(Circular) (Six-base) MAPSORT of: pFN21AE5520.seq Check: 1941 from: 1 to: 6097 pFN21AE5520 6097 bp With 182 enzymes: SgfI * January 30, 2014 17:21 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3907 278 Size: 53 83 186 936 2371 2468 Fragments arranged by size: 2468 2371 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3178 3440 1937 Size: 1241 262 4594 Fragments arranged by size: 4594 1241 262 Acc65I G'GTAC_C Cuts at: 3418 3418 Size: 6097 AclI AA'CG_TT Cuts at: 1563 4224 4597 1563 Size: 2661 373 3063 Fragments arranged by size: 3063 2661 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 4160 5544 5812 1632 Size: 2528 1384 268 1917 Fragments arranged by size: 2528 1917 1384 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5900 AflIII A'CryG_T Cuts at: 1203 2555 2726 4996 5973 1203 Size: 1352 171 2270 977 1327 Fragments arranged by size: 2270 1352 1327 977 171 AgeI A'CCGG_T Cuts at: 4928 4928 Size: 6097 AhdI GACnn_n'nnGTC Cuts at: 4826 4826 Size: 6097 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 6097 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 6097 AlwNI CAG_nnn'CTG Cuts at: 2665 3840 5412 2665 Size: 1175 1572 3350 Fragments arranged by size: 3350 1572 1175 ApaI G_GGCC'C Cuts at: 3212 3212 Size: 6097 ApaLI G'TGCA_C Cuts at: 4154 5310 4154 Size: 1156 4941 ApoI r'AATT_y Cuts at: 1493 2460 2533 3016 3360 3406 3632 3784 5893 Size: 967 73 483 344 46 226 152 2109 Cuts at: 5893 1493 Size: 1697 Fragments arranged by size: 2109 1697 967 483 344 226 152 73 46 AseI AT'TA_AT Cuts at: 160 1032 2153 4651 160 Size: 872 1121 2498 1606 Fragments arranged by size: 2498 1606 1121 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6097 AvaI C'yCGr_G Cuts at: 1870 1943 3300 3422 1870 Size: 73 1357 122 4545 Fragments arranged by size: 4545 1357 122 73 BamHI G'GATC_C Cuts at: 3427 3794 3427 Size: 367 5730 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2417 2623 3418 4873 618 Size: 325 217 627 630 206 795 1455 1842 Fragments arranged by size: 1842 1455 795 630 627 325 217 206 BanII G_rGCy'C Cuts at: 1391 3212 3416 1391 Size: 1821 204 4072 Fragments arranged by size: 4072 1821 204 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 928 Size: 971 5126 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 4288 4322 1903 Size: 34 2351 34 3678 Fragments arranged by size: 3678 2351 34 34 BciVI GTATCCnnnnn_n' Cuts at: 2759 3989 5199 2759 Size: 1230 1210 3657 Fragments arranged by size: 3657 1230 1210 BclI T'GATC_A Cuts at: 1548 1548 Size: 6097 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 4708 136 Size: 107 122 71 631 605 3036 1525 Fragments arranged by size: 3036 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 2108 6092 2108 Size: 3984 2113 BlpI GC'TnA_GC Cuts at: 3514 3514 Size: 6097 Bme1580I G_kGCm'C Cuts at: 1790 3212 4158 5314 1790 Size: 1422 946 1156 2573 Fragments arranged by size: 2573 1422 1156 946 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 6097 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2653 2787 3355 Size: 416 53 83 399 24 1236 134 568 Cuts at: 3355 3828 4786 442 Size: 473 958 1753 Fragments arranged by size: 1753 1236 958 568 473 416 399 134 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6097 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2193 2771 3020 3113 4757 1239 Size: 954 578 249 93 1644 2579 Fragments arranged by size: 2579 1644 954 578 249 93 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2513 4222 5087 5385 2513 Size: 1709 865 298 3225 Fragments arranged by size: 3225 1709 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 4760 882 Size: 617 3261 2219 Fragments arranged by size: 3261 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 2014 2727 3888 493 Size: 711 810 713 1161 2702 Fragments arranged by size: 2702 1161 810 713 711 BsaBI GATnn'nnATC Cuts at: 3381 6091 3381 Size: 2710 3387 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 3904 4286 4921 275 Size: 53 83 186 936 2371 382 635 1451 Fragments arranged by size: 2371 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 4530 4928 5202 5349 5760 4530 Size: 398 274 147 411 4867 Fragments arranged by size: 4867 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 5872 Fragments arranged by size: 5872 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 1186 Size: 6097 BseYI C'CCAG_C Cuts at: 5300 5796 5300 Size: 496 5601 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2680 1163 Size: 573 944 4580 Fragments arranged by size: 4580 944 573 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3274 3547 4308 5336 1540 Size: 275 186 1273 273 761 1028 2301 Fragments arranged by size: 2301 1273 1028 761 275 273 186 BsiHKAI G_wGCw'C Cuts at: 2122 4158 4243 5314 2122 Size: 2036 85 1071 2905 Fragments arranged by size: 2905 2036 1071 85 BsmI GAATG_Cn' Cuts at: 3608 3701 3608 Size: 93 6004 BsmBI CGTCTCn'nnnn_ Cuts at: 2255 2255 Size: 6097 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2122 3212 3416 4158 4243 5314 1391 Size: 399 332 1090 204 742 85 1071 2174 Fragments arranged by size: 2174 1090 1071 742 399 332 204 85 BspEI T'CCGG_A Cuts at: 5760 5760 Size: 6097 BspHI T'CATG_A Cuts at: 2428 3986 5716 2428 Size: 1558 1730 2809 Fragments arranged by size: 2809 1730 1558 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 3452 844 Size: 2608 3489 BsrBI CCG'CTC Cuts at: 1394 1823 3984 1394 Size: 429 2161 3507 Fragments arranged by size: 3507 2161 429 BsrDI GCAATG_nn' Cuts at: 65 1248 4592 4766 65 Size: 1183 3344 174 1396 Fragments arranged by size: 3344 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2851 4741 4928 1700 Size: 110 1041 1890 187 2869 Fragments arranged by size: 2869 1890 1041 187 110 BsrGI T'GTAC_A Cuts at: 96 2548 2784 96 Size: 2452 236 3409 Fragments arranged by size: 3409 2452 236 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6097 BssSI C'ACGA_G Cuts at: 2913 4157 5169 2913 Size: 1244 1012 3841 Fragments arranged by size: 3841 1244 1012 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 6097 BstBI TT'CG_AA Cuts at: 4901 4901 Size: 6097 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 6097 BstYI r'GATC_y Cuts at: 1972 2108 2524 3427 3794 4179 4196 5648 5734 Size: 136 416 903 367 385 17 1452 86 Cuts at: 5734 5746 6092 1972 Size: 12 346 1977 Fragments arranged by size: 1977 1452 903 416 385 367 346 136 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2004 2268 2541 513 Size: 552 939 264 273 4069 Fragments arranged by size: 4069 939 552 273 264 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5358 Fragments arranged by size: 5358 683 56 BtsI GCAGTG_nn' Cuts at: 3613 4407 4427 3613 Size: 794 20 5283 Fragments arranged by size: 5283 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6097 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 6062 DraI TTT'AAA Cuts at: 2200 3401 2200 Size: 1201 4896 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 5104 809 Size: 507 3788 1802 Fragments arranged by size: 3788 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2853 3544 4433 Size: 54 1425 50 275 18 1023 691 889 Cuts at: 4433 6062 8 Size: 1629 43 Fragments arranged by size: 1629 1425 1023 889 691 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3544 1537 Size: 275 1732 4090 Fragments arranged by size: 4090 1732 275 EarI CTCTTCn'nnn_ Cuts at: 1353 4027 1353 Size: 2674 3423 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4684 5058 5204 387 Size: 1064 138 3095 374 146 1280 Fragments arranged by size: 3095 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2193 2771 3020 3113 4160 4757 5544 Size: 393 561 578 249 93 1047 597 787 Cuts at: 5544 5812 1239 Size: 268 1524 Fragments arranged by size: 1524 1047 787 597 578 561 393 268 249 93 EcoO109I rG'GnC_Cy Cuts at: 2204 3208 3209 2204 Size: 1004 1 5092 Fragments arranged by size: 5092 1004 1 EcoRI G'AATT_C Cuts at: 3406 3784 3406 Size: 378 5719 FspI TGC'GCA Cuts at: 4603 4603 Size: 6097 HaeII r_GCGC'y Cuts at: 1678 5244 1678 Size: 3566 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2271 2303 3281 3313 4738 Size: 32 135 32 699 32 978 32 1425 Cuts at: 4738 4770 4812 4844 1373 Size: 32 42 32 2626 Fragments arranged by size: 2626 1425 978 699 135 42 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3441 3687 669 Size: 988 281 1503 246 3079 Fragments arranged by size: 3079 1503 988 281 246 HindIII A'AGCT_T Cuts at: 748 2575 748 Size: 1827 4270 HpaI GTT'AAC Cuts at: 3687 3687 Size: 6097 KpnI G_GTAC'C Cuts at: 3422 3422 Size: 6097 MfeI C'AATT_G Cuts at: 3696 3696 Size: 6097 MluI A'CGCG_T Cuts at: 5973 5973 Size: 6097 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2352 5211 5395 1305 Size: 1047 2859 184 2007 Fragments arranged by size: 2859 2007 1047 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 4275 Fragments arranged by size: 4275 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 4055 4414 4573 518 Size: 648 2889 359 159 2042 Fragments arranged by size: 2889 2042 648 359 159 MspA1I CmG'CkG Cuts at: 1606 3011 3513 4190 5338 5583 1606 Size: 1405 502 677 1148 245 2120 Fragments arranged by size: 2120 1405 1148 677 502 245 NcoI C'CATG_G Cuts at: 513 1065 2004 2268 2541 513 Size: 552 939 264 273 4069 Fragments arranged by size: 4069 939 552 273 264 NdeI CA'TA_TG Cuts at: 387 2938 387 Size: 2551 3546 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6097 NotI GC'GGCC_GC Cuts at: 3544 3544 Size: 6097 NspI r_CATG'y Cuts at: 1132 1229 2226 2559 3455 5000 1132 Size: 97 997 333 896 1545 2229 Fragments arranged by size: 2229 1545 997 896 333 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 6097 PciI A'CATG_T Cuts at: 2555 4996 2555 Size: 2441 3656 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 6097 PmeI GTTT'AAAC Cuts at: 3401 3401 Size: 6097 PmlI CAC'GTG Cuts at: 2014 2014 Size: 6097 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4166 4198 5679 5711 4166 Size: 32 1481 32 4552 Fragments arranged by size: 4552 1481 32 32 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 6097 PsiI TTA'TAA Cuts at: 3667 3667 Size: 6097 PspOMI G'GGCC_C Cuts at: 3208 3208 Size: 6097 PstI C_TGCA'G Cuts at: 830 3449 830 Size: 2619 3478 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6097 PvuII CAG'CTG Cuts at: 3011 3011 Size: 6097 SalI G'TCGA_C Cuts at: 1936 3439 1936 Size: 1503 4594 SbfI CC_TGCA'GG Cuts at: 3449 3449 Size: 6097 ScaI AGT'ACT Cuts at: 4345 6057 4345 Size: 1712 4385 SfcI C'TryA_G Cuts at: 826 1045 2439 3445 4580 5261 5452 826 Size: 219 1394 1006 1135 681 191 1471 Fragments arranged by size: 1471 1394 1135 1006 681 219 191 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6097 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6097 SmaI CCC'GGG Cuts at: 1872 3424 1872 Size: 1552 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2528 4201 5102 5364 820 Size: 197 926 585 1673 901 262 1553 Fragments arranged by size: 1673 1553 926 901 585 262 197 SnaBI TAC'GTA Cuts at: 493 3888 493 Size: 3395 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 6097 SphI G_CATG'C Cuts at: 1132 3455 1132 Size: 2323 3774 SspI AAT'ATT Cuts at: 5 52 3337 4021 5 Size: 47 3285 684 2081 Fragments arranged by size: 3285 2081 684 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 6097 StyI C'CwwG_G Cuts at: 513 1065 2004 2268 2541 3536 513 Size: 552 939 264 273 995 3074 Fragments arranged by size: 3074 995 939 552 273 264 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 4124 4141 4294 4479 4924 325 Size: 1255 2544 17 153 185 445 1498 Fragments arranged by size: 2544 1498 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2548 2784 4343 Size: 274 80 33 51 1443 571 236 1559 Cuts at: 4343 6055 96 Size: 1712 138 Fragments arranged by size: 1712 1559 1443 571 274 236 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 6057 6089 6057 Size: 32 6065 Tth111I GACn'n_nGTC Cuts at: 1316 3174 1316 Size: 1858 4239 XbaI T'CTAG_A Cuts at: 3433 3433 Size: 6097 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2863 2863 Size: 6097 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 6097 XmaI C'CCGG_G Cuts at: 1870 3422 1870 Size: 1552 4545 XmnI GAAnn'nnTTC Cuts at: 4226 4226 Size: 6097 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3905 276 Size: 53 83 186 936 2371 2468 Fragments arranged by size: 2468 2371 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PmeI PmlI PpiI PshAI PsiI PspOMI PstI PvuI PvuII SalI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI AscI AvrII BaeI BbeI BbvCI BfrBI BplI Bpu10I BsiWI BstXI BstZ17I Bsu36I DraIII EcoICRI EcoNI EcoRV FalI FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PfoI PpuMI PspXI PsrI RsrII SacI SacII SanDI SapI SexAI SfiI SfoI SrfI SwaI