5532 5800 1632 Size: 1549 967 1384 268 1917 Fragments arranged by size: 1917 1549 1384 967 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5888 AflIII A'CryG_T Cuts at: 1203 3327 4984 5961 1203 Size: 2124 1657 977 1327 Fragments arranged by size: 2124 1657 1327 977 AgeI A'CCGG_T Cuts at: 4916 4916 Size: 6085 AhdI GACnn_n'nnGTC Cuts at: 2542 4814 2542 Size: 2272 3813 AleI CACnn'nnGTG Cuts at: 1166 2097 1166 Size: 931 5154 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 6085 AloI GAACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2937 2969 3281 3313 2937 Size: 32 312 32 5709 Fragments arranged by size: 5709 312 32 32 AlwNI CAG_nnn'CTG Cuts at: 3828 5400 3828 Size: 1572 4513 ApaLI G'TGCA_C Cuts at: 4142 5298 4142 Size: 1156 4929 ApoI r'AATT_y Cuts at: 1493 2884 3101 3394 3620 3772 5881 1493 Size: 1391 217 293 226 152 2109 1697 Fragments arranged by size: 2109 1697 1391 293 226 217 152 AseI AT'TA_AT Cuts at: 160 1032 4639 160 Size: 872 3607 1606 Fragments arranged by size: 3607 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6085 AvaI C'yCGr_G Cuts at: 1870 1943 2104 2158 2379 2401 3043 3410 1870 Size: 73 161 54 221 22 642 367 4545 Fragments arranged by size: 4545 642 367 221 161 73 54 22 BamHI G'GATC_C Cuts at: 3174 3415 3782 3174 Size: 241 367 5477 Fragments arranged by size: 5477 367 241 BanI G'GyrC_C Cuts at: 618 943 1160 1787 3221 3406 4861 618 Size: 325 217 627 1434 185 1455 1842 Fragments arranged by size: 1842 1455 1434 627 325 217 185 BanII G_rGCy'C Cuts at: 1391 2138 3404 1391 Size: 747 1266 4072 Fragments arranged by size: 4072 1266 747 BbeI G_GCGC'C Cuts at: 3225 3225 Size: 6085 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2541 928 Size: 971 642 4472 Fragments arranged by size: 4472 971 642 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 1987 2021 3034 3068 4276 4310 1903 Size: 34 50 34 1013 34 1208 34 3678 Fragments arranged by size: 3678 1208 1013 50 34 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3977 5187 3977 Size: 1210 4875 BclI T'GATC_A Cuts at: 1548 1548 Size: 6085 BfrBI ATG'CAT Cuts at: 2798 2798 Size: 6085 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 4696 136 Size: 107 122 71 631 605 3024 1525 Fragments arranged by size: 3024 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 2849 6080 2849 Size: 3231 2854 BlpI GC'TnA_GC Cuts at: 3502 3502 Size: 6085 Bme1580I G_kGCm'C Cuts at: 1790 4146 5302 1790 Size: 2356 1156 2573 Fragments arranged by size: 2573 2356 1156 BmgBI CAC'GTC Cuts at: 1318 2269 2449 3330 1318 Size: 951 180 881 4073 Fragments arranged by size: 4073 951 881 180 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3816 4774 442 Size: 416 53 83 399 24 2399 958 1753 Fragments arranged by size: 2399 1753 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6085 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 3185 4745 1239 Size: 1946 1560 2579 Fragments arranged by size: 2579 1946 1560 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 4210 5075 5373 4210 Size: 865 298 4922 Fragments arranged by size: 4922 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2016 4748 882 Size: 617 517 2732 2219 Fragments arranged by size: 2732 2219 617 517 BsaAI yAC'GTr Cuts at: 493 1204 3876 493 Size: 711 2672 2702 Fragments arranged by size: 2702 2672 711 BsaBI GATnn'nnATC Cuts at: 6079 6079 Size: 6085 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2538 3222 3892 4274 Size: 53 83 186 936 1005 684 670 382 Cuts at: 4274 4909 275 Size: 635 1451 Fragments arranged by size: 1451 1005 936 684 670 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 3126 4518 4916 5190 5337 5748 3126 Size: 1392 398 274 147 411 3463 Fragments arranged by size: 3463 1392 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2246 2267 2276 2297 2937 Size: 30 165 30 843 21 9 21 640 Cuts at: 2937 2967 3041 3071 1178 Size: 30 74 30 4192 Fragments arranged by size: 4192 843 640 165 74 30 30 30 30 21 21 9 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2795 3383 1186 Size: 1609 588 3888 Fragments arranged by size: 3888 1609 588 BseYI C'CCAG_C Cuts at: 2127 2131 2307 2373 2394 3152 5288 5784 2127 Size: 4 176 66 21 758 2136 496 2428 Fragments arranged by size: 2428 2136 758 496 176 66 21 4 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2282 2492 3038 1163 Size: 573 546 210 546 4210 Fragments arranged by size: 4210 573 546 546 210 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2444 3535 4296 5324 1540 Size: 275 186 443 1091 761 1028 2301 Fragments arranged by size: 2301 1091 1028 761 443 275 186 BsiHKAI G_wGCw'C Cuts at: 4146 4231 5302 4146 Size: 85 1071 4929 Fragments arranged by size: 4929 1071 85 BsmI GAATG_Cn' Cuts at: 2458 2792 3596 3689 2458 Size: 334 804 93 4854 Fragments arranged by size: 4854 804 334 93 BsmBI CGTCTCn'nnnn_ Cuts at: 2761 3016 2761 Size: 255 5830 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2138 3404 4146 4231 5302 1391 Size: 399 348 1266 742 85 1071 2174 Fragments arranged by size: 2174 1266 1071 742 399 348 85 BspEI T'CCGG_A Cuts at: 5748 5748 Size: 6085 BspHI T'CATG_A Cuts at: 3974 5704 3974 Size: 1730 4355 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 3060 3440 844 Size: 2216 380 3489 Fragments arranged by size: 3489 2216 380 BsrBI CCG'CTC Cuts at: 1394 1823 2233 3972 1394 Size: 429 410 1739 3507 Fragments arranged by size: 3507 1739 429 410 BsrDI GCAATG_nn' Cuts at: 65 1248 4580 4754 65 Size: 1183 3332 174 1396 Fragments arranged by size: 3332 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2088 2357 2439 2681 3027 4729 4916 Size: 110 278 269 82 242 346 1702 187 Cuts at: 4916 1700 Size: 2869 Fragments arranged by size: 2869 1702 346 278 269 242 187 110 82 BsrGI T'GTAC_A Cuts at: 96 96 Size: 6085 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6085 BssSI C'ACGA_G Cuts at: 4145 5157 4145 Size: 1012 5073 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 6085 BstBI TT'CG_AA Cuts at: 2735 4889 2735 Size: 2154 3931 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 6085 BstYI r'GATC_y Cuts at: 1972 2849 3109 3174 3415 3782 4167 4184 5636 Size: 877 260 65 241 367 385 17 1452 Cuts at: 5636 5722 5734 6080 1972 Size: 86 12 346 1977 Fragments arranged by size: 1977 1452 877 385 367 346 260 241 86 65 17 12 BtgI C'CryG_G Cuts at: 513 1065 2470 2892 513 Size: 552 1405 422 3706 Fragments arranged by size: 3706 1405 552 422 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5346 Fragments arranged by size: 5346 683 56 BtsI GCAGTG_nn' Cuts at: 2294 3601 4395 4415 2294 Size: 1307 794 20 3964 Fragments arranged by size: 3964 1307 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6085 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 6050 DraI TTT'AAA Cuts at: 3389 3389 Size: 6085 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 2591 5092 809 Size: 507 1275 2501 1802 Fragments arranged by size: 2501 1802 1275 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2441 2518 3078 Size: 54 1425 50 275 18 611 77 560 Cuts at: 3078 3228 3532 4421 6050 8 Size: 150 304 889 1629 43 Fragments arranged by size: 1629 1425 889 611 560 304 275 150 77 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 2441 3532 1537 Size: 275 629 1091 4090 Fragments arranged by size: 4090 1091 629 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2818 4015 1353 Size: 1465 1197 3423 Fragments arranged by size: 3423 1465 1197 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2426 2826 4672 5046 5192 387 Size: 1064 138 837 400 1846 374 146 1280 Fragments arranged by size: 1846 1280 1064 837 400 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 3181 3185 4148 4745 5532 5800 1239 Size: 393 1549 4 963 597 787 268 1524 Fragments arranged by size: 1549 1524 963 787 597 393 268 4 EcoO109I rG'GnC_Cy Cuts at: 2150 2254 2150 Size: 104 5981 EcoRI G'AATT_C Cuts at: 3394 3772 3394 Size: 378 5707 EcoRV GAT'ATC Cuts at: 2077 2077 Size: 6085 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 3316 3316 Size: 6085 FspI TGC'GCA Cuts at: 4591 4591 Size: 6085 HaeII r_GCGC'y Cuts at: 1678 2166 3225 3278 5232 1678 Size: 488 1059 53 1954 2531 Fragments arranged by size: 2531 1954 1059 488 53 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2246 2267 2278 2299 2414 Size: 32 135 32 674 21 11 21 115 Cuts at: 2414 2446 2486 2518 2528 2560 3041 3073 4726 Size: 32 40 32 10 32 481 32 1653 Cuts at: 4726 4758 4800 4832 1373 Size: 32 42 32 2626 Fragments arranged by size: 2626 1653 674 481 135 115 42 40 32 32 32 32 32 32 32 32 21 21 11 10 HincII GTy'rAC Cuts at: 669 1657 1938 2272 2677 3429 3675 669 Size: 988 281 334 405 752 246 3079 Fragments arranged by size: 3079 988 752 405 334 281 246 HindIII A'AGCT_T Cuts at: 748 2048 748 Size: 1300 4785 HpaI GTT'AAC Cuts at: 3675 3675 Size: 6085 KasI G'GCGC_C Cuts at: 3221 3221 Size: 6085 KpnI G_GTAC'C Cuts at: 3410 3410 Size: 6085 MfeI C'AATT_G Cuts at: 3684 3684 Size: 6085 MluI A'CGCG_T Cuts at: 5961 5961 Size: 6085 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2172 5199 5383 1305 Size: 867 3027 184 2007 Fragments arranged by size: 3027 2007 867 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 3080 10 Size: 54 1425 343 1248 3015 Fragments arranged by size: 3015 1425 1248 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2097 4043 4402 4561 518 Size: 648 931 1946 359 159 2042 Fragments arranged by size: 2042 1946 931 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2131 2173 2335 2568 2894 3501 4178 5326 Size: 525 42 162 233 326 607 677 1148 Cuts at: 5326 5571 1606 Size: 245 2120 Fragments arranged by size: 2120 1148 677 607 525 326 245 233 162 42 NaeI GCC'GGC Cuts at: 2359 2441 2359 Size: 82 6003 NarI GG'CG_CC Cuts at: 3222 3222 Size: 6085 NcoI C'CATG_G Cuts at: 513 1065 513 Size: 552 5533 NdeI CA'TA_TG Cuts at: 387 387 Size: 6085 NgoMIV G'CCGG_C Cuts at: 2357 2439 2357 Size: 82 6003 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6085 NotI GC'GGCC_GC Cuts at: 3532 3532 Size: 6085 NsiI A_TGCA'T Cuts at: 2800 2800 Size: 6085 NspI r_CATG'y Cuts at: 1132 1229 2671 3443 4988 1132 Size: 97 1442 772 1545 2229 Fragments arranged by size: 2229 1545 1442 772 97 PasI CC'CwG_GG Cuts at: 1348 2319 1348 Size: 971 5114 PciI A'CATG_T Cuts at: 4984 4984 Size: 6085 PflMI CCAn_nnn'nTGG Cuts at: 1108 2476 1108 Size: 1368 4717 PfoI T'CCnGG_A Cuts at: 2524 3162 2524 Size: 638 5447 PmeI GTTT'AAAC Cuts at: 3389 3389 Size: 6085 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2937 2969 4154 4186 5667 5699 2937 Size: 32 1185 32 1481 32 3323 Fragments arranged by size: 3323 1481 1185 32 32 32 PpuMI rG'GwC_Cy Cuts at: 2150 2254 2150 Size: 104 5981 PshAI GACnn'nnGTC Cuts at: 1613 2773 1613 Size: 1160 4925 PsiI TTA'TAA Cuts at: 3655 3655 Size: 6085 PspXI vC'TCGA_Gb Cuts at: 2158 2158 Size: 6085 PstI C_TGCA'G Cuts at: 830 3258 3437 830 Size: 2428 179 3478 Fragments arranged by size: 3478 2428 179 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6085 PvuII CAG'CTG Cuts at: 2131 2131 Size: 6085 RsrII CG'GwC_CG Cuts at: 2434 2434 Size: 6085 SacII CC_GC'GG Cuts at: 2895 2895 Size: 6085 SalI G'TCGA_C Cuts at: 1936 3427 1936 Size: 1491 4594 SapI GCTCTTCn'nnn_ Cuts at: 2818 2818 Size: 6085 SbfI CC_TGCA'GG Cuts at: 3437 3437 Size: 6085 ScaI AGT'ACT Cuts at: 4333 6045 4333 Size: 1712 4373 SfcI C'TryA_G Cuts at: 826 1045 3254 3433 4568 5249 5440 826 Size: 219 2209 179 1135 681 191 1471 Fragments arranged by size: 2209 1471 1135 681 219 191 179 SfoI GGC'GCC Cuts at: 3223 3223 Size: 6085 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6085 SgrAI Cr'CCGG_yG Cuts at: 1700 2681 1700 Size: 981 5104 SmaI CCC'GGG Cuts at: 1872 3412 1872 Size: 1540 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2158 4189 5090 5352 820 Size: 197 926 215 2031 901 262 1553 Fragments arranged by size: 2031 1553 926 901 262 215 197 SnaBI TAC'GTA Cuts at: 493 3876 493 Size: 3383 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 6085 SphI G_CATG'C Cuts at: 1132 3443 1132 Size: 2311 3774 SspI AAT'ATT Cuts at: 5 52 4009 5 Size: 47 3957 2081 Fragments arranged by size: 3957 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 6085 StyI C'CwwG_G Cuts at: 513 1065 2146 3524 513 Size: 552 1081 1378 3074 Fragments arranged by size: 3074 1378 1081 552 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2422 2480 4112 4129 4282 4467 4912 Size: 1255 842 58 1632 17 153 185 445 Cuts at: 4912 325 Size: 1498 Fragments arranged by size: 1632 1498 1255 842 445 185 153 58 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2739 4331 6043 Size: 274 80 33 51 1443 762 1592 1712 Cuts at: 6043 96 Size: 138 Fragments arranged by size: 1712 1592 1443 762 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 6045 6077 6045 Size: 32 6053 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 6085 XbaI T'CTAG_A Cuts at: 3421 3421 Size: 6085 XcmI CCAnnnn_n'nnnnTGG Cuts at: 3160 3160 Size: 6085 XhoI C'TCGA_G Cuts at: 1943 2158 1943 Size: 215 5870 XmaI C'CCGG_G Cuts at: 1870 3410 1870 Size: 1540 4545 XmnI GAAnn'nnTTC Cuts at: 2952 4214 2952 Size: 1262 4823 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 2539 3893 276 Size: 53 83 186 936 1005 1354 2468 Fragments arranged by size: 2468 1354 1005 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AloI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI BcgI BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoO109I EcoRI EcoRV FalI FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NarI NcoI NdeI NgoMIV NheI NotI NsiI NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PspXI PstI PvuI PvuII RsrII SacII SalI SapI SbfI ScaI SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI ApaI AscI AvrII BaeI BbvCI BplI Bpu10I BsiWI BstXI BstZ17I Bsu36I DraIII EcoICRI EcoNI FseI FspAI NruI PacI PmlI PspOMI PsrI SacI SanDI SexAI SfiI SrfI SwaI