Restriction map for
pFN21AE0216
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# NO CUT ENZYMES ( 159 enzymes) #
##################################
AatII Acc65I AccI AccIII AclI
AcsI AflII AgeI AhdI Alw44I
AocI Aor51HI ApaLI ApoI AscI
AseI AsnI Asp700I Asp718I AspEI
AspI AvaII AviII AvrII BalI
BamHI BbrPI BbsI BbvCI BcgI
BciVI BclI BfaI BfrI BglII
BlnI BlpI Bpu1102I BpuAI BsaAI
BsaBI BsaWI BseAI BsgI BsiEI
BsiWI BsmI Bsp1407I BspDI BspEI
BspLU11I BspMI BsrBI BsrDI BsrGI
BssHII BssSI Bst1107I BstAPI BstBI
BstEII BstPI BstXI BstYI BstZ17I
Bsu36I CelII ClaI CpoI DdeI
DraI DraIII DrdI DsaI EaeI
EagI Eam1105I EclXI Eco47III Eco52I
Eco81I EcoO65I EcoRI EcoRV EcoT22I
FbaI FseI FspI HgaI HincII
HindII HindIII HpaI HphI KpnI
KspI MaeI MamI MfeI MflI
MluI MluNI MroI MscI MseI
MunI NcoI NdeI NheI NotI
NsiI NspV PacI PaeR7I PflFI
PflMI PinAI PmaCI PmeI PmlI
PpuMI PshAI PshBI Psp1406I PstI
PvuI RsrII SacI SacII SalI
SapI SbfI ScaI SexAI SfcI
SfiI SfuI SgrAI SmaI SmlI
SnaBI SpeI SphI Sse8387I SspBI
SspI SwaI TfiI Tru9I Tsp509I
Tth111I Van91I XbaI XcmI XhoI
XhoII XmaCI XmaI XmnI
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# ONE CUT ENZYMES ( 42 enzymes) #
###################################
AccII 397 CGCG CG^CG
AcyI 51 GGCGCC G[GA]^CG[CT]C
AfaI 105 GTAC GT^AC
AflIII 233 ACGTGT A^C[GA][CT]GT
AlwNI 462 CAGTCACTG CAG[ACGT][ACGT][ACGT]^CTG
ApaI 188 GGGCCC GGGCC^C
AvaI 403 CCCGAG C^[CT]CG[GA]G
BanI 51 GGCGCC G^G[CT][GA]CC
BbeI 51 GGCGCC GGCGC^C
BcnI 98 CCGGG CC^[GC]GG
BglI 293 GCCAGGGCGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BsaHI 51 GGCGCC G[GA]^CG[CT]C
BsaI 496 GGTCTC GGTCTC(1/5)
BseRI 412 GAGGAG GAGGAG(10/8)
BsmBI 258 CGTCTC CGTCTC(1/5)
BsoBI 403 CCCGAG C^[CT]CG[GA]G
BspHI 23 TCATGA T^CATGA
BstF5I 213 CATCC GGATG(2/0)
BstUI 397 CGCG CG^CG
EarI 427 GAAGAG CTCTTC(1/4)
EcoNI 159 CCTGTTTGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
FokI 213 CATCC GGATG(9/13)
Hin1I 51 GGCGCC G[GA]^CG[CT]C
HinfI 357 GACTC G^A[ACGT]TC
KasI 51 GGCGCC G^GCGCC
Ksp632I 427 GAAGAG CTCTTC(1/4)
MaeIII 464 GTCAC ^GT[ACGT]AC
MslI 213 CATCCAGGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
MvnI 397 CGCG CG^CG
NarI 51 GGCGCC GG^CGCC
NciI 98 CCGGG CC^[GC]GG
NruI 396 TCGCGA TCG^CGA
NspI 44 ACATGC [GA]CATG^[CT]
PleI 357 GACTC GAGTC(4/5)
PvuII 148 CAGCTG CAG^CTG
RcaI 23 TCATGA T^CATGA
RsaI 105 GTAC GT^AC
SfaNI 438 GATGC GCATC(5/9)
SfoI 51 GGCGCC GGC^GCC
TaqI 533 TCGA T^CGA
Tsp45I 464 GTCAC ^GT[GC]AC
TthHB8I 533 TCGA T^CGA
###################################
# TWO CUT ENZYMES ( 21 enzymes) #
###################################
AluI 149 AGCT AG^CT
328 AGCT
AlwI 400 GATCC GGATC(4/5)
371 GGATC
AspHI 133 GAGCAC G[AT]GC[AT]^C
220 GTGCTC
BanII 188 GGGCCC G[GA]GC[CT]^C
526 GAGCCC
BbvI 150 GCTGC GCAGC(8/12)
555 GCTGC
BsiHKAI 133 GAGCAC G[AT]GC[AT]^C
220 GTGCTC
BsrFI 48 GCCGGC [GA]^CCGG[CT]
75 GCCGGC
BsrI 561 ACTGG ACTGG(1/-1)
461 CCAGT
Cfr10I 48 GCCGGC [GA]^CCGG[CT]
75 GCCGGC
DraII 187 GGGGCCC [GA]G^G[ACGT]CC[CT]
188 GGGCCCT
EcoO109I 187 GGGGCCC [GA]G^G[ACGT]CC[CT]
188 GGGCCCT
EcoT14I 29 CCAAGG C^C[AT][AT]GG
501 CCTTGG
FauI 290 CCCGC CCCGC(4/6)
448 CCCGC
HaeII 51 GGCGCC [GA]GCGC^[CT]
473 AGCGCC
MboII 424 GAAGA GAAGA(8/7)
427 GAAGA
MspA1I 148 CAGCTG C[AC]G^C[GT]G
381 CAGCGG
NaeI 48 GCCGGC GCC^GGC
75 GCCGGC
NgoMIV 48 GCCGGC G^CCGGC
75 GCCGGC
StuI 203 AGGCCT AGG^CCT
572 AGGCCT
StyI 29 CCAAGG C^C[AT][AT]GG
501 CCTTGG
TseI 150 GCTGC G^C[AT]GC
555 GCTGC
#########################################
# 3 OR MORE CUT ENZYMES ( 36 enzymes) #
#########################################
AciI BmyI BpmI BsaJI BsiYI
BslI BsmAI BsmFI Bsp1286I BssKI
BstNI Cac8I CfoI Cfr13I DpnII
EcoRII Fnu4HI HaeIII HapII HhaI
HinP1I HpaII ItaI MaeII MboI
MnlI MspI MvaI MwoI NdeII
NlaIII NlaIV Sau3AI Sau96I ScrFI
TspRI