Restriction map for
pFN21AE0216
(Linear) (Six-base) MAPSORT of: KIEE0216.seq Check: 2522 from: 1 to: 582
KIEE0216.orf
With 182 enzymes: SgfI *
April 24, 2009 13:11 ..
AflIII A'CryG_T
Cuts at: 0 233 582
Size: 233 349
AlwNI CAG_nnn'CTG
Cuts at: 0 467 582
Size: 467 115
ApaI G_GGCC'C
Cuts at: 0 192 582
Size: 192 390
AvaI C'yCGr_G
Cuts at: 0 403 582
Size: 403 179
BanI G'GyrC_C
Cuts at: 0 51 582
Size: 51 531
BanII G_rGCy'C
Cuts at: 0 192 530 582
Size: 192 338 52
Fragments arranged by size:
338 192 52
BbeI G_GCGC'C
Cuts at: 0 55 582
Size: 55 527
BglI GCCn_nnn'nGGC
Cuts at: 0 299 582
Size: 299 283
Bme1580I G_kGCm'C
Cuts at: 0 192 582
Size: 192 390
BmgBI CAC'GTC
Cuts at: 0 90 171 234 582
Size: 90 81 63 348
Fragments arranged by size:
348 90 81 63
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 222 582
Size: 222 360
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 37 79 444 582
Size: 37 42 365 138
Fragments arranged by size:
365 138 42 37
BsaI GGTCTCn'nnnn_
Cuts at: 0 502 582
Size: 502 80
BsaHI Gr'CG_yC
Cuts at: 0 52 582
Size: 52 530
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 79 109 582
Size: 79 30 473
Fragments arranged by size:
473 79 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 427 582
Size: 427 155
BseYI C'CCAG_C
Cuts at: 0 146 390 442 582
Size: 146 244 52 140
Fragments arranged by size:
244 146 140 52
BsiHKAI G_wGCw'C
Cuts at: 0 137 224 582
Size: 137 87 358
Fragments arranged by size:
358 137 87
BsmBI CGTCTCn'nnnn_
Cuts at: 0 264 582
Size: 264 318
Bsp1286I G_dGCh'C
Cuts at: 0 137 192 224 530 582
Size: 137 55 32 306 52
Fragments arranged by size:
306 137 55 52 32
BspHI T'CATG_A
Cuts at: 0 23 582
Size: 23 559
BsrFI r'CCGG_y
Cuts at: 0 48 75 582
Size: 48 27 507
Fragments arranged by size:
507 48 27
BtsI GCAGTG_nn'
Cuts at: 0 86 281 582
Size: 86 195 301
Fragments arranged by size:
301 195 86
EarI CTCTTCn'nnn_
Cuts at: 0 422 582
Size: 422 160
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 37 79 444 582
Size: 37 42 365 138
Fragments arranged by size:
365 138 42 37
EcoNI CCTnn'n_nnAGG
Cuts at: 0 163 582
Size: 163 419
EcoO109I rG'GnC_Cy
Cuts at: 0 188 189 582
Size: 188 1 393
Fragments arranged by size:
393 188 1
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 345 582
Size: 345 237
HaeII r_GCGC'y
Cuts at: 0 55 477 582
Size: 55 422 105
Fragments arranged by size:
422 105 55
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 79 111 242 274 582
Size: 79 32 131 32 308
Fragments arranged by size:
308 131 79 32 32
KasI G'GCGC_C
Cuts at: 0 51 582
Size: 51 531
MslI CAynn'nnrTG
Cuts at: 0 217 582
Size: 217 365
MspA1I CmG'CkG
Cuts at: 0 150 383 582
Size: 150 233 199
Fragments arranged by size:
233 199 150
NaeI GCC'GGC
Cuts at: 0 50 77 582
Size: 50 27 505
Fragments arranged by size:
505 50 27
NarI GG'CG_CC
Cuts at: 0 52 582
Size: 52 530
NgoMIV G'CCGG_C
Cuts at: 0 48 75 582
Size: 48 27 507
Fragments arranged by size:
507 48 27
NruI TCG'CGA
Cuts at: 0 398 582
Size: 398 184
NspI r_CATG'y
Cuts at: 0 48 582
Size: 48 534
PspOMI G'GGCC_C
Cuts at: 0 188 582
Size: 188 394
PvuII CAG'CTG
Cuts at: 0 150 582
Size: 150 432
SfoI GGC'GCC
Cuts at: 0 53 582
Size: 53 529
StuI AGG'CCT
Cuts at: 0 205 574 582
Size: 205 369 8
Fragments arranged by size:
369 205 8
StyI C'CwwG_G
Cuts at: 0 29 501 582
Size: 29 472 81
Fragments arranged by size:
472 81 29
TatI w'GTAC_w
Cuts at: 0 104 582
Size: 104 478
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 220 252 582
Size: 220 32 330
Fragments arranged by size:
330 220 32
Enzymes that do cut:
AflIII AlwNI ApaI AvaI BanI BanII BbeI BglI
Bme1580I BmgBI BplI BpmI BsaI BsaHI BsaXI BseRI
BseYI BsiHKAI BsmBI Bsp1286I BspHI BsrFI BtsI EarI
Eco57MI EcoNI EcoO109I FalI HaeII Hin4I KasI MslI
MspA1I NaeI NarI NgoMIV NruI NspI PspOMI PvuII
SfoI StuI StyI TatI TstI
Enzymes that do not cut:
AarI AatII AccI Acc65I AclI AcuI AfeI AflII
AgeI AhdI AleI AlfI AloI ApaLI ApoI AscI
AseI AsiSI AvrII BaeI BamHI BbsI BbvCI BcgI
BciVI BclI BfrBI BglII BlpI BmrI BmtI Bpu10I
BpuEI BsaAI BsaBI BsaWI BsgI BsiEI BsiWI BsmI
BspEI BspMI BsrBI BsrDI BsrGI BssHII BssSI BstAPI
BstBI BstEII BstXI BstYI BstZ17I Bsu36I BtgI BtgZI
ClaI CspCI DraI DraIII DrdI EaeI EagI EciI
EcoICRI EcoRI EcoRV FseI FspI FspAI HincII HindIII
HpaI KpnI MfeI MluI MmeI MscI NcoI NdeI
NheI NotI NsiI PacI PasI PciI PflMI PfoI
PmeI PmlI PpiI PpuMI PshAI PsiI PspXI PsrI
PstI PvuI RsrII SacI SacII SalI SanDI SapI
SbfI ScaI SexAI SfcI SfiI SgfI SgrAI SmaI
SmlI SnaBI SpeI SphI SrfI SspI SwaI TaqII
Tth111I XbaI XcmI XhoI XmaI XmnI ZraI