################################## # NO CUT ENZYMES ( 137 enzymes) # ################################## AatII Acc65I AccIII AclI AcyI AflII AgeI AhdI Alw44I AocI Aor51HI ApaLI AscI Asp700I Asp718I AspEI AviII AvrII BalI BamHI BbeI BbsI BbvCI BcgI BclI BfaI BfrI BglI BlnI BlpI Bpu1102I BpuAI BsaHI BsaI BsaWI BseAI BseRI BsiWI BsmI BspDI BspEI BspMI BsrBI BsrDI BssHII Bst1107I BstAPI BstBI BstEII BstPI BstXI BstZ17I Bsu36I CelII CfoI ClaI CpoI DraIII DrdI EagI Eam1105I EarI EclXI Eco47III Eco52I Eco81I EcoNI EcoO65I EcoRI EcoRV EcoT22I FbaI FseI FspI HaeII HgaI HhaI Hin1I HinP1I HincII HindII HpaI KasI KpnI Ksp632I KspI MaeI MfeI MluI MluNI MroI MscI MslI MunI NaeI NarI NgoMIV NheI NotI NruI NsiI NspV PacI PaeR7I PflMI PinAI PmeI PpuMI PshAI Psp1406I PstI PvuI RsrII SacI SacII SalI SapI SbfI ScaI SexAI SfiI SfoI SfuI SgrAI SmaI SnaBI SpeI SphI Sse8387I StuI SwaI Van91I XbaI XhoI XmaCI XmaI XmnI ################################### # ONE CUT ENZYMES ( 53 enzymes) # ################################### AccI 1172 GTCTAC GT^[AC][GT]AC AccII 318 CGCG CG^CG AciI 312 CCGC CCGC(-3/-1) AlwNI 655 CAGTTTCTG CAG[ACGT][ACGT][ACGT]^CTG ApaI 1203 GGGCCC GGGCC^C AseI 147 ATTAAT AT^TAAT AsnI 147 ATTAAT AT^TAAT AspHI 113 GTGCTC G[AT]GC[AT]^C AspI 1166 GACCATGTC GAC[ACGT]^[ACGT][ACGT]GTC AvaI 1295 CCCGAG C^[CT]CG[GA]G AvaII 1165 GGACC G^G[AT]CC BanII 1203 GGGCCC G[GA]GC[CT]^C BbrPI 7 CACGTG CAC^GTG BciVI 760 GGATAC GTATCC(6/5) BcnI 1269 CCGGG CC^[GC]GG BglII 103 AGATCT A^GATCT BsaBI 1372 GATATTCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC BsgI 690 CTGCAC GTGCAG(16/14) BsiEI 1266 CGACCG CG[GA][CT]^CG BsiHKAI 113 GTGCTC G[AT]GC[AT]^C BsiYI 1215 CCCTGTCCAGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG BslI 1215 CCCTGTCCAGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG BsmBI 256 GAGACG CGTCTC(1/5) BsmFI 1164 GGGAC GGGAC(10/14) BsoBI 1295 CCCGAG C^[CT]CG[GA]G BspHI 423 TCATGA T^CATGA BspLU11I 550 ACATGT A^CATGT BsrFI 846 ACCGGC [GA]^CCGG[CT] BssSI 908 CACGAG CACGAG(-5/-1) BstUI 318 CGCG CG^CG Cac8I 1300 GCCTGC GC[ACGT]^[ACGT]GC Cfr10I 846 ACCGGC [GA]^CCGG[CT] DraI 193 TTTAAA TTT^AAA EaeI 848 CGGCCA [CT]^GGCC[GA] FauI 311 CCCGC CCCGC(4/6) HindIII 570 AAGCTT A^AGCTT HphI 124 TCACC GGTGA(8/7) MamI 1372 GATATTCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC MspA1I 1004 CAGCTG C[AC]G^C[GT]G MvnI 318 CGCG CG^CG NciI 1269 CCGGG CC^[GC]GG NdeI 932 CATATG CA^TATG PflFI 1166 GACCATGTC GAC[ACGT]^[ACGT][ACGT]GTC PmaCI 7 CACGTG CAC^GTG PmlI 7 CACGTG CAC^GTG PshBI 147 ATTAAT AT^TAAT PvuII 1004 CAGCTG CAG^CTG RcaI 423 TCATGA T^CATGA SfcI 434 CTACAG C^T[GA][CT]AG SmlI 523 CTCAAG C^T[CT][GA]AG SspI 1330 AATATT AAT^ATT Tth111I 1166 GACCATGTC GAC[ACGT]^[ACGT][ACGT]GTC XcmI 851 CCAGGGTAGCTCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG ################################### # TWO CUT ENZYMES ( 21 enzymes) # ################################### AflIII 550 ACATGT A^C[GA][CT]GT 721 ACGTGT BanI 412 GGTGCC G^G[CT][GA]CC 618 GGCACC BmyI 113 GTGCTC G[AGT]GC[ACT]^C 1203 GGGCCC BsaAI 7 CACGTG [CT]AC^GT[GA] 720 TACGTG Bsp1286I 113 GTGCTC G[AGT]GC[ACT]^C 1203 GGGCCC Bsp1407I 543 TGTACA T^GTACA 779 TGTACA BsrGI 543 TGTACA T^GTACA 779 TGTACA BstYI 103 AGATCT [GA]^GATC[CT] 519 GGATCT DsaI 263 CCATGG C^C[GA][CT]GG 536 CCATGG EcoT14I 263 CCATGG C^C[AT][AT]GG 536 CCATGG HapII 847 CCGG C^CGG 1269 CCGG HpaII 847 CCGG C^CGG 1269 CCGG MaeII 8 ACGT A^CGT 721 ACGT MflI 103 AGATCT [GA]^GATC[CT] 519 GGATCT MspI 847 CCGG C^CGG 1269 CCGG NcoI 263 CCATGG C^CATGG 536 CCATGG NspI 217 ACATGC [GA]CATG^[CT] 550 ACATGT SfaNI 172 GATGC GCATC(5/9) 838 GATGC SspBI 543 TGTACA T^GTACA 779 TGTACA StyI 263 CCATGG C^C[AT][AT]GG 536 CCATGG XhoII 103 AGATCT [GA]^GATC[CT] 519 GGATCT ######################################### # 3 OR MORE CUT ENZYMES ( 47 enzymes) # ######################################### AcsI AfaI AluI AlwI ApoI BbvI BpmI BsaJI BsmAI BsrI BssKI BstF5I BstNI Cfr13I DdeI DpnII DraII EcoO109I EcoRII Fnu4HI FokI HaeIII HinfI ItaI MaeIII MboI MboII MnlI MseI MvaI MwoI NdeII NlaIII NlaIV PleI RsaI Sau3AI Sau96I ScrFI TaqI TfiI Tru9I TseI Tsp45I Tsp509I TspRI TthHB8I