(Circular) (Six-base) MAPSORT of: pFN21AE6411.seq Check: 6259 from: 1 to: 6511 pFN21AE6411 6511 bp With 182 enzymes: SgfI * March 18, 2015 14:51 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 4321 278 Size: 53 83 186 936 2785 2468 Fragments arranged by size: 2785 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3854 1937 Size: 1917 4594 Acc65I G'GTAC_C Cuts at: 3832 3832 Size: 6511 AclI AA'CG_TT Cuts at: 1563 4638 5011 1563 Size: 3075 373 3063 Fragments arranged by size: 3075 3063 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2905 4574 5958 6226 1632 Size: 1273 1669 1384 268 1917 Fragments arranged by size: 1917 1669 1384 1273 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 6314 AflIII A'CryG_T Cuts at: 1203 5410 6387 1203 Size: 4207 977 1327 Fragments arranged by size: 4207 1327 977 AgeI A'CCGG_T Cuts at: 5342 5342 Size: 6511 AhdI GACnn_n'nnGTC Cuts at: 5240 5240 Size: 6511 AleI CACnn'nnGTG Cuts at: 1166 3149 1166 Size: 1983 4528 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 6511 AloI GAACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 3074 3106 3074 Size: 32 6479 AlwNI CAG_nnn'CTG Cuts at: 2549 2928 3007 4254 5826 2549 Size: 379 79 1247 1572 3234 Fragments arranged by size: 3234 1572 1247 379 79 ApaI G_GGCC'C Cuts at: 3015 3015 Size: 6511 ApaLI G'TGCA_C Cuts at: 2161 3515 4568 5724 2161 Size: 1354 1053 1156 2948 Fragments arranged by size: 2948 1354 1156 1053 ApoI r'AATT_y Cuts at: 1493 2824 3820 4046 4198 6307 1493 Size: 1331 996 226 152 2109 1697 Fragments arranged by size: 2109 1697 1331 996 226 152 AseI AT'TA_AT Cuts at: 160 1032 5065 160 Size: 872 4033 1606 Fragments arranged by size: 4033 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6511 AvaI C'yCGr_G Cuts at: 1870 1943 2976 3078 3836 1870 Size: 73 1033 102 758 4545 Fragments arranged by size: 4545 1033 758 102 73 BamHI G'GATC_C Cuts at: 3841 4208 3841 Size: 367 6144 BanI G'GyrC_C Cuts at: 618 943 1160 1787 3725 3832 5287 618 Size: 325 217 627 1938 107 1455 1842 Fragments arranged by size: 1938 1842 1455 627 325 217 107 BanII G_rGCy'C Cuts at: 1391 2977 3015 3246 3830 1391 Size: 1586 38 231 584 4072 Fragments arranged by size: 4072 1586 584 231 38 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2089 928 Size: 971 190 5350 Fragments arranged by size: 5350 971 190 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 4702 4736 1903 Size: 34 2765 34 3678 Fragments arranged by size: 3678 2765 34 34 BciVI GTATCCnnnnn_n' Cuts at: 4403 5613 4403 Size: 1210 5301 BclI T'GATC_A Cuts at: 1548 2295 1548 Size: 747 5764 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2009 2380 3032 Size: 107 122 71 631 605 337 371 652 Cuts at: 3032 5122 136 Size: 2090 1525 Fragments arranged by size: 2090 1525 652 631 605 371 337 122 107 71 BglII A'GATC_T Cuts at: 6506 6506 Size: 6511 BlpI GC'TnA_GC Cuts at: 2429 3928 2429 Size: 1499 5012 Bme1580I G_kGCm'C Cuts at: 1790 2165 2580 2744 2762 3015 3282 3393 3519 Size: 375 415 164 18 253 267 111 126 Cuts at: 3519 3545 3706 3728 4572 5728 1790 Size: 26 161 22 844 1156 2573 Fragments arranged by size: 2573 1156 844 415 375 267 253 164 161 126 111 26 22 18 BmgBI CAC'GTC Cuts at: 1318 2818 1318 Size: 1500 5011 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2248 3748 4242 Size: 416 53 83 399 24 831 1500 494 Cuts at: 4242 5200 442 Size: 958 1753 Fragments arranged by size: 1753 1500 958 831 494 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6511 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2647 2950 3401 5171 1239 Size: 1408 303 451 1770 2579 Fragments arranged by size: 2579 1770 1408 451 303 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2330 2777 3185 4636 5501 5799 2330 Size: 447 408 1451 865 298 3042 Fragments arranged by size: 3042 1451 865 447 408 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 3583 5174 882 Size: 617 2084 1591 2219 Fragments arranged by size: 2219 2084 1591 617 BsaAI yAC'GTr Cuts at: 493 1204 4302 493 Size: 711 3098 2702 Fragments arranged by size: 3098 2702 711 BsaBI GATnn'nnATC Cuts at: 2679 6505 2679 Size: 3826 2685 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 4318 4700 5335 275 Size: 53 83 186 936 2785 382 635 1451 Fragments arranged by size: 2785 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 4944 5342 5616 5763 6174 4944 Size: 398 274 147 411 5281 Fragments arranged by size: 5281 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2426 2456 2576 2606 1178 Size: 30 165 30 1023 30 120 30 5083 Fragments arranged by size: 5083 1023 165 120 30 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2330 3059 1186 Size: 1144 729 4638 Fragments arranged by size: 4638 1144 729 BseYI C'CCAG_C Cuts at: 2519 3102 3721 5714 6210 2519 Size: 583 619 1993 496 2820 Fragments arranged by size: 2820 1993 619 583 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2312 3356 1163 Size: 573 576 1044 4318 Fragments arranged by size: 4318 1044 576 573 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3961 4722 5750 1540 Size: 275 186 1960 761 1028 2301 Fragments arranged by size: 2301 1960 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2165 3519 4572 4657 5728 2165 Size: 1354 1053 85 1071 2948 Fragments arranged by size: 2948 1354 1071 1053 85 BsmI GAATG_Cn' Cuts at: 4022 4115 4022 Size: 93 6418 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2165 2580 2744 2762 2977 3015 3246 Size: 399 375 415 164 18 215 38 231 Cuts at: 3246 3282 3393 3519 3545 3706 3728 3830 4572 Size: 36 111 126 26 161 22 102 742 Cuts at: 4572 4657 5728 1391 Size: 85 1071 2174 Fragments arranged by size: 2174 1071 742 415 399 375 231 215 164 161 126 111 102 85 38 36 26 22 18 BspEI T'CCGG_A Cuts at: 6174 6174 Size: 6511 BspHI T'CATG_A Cuts at: 4400 6130 4400 Size: 1730 4781 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2334 3866 844 Size: 1490 1532 3489 Fragments arranged by size: 3489 1532 1490 BsrBI CCG'CTC Cuts at: 1394 1823 2077 2179 4398 1394 Size: 429 254 102 2219 3507 Fragments arranged by size: 3507 2219 429 254 102 BsrDI GCAATG_nn' Cuts at: 65 1248 2463 3770 5006 5180 65 Size: 1183 1215 1307 1236 174 1396 Fragments arranged by size: 1396 1307 1236 1215 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2089 2331 2374 2653 3146 3481 5155 Size: 110 279 242 43 279 493 335 1674 Cuts at: 5155 5342 1700 Size: 187 2869 Fragments arranged by size: 2869 1674 493 335 279 279 242 187 110 43 BsrGI T'GTAC_A Cuts at: 96 96 Size: 6511 BssHII G'CGCG_C Cuts at: 1925 2043 1925 Size: 118 6393 BssSI C'ACGA_G Cuts at: 4571 5583 4571 Size: 1012 5499 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 6511 BstBI TT'CG_AA Cuts at: 2509 5315 2509 Size: 2806 3705 BstEII G'GTnAC_C Cuts at: 1184 2401 1184 Size: 1217 5294 BstYI r'GATC_y Cuts at: 1972 2349 2647 2892 3841 4208 4593 4610 6062 Size: 377 298 245 949 367 385 17 1452 Cuts at: 6062 6148 6160 6506 1972 Size: 86 12 346 1977 Fragments arranged by size: 1977 1452 949 385 377 367 346 298 245 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2004 2580 3318 513 Size: 552 939 576 738 3706 Fragments arranged by size: 3706 939 738 576 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 3412 3583 487 Size: 683 56 2186 171 3415 Fragments arranged by size: 3415 2186 683 171 56 BtsI GCAGTG_nn' Cuts at: 2445 2816 4027 4821 4841 2445 Size: 371 1211 794 20 4115 Fragments arranged by size: 4115 1211 794 371 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6511 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 3765 3800 529 Size: 35 3201 35 3240 Fragments arranged by size: 3240 3201 35 35 DraI TTT'AAA Cuts at: 3815 3815 Size: 6511 DraIII CAC_nnn'GTG Cuts at: 2575 2575 Size: 6511 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 5518 809 Size: 507 4202 1802 Fragments arranged by size: 4202 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2025 2301 2376 Size: 54 1425 50 275 18 195 276 75 Cuts at: 2376 2494 2785 3321 3498 3786 3958 4847 6476 Size: 118 291 536 177 288 172 889 1629 Cuts at: 6476 8 Size: 43 Fragments arranged by size: 1629 1425 889 536 291 288 276 275 195 177 172 118 75 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3958 1537 Size: 275 2146 4090 Fragments arranged by size: 4090 2146 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2285 2529 3077 3119 4441 1353 Size: 932 244 548 42 1322 3423 Fragments arranged by size: 3423 1322 932 548 244 42 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 3494 5098 5472 5618 387 Size: 1064 138 1905 1604 374 146 1280 Fragments arranged by size: 1905 1604 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2647 2905 2950 3401 4574 5171 5958 Size: 393 1015 258 45 451 1173 597 787 Cuts at: 5958 6226 1239 Size: 268 1524 Fragments arranged by size: 1524 1173 1015 787 597 451 393 268 258 45 EcoO109I rG'GnC_Cy Cuts at: 2170 2515 3011 3082 3192 2170 Size: 345 496 71 110 5489 Fragments arranged by size: 5489 496 345 110 71 EcoRI G'AATT_C Cuts at: 3820 4198 3820 Size: 378 6133 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2818 2818 Size: 6511 FspI TGC'GCA Cuts at: 5017 5017 Size: 6511 HaeII r_GCGC'y Cuts at: 1678 2015 2195 2310 5658 1678 Size: 337 180 115 3348 2531 Fragments arranged by size: 3348 2531 337 180 115 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 3737 3769 5152 5184 5226 Size: 32 135 32 2165 32 1383 32 42 Cuts at: 5226 5258 1373 Size: 32 2626 Fragments arranged by size: 2626 2165 1383 135 42 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3855 4101 669 Size: 988 281 1917 246 3079 Fragments arranged by size: 3079 1917 988 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 6511 HpaI GTT'AAC Cuts at: 4101 4101 Size: 6511 KpnI G_GTAC'C Cuts at: 3836 3836 Size: 6511 MfeI C'AATT_G Cuts at: 4110 4110 Size: 6511 MluI A'CGCG_T Cuts at: 6387 6387 Size: 6511 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2262 2344 2666 5625 5809 1305 Size: 957 82 322 2959 184 2007 Fragments arranged by size: 2959 2007 957 322 184 82 MscI TGG'CCA Cuts at: 10 64 1489 1832 2303 2496 2787 3323 3500 Size: 54 1425 343 471 193 291 536 177 Cuts at: 3500 3788 10 Size: 288 2733 Fragments arranged by size: 2733 1425 536 471 343 291 288 193 177 54 MslI CAynn'nnrTG Cuts at: 518 1166 2150 2450 2585 3149 3419 4469 4828 Size: 648 984 300 135 564 270 1050 359 Cuts at: 4828 4987 518 Size: 159 2042 Fragments arranged by size: 2042 1050 984 648 564 359 300 270 159 135 MspA1I CmG'CkG Cuts at: 1606 2024 2124 2546 3370 3721 3927 4604 5752 Size: 418 100 422 824 351 206 677 1148 Cuts at: 5752 5997 1606 Size: 245 2120 Fragments arranged by size: 2120 1148 824 677 422 418 351 245 206 100 NaeI GCC'GGC Cuts at: 2376 2655 2376 Size: 279 6232 NcoI C'CATG_G Cuts at: 513 1065 2004 3318 513 Size: 552 939 1314 3706 Fragments arranged by size: 3706 1314 939 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 6511 NgoMIV G'CCGG_C Cuts at: 2374 2653 2374 Size: 279 6232 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6511 NotI GC'GGCC_GC Cuts at: 3958 3958 Size: 6511 NspI r_CATG'y Cuts at: 1132 1229 3058 3869 5414 1132 Size: 97 1829 811 1545 2229 Fragments arranged by size: 2229 1829 1545 811 97 PasI CC'CwG_GG Cuts at: 1348 2624 1348 Size: 1276 5235 PciI A'CATG_T Cuts at: 5410 5410 Size: 6511 PflMI CCAn_nnn'nTGG Cuts at: 1108 3007 1108 Size: 1899 4612 PfoI T'CCnGG_A Cuts at: 2663 3732 2663 Size: 1069 5442 PmeI GTTT'AAAC Cuts at: 3815 3815 Size: 6511 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4580 4612 6093 6125 4580 Size: 32 1481 32 4966 Fragments arranged by size: 4966 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2515 3082 2515 Size: 567 5944 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 6511 PsiI TTA'TAA Cuts at: 4081 4081 Size: 6511 PspOMI G'GGCC_C Cuts at: 3011 3011 Size: 6511 PspXI vC'TCGA_Gb Cuts at: 2976 2976 Size: 6511 PstI C_TGCA'G Cuts at: 830 3863 830 Size: 3033 3478 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6511 PvuII CAG'CTG Cuts at: 2546 3370 3721 2546 Size: 824 351 5336 Fragments arranged by size: 5336 824 351 SalI G'TCGA_C Cuts at: 1936 3853 1936 Size: 1917 4594 SanDI GG'GwC_CC Cuts at: 3082 3082 Size: 6511 SbfI CC_TGCA'GG Cuts at: 3863 3863 Size: 6511 ScaI AGT'ACT Cuts at: 2258 4759 6471 2258 Size: 2501 1712 2298 Fragments arranged by size: 2501 2298 1712 SfcI C'TryA_G Cuts at: 826 1045 3859 4994 5675 5866 826 Size: 219 2814 1135 681 191 1471 Fragments arranged by size: 2814 1471 1135 681 219 191 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6511 SgrAI Cr'CCGG_yG Cuts at: 1700 2331 3146 1700 Size: 631 815 5065 Fragments arranged by size: 5065 815 631 SmaI CCC'GGG Cuts at: 1872 3080 3838 1872 Size: 1208 758 4545 Fragments arranged by size: 4545 1208 758 SmlI C'TyrA_G Cuts at: 820 1017 1943 2345 2792 2976 3200 4615 5516 Size: 197 926 402 447 184 224 1415 901 Cuts at: 5516 5778 820 Size: 262 1553 Fragments arranged by size: 1553 1415 926 901 447 402 262 224 197 184 SnaBI TAC'GTA Cuts at: 493 4302 493 Size: 3809 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 6511 SphI G_CATG'C Cuts at: 1132 3869 1132 Size: 2737 3774 SspI AAT'ATT Cuts at: 5 52 4435 5 Size: 47 4383 2081 Fragments arranged by size: 4383 2081 47 StuI AGG'CCT Cuts at: 1517 3648 1517 Size: 2131 4380 StyI C'CwwG_G Cuts at: 513 1065 2004 2112 3318 3783 3950 513 Size: 552 939 108 1206 465 167 3074 Fragments arranged by size: 3074 1206 939 552 465 167 108 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 3656 3745 3793 4538 4555 4708 4893 Size: 1255 2076 89 48 745 17 153 185 Cuts at: 4893 5338 325 Size: 445 1498 Fragments arranged by size: 2076 1498 1255 745 445 185 153 89 48 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2154 2256 3750 Size: 274 80 33 51 1443 177 102 1494 Cuts at: 3750 4757 6469 96 Size: 1007 1712 138 Fragments arranged by size: 1712 1494 1443 1007 274 177 138 102 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 6471 6503 6471 Size: 32 6479 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 6511 XbaI T'CTAG_A Cuts at: 3847 3847 Size: 6511 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2011 2386 3718 2011 Size: 375 1332 4804 Fragments arranged by size: 4804 1332 375 XhoI C'TCGA_G Cuts at: 1943 2976 1943 Size: 1033 5478 XmaI C'CCGG_G Cuts at: 1870 3078 3836 1870 Size: 1208 758 4545 Fragments arranged by size: 4545 1208 758 XmnI GAAnn'nnTTC Cuts at: 2702 4640 2702 Size: 1938 4573 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 4319 276 Size: 53 83 186 936 2785 2468 Fragments arranged by size: 2785 2468 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AloI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI Eco57MI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NcoI NdeI NgoMIV NheI NotI NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PspOMI PspXI PstI PvuI PvuII SalI SanDI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AscI AvrII BaeI BbeI BbvCI BfrBI BplI Bpu10I BsiWI BsmBI BstXI BstZ17I Bsu36I EcoICRI EcoNI EcoRV FseI FspAI KasI NarI NruI NsiI PacI PmlI PsrI RsrII SacI SacII SapI SexAI SfiI SfoI SrfI SwaI