(Circular) (Six-base) MAPSORT of: pFN21AE2327.seq Check: 850 from: 1 to: 7642 pFN21AE2327 7642 bp With 182 enzymes: SgfI * February 11, 2011 18:42 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 5452 278 Size: 53 83 186 936 3916 2468 Fragments arranged by size: 3916 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 4818 4985 1937 Size: 2881 167 4594 Fragments arranged by size: 4594 2881 167 Acc65I G'GTAC_C Cuts at: 4963 4963 Size: 7642 AclI AA'CG_TT Cuts at: 1563 3328 5769 6142 1563 Size: 1765 2441 373 3063 Fragments arranged by size: 3063 2441 1765 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2403 2936 5705 7089 7357 1632 Size: 771 533 2769 1384 268 1917 Fragments arranged by size: 2769 1917 1384 771 533 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 7445 AflIII A'CryG_T Cuts at: 1203 6541 7518 1203 Size: 5338 977 1327 Fragments arranged by size: 5338 1327 977 AgeI A'CCGG_T Cuts at: 6473 6473 Size: 7642 AhdI GACnn_n'nnGTC Cuts at: 6371 6371 Size: 7642 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 7642 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 4156 1184 Size: 2972 4670 AloI GAACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2902 2934 2902 Size: 32 7610 AlwNI CAG_nnn'CTG Cuts at: 2299 4297 4845 5385 6957 2299 Size: 1998 548 540 1572 2984 Fragments arranged by size: 2984 1998 1572 548 540 ApaLI G'TGCA_C Cuts at: 5699 6855 5699 Size: 1156 6486 ApoI r'AATT_y Cuts at: 1493 2302 2656 2883 3421 3557 3894 4387 4452 Size: 809 354 227 538 136 337 493 65 Cuts at: 4452 4462 4951 5177 5329 7438 1493 Size: 10 489 226 152 2109 1697 Fragments arranged by size: 2109 1697 809 538 493 489 354 337 227 226 152 136 65 10 AseI AT'TA_AT Cuts at: 160 1032 3977 6196 160 Size: 872 2945 2219 1606 Fragments arranged by size: 2945 2219 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 7642 AvaI C'yCGr_G Cuts at: 1870 1943 2165 4967 1870 Size: 73 222 2802 4545 Fragments arranged by size: 4545 2802 222 73 BaeI ACnnnnGTAyCnnnnnnn_nnnnn' Cuts at: 2370 2403 2370 Size: 33 7609 BamHI G'GATC_C Cuts at: 4972 5339 4972 Size: 367 7275 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2169 4963 6418 618 Size: 325 217 627 382 2794 1455 1842 Fragments arranged by size: 2794 1842 1455 627 382 325 217 BanII G_rGCy'C Cuts at: 1391 4961 1391 Size: 3570 4072 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 3860 4269 928 Size: 971 1961 409 4301 Fragments arranged by size: 4301 1961 971 409 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 5833 5867 1903 Size: 34 3896 34 3678 Fragments arranged by size: 3896 3678 34 34 BciVI GTATCCnnnnn_n' Cuts at: 2366 5534 6744 2366 Size: 3168 1210 3264 Fragments arranged by size: 3264 3168 1210 BclI T'GATC_A Cuts at: 1548 4875 1548 Size: 3327 4315 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 6253 136 Size: 107 122 71 631 605 4581 1525 Fragments arranged by size: 4581 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 7637 7637 Size: 7642 BlpI GC'TnA_GC Cuts at: 4323 5059 4323 Size: 736 6906 Bme1580I G_kGCm'C Cuts at: 1790 2172 5703 6859 1790 Size: 382 3531 1156 2573 Fragments arranged by size: 3531 2573 1156 382 BmgBI CAC'GTC Cuts at: 1318 4232 1318 Size: 2914 4728 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2038 4148 4217 Size: 416 53 83 399 24 621 2110 69 Cuts at: 4217 5373 6331 442 Size: 1156 958 1753 Fragments arranged by size: 2110 1753 1156 958 621 416 399 83 69 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 7642 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2876 2945 4716 6302 1239 Size: 1637 69 1771 1586 2579 Fragments arranged by size: 2579 1771 1637 1586 69 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2703 5767 6632 6930 2703 Size: 3064 865 298 3415 Fragments arranged by size: 3415 3064 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 6305 882 Size: 617 4806 2219 Fragments arranged by size: 4806 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 5433 493 Size: 711 4229 2702 Fragments arranged by size: 4229 2702 711 BsaBI GATnn'nnATC Cuts at: 2670 3879 4686 7636 2670 Size: 1209 807 2950 2676 Fragments arranged by size: 2950 2676 1209 807 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 5449 5831 6466 275 Size: 53 83 186 936 3916 382 635 1451 Fragments arranged by size: 3916 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 2057 2111 3178 6075 6473 6747 6894 7305 2057 Size: 54 1067 2897 398 274 147 411 2394 Fragments arranged by size: 2897 2394 1067 411 398 274 147 54 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2093 2123 1178 Size: 30 165 30 690 30 6697 Fragments arranged by size: 6697 690 165 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2130 1186 Size: 944 6698 BseYI C'CCAG_C Cuts at: 3495 4212 4260 6845 7341 3495 Size: 717 48 2585 496 3796 Fragments arranged by size: 3796 2585 717 496 48 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 4606 4869 1163 Size: 573 2870 263 3936 Fragments arranged by size: 3936 2870 573 263 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 5092 5853 6881 1540 Size: 275 186 3091 761 1028 2301 Fragments arranged by size: 3091 2301 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 4135 5703 5788 6859 4135 Size: 1568 85 1071 4918 Fragments arranged by size: 4918 1568 1071 85 BsmI GAATG_Cn' Cuts at: 2643 3118 5153 5246 2643 Size: 475 2035 93 5039 Fragments arranged by size: 5039 2035 475 93 BsmBI CGTCTCn'nnnn_ Cuts at: 2894 3719 2894 Size: 825 6817 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2172 4135 4961 5703 5788 6859 1391 Size: 399 382 1963 826 742 85 1071 2174 Fragments arranged by size: 2174 1963 1071 826 742 399 382 85 BspEI T'CCGG_A Cuts at: 2111 7305 2111 Size: 5194 2448 BspHI T'CATG_A Cuts at: 5531 7261 5531 Size: 1730 5912 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 4997 844 Size: 4153 3489 BsrBI CCG'CTC Cuts at: 1394 1823 5529 1394 Size: 429 3706 3507 Fragments arranged by size: 3706 3507 429 BsrDI GCAATG_nn' Cuts at: 65 1248 3660 3832 6137 6311 65 Size: 1183 2412 172 2305 174 1396 Fragments arranged by size: 2412 2305 1396 1183 174 172 BsrFI r'CCGG_y Cuts at: 1700 1810 2157 6286 6473 1700 Size: 110 347 4129 187 2869 Fragments arranged by size: 4129 2869 347 187 110 BsrGI T'GTAC_A Cuts at: 96 2290 2933 96 Size: 2194 643 4805 Fragments arranged by size: 4805 2194 643 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 7642 BssSI C'ACGA_G Cuts at: 5702 6714 5702 Size: 1012 6630 BstAPI GCAn_nnn'nTGC Cuts at: 794 4297 4742 794 Size: 3503 445 3694 Fragments arranged by size: 3694 3503 445 BstBI TT'CG_AA Cuts at: 6446 6446 Size: 7642 BstEII G'GTnAC_C Cuts at: 1184 2099 1184 Size: 915 6727 BstXI CCAn_nnnn'nTGG Cuts at: 4212 4212 Size: 7642 BstYI r'GATC_y Cuts at: 1972 2765 4189 4369 4972 5339 5724 5741 7193 Size: 793 1424 180 603 367 385 17 1452 Cuts at: 7193 7279 7291 7637 1972 Size: 86 12 346 1977 Fragments arranged by size: 1977 1452 1424 793 603 385 367 346 180 86 17 12 Bsu36I CC'TnA_GG Cuts at: 2361 2361 Size: 7642 BtgI C'CryG_G Cuts at: 513 1065 513 Size: 552 7090 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 6903 Fragments arranged by size: 6903 683 56 BtsI GCAGTG_nn' Cuts at: 2866 4008 4286 5158 5952 5972 2866 Size: 1142 278 872 794 20 4536 Fragments arranged by size: 4536 1142 872 794 278 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 7642 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 7607 DraI TTT'AAA Cuts at: 3547 3616 4946 3547 Size: 69 1330 6243 Fragments arranged by size: 6243 1330 69 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 6649 809 Size: 507 5333 1802 Fragments arranged by size: 5333 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 3332 5089 5978 Size: 54 1425 50 275 18 1502 1757 889 Cuts at: 5978 7607 8 Size: 1629 43 Fragments arranged by size: 1757 1629 1502 1425 889 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 5089 1537 Size: 275 3277 4090 Fragments arranged by size: 4090 3277 275 EarI CTCTTCn'nnn_ Cuts at: 1353 3456 3811 4212 5572 1353 Size: 2103 355 401 1360 3423 Fragments arranged by size: 3423 2103 1360 401 355 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2145 4902 6229 6603 6749 387 Size: 1064 138 556 2757 1327 374 146 1280 Fragments arranged by size: 2757 1327 1280 1064 556 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2403 2876 2936 2945 4716 5705 6302 Size: 393 771 473 60 9 1771 989 597 Cuts at: 6302 7089 7357 1239 Size: 787 268 1524 Fragments arranged by size: 1771 1524 989 787 771 597 473 393 268 60 9 EcoO109I rG'GnC_Cy Cuts at: 2045 2045 Size: 7642 EcoRI G'AATT_C Cuts at: 2302 4951 5329 2302 Size: 2649 378 4615 Fragments arranged by size: 4615 2649 378 EcoRV GAT'ATC Cuts at: 2346 2879 2346 Size: 533 7109 FspI TGC'GCA Cuts at: 6148 6148 Size: 7642 HaeII r_GCGC'y Cuts at: 1678 6789 1678 Size: 5111 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2315 2347 3790 3822 3974 Size: 32 135 32 743 32 1443 32 152 Cuts at: 3974 4006 4361 4393 4789 4820 4821 4852 6283 Size: 32 355 32 396 31 1 31 1431 Cuts at: 6283 6315 6357 6389 1373 Size: 32 42 32 2626 Fragments arranged by size: 2626 1443 1431 743 396 355 152 135 42 32 32 32 32 32 32 32 32 31 31 1 HincII GTy'rAC Cuts at: 669 1657 1938 3598 4986 5232 669 Size: 988 281 1660 1388 246 3079 Fragments arranged by size: 3079 1660 1388 988 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 7642 HpaI GTT'AAC Cuts at: 3598 5232 3598 Size: 1634 6008 KpnI G_GTAC'C Cuts at: 4967 4967 Size: 7642 MfeI C'AATT_G Cuts at: 5241 5241 Size: 7642 MluI A'CGCG_T Cuts at: 7518 7518 Size: 7642 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 6756 6940 1305 Size: 5451 184 2007 Fragments arranged by size: 5451 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 3334 10 Size: 54 1425 343 1502 4318 Fragments arranged by size: 4318 1502 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2265 5600 5959 6118 518 Size: 648 1099 3335 359 159 2042 Fragments arranged by size: 3335 2042 1099 648 359 159 MspA1I CmG'CkG Cuts at: 1606 3767 5058 5735 6883 7128 1606 Size: 2161 1291 677 1148 245 2120 Fragments arranged by size: 2161 2120 1291 1148 677 245 NaeI GCC'GGC Cuts at: 2159 2159 Size: 7642 NcoI C'CATG_G Cuts at: 513 1065 513 Size: 552 7090 NdeI CA'TA_TG Cuts at: 387 387 Size: 7642 NgoMIV G'CCGG_C Cuts at: 2157 2157 Size: 7642 NheI G'CTAG_C Cuts at: 1052 1052 Size: 7642 NotI GC'GGCC_GC Cuts at: 5089 5089 Size: 7642 NspI r_CATG'y Cuts at: 1132 1229 2197 3284 5000 6545 1132 Size: 97 968 1087 1716 1545 2229 Fragments arranged by size: 2229 1716 1545 1087 968 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 7642 PciI A'CATG_T Cuts at: 6541 6541 Size: 7642 PflMI CCAn_nnn'nTGG Cuts at: 1108 4109 1108 Size: 3001 4641 PfoI T'CCnGG_A Cuts at: 2430 4923 2430 Size: 2493 5149 PmeI GTTT'AAAC Cuts at: 4946 4946 Size: 7642 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 5711 5743 7224 7256 5711 Size: 32 1481 32 6097 Fragments arranged by size: 6097 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2045 2045 Size: 7642 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 7642 PsiI TTA'TAA Cuts at: 5212 5212 Size: 7642 PsrI GAACnnnnnnTACnnnnnnn_nnnnn' Cuts at: 2274 2306 3909 3941 2274 Size: 32 1603 32 5975 Fragments arranged by size: 5975 1603 32 32 PstI C_TGCA'G Cuts at: 830 2839 3030 4293 4994 830 Size: 2009 191 1263 701 3478 Fragments arranged by size: 3478 2009 1263 701 191 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 7642 SalI G'TCGA_C Cuts at: 1936 4984 1936 Size: 3048 4594 SanDI GG'GwC_CC Cuts at: 2045 2045 Size: 7642 SbfI CC_TGCA'GG Cuts at: 4994 4994 Size: 7642 ScaI AGT'ACT Cuts at: 2872 3376 4158 4854 5890 7602 2872 Size: 504 782 696 1036 1712 2912 Fragments arranged by size: 2912 1712 1036 782 696 504 SexAI A'CCwGG_T Cuts at: 3163 3826 3163 Size: 663 6979 SfcI C'TryA_G Cuts at: 826 1045 2253 2835 3026 3378 3591 4289 4704 Size: 219 1208 582 191 352 213 698 415 Cuts at: 4704 4815 4990 6125 6806 6997 826 Size: 111 175 1135 681 191 1471 Fragments arranged by size: 1471 1208 1135 698 681 582 415 352 219 213 191 191 175 111 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 7642 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 7642 SmaI CCC'GGG Cuts at: 1872 2167 4969 1872 Size: 295 2802 4545 Fragments arranged by size: 4545 2802 295 SmlI C'TyrA_G Cuts at: 820 1017 1943 2718 5746 6647 6909 820 Size: 197 926 775 3028 901 262 1553 Fragments arranged by size: 3028 1553 926 901 775 262 197 SnaBI TAC'GTA Cuts at: 493 5433 493 Size: 4940 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 7642 SphI G_CATG'C Cuts at: 1132 5000 1132 Size: 3868 3774 SspI AAT'ATT Cuts at: 5 52 3093 3134 4268 5566 5 Size: 47 3041 41 1134 1298 2081 Fragments arranged by size: 3041 2081 1298 1134 47 41 StuI AGG'CCT Cuts at: 1517 1517 Size: 7642 StyI C'CwwG_G Cuts at: 513 1065 2906 5081 513 Size: 552 1841 2175 3074 Fragments arranged by size: 3074 2175 1841 552 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2511 5669 5686 5839 6024 6469 325 Size: 1255 931 3158 17 153 185 445 1498 Fragments arranged by size: 3158 1498 1255 931 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2257 2290 2786 Size: 274 80 33 51 1443 280 33 496 Cuts at: 2786 2870 2933 3374 3755 4156 4852 5888 7600 Size: 84 63 441 381 401 696 1036 1712 Cuts at: 7600 96 Size: 138 Fragments arranged by size: 1712 1443 1036 696 496 441 401 381 280 274 138 84 80 63 51 33 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 7602 7634 7602 Size: 32 7610 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 7642 XbaI T'CTAG_A Cuts at: 3871 4978 3871 Size: 1107 6535 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 7642 XmaI C'CCGG_G Cuts at: 1870 2165 4967 1870 Size: 295 2802 4545 Fragments arranged by size: 4545 2802 295 XmnI GAAnn'nnTTC Cuts at: 3364 4269 5771 3364 Size: 905 1502 5235 Fragments arranged by size: 5235 1502 905 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 5450 276 Size: 53 83 186 936 3916 2468 Fragments arranged by size: 3916 2468 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AloI AlwNI ApaLI ApoI AseI AsiSI AvaI BaeI BamHI BanI BanII BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoO109I EcoRI EcoRV FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NcoI NdeI NgoMIV NheI NotI NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PsrI PstI PvuI SalI SanDI SbfI ScaI SexAI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI ApaI AscI AvrII BbeI BbvCI BfrBI BplI Bpu10I BsiWI BstZ17I DraIII EcoICRI EcoNI FalI FseI FspAI KasI NarI NruI NsiI PacI PmlI PspOMI PspXI PvuII RsrII SacI SacII SapI SfiI SfoI SrfI SwaI XcmI