################################## # NO CUT ENZYMES ( 133 enzymes) # ################################## AatII Acc65I AccII AccIII AciI AclI AcyI AflII AflIII AgeI AhdI Aor51HI ApaI AscI AseI AsnI Asp718I AspEI AvaI AviII AvrII BalI BamHI BanII BbeI BbrPI BbvCI BcgI BciVI BclI BfrI BglI BlnI BlpI BpmI Bpu1102I BsaAI BsaHI BsaWI BseAI BsiEI BsiWI BsmBI BsmI BsoBI BspDI BspEI BspHI BspLU11I BsrBI BsrFI BssHII BssSI BstAPI BstBI BstEII BstPI BstUI BstXI CelII CfoI Cfr10I ClaI CpoI DraIII EaeI EagI Eam1105I EclXI Eco47III Eco52I EcoNI EcoO65I FauI FbaI FseI FspI HaeII HgaI HhaI Hin1I HinP1I HindIII KasI KpnI KspI MaeII MluI MluNI MroI MscI MspA1I MvnI NaeI NarI NdeI NgoMIV NheI NotI NruI NspV PacI PaeR7I PinAI PmaCI PmeI PmlI PshAI PshBI Psp1406I PstI PvuI PvuII RcaI RsrII SacI SacII SalI SapI SbfI SfiI SfoI SfuI SgrAI SmaI SnaBI Sse8387I SspI SwaI XbaI XhoI XmaCI XmaI ################################### # ONE CUT ENZYMES ( 32 enzymes) # ################################### Alw44I 557 GTGCAC G^TGCAC AocI 2437 CCTAAGG CC^T[ACGT]AGG ApaLI 557 GTGCAC G^TGCAC BanI 2541 GGCACC G^G[CT][GA]CC BbvI 2524 GCTGC GCAGC(8/12) BsaI 621 GAGACC GGTCTC(1/5) BspMI 7 ACCTGC ACCTGC(4/8) BsrDI 190 CATTGC GCAATG(2/0) Bst1107I 392 GTATAC GTA^TAC BstZ17I 392 GTATAC GTA^TAC Bsu36I 2437 CCTAAGG CC^T[ACGT]AGG DrdI 2235 GACAGGGAAGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC DsaI 1735 CCATGG C^C[GA][CT]GG Eco81I 2437 CCTAAGG CC^T[ACGT]AGG EcoRI 2148 GAATTC G^AATTC EcoRV 1914 GATATC GAT^ATC Fnu4HI 2524 GCTGC GC^[ACGT]GC HincII 2703 GTTAAC GT[CT]^[GA]AC HindII 2703 GTTAAC GT[CT]^[GA]AC HpaI 2703 GTTAAC GTT^AAC ItaI 2524 GCTGC GC^[ACGT]GC MwoI 877 GCAATTACAGC GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC NcoI 1735 CCATGG C^CATGG NspI 858 GCATGC [GA]CATG^[CT] PflMI 1951 CCAAATGGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG ScaI 2425 AGTACT AGT^ACT SexAI 2418 ACCTGGT A^CC[AT]GGT SphI 858 GCATGC GCATG^C StuI 2441 AGGCCT AGG^CCT TseI 2524 GCTGC G^C[AT]GC Tsp45I 1196 GTCAC ^GT[GC]AC Van91I 1951 CCAAATGGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG ################################### # TWO CUT ENZYMES ( 32 enzymes) # ################################### AccI 76 GTCTAC GT^[AC][GT]AC 392 GTATAC AcsI 801 AAATTC [GA]^AATT[CT] 2148 GAATTC AlwNI 2597 CAGCTTCTG CAG[ACGT][ACGT][ACGT]^CTG 2857 CAGTGTCTG ApoI 801 AAATTC [GA]^AATT[CT] 2148 GAATTC AspHI 557 GTGCAC G[AT]GC[AT]^C 2384 GTGCTC AspI 640 GACACAGTC GAC[ACGT]^[ACGT][ACGT]GTC 2579 GACTTGGTC BcnI 402 CCGGG CC^[GC]GG 1503 CCGGG BglII 1141 AGATCT A^GATCT 2089 AGATCT BmyI 557 GTGCAC G[AGT]GC[ACT]^C 2384 GTGCTC BsaBI 1342 GATAACAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC 2090 GATCTAAATC BsgI 1640 CTGCAC GTGCAG(16/14) 2755 GTGCAG BsiHKAI 557 GTGCAC G[AT]GC[AT]^C 2384 GTGCTC BsmAI 621 GAGAC GTCTC(1/5) 1164 GTCTC Bsp1286I 557 GTGCAC G[AGT]GC[ACT]^C 2384 GTGCTC Bsp1407I 143 TGTACA T^GTACA 2076 TGTACA BsrGI 143 TGTACA T^GTACA 2076 TGTACA EcoT14I 713 CCAAGG C^C[AT][AT]GG 1735 CCATGG HapII 402 CCGG C^CGG 1503 CCGG HpaII 402 CCGG C^CGG 1503 CCGG MamI 1342 GATAACAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC 2090 GATCTAAATC MfeI 196 CAATTG C^AATTG 845 CAATTG MspI 402 CCGG C^CGG 1503 CCGG MunI 196 CAATTG C^AATTG 845 CAATTG NciI 402 CCGGG CC^[GC]GG 1503 CCGGG PflFI 640 GACACAGTC GAC[ACGT]^[ACGT][ACGT]GTC 2579 GACTTGGTC SfcI 78 CTACAG C^T[GA][CT]AG 2594 CTACAG SmlI 1166 CTCAAG C^T[CT][GA]AG 1886 CTCAAG SpeI 2428 ACTAGT A^CTAGT 2707 ACTAGT SspBI 143 TGTACA T^GTACA 2076 TGTACA StyI 713 CCAAGG C^C[AT][AT]GG 1735 CCATGG Tth111I 640 GACACAGTC GAC[ACGT]^[ACGT][ACGT]GTC 2579 GACTTGGTC XcmI 1817 CCAAAAAGGCTGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG 2035 CCAGAAAAATACTGG ######################################### # 3 OR MORE CUT ENZYMES ( 61 enzymes) # ######################################### AfaI AluI AlwI Asp700I AvaII BbsI BfaI BpuAI BsaJI BseRI BsiYI BslI BsmFI BsrI BssKI BstF5I BstNI BstYI Cac8I Cfr13I DdeI DpnII DraI DraII EarI EcoO109I EcoRII EcoT22I FokI HaeIII HinfI HphI Ksp632I MaeI MaeIII MboI MboII MflI MnlI MseI MslI MvaI NdeII NlaIII NlaIV NsiI PleI PpuMI RsaI Sau3AI Sau96I ScrFI SfaNI TaqI TfiI Tru9I Tsp509I TspRI TthHB8I XhoII XmnI