Restriction map for
pFN21AE2327
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# NO CUT ENZYMES ( 106 enzymes) #
##################################
AatII Acc65I AcyI AflII AflIII
AgeI AhdI Alw44I Aor51HI ApaI
ApaLI AscI Asp718I AspEI AspI
AviII AvrII BamHI BanII BbeI
BbrPI BbvCI BcgI BfrI BglI
BglII BlnI BsaAI BsaHI BsaI
BsiEI BsiWI BspDI BspHI BspLU11I
BspMI BsrBI BssHII BssSI Bst1107I
BstBI BstZ17I CfoI ClaI CpoI
DraIII DrdI DsaI EagI Eam1105I
EclXI Eco47III Eco52I EcoNI EcoT22I
FseI FspI HaeII HhaI Hin1I
HinP1I HindIII KasI KpnI KspI
MfeI MluI MunI NarI NcoI
NdeI NheI NotI NruI NsiI
NspV PacI PaeR7I PflFI PinAI
PmaCI PmeI PmlI PshAI PvuI
PvuII RcaI RsrII SacI SacII
SalI SapI SbfI SfiI SfoI
SfuI SgrAI SnaBI SpeI SphI
Sse8387I StuI SwaI Tth111I XcmI
XhoI
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# ONE CUT ENZYMES ( 57 enzymes) #
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AccI 2812 GTAGAC GT^[AC][GT]AC
AccIII 106 TCCGGA T^CCGGA
AclI 1322 AACGTT AA^CGTT
AocI 355 CCTGAGG CC^T[ACGT]AGG
AseI 1971 ATTAAT AT^TAAT
AsnI 1971 ATTAAT AT^TAAT
AspHI 2126 GAGCAC G[AT]GC[AT]^C
AvaI 160 CCCGGG C^[CT]CG[GA]G
BalI 1327 TGGCCA TGG^CCA
BanI 164 GGCACC G^G[CT][GA]CC
BciVI 367 GGATAC GTATCC(6/5)
BclI 2870 TGATCA T^GATCA
BlpI 2317 GCTGAGC GC^T[ACGT]AGC
Bpu1102I 2317 GCTGAGC GC^T[ACGT]AGC
BseAI 106 TCCGGA T^CCGGA
BseRI 110 GAGGAG GAGGAG(10/8)
BsiHKAI 2126 GAGCAC G[AT]GC[AT]^C
BsoBI 160 CCCGGG C^[CT]CG[GA]G
BspEI 106 TCCGGA T^CCGGA
BsrFI 152 GCCGGC [GA]^CCGG[CT]
BstEII 94 GGTAACC G^GT[ACGT]ACC
BstPI 94 GGTAACC G^GT[ACGT]ACC
BstXI 2200 CCACTGGGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
Bsu36I 355 CCTGAGG CC^T[ACGT]AGG
CelII 2317 GCTGAGC GC^T[ACGT]AGC
Cfr10I 152 GCCGGC [GA]^CCGG[CT]
DraII 39 GGGTCCC [GA]G^G[ACGT]CC[CT]
EaeI 1327 TGGCCA [CT]^GGCC[GA]
Eco81I 355 CCTGAGG CC^T[ACGT]AGG
EcoO109I 39 GGGTCCC [GA]G^G[ACGT]CC[CT]
EcoO65I 94 GGTAACC G^GT[ACGT]ACC
EcoRI 297 GAATTC G^AATTC
EcoT14I 901 CCAAGG C^C[AT][AT]GG
FauI 44 CCCGC CCCGC(4/6)
FbaI 2870 TGATCA T^GATCA
HgaI 1722 GACGC GACGC(5/10)
HincII 1591 GTTAAC GT[CT]^[GA]AC
HindII 1591 GTTAAC GT[CT]^[GA]AC
HpaI 1591 GTTAAC GTT^AAC
MluNI 1327 TGGCCA TGG^CCA
MroI 106 TCCGGA T^CCGGA
MscI 1327 TGGCCA TGG^CCA
MslI 256 CACATGGATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
MspA1I 1760 CCGCTG C[AC]G^C[GT]G
NaeI 152 GCCGGC GCC^GGC
NgoMIV 152 GCCGGC G^CCGGC
PflMI 2098 CCACATATTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PpuMI 39 GGGTCCC [GA]G^G[AT]CC[CT]
PshBI 1971 ATTAAT AT^TAAT
Psp1406I 1322 AACGTT AA^CGTT
SmaI 160 CCCGGG CCC^GGG
SmlI 713 CTCAAG C^T[CT][GA]AG
StyI 901 CCAAGG C^C[AT][AT]GG
Van91I 2098 CCACATATTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XbaI 1866 TCTAGA T^CTAGA
XmaCI 160 CCCGGG C^CCGGG
XmaI 160 CCCGGG C^CCGGG
###################################
# TWO CUT ENZYMES ( 27 enzymes) #
###################################
AccII 178 CGCG CG^CG
198 CGCG
Asp700I 1355 GAAGGTATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
2260 GAATATTTTC
AvaII 40 GGTCC G^G[AT]CC
1977 GGACC
BbsI 1862 GTCTTC GAAGAC(2/6)
2271 GTCTTC
BcnI 160 CCCGG CC^[GC]GG
161 CCGGG
BmyI 163 GGGCAC G[AGT]GC[ACT]^C
2126 GAGCAC
BpuAI 1862 GTCTTC GAAGAC(2/6)
2271 GTCTTC
BsgI 2616 CTGCAC GTGCAG(16/14)
2843 GTGCAG
BsmBI 883 CGTCTC CGTCTC(1/5)
1720 GAGACG
BsmFI 1493 GGGAC GGGAC(10/14)
41 GTCCC
BsmI 632 GAATGC GAATGC(1/-1)
1107 GAATGC
Bsp1286I 163 GGGCAC G[AGT]GC[ACT]^C
2126 GAGCAC
Bsp1407I 285 TGTACA T^GTACA
928 TGTACA
BsrDI 1656 CATTGC GCAATG(2/0)
1828 CATTGC
BsrGI 285 TGTACA T^GTACA
928 TGTACA
BstAPI 2286 GCAGATGCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
2731 GCAAGAATTGC
BstUI 178 CGCG CG^CG
198 CGCG
DraI 1540 TTTAAA TTT^AAA
1609 TTTAAA
EcoRV 339 GATATC GAT^ATC
872 GATATC
MaeII 1323 ACGT A^CGT
2226 ACGT
MvnI 178 CGCG CG^CG
198 CGCG
NciI 160 CCCGG CC^[GC]GG
161 CCGGG
NspI 188 GCATGT [GA]CATG^[CT]
1275 GCATGT
SexAI 1158 ACCTGGT A^CC[AT]GGT
1821 ACCTGGT
SspBI 285 TGTACA T^GTACA
928 TGTACA
Tsp45I 1527 GTCAC ^GT[GC]AC
2657 GTGAC
XmnI 1355 GAAGGTATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
2260 GAATATTTTC
#########################################
# 3 OR MORE CUT ENZYMES ( 68 enzymes) #
#########################################
AciI AcsI AfaI AluI AlwI
AlwNI ApoI BbvI BfaI BpmI
BsaBI BsaJI BsaWI BsiYI BslI
BsmAI BsrI BssKI BstF5I BstNI
BstYI Cac8I Cfr13I DdeI DpnII
EarI EcoRII Fnu4HI FokI HaeIII
HapII HinfI HpaII HphI ItaI
Ksp632I MaeI MaeIII MamI MboI
MboII MflI MnlI MseI MspI
MvaI MwoI NdeII NlaIII NlaIV
PleI PstI RsaI Sau3AI Sau96I
ScaI ScrFI SfaNI SfcI SspI
TaqI TfiI Tru9I TseI Tsp509I
TspRI TthHB8I XhoII