(Circular) (Six-base) MAPSORT of: pFN21AE4528.seq Check: 1973 from: 1 to: 6436 pFN21AE4528 6436 bp With 182 enzymes: SgfI * March 28, 2012 14:09 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 4246 278 Size: 53 83 186 936 2710 2468 Fragments arranged by size: 2710 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2436 3779 1937 Size: 499 1343 4594 Fragments arranged by size: 4594 1343 499 Acc65I G'GTAC_C Cuts at: 2993 3757 2993 Size: 764 5672 AclI AA'CG_TT Cuts at: 1563 4563 4936 1563 Size: 3000 373 3063 Fragments arranged by size: 3063 3000 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 4499 5883 6151 1632 Size: 2867 1384 268 1917 Fragments arranged by size: 2867 1917 1384 268 AfeI AGC'GCT Cuts at: 2633 2633 Size: 6436 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 6239 AflIII A'CryG_T Cuts at: 1203 2439 2481 3405 5335 6312 1203 Size: 1236 42 924 1930 977 1327 Fragments arranged by size: 1930 1327 1236 977 924 42 AgeI A'CCGG_T Cuts at: 5267 5267 Size: 6436 AhdI GACnn_n'nnGTC Cuts at: 5165 5165 Size: 6436 AleI CACnn'nnGTG Cuts at: 1166 2480 1166 Size: 1314 5122 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 6436 AlwNI CAG_nnn'CTG Cuts at: 2344 4179 5751 2344 Size: 1835 1572 3029 Fragments arranged by size: 3029 1835 1572 ApaLI G'TGCA_C Cuts at: 4493 5649 4493 Size: 1156 5280 ApoI r'AATT_y Cuts at: 1493 3745 3971 4123 6232 1493 Size: 2252 226 152 2109 1697 Fragments arranged by size: 2252 2109 1697 226 152 AseI AT'TA_AT Cuts at: 160 1032 4990 160 Size: 872 3958 1606 Fragments arranged by size: 3958 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6436 AvaI C'yCGr_G Cuts at: 1870 1943 2775 3472 3761 1870 Size: 73 832 697 289 4545 Fragments arranged by size: 4545 832 697 289 73 BamHI G'GATC_C Cuts at: 2569 2805 3766 4133 2569 Size: 236 961 367 4872 Fragments arranged by size: 4872 961 367 236 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2131 2537 2993 3021 3183 Size: 325 217 627 344 406 456 28 162 Cuts at: 3183 3189 3467 3638 3757 5212 618 Size: 6 278 171 119 1455 1842 Fragments arranged by size: 1842 1455 627 456 406 344 325 278 217 171 162 119 28 6 BanII G_rGCy'C Cuts at: 1391 2751 3755 1391 Size: 1360 1004 4072 Fragments arranged by size: 4072 1360 1004 BbeI G_GCGC'C Cuts at: 2541 3642 2541 Size: 1101 5335 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2446 928 Size: 971 547 4918 Fragments arranged by size: 4918 971 547 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 4627 4661 1903 Size: 34 2690 34 3678 Fragments arranged by size: 3678 2690 34 34 BciVI GTATCCnnnnn_n' Cuts at: 2917 4328 5538 2917 Size: 1411 1210 3815 Fragments arranged by size: 3815 1411 1210 BclI T'GATC_A Cuts at: 1548 1548 Size: 6436 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2672 3338 3347 Size: 107 122 71 631 605 1000 666 9 Cuts at: 3347 3632 5047 136 Size: 285 1415 1525 Fragments arranged by size: 1525 1415 1000 666 631 605 285 122 107 71 9 BglII A'GATC_T Cuts at: 6431 6431 Size: 6436 BlpI GC'TnA_GC Cuts at: 3041 3119 3853 3041 Size: 78 734 5624 Fragments arranged by size: 5624 734 78 Bme1580I G_kGCm'C Cuts at: 1790 3470 4497 5653 1790 Size: 1680 1027 1156 2573 Fragments arranged by size: 2573 1680 1156 1027 BmgBI CAC'GTC Cuts at: 1318 2800 1318 Size: 1482 4954 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2866 3664 3688 Size: 416 53 83 399 24 1449 798 24 Cuts at: 3688 4167 5125 442 Size: 479 958 1753 Fragments arranged by size: 1753 1449 958 798 479 416 399 83 53 24 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6436 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3115 3115 Size: 6436 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2249 2765 3055 3607 5096 1239 Size: 1010 516 290 552 1489 2579 Fragments arranged by size: 2579 1489 1010 552 516 290 Bpu10I CC'TnA_GC Cuts at: 2840 2840 Size: 6436 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2984 4561 5426 5724 2984 Size: 1577 865 298 3696 Fragments arranged by size: 3696 1577 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 3226 5099 882 Size: 617 1727 1873 2219 Fragments arranged by size: 2219 1873 1727 617 BsaAI yAC'GTr Cuts at: 493 1204 2478 2482 2698 3646 4227 493 Size: 711 1274 4 216 948 581 2702 Fragments arranged by size: 2702 1274 948 711 581 216 4 BsaBI GATnn'nnATC Cuts at: 6430 6430 Size: 6436 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2348 2538 3095 3639 Size: 53 83 186 936 815 190 557 544 Cuts at: 3639 4243 4625 5260 275 Size: 604 382 635 1451 Fragments arranged by size: 1451 936 815 635 604 557 544 382 190 186 83 53 BsaWI w'CCGG_w Cuts at: 4869 5267 5541 5688 6099 4869 Size: 398 274 147 411 5206 Fragments arranged by size: 5206 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 6211 Fragments arranged by size: 6211 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 1186 Size: 6436 BseYI C'CCAG_C Cuts at: 2634 3462 5639 6135 2634 Size: 828 2177 496 2935 Fragments arranged by size: 2935 2177 828 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2720 3335 1163 Size: 573 984 615 4264 Fragments arranged by size: 4264 984 615 573 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2149 3886 4647 5675 1540 Size: 275 186 148 1737 761 1028 2301 Fragments arranged by size: 2301 1737 1028 761 275 186 148 BsiHKAI G_wGCw'C Cuts at: 2235 2751 4497 4582 5653 2235 Size: 516 1746 85 1071 3018 Fragments arranged by size: 3018 1746 1071 516 85 BsiWI C'GTAC_G Cuts at: 2478 2478 Size: 6436 BsmI GAATG_Cn' Cuts at: 3947 4040 3947 Size: 93 6343 BsmBI CGTCTCn'nnnn_ Cuts at: 3086 3086 Size: 6436 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2235 2751 3470 3755 4497 4582 5653 Size: 399 445 516 719 285 742 85 1071 Cuts at: 5653 1391 Size: 2174 Fragments arranged by size: 2174 1071 742 719 516 445 399 285 85 BspEI T'CCGG_A Cuts at: 6099 6099 Size: 6436 BspHI T'CATG_A Cuts at: 2106 4325 6055 2106 Size: 2219 1730 2487 Fragments arranged by size: 2487 2219 1730 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2340 2692 3791 844 Size: 1496 352 1099 3489 Fragments arranged by size: 3489 1496 1099 352 BsrBI CCG'CTC Cuts at: 1394 1823 2878 3591 4323 1394 Size: 429 1055 713 732 3507 Fragments arranged by size: 3507 1055 732 713 429 BsrDI GCAATG_nn' Cuts at: 65 1248 4931 5105 65 Size: 1183 3683 174 1396 Fragments arranged by size: 3683 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2235 3018 3186 3477 3635 3702 5080 Size: 110 425 783 168 291 158 67 1378 Cuts at: 5080 5267 1700 Size: 187 2869 Fragments arranged by size: 2869 1378 783 425 291 187 168 158 110 67 BsrGI T'GTAC_A Cuts at: 96 2658 3165 96 Size: 2562 507 3367 Fragments arranged by size: 3367 2562 507 BssHII G'CGCG_C Cuts at: 1925 2282 2374 2376 1925 Size: 357 92 2 5985 Fragments arranged by size: 5985 357 92 2 BssSI C'ACGA_G Cuts at: 2750 3569 4496 5508 2750 Size: 819 927 1012 3678 Fragments arranged by size: 3678 1012 927 819 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 6436 BstBI TT'CG_AA Cuts at: 5240 5240 Size: 6436 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 6436 BstXI CCAn_nnnn'nTGG Cuts at: 3433 3433 Size: 6436 BstYI r'GATC_y Cuts at: 1972 2569 2805 3766 4133 4518 4535 5987 6073 Size: 597 236 961 367 385 17 1452 86 Cuts at: 6073 6085 6431 1972 Size: 12 346 1977 Fragments arranged by size: 1977 1452 961 597 385 367 346 236 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2517 2733 3627 513 Size: 552 1452 216 894 3322 Fragments arranged by size: 3322 1452 894 552 216 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 2062 487 Size: 683 56 836 4861 Fragments arranged by size: 4861 836 683 56 BtsI GCAGTG_nn' Cuts at: 2293 2592 2738 3952 4746 4766 2293 Size: 299 146 1214 794 20 3963 Fragments arranged by size: 3963 1214 794 299 146 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6436 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 6401 DraI TTT'AAA Cuts at: 3740 3740 Size: 6436 DraIII CAC_nnn'GTG Cuts at: 2203 2203 Size: 6436 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 2828 3100 3136 5443 809 Size: 507 1512 272 36 2307 1802 Fragments arranged by size: 2307 1802 1512 507 272 36 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 3249 3330 3489 Size: 54 1425 50 275 18 1419 81 159 Cuts at: 3489 3624 3660 3883 4772 6401 8 Size: 135 36 223 889 1629 43 Fragments arranged by size: 1629 1425 1419 889 275 223 159 135 81 54 50 43 36 18 EagI C'GGCC_G Cuts at: 1537 1812 3883 1537 Size: 275 2071 4090 Fragments arranged by size: 4090 2071 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2222 4366 1353 Size: 869 2144 3423 Fragments arranged by size: 3423 2144 869 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2296 2341 2579 3496 3724 5023 Size: 1064 138 707 45 238 917 228 1299 Cuts at: 5023 5397 5543 387 Size: 374 146 1280 Fragments arranged by size: 1299 1280 1064 917 707 374 238 228 146 138 45 EcoICRI GAG'CTC Cuts at: 2749 2749 Size: 6436 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2249 2765 3055 3607 4499 5096 5883 Size: 393 617 516 290 552 892 597 787 Cuts at: 5883 6151 1239 Size: 268 1524 Fragments arranged by size: 1524 892 787 617 597 552 516 393 290 268 EcoO109I rG'GnC_Cy Cuts at: 2181 2320 2422 2467 2770 3075 3547 2181 Size: 139 102 45 303 305 472 5070 Fragments arranged by size: 5070 472 305 303 139 102 45 EcoRI G'AATT_C Cuts at: 3745 4123 3745 Size: 378 6058 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 3166 3358 3166 Size: 192 6244 FspI TGC'GCA Cuts at: 3371 4942 3371 Size: 1571 4865 HaeII r_GCGC'y Cuts at: 1678 2297 2541 2597 2635 2678 3293 3440 3524 Size: 619 244 56 38 43 615 147 84 Cuts at: 3524 3533 3642 5583 1678 Size: 9 109 1941 2531 Fragments arranged by size: 2531 1941 619 615 244 147 109 84 56 43 38 9 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 5077 5109 5151 5183 1373 Size: 32 135 32 3505 32 42 32 2626 Fragments arranged by size: 3505 2626 135 42 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2437 3669 3780 4026 669 Size: 988 281 499 1232 111 246 3079 Fragments arranged by size: 3079 1232 988 499 281 246 111 HindIII A'AGCT_T Cuts at: 748 748 Size: 6436 HpaI GTT'AAC Cuts at: 4026 4026 Size: 6436 KasI G'GCGC_C Cuts at: 2537 3638 2537 Size: 1101 5335 KpnI G_GTAC'C Cuts at: 2997 3761 2997 Size: 764 5672 MfeI C'AATT_G Cuts at: 4035 4035 Size: 6436 MluI A'CGCG_T Cuts at: 6312 6312 Size: 6436 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2706 5550 5734 1305 Size: 1401 2844 184 2007 Fragments arranged by size: 2844 2007 1401 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 3332 3662 10 Size: 54 1425 343 1500 330 2784 Fragments arranged by size: 2784 1500 1425 343 330 54 MslI CAynn'nnrTG Cuts at: 518 1166 2399 2480 4394 4753 4912 518 Size: 648 1233 81 1914 359 159 2042 Fragments arranged by size: 2042 1914 1233 648 359 159 81 MspA1I CmG'CkG Cuts at: 1606 2046 2137 2266 2519 2919 3081 3629 3852 Size: 440 91 129 253 400 162 548 223 Cuts at: 3852 4529 5677 5922 1606 Size: 677 1148 245 2120 Fragments arranged by size: 2120 1148 677 548 440 400 253 245 223 162 129 91 NaeI GCC'GGC Cuts at: 3479 3637 3704 3479 Size: 158 67 6211 Fragments arranged by size: 6211 158 67 NarI GG'CG_CC Cuts at: 2538 3639 2538 Size: 1101 5335 NcoI C'CATG_G Cuts at: 513 1065 2733 513 Size: 552 1668 4216 Fragments arranged by size: 4216 1668 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 6436 NgoMIV G'CCGG_C Cuts at: 3477 3635 3702 3477 Size: 158 67 6211 Fragments arranged by size: 6211 158 67 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6436 NotI GC'GGCC_GC Cuts at: 3883 3883 Size: 6436 NspI r_CATG'y Cuts at: 1132 1229 2443 3040 3409 3794 5339 1132 Size: 97 1214 597 369 385 1545 2229 Fragments arranged by size: 2229 1545 1214 597 385 369 97 PasI CC'CwG_GG Cuts at: 1348 2508 1348 Size: 1160 5276 PciI A'CATG_T Cuts at: 2439 3405 5335 2439 Size: 966 1930 3540 Fragments arranged by size: 3540 1930 966 PflMI CCAn_nnn'nTGG Cuts at: 1108 2344 1108 Size: 1236 5200 PmeI GTTT'AAAC Cuts at: 3740 3740 Size: 6436 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4505 4537 6018 6050 4505 Size: 32 1481 32 4891 Fragments arranged by size: 4891 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2320 2422 2467 2770 3075 2320 Size: 102 45 303 305 5681 Fragments arranged by size: 5681 305 303 102 45 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 6436 PsiI TTA'TAA Cuts at: 4006 4006 Size: 6436 PstI C_TGCA'G Cuts at: 830 2038 3788 830 Size: 1208 1750 3478 Fragments arranged by size: 3478 1750 1208 PvuI CG_AT'CG Cuts at: 2001 2149 2001 Size: 148 6288 RsrII CG'GwC_CG Cuts at: 3711 3711 Size: 6436 SacI G_AGCT'C Cuts at: 2751 2751 Size: 6436 SacII CC_GC'GG Cuts at: 2520 3630 2520 Size: 1110 5326 SalI G'TCGA_C Cuts at: 1936 2435 3778 1936 Size: 499 1343 4594 Fragments arranged by size: 4594 1343 499 SbfI CC_TGCA'GG Cuts at: 3788 3788 Size: 6436 ScaI AGT'ACT Cuts at: 4684 6396 4684 Size: 1712 4724 SexAI A'CCwGG_T Cuts at: 2199 2199 Size: 6436 SfcI C'TryA_G Cuts at: 826 1045 2034 3784 4919 5600 5791 826 Size: 219 989 1750 1135 681 191 1471 Fragments arranged by size: 1750 1471 1135 989 681 219 191 SfiI GGCCn_nnn'nGGCC Cuts at: 3338 3347 3632 3338 Size: 9 285 6142 Fragments arranged by size: 6142 285 9 SfoI GGC'GCC Cuts at: 2539 3640 2539 Size: 1101 5335 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6436 SgrAI Cr'CCGG_yG Cuts at: 1700 2235 1700 Size: 535 5901 SmaI CCC'GGG Cuts at: 1872 2777 3763 1872 Size: 905 986 4545 Fragments arranged by size: 4545 986 905 SmlI C'TyrA_G Cuts at: 820 1017 1943 2963 4540 5441 5703 820 Size: 197 926 1020 1577 901 262 1553 Fragments arranged by size: 1577 1553 1020 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 4227 493 Size: 3734 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 6436 SphI G_CATG'C Cuts at: 1132 3040 3794 1132 Size: 1908 754 3774 Fragments arranged by size: 3774 1908 754 SspI AAT'ATT Cuts at: 5 52 4360 5 Size: 47 4308 2081 Fragments arranged by size: 4308 2081 47 StuI AGG'CCT Cuts at: 1517 2931 2969 3614 1517 Size: 1414 38 645 4339 Fragments arranged by size: 4339 1414 645 38 StyI C'CwwG_G Cuts at: 513 1065 2733 3284 3333 3875 513 Size: 552 1668 551 49 542 3074 Fragments arranged by size: 3074 1668 552 551 542 49 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 3289 3646 3670 4463 4480 4633 4818 Size: 1255 1709 357 24 793 17 153 185 Cuts at: 4818 5263 325 Size: 445 1498 Fragments arranged by size: 1709 1498 1255 793 445 357 185 153 24 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2658 3002 3063 Size: 274 80 33 51 1443 681 344 61 Cuts at: 3063 3165 3675 4682 6394 96 Size: 102 510 1007 1712 138 Fragments arranged by size: 1712 1443 1007 681 510 344 274 138 102 80 61 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2891 2923 6396 6428 2891 Size: 32 3473 32 2899 Fragments arranged by size: 3473 2899 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 6436 XbaI T'CTAG_A Cuts at: 3772 3772 Size: 6436 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2740 3005 2740 Size: 265 6171 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 6436 XmaI C'CCGG_G Cuts at: 1870 2775 3761 1870 Size: 905 986 4545 Fragments arranged by size: 4545 986 905 XmnI GAAnn'nnTTC Cuts at: 4565 4565 Size: 6436 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 4244 276 Size: 53 83 186 936 2710 2468 Fragments arranged by size: 2710 2468 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AfeI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsiWI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NarI NcoI NdeI NgoMIV NheI NotI NspI PasI PciI PflMI PmeI PpiI PpuMI PshAI PsiI PstI PvuI RsrII SacI SacII SalI SbfI ScaI SexAI SfcI SfiI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AloI ApaI AscI AvrII BaeI BbvCI BfrBI BstZ17I Bsu36I EcoNI EcoRV FseI FspAI NruI NsiI PacI PfoI PmlI PspOMI PspXI PsrI PvuII SanDI SapI SrfI SwaI