Restriction map for
pFN21AE6715
##################################
# NO CUT ENZYMES ( 92 enzymes) #
##################################
Acc65I AccIII AclI AcsI AflII
AgeI Alw44I ApaI ApaLI ApoI
AscI AseI AsnI Asp700I Asp718I
AvrII BamHI BbsI BcgI BciVI
BclI BfrI BglII BlnI BpuAI
BsaBI BseAI BsiWI BsmI BspDI
BspEI BspHI BspLU11I BsrDI BssHII
Bst1107I BstBI BstEII BstPI BstZ17I
ClaI CpoI DraI DraIII DrdI
EarI EcoO65I EcoRI EcoRV EcoT22I
FbaI HincII HindII HindIII HpaI
KpnI Ksp632I MamI MfeI MluI
MroI MunI NdeI NheI NotI
NruI NsiI NspV PacI PflMI
PinAI PmeI PshBI Psp1406I PvuI
RcaI RsrII SalI SapI SbfI
ScaI SexAI SfuI SgrAI SnaBI
SpeI Sse8387I SspI StuI SwaI
Van91I XmnI
###################################
# ONE CUT ENZYMES ( 44 enzymes) #
###################################
AatII 483 GACGTC GACGT^C
AccI 154 GTCTAC GT^[AC][GT]AC
AflIII 176 ACACGT A^C[GA][CT]GT
AhdI 31 GACGGAGGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AocI 477 CCTGAGG CC^T[ACGT]AGG
Aor51HI 911 AGCGCT AGC^GCT
AspEI 31 GACGGAGGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AspI 1725 GACGGAGTC GAC[ACGT]^[ACGT][ACGT]GTC
BalI 701 TGGCCA TGG^CCA
BbrPI 1746 CACGTG CAC^GTG
BfaI 2122 CTAG C^TAG
BsaWI 1832 ACCGGA [AT]^CCGG[AT]
BsiEI 566 CGGCCG CG[GA][CT]^CG
BsmBI 1864 GAGACG CGTCTC(1/5)
Bsp1407I 245 TGTACA T^GTACA
BsrGI 245 TGTACA T^GTACA
BstAPI 1956 GCAGAGACTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
Bsu36I 477 CCTGAGG CC^T[ACGT]AGG
EagI 566 CGGCCG C^GGCCG
Eam1105I 31 GACGGAGGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EclXI 566 CGGCCG C^GGCCG
Eco47III 911 AGCGCT AGC^GCT
Eco52I 566 CGGCCG C^GGCCG
Eco81I 477 CCTGAGG CC^T[ACGT]AGG
FseI 1997 GGCCGGCC GGCCGG^CC
MaeI 2122 CTAG C^TAG
MluNI 701 TGGCCA TGG^CCA
MscI 701 TGGCCA TGG^CCA
NspI 410 GCATGC [GA]CATG^[CT]
PflFI 1725 GACGGAGTC GAC[ACGT]^[ACGT][ACGT]GTC
PmaCI 1746 CACGTG CAC^GTG
PmlI 1746 CACGTG CAC^GTG
PshAI 1961 GACTGCGGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
PvuII 1587 CAGCTG CAG^CTG
SfiI 94 GGCCCCAAGGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
SmaI 1696 CCCGGG CCC^GGG
SphI 410 GCATGC GCATG^C
SspBI 245 TGTACA T^GTACA
TfiI 269 GATTC G^A[AT]TC
Tsp509I 22 AATT ^AATT
Tth111I 1725 GACGGAGTC GAC[ACGT]^[ACGT][ACGT]GTC
XbaI 2121 TCTAGA T^CTAGA
XmaCI 1696 CCCGGG C^CCGGG
XmaI 1696 CCCGGG C^CCGGG
###################################
# TWO CUT ENZYMES ( 22 enzymes) #
###################################
AviII 500 TGCGCA TGC^GCA
674 TGCGCA
BbeI 956 GGCGCC GGCGC^C
1900 GGCGCC
BbvCI 805 GCTGAGG CCTCAGC(-5/-2)
1854 GCTGAGG
BsaI 364 GAGACC GGTCTC(1/5)
856 GAGACC
BspMI 14 GCAGGT ACCTGC(4/8)
1509 GCAGGT
BstXI 788 CCATCAGCCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1793 CCAGGCTGCTGG
BstYI 614 AGATCC [GA]^GATC[CT]
1073 AGATCC
FspI 500 TGCGCA TGC^GCA
674 TGCGCA
KasI 956 GGCGCC G^GCGCC
1900 GGCGCC
MboII 630 GAAGA GAAGA(8/7)
876 GAAGA
MflI 614 AGATCC [GA]^GATC[CT]
1073 AGATCC
MseI 20 TTAA T^TAA
2231 TTAA
NaeI 556 GCCGGC GCC^GGC
1998 GCCGGC
NarI 956 GGCGCC GG^CGCC
1900 GGCGCC
NcoI 78 CCATGG C^CATGG
960 CCATGG
NgoMIV 556 GCCGGC G^CCGGC
1998 GCCGGC
PaeR7I 1503 CTCGAG C^TCGAG
1933 CTCGAG
SacI 723 GAGCTC GAGCT^C
2149 GAGCTC
SfoI 956 GGCGCC GGC^GCC
1900 GGCGCC
Tru9I 20 TTAA T^TAA
2231 TTAA
XhoI 1503 CTCGAG C^TCGAG
1933 CTCGAG
XhoII 614 AGATCC [GA]^GATC[CT]
1073 AGATCC
#########################################
# 3 OR MORE CUT ENZYMES ( 100 enzymes) #
#########################################
AccII AciI AcyI AfaI AluI
AlwI AlwNI AspHI AvaI AvaII
BanI BanII BbvI BcnI BglI
BlpI BmyI BpmI Bpu1102I BsaAI
BsaHI BsaJI BseRI BsgI BsiHKAI
BsiYI BslI BsmAI BsmFI BsoBI
Bsp1286I BsrBI BsrFI BsrI BssKI
BssSI BstF5I BstNI BstUI Cac8I
CelII CfoI Cfr10I Cfr13I DdeI
DpnII DraII DsaI EaeI EcoNI
EcoO109I EcoRII EcoT14I FauI Fnu4HI
FokI HaeII HaeIII HapII HgaI
HhaI Hin1I HinP1I HinfI HpaII
HphI ItaI KspI MaeII MaeIII
MboI MnlI MslI MspA1I MspI
MvaI MvnI MwoI NciI NdeII
NlaIII NlaIV PleI PpuMI PstI
RsaI SacII Sau3AI Sau96I ScrFI
SfaNI SfcI SmlI StyI TaqI
TseI Tsp45I TspRI TthHB8I XcmI