(Linear) (Six-base) MAPSORT of: KIEE5520.seq  Check: 4  from: 1  to: 1392



KIEE5520.orf

 With 182 enzymes: SgfI * 

                        January 29, 2014 12:04  ..

AccI GT'mk_AC

Cuts at:      0    1173    1392 
   Size:       1173     219
 
AflIII A'CryG_T

Cuts at:      0     550     721    1392 
   Size:        550     171     671

  Fragments arranged by size: 

                 671     550     171
 
AlwNI CAG_nnn'CTG

Cuts at:      0     660    1392 
   Size:        660     732
 
ApaI G_GGCC'C

Cuts at:      0    1207    1392 
   Size:       1207     185
 
ApoI r'AATT_y

Cuts at:      0     455     528    1011    1355    1392 
   Size:        455      73     483     344      37

  Fragments arranged by size: 

                 483     455     344      73      37
 
AseI AT'TA_AT

Cuts at:      0     148    1392 
   Size:        148    1244
 
AvaI C'yCGr_G

Cuts at:      0    1295    1392 
   Size:       1295      97
 
BanI G'GyrC_C

Cuts at:      0     412     618    1392 
   Size:        412     206     774

  Fragments arranged by size: 

                 774     412     206
 
BanII G_rGCy'C

Cuts at:      0    1207    1392 
   Size:       1207     185
 
BciVI GTATCCnnnnn_n'

Cuts at:      0     754    1392 
   Size:        754     638
 
BglII A'GATC_T

Cuts at:      0     103    1392 
   Size:        103    1289
 
Bme1580I G_kGCm'C

Cuts at:      0    1207    1392 
   Size:       1207     185
 
BmrI ACTGGGnnnn_n'

Cuts at:      0     648     782    1350    1392 
   Size:        648     134     568      42

  Fragments arranged by size: 

                 648     568     134      42
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     188     766    1015    1108    1392 
   Size:        188     578     249      93     284

  Fragments arranged by size: 

                 578     284     249     188      93
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     508    1392 
   Size:        508     884
 
BsaAI yAC'GTr

Cuts at:      0       9     722    1392 
   Size:          9     713     670

  Fragments arranged by size: 

                 713     670       9
 
BsaBI GATnn'nnATC

Cuts at:      0    1376    1392 
   Size:       1376      16
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     675    1392 
   Size:        675     717
 
BsiEI CG_ry'CG

Cuts at:      0    1269    1392 
   Size:       1269     123
 
BsiHKAI G_wGCw'C

Cuts at:      0     117    1392 
   Size:        117    1275
 
BsmBI CGTCTCn'nnnn_

Cuts at:      0     250    1392 
   Size:        250    1142
 
Bsp1286I G_dGCh'C

Cuts at:      0     117    1207    1392 
   Size:        117    1090     185

  Fragments arranged by size: 

                1090     185     117
 
BspHI T'CATG_A

Cuts at:      0     423    1392 
   Size:        423     969
 
BsrFI r'CCGG_y

Cuts at:      0     846    1392 
   Size:        846     546
 
BsrGI T'GTAC_A

Cuts at:      0     543     779    1392 
   Size:        543     236     613

  Fragments arranged by size: 

                 613     543     236
 
BssSI C'ACGA_G

Cuts at:      0     908    1392 
   Size:        908     484
 
BstYI r'GATC_y

Cuts at:      0     103     519    1392 
   Size:        103     416     873

  Fragments arranged by size: 

                 873     416     103
 
BtgI C'CryG_G

Cuts at:      0     263     536    1392 
   Size:        263     273     856

  Fragments arranged by size: 

                 856     273     263
 
DraI TTT'AAA

Cuts at:      0     195    1392 
   Size:        195    1197
 
EaeI y'GGCC_r

Cuts at:      0     848    1392 
   Size:        848     544
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     188     766    1015    1108    1392 
   Size:        188     578     249      93     284

  Fragments arranged by size: 

                 578     284     249     188      93
 
EcoO109I rG'GnC_Cy

Cuts at:      0     199    1203    1204    1392 
   Size:        199    1004       1     188

  Fragments arranged by size: 

                1004     199     188       1
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0     266     298    1276    1308    1392 
   Size:        266      32     978      32      84

  Fragments arranged by size: 

                 978     266      84      32      32
 
HindIII A'AGCT_T

Cuts at:      0     570    1392 
   Size:        570     822
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0     347    1392 
   Size:        347    1045
 
MspA1I CmG'CkG

Cuts at:      0    1006    1392 
   Size:       1006     386
 
NcoI C'CATG_G

Cuts at:      0     263     536    1392 
   Size:        263     273     856

  Fragments arranged by size: 

                 856     273     263
 
NdeI CA'TA_TG

Cuts at:      0     933    1392 
   Size:        933     459
 
NspI r_CATG'y

Cuts at:      0     221     554    1392 
   Size:        221     333     838

  Fragments arranged by size: 

                 838     333     221
 
PciI A'CATG_T

Cuts at:      0     550    1392 
   Size:        550     842
 
PmlI CAC'GTG

Cuts at:      0       9    1392 
   Size:          9    1383
 
PspOMI G'GGCC_C

Cuts at:      0    1203    1392 
   Size:       1203     189
 
PvuII CAG'CTG

Cuts at:      0    1006    1392 
   Size:       1006     386
 
SfcI C'TryA_G

Cuts at:      0     434    1392 
   Size:        434     958
 
SmlI C'TyrA_G

Cuts at:      0     523    1392 
   Size:        523     869
 
SspI AAT'ATT

Cuts at:      0    1332    1392 
   Size:       1332      60
 
StyI C'CwwG_G

Cuts at:      0     263     536    1392 
   Size:        263     273     856

  Fragments arranged by size: 

                 856     273     263
 
TatI w'GTAC_w

Cuts at:      0     543     779    1392 
   Size:        543     236     613

  Fragments arranged by size: 

                 613     543     236
 
Tth111I GACn'n_nGTC

Cuts at:      0    1169    1392 
   Size:       1169     223
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:      0     858    1392 
   Size:        858     534
 

 Enzymes that do cut:

     AccI   AflIII    AlwNI     ApaI     ApoI     AseI     AvaI     BanI
    BanII    BciVI    BglII Bme1580I     BmrI     BpmI    BpuEI    BsaAI
    BsaBI     BsgI    BsiEI  BsiHKAI    BsmBI Bsp1286I    BspHI    BsrFI
    BsrGI    BssSI    BstYI     BtgI     DraI     EaeI  Eco57MI EcoO109I
    Hin4I  HindIII     MmeI   MspA1I     NcoI     NdeI     NspI     PciI
     PmlI   PspOMI    PvuII     SfcI     SmlI     SspI     StyI     TatI
  Tth111I     XcmI

 Enzymes that do not cut: 

     AarI    AatII   Acc65I     AclI     AcuI     AfeI    AflII     AgeI
     AhdI     AleI     AlfI     AloI    ApaLI     AscI    AsiSI    AvrII
     BaeI    BamHI     BbeI     BbsI    BbvCI     BcgI     BclI    BfrBI
     BglI     BlpI    BmgBI     BmtI     BplI   Bpu10I     BsaI    BsaHI
    BsaWI    BsaXI    BseRI    BseYI    BsiWI     BsmI    BspEI    BspMI
    BsrBI    BsrDI   BssHII   BstAPI    BstBI   BstEII    BstXI  BstZ17I
   Bsu36I    BtgZI     BtsI     ClaI    CspCI   DraIII     DrdI     EagI
     EarI     EciI  EcoICRI    EcoNI    EcoRI    EcoRV     FalI     FseI
     FspI    FspAI    HaeII   HincII     HpaI     KasI     KpnI     MfeI
     MluI     MscI     MslI     NaeI     NarI   NgoMIV     NheI     NotI
     NruI     NsiI     PacI     PasI    PflMI     PfoI     PmeI     PpiI
    PpuMI    PshAI     PsiI    PspXI     PsrI     PstI     PvuI    RsrII
     SacI    SacII     SalI    SanDI     SapI     SbfI     ScaI    SexAI
     SfiI     SfoI     SgfI    SgrAI     SmaI    SnaBI     SpeI     SphI
     SrfI     StuI     SwaI    TaqII     TstI     XbaI     XhoI     XmaI
     XmnI     ZraI