Restriction map for
pFN21AB7793
(Linear) (Six-base) MAPSORT of: KIBB7793.seq Check: 533 from: 1 to: 1380
KIBB7793.orf
With 182 enzymes: SgfI *
October 7, 2009 14:24 ..
AatII G_ACGT'C
Cuts at: 0 536 1380
Size: 536 844
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1176 1380
Size: 1176 204
AflIII A'CryG_T
Cuts at: 0 1322 1380
Size: 1322 58
AhdI GACnn_n'nnGTC
Cuts at: 0 537 1380
Size: 537 843
AleI CACnn'nnGTG
Cuts at: 0 92 1380
Size: 92 1288
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 932 964 1276 1308 1380
Size: 932 32 312 32 72
Fragments arranged by size:
932 312 72 32 32
ApoI r'AATT_y
Cuts at: 0 879 1096 1380
Size: 879 217 284
Fragments arranged by size:
879 284 217
AvaI C'yCGr_G
Cuts at: 0 99 153 374 396 1038 1380
Size: 99 54 221 22 642 342
Fragments arranged by size:
642 342 221 99 54 22
BamHI G'GATC_C
Cuts at: 0 1169 1380
Size: 1169 211
BanI G'GyrC_C
Cuts at: 0 1216 1380
Size: 1216 164
BanII G_rGCy'C
Cuts at: 0 133 1380
Size: 133 1247
BbeI G_GCGC'C
Cuts at: 0 1220 1380
Size: 1220 160
BbsI GAAGACnn'nnnn_
Cuts at: 0 536 1380
Size: 536 844
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 1029 1063 1380
Size: 1029 34 317
Fragments arranged by size:
1029 317 34
BfrBI ATG'CAT
Cuts at: 0 793 1380
Size: 793 587
BglII A'GATC_T
Cuts at: 0 844 1380
Size: 844 536
BmgBI CAC'GTC
Cuts at: 0 264 444 1325 1380
Size: 264 180 881 55
Fragments arranged by size:
881 264 180 55
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1180 1380
Size: 1180 200
BsaI GGTCTCn'nnnn_
Cuts at: 0 11 1380
Size: 11 1369
BsaHI Gr'CG_yC
Cuts at: 0 533 1217 1380
Size: 533 684 163
Fragments arranged by size:
684 533 163
BsaWI w'CCGG_w
Cuts at: 0 1121 1380
Size: 1121 259
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 241 262 271 292 932 962 1036 1066
Size: 241 21 9 21 640 30 74 30
Cuts at: 1066 1380
Size: 314
Fragments arranged by size:
640 314 241 74 30 30 21 21
9
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 790 1378 1380
Size: 790 588 2
Fragments arranged by size:
790 588 2
BseYI C'CCAG_C
Cuts at: 0 122 126 302 368 389 1147 1380
Size: 122 4 176 66 21 758 233
Fragments arranged by size:
758 233 176 122 66 21 4
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 277 487 1033 1380
Size: 277 210 546 347
Fragments arranged by size:
546 347 277 210
BsiEI CG_ry'CG
Cuts at: 0 439 1380
Size: 439 941
BsmI GAATG_Cn'
Cuts at: 0 453 787 1380
Size: 453 334 593
Fragments arranged by size:
593 453 334
BsmBI CGTCTCn'nnnn_
Cuts at: 0 756 1011 1380
Size: 756 255 369
Fragments arranged by size:
756 369 255
Bsp1286I G_dGCh'C
Cuts at: 0 133 1380
Size: 133 1247
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 1055 1380
Size: 1055 325
BsrBI CCG'CTC
Cuts at: 0 228 1380
Size: 228 1152
BsrFI r'CCGG_y
Cuts at: 0 83 352 434 676 1022 1380
Size: 83 269 82 242 346 358
Fragments arranged by size:
358 346 269 242 83 82
BstBI TT'CG_AA
Cuts at: 0 730 1380
Size: 730 650
BstYI r'GATC_y
Cuts at: 0 844 1104 1169 1380
Size: 844 260 65 211
Fragments arranged by size:
844 260 211 65
BtgI C'CryG_G
Cuts at: 0 465 887 1380
Size: 465 422 493
Fragments arranged by size:
493 465 422
BtsI GCAGTG_nn'
Cuts at: 0 289 1380
Size: 289 1091
DrdI GACnn_nn'nnGTC
Cuts at: 0 586 1380
Size: 586 794
EaeI y'GGCC_r
Cuts at: 0 436 513 1073 1223 1380
Size: 436 77 560 150 157
Fragments arranged by size:
560 436 157 150 77
EagI C'GGCC_G
Cuts at: 0 436 1380
Size: 436 944
EarI CTCTTCn'nnn_
Cuts at: 0 813 1380
Size: 813 567
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 421 821 1380
Size: 421 400 559
Fragments arranged by size:
559 421 400
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1176 1180 1380
Size: 1176 4 200
Fragments arranged by size:
1176 200 4
EcoO109I rG'GnC_Cy
Cuts at: 0 145 249 1380
Size: 145 104 1131
Fragments arranged by size:
1131 145 104
EcoRV GAT'ATC
Cuts at: 0 72 1380
Size: 72 1308
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 1311 1380
Size: 1311 69
HaeII r_GCGC'y
Cuts at: 0 161 1220 1273 1380
Size: 161 1059 53 107
Fragments arranged by size:
1059 161 107 53
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 241 262 273 294 409 441 481 513
Size: 241 21 11 21 115 32 40 32
Cuts at: 513 523 555 1036 1068 1380
Size: 10 32 481 32 312
Fragments arranged by size:
481 312 241 115 40 32 32 32
32 21 21 11 10
HincII GTy'rAC
Cuts at: 0 267 672 1380
Size: 267 405 708
Fragments arranged by size:
708 405 267
HindIII A'AGCT_T
Cuts at: 0 43 1380
Size: 43 1337
KasI G'GCGC_C
Cuts at: 0 1216 1380
Size: 1216 164
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 167 1380
Size: 167 1213
MscI TGG'CCA
Cuts at: 0 1075 1380
Size: 1075 305
MslI CAynn'nnrTG
Cuts at: 0 92 1380
Size: 92 1288
MspA1I CmG'CkG
Cuts at: 0 126 168 330 563 889 1380
Size: 126 42 162 233 326 491
Fragments arranged by size:
491 326 233 162 126 42
NaeI GCC'GGC
Cuts at: 0 354 436 1380
Size: 354 82 944
Fragments arranged by size:
944 354 82
NarI GG'CG_CC
Cuts at: 0 1217 1380
Size: 1217 163
NgoMIV G'CCGG_C
Cuts at: 0 352 434 1380
Size: 352 82 946
Fragments arranged by size:
946 352 82
NsiI A_TGCA'T
Cuts at: 0 795 1380
Size: 795 585
NspI r_CATG'y
Cuts at: 0 666 1380
Size: 666 714
PasI CC'CwG_GG
Cuts at: 0 314 1380
Size: 314 1066
PflMI CCAn_nnn'nTGG
Cuts at: 0 471 1380
Size: 471 909
PfoI T'CCnGG_A
Cuts at: 0 519 1157 1380
Size: 519 638 223
Fragments arranged by size:
638 519 223
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 932 964 1380
Size: 932 32 416
Fragments arranged by size:
932 416 32
PpuMI rG'GwC_Cy
Cuts at: 0 145 249 1380
Size: 145 104 1131
Fragments arranged by size:
1131 145 104
PshAI GACnn'nnGTC
Cuts at: 0 768 1380
Size: 768 612
PspXI vC'TCGA_Gb
Cuts at: 0 153 1380
Size: 153 1227
PstI C_TGCA'G
Cuts at: 0 1253 1380
Size: 1253 127
PvuII CAG'CTG
Cuts at: 0 126 1380
Size: 126 1254
RsrII CG'GwC_CG
Cuts at: 0 429 1380
Size: 429 951
SacII CC_GC'GG
Cuts at: 0 890 1380
Size: 890 490
SapI GCTCTTCn'nnn_
Cuts at: 0 813 1380
Size: 813 567
SfcI C'TryA_G
Cuts at: 0 1249 1380
Size: 1249 131
SfoI GGC'GCC
Cuts at: 0 1218 1380
Size: 1218 162
SgrAI Cr'CCGG_yG
Cuts at: 0 676 1380
Size: 676 704
SmlI C'TyrA_G
Cuts at: 0 153 1380
Size: 153 1227
StyI C'CwwG_G
Cuts at: 0 141 1380
Size: 141 1239
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 417 475 1380
Size: 417 58 905
Fragments arranged by size:
905 417 58
TatI w'GTAC_w
Cuts at: 0 734 1380
Size: 734 646
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 1155 1380
Size: 1155 225
XhoI C'TCGA_G
Cuts at: 0 153 1380
Size: 153 1227
XmnI GAAnn'nnTTC
Cuts at: 0 947 1380
Size: 947 433
ZraI GAC'GTC
Cuts at: 0 534 1380
Size: 534 846
Enzymes that do cut:
AatII AcuI AflIII AhdI AleI AloI ApoI AvaI
BamHI BanI BanII BbeI BbsI BcgI BfrBI BglII
BmgBI BpmI BsaI BsaHI BsaWI BsaXI BseRI BseYI
BsgI BsiEI BsmI BsmBI Bsp1286I BspMI BsrBI BsrFI
BstBI BstYI BtgI BtsI DrdI EaeI EagI EarI
EciI Eco57MI EcoO109I EcoRV FalI HaeII Hin4I HincII
HindIII KasI MmeI MscI MslI MspA1I NaeI NarI
NgoMIV NsiI NspI PasI PflMI PfoI PpiI PpuMI
PshAI PspXI PstI PvuII RsrII SacII SapI SfcI
SfoI SgrAI SmlI StyI TaqII TatI XcmI XhoI
XmnI ZraI
Enzymes that do not cut:
AarI AccI Acc65I AclI AfeI AflII AgeI AlfI
AlwNI ApaI ApaLI AscI AseI AsiSI AvrII BaeI
BbvCI BciVI BclI BglI BlpI Bme1580I BmrI BmtI
BplI Bpu10I BpuEI BsaAI BsaBI BsiHKAI BsiWI BspEI
BspHI BsrDI BsrGI BssHII BssSI BstAPI BstEII BstXI
BstZ17I Bsu36I BtgZI ClaI CspCI DraI DraIII EcoICRI
EcoNI EcoRI FseI FspI FspAI HpaI KpnI MfeI
MluI NcoI NdeI NheI NotI NruI PacI PciI
PmeI PmlI PsiI PspOMI PsrI PvuI SacI SalI
SanDI SbfI ScaI SexAI SfiI SgfI SmaI SnaBI
SpeI SphI SrfI SspI StuI SwaI TstI Tth111I
XbaI XmaI