Restriction map for
pFN21AE9528
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# NO CUT ENZYMES ( 160 enzymes) #
##################################
AatII Acc65I AccII AccIII AciI
AclI AcyI AflII AgeI AhdI
Alw44I AlwI AocI Aor51HI ApaI
ApaLI AscI Asp700I Asp718I AspEI
AspHI AspI AviII AvrII BamHI
BanI BanII BbeI BbrPI BbsI
BbvCI BcgI BciVI BcnI BfaI
BfrI BglI BglII BlnI BlpI
BmyI BpmI Bpu1102I BpuAI BsaHI
BseAI BsgI BsiEI BsiHKAI BsiWI
BsmBI BsmFI Bsp1286I Bsp1407I BspDI
BspEI BspHI BspLU11I BsrBI BsrFI
BsrGI BssHII BssSI Bst1107I BstAPI
BstBI BstEII BstPI BstUI BstXI
BstYI BstZ17I Bsu36I CelII CfoI
Cfr10I ClaI CpoI DraIII DsaI
EagI Eam1105I EarI EclXI Eco47III
Eco52I Eco81I EcoNI EcoO65I EcoT22I
FauI FseI FspI HaeII HgaI
HhaI Hin1I HinP1I HindIII HpaI
KasI KpnI Ksp632I KspI MaeI
MfeI MflI MluI MroI MunI
MvnI NaeI NarI NciI NcoI
NdeI NgoMIV NheI NotI NruI
NsiI NspV PacI PflFI PinAI
PmaCI PmeI PmlI PshAI Psp1406I
PvuI RcaI RsrII SacI SacII
SalI SapI SbfI ScaI SexAI
SfiI SfoI SfuI SgrAI SmaI
SnaBI SpeI SphI Sse8387I SspBI
SspI StuI SwaI Tsp45I Tth111I
XbaI XhoII XmaCI XmaI XmnI
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# ONE CUT ENZYMES ( 47 enzymes) #
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AccI 295 GTCTAC GT^[AC][GT]AC
AcsI 3 GAATTC [GA]^AATT[CT]
AflIII 587 ACGTGT A^C[GA][CT]GT
AlwNI 70 CAGCTTCTG CAG[ACGT][ACGT][ACGT]^CTG
ApoI 3 GAATTC [GA]^AATT[CT]
AseI 303 ATTAAT AT^TAAT
AsnI 303 ATTAAT AT^TAAT
BalI 110 TGGCCA TGG^CCA
BsaAI 586 TACGTG [CT]AC^GT[GA]
BsaWI 1019 TCCGGT [AT]^CCGG[AT]
BseRI 640 CTCCTC GAGGAG(10/8)
BsmI 63 GAATGC GAATGC(1/-1)
BspMI 883 ACCTGC ACCTGC(4/8)
DraI 1114 TTTAAA TTT^AAA
DraII 488 GGGTCCT [GA]G^G[ACGT]CC[CT]
DrdI 310 GACCACTGGGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EaeI 110 TGGCCA [CT]^GGCC[GA]
EcoO109I 488 GGGTCCT [GA]G^G[ACGT]CC[CT]
EcoRI 3 GAATTC G^AATTC
EcoRV 930 GATATC GAT^ATC
EcoT14I 107 CCTTGG C^C[AT][AT]GG
HapII 1020 CCGG C^CGG
HincII 943 GTCAAC GT[CT]^[GA]AC
HindII 943 GTCAAC GT[CT]^[GA]AC
HpaII 1020 CCGG C^CGG
HphI 325 GGTGA GGTGA(8/7)
MaeII 587 ACGT A^CGT
MluNI 110 TGGCCA TGG^CCA
MscI 110 TGGCCA TGG^CCA
MspA1I 418 CAGCTG C[AC]G^C[GT]G
MspI 1020 CCGG C^CGG
MwoI 136 GCTTATGGAGC GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC
NspI 459 ACATGC [GA]CATG^[CT]
PaeR7I 434 CTCGAG C^TCGAG
PflMI 69 CCAGCTTCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PleI 613 GACTC GAGTC(4/5)
PpuMI 488 GGGTCCT [GA]G^G[AT]CC[CT]
PshBI 303 ATTAAT AT^TAAT
PstI 652 CTGCAG CTGCA^G
PvuII 418 CAGCTG CAG^CTG
SfcI 652 CTGCAG C^T[GA][CT]AG
SmlI 434 CTCGAG C^T[CT][GA]AG
StyI 107 CCTTGG C^C[AT][AT]GG
TaqI 435 TCGA T^CGA
TthHB8I 435 TCGA T^CGA
Van91I 69 CCAGCTTCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XhoI 434 CTCGAG C^TCGAG
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# TWO CUT ENZYMES ( 20 enzymes) #
###################################
AfaI 573 GTAC GT^AC
756 GTAC
AvaI 434 CTCGAG C^[CT]CG[GA]G
1025 CTCGGG
BbvI 651 GCTGC GCAGC(8/12)
792 GCTGC
BsaI 444 GAGACC GGTCTC(1/5)
1022 GGTCTC
BsaJI 20 CCCAGG C^C[ACGT][ACGT]GG
107 CCTTGG
BsoBI 434 CTCGAG C^[CT]CG[GA]G
1025 CTCGGG
BssKI 21 CCAGG ^CC[ACGT]GG
174 CCTGG
BstF5I 228 CATCC GGATG(2/0)
1029 GGATG
BstNI 21 CCAGG CC^[AT]GG
174 CCTGG
EcoRII 21 CCAGG ^CC[AT]GG
174 CCTGG
Fnu4HI 651 GCTGC GC^[ACGT]GC
792 GCTGC
FokI 228 CATCC GGATG(9/13)
1029 GGATG
HaeIII 111 GGCC GG^CC
177 GGCC
ItaI 651 GCTGC GC^[ACGT]GC
792 GCTGC
MaeIII 220 GTTAC ^GT[ACGT]AC
559 GTAAC
MvaI 21 CCAGG CC^[AT]GG
174 CCTGG
RsaI 573 GTAC GT^AC
756 GTAC
ScrFI 21 CCAGG CC^[ACGT]GG
174 CCTGG
SfaNI 568 GATGC GCATC(5/9)
1062 GATGC
TseI 651 GCTGC G^C[AT]GC
792 GCTGC
#########################################
# 3 OR MORE CUT ENZYMES ( 31 enzymes) #
#########################################
AluI AvaII BclI BsaBI BsiYI
BslI BsmAI BsrDI BsrI Cac8I
Cfr13I DdeI DpnII FbaI HinfI
MamI MboI MboII MnlI MseI
MslI NdeII NlaIII NlaIV Sau3AI
Sau96I TfiI Tru9I Tsp509I TspRI
XcmI