################################## # NO CUT ENZYMES ( 149 enzymes) # ################################## AatII AccIII AclI AcsI AflII AflIII AgeI AhdI Alw44I AlwI AlwNI AocI Aor51HI ApaLI ApoI AscI AseI AsnI Asp700I AspEI AspI AvrII BalI BamHI BbrPI BbsI BbvCI BcgI BciVI BclI BfaI BfrI BglII BlnI BlpI Bpu1102I BpuAI BsaAI BsaBI BsaI BsaWI BseAI BsiWI BsmAI BsmBI BsmI Bsp1407I BspDI BspEI BspHI BspLU11I BsrBI BsrDI BsrGI BssSI Bst1107I BstAPI BstBI BstEII BstPI BstYI BstZ17I Bsu36I CelII ClaI DpnII DraI DraIII DrdI Eam1105I EarI Eco47III Eco81I EcoNI EcoO65I EcoRI EcoRV EcoT14I EcoT22I FbaI FseI HincII HindII HindIII HpaI Ksp632I MaeI MamI MboI MboII MfeI MflI MluI MluNI MroI MscI MseI MunI NcoI NdeI NdeII NheI NruI NsiI NspV PacI PaeR7I PflFI PflMI PinAI PleI PmaCI PmeI PmlI PpuMI PshAI PshBI Psp1406I PvuI RcaI SacI SalI SapI Sau3AI SbfI ScaI SexAI SfiI SfuI SmaI SmlI SnaBI SpeI SphI Sse8387I SspBI SspI StyI SwaI Tru9I Tsp509I Tth111I Van91I XbaI XhoI XhoII XmaCI XmaI XmnI ################################### # ONE CUT ENZYMES ( 36 enzymes) # ################################### ApaI 579 GGGCCC GGGCC^C AspHI 148 GTGCTC G[AT]GC[AT]^C AvaII 96 GGACC G^G[AT]CC AviII 263 TGCGCA TGC^GCA BanII 579 GGGCCC G[GA]GC[CT]^C BglI 52 GCCCCGAGGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC BsgI 498 CTGCAC GTGCAG(16/14) BsiHKAI 148 GTGCTC G[AT]GC[AT]^C BsmFI 508 GTCCC GGGAC(10/14) BsrI 114 ACTGG ACTGG(1/-1) BssHII 196 GCGCGC G^CGCGC BstF5I 486 CATCC GGATG(2/0) BstXI 485 CCATCCCCGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG CpoI 95 CGGACCG CG^G[AT]CCG DdeI 151 CTCAG C^T[ACGT]AG DraII 579 GGGCCCC [GA]G^G[ACGT]CC[CT] EcoO109I 579 GGGCCCC [GA]G^G[ACGT]CC[CT] FokI 486 CATCC GGATG(9/13) FspI 263 TGCGCA TGC^GCA HinfI 290 GAATC G^A[ACGT]TC HphI 482 TCACC GGTGA(8/7) MaeIII 481 GTCAC ^GT[ACGT]AC MslI 486 CATCCCCGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG NlaIII 411 CATG CATG^ NotI 78 GCGGCCGC GC^GGCCGC NspI 410 ACATGC [GA]CATG^[CT] PstI 172 CTGCAG CTGCA^G RsrII 95 CGGACCG CG^G[AT]CCG SgrAI 447 CGCCGGCG C[GA]^CCGG[CT]G StuI 473 AGGCCT AGG^CCT TaqI 551 TCGA T^CGA TfiI 290 GAATC G^A[AT]TC Tsp45I 481 GTCAC ^GT[GC]AC TspRI 153 CAGTG CA[GC]TG[ACGT][ACGT]^ TthHB8I 551 TCGA T^CGA XcmI 566 CCAAGCTCCTGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG ################################### # TWO CUT ENZYMES ( 16 enzymes) # ################################### Acc65I 311 GGTACC G^GTACC 320 GGTACC AccI 406 GTCTAC GT^[AC][GT]AC 463 GTCTAC Asp718I 311 GGTACC G^GTACC 320 GGTACC AvaI 54 CCCGAG C^[CT]CG[GA]G 511 CCCGAG BmyI 148 GTGCTC G[AGT]GC[ACT]^C 579 GGGCCC BpmI 543 CTCCAG CTGGAG(16/14) 115 CTGGAG BseRI 106 GAGGAG GAGGAG(10/8) 376 GAGGAG BsoBI 54 CCCGAG C^[CT]CG[GA]G 511 CCCGAG Bsp1286I 148 GTGCTC G[AGT]GC[ACT]^C 579 GGGCCC KpnI 311 GGTACC GGTAC^C 320 GGTACC KspI 44 CCGCGG CCGC^GG 82 CCGCGG MaeII 383 ACGT A^CGT 479 ACGT PvuII 121 CAGCTG CAG^CTG 175 CAGCTG SacII 44 CCGCGG CCGC^GG 82 CCGCGG SfaNI 536 GCATC GCATC(5/9) 548 GCATC SfcI 172 CTGCAG C^T[GA][CT]AG 285 CTACAG ######################################### # 3 OR MORE CUT ENZYMES ( 57 enzymes) # ######################################### AccII AciI AcyI AfaI AluI BanI BbeI BbvI BcnI BsaHI BsaJI BsiEI BsiYI BslI BspMI BsrFI BssKI BstNI BstUI Cac8I CfoI Cfr10I Cfr13I DsaI EaeI EagI EclXI Eco52I EcoRII FauI Fnu4HI HaeII HaeIII HapII HgaI HhaI Hin1I HinP1I HpaII ItaI KasI MnlI MspA1I MspI MvaI MvnI MwoI NaeI NarI NciI NgoMIV NlaIV RsaI Sau96I ScrFI SfoI TseI