(Linear) (Six-base) MAPSORT of: KIEE4528.seq Check: 975 from: 1 to: 1731 KIEE4528.orf With 182 enzymes: SgfI * March 22, 2012 18:17 .. AccI GT'mk_AC Cuts at: 0 431 1731 Size: 431 1300 Acc65I G'GTAC_C Cuts at: 0 988 1731 Size: 988 743 AfeI AGC'GCT Cuts at: 0 628 1731 Size: 628 1103 AflIII A'CryG_T Cuts at: 0 434 476 1400 1731 Size: 434 42 924 331 Fragments arranged by size: 924 434 331 42 AleI CACnn'nnGTG Cuts at: 0 475 1731 Size: 475 1256 AlwNI CAG_nnn'CTG Cuts at: 0 339 1731 Size: 339 1392 AvaI C'yCGr_G Cuts at: 0 770 1467 1731 Size: 770 697 264 Fragments arranged by size: 770 697 264 BamHI G'GATC_C Cuts at: 0 564 800 1731 Size: 564 236 931 Fragments arranged by size: 931 564 236 BanI G'GyrC_C Cuts at: 0 126 532 988 1016 1178 1184 1462 1633 Size: 126 406 456 28 162 6 278 171 Cuts at: 1633 1731 Size: 98 Fragments arranged by size: 456 406 278 171 162 126 98 28 6 BanII G_rGCy'C Cuts at: 0 746 1731 Size: 746 985 BbeI G_GCGC'C Cuts at: 0 536 1637 1731 Size: 536 1101 94 Fragments arranged by size: 1101 536 94 BbsI GAAGACnn'nnnn_ Cuts at: 0 441 1731 Size: 441 1290 BciVI GTATCCnnnnn_n' Cuts at: 0 912 1731 Size: 912 819 BglI GCCn_nnn'nGGC Cuts at: 0 667 1333 1342 1627 1731 Size: 667 666 9 285 104 Fragments arranged by size: 667 666 285 104 9 BlpI GC'TnA_GC Cuts at: 0 1036 1114 1731 Size: 1036 78 617 Fragments arranged by size: 1036 617 78 Bme1580I G_kGCm'C Cuts at: 0 1465 1731 Size: 1465 266 BmgBI CAC'GTC Cuts at: 0 795 1731 Size: 795 936 BmrI ACTGGGnnnn_n' Cuts at: 0 861 1659 1683 1731 Size: 861 798 24 48 Fragments arranged by size: 861 798 48 24 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 0 1110 1731 Size: 1110 621 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 244 760 1050 1602 1731 Size: 244 516 290 552 129 Fragments arranged by size: 552 516 290 244 129 Bpu10I CC'TnA_GC Cuts at: 0 835 1731 Size: 835 896 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 979 1731 Size: 979 752 BsaI GGTCTCn'nnnn_ Cuts at: 0 1221 1731 Size: 1221 510 BsaAI yAC'GTr Cuts at: 0 473 477 693 1641 1731 Size: 473 4 216 948 90 Fragments arranged by size: 948 473 216 90 4 BsaHI Gr'CG_yC Cuts at: 0 343 533 1090 1634 1731 Size: 343 190 557 544 97 Fragments arranged by size: 557 544 343 190 97 BseYI C'CCAG_C Cuts at: 0 629 1457 1731 Size: 629 828 274 Fragments arranged by size: 828 629 274 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 0 715 1330 1731 Size: 715 615 401 Fragments arranged by size: 715 615 401 BsiEI CG_ry'CG Cuts at: 0 144 1731 Size: 144 1587 BsiHKAI G_wGCw'C Cuts at: 0 230 746 1731 Size: 230 516 985 Fragments arranged by size: 985 516 230 BsiWI C'GTAC_G Cuts at: 0 473 1731 Size: 473 1258 BsmBI CGTCTCn'nnnn_ Cuts at: 0 1081 1731 Size: 1081 650 Bsp1286I G_dGCh'C Cuts at: 0 230 746 1465 1731 Size: 230 516 719 266 Fragments arranged by size: 719 516 266 230 BspHI T'CATG_A Cuts at: 0 101 1731 Size: 101 1630 BspMI ACCTGCnnnn'nnnn_ Cuts at: 0 335 687 1731 Size: 335 352 1044 Fragments arranged by size: 1044 352 335 BsrBI CCG'CTC Cuts at: 0 873 1586 1731 Size: 873 713 145 Fragments arranged by size: 873 713 145 BsrFI r'CCGG_y Cuts at: 0 230 1013 1181 1472 1630 1697 1731 Size: 230 783 168 291 158 67 34 Fragments arranged by size: 783 291 230 168 158 67 34 BsrGI T'GTAC_A Cuts at: 0 653 1160 1731 Size: 653 507 571 Fragments arranged by size: 653 571 507 BssHII G'CGCG_C Cuts at: 0 277 369 371 1731 Size: 277 92 2 1360 Fragments arranged by size: 1360 277 92 2 BssSI C'ACGA_G Cuts at: 0 745 1564 1731 Size: 745 819 167 Fragments arranged by size: 819 745 167 BstXI CCAn_nnnn'nTGG Cuts at: 0 1428 1731 Size: 1428 303 BstYI r'GATC_y Cuts at: 0 564 800 1731 Size: 564 236 931 Fragments arranged by size: 931 564 236 BtgI C'CryG_G Cuts at: 0 512 728 1622 1731 Size: 512 216 894 109 Fragments arranged by size: 894 512 216 109 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 0 57 1731 Size: 57 1674 BtsI GCAGTG_nn' Cuts at: 0 288 587 733 1731 Size: 288 299 146 998 Fragments arranged by size: 998 299 288 146 DraIII CAC_nnn'GTG Cuts at: 0 198 1731 Size: 198 1533 DrdI GACnn_nn'nnGTC Cuts at: 0 823 1095 1131 1731 Size: 823 272 36 600 Fragments arranged by size: 823 600 272 36 EaeI y'GGCC_r Cuts at: 0 1244 1325 1484 1619 1655 1731 Size: 1244 81 159 135 36 76 Fragments arranged by size: 1244 159 135 81 76 36 EarI CTCTTCn'nnn_ Cuts at: 0 217 1731 Size: 217 1514 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 0 291 336 574 1491 1719 1731 Size: 291 45 238 917 228 12 Fragments arranged by size: 917 291 238 228 45 12 EcoICRI GAG'CTC Cuts at: 0 744 1731 Size: 744 987 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 0 244 760 1050 1602 1731 Size: 244 516 290 552 129 Fragments arranged by size: 552 516 290 244 129 EcoO109I rG'GnC_Cy Cuts at: 0 176 315 417 462 765 1070 1542 1731 Size: 176 139 102 45 303 305 472 189 Fragments arranged by size: 472 305 303 189 176 139 102 45 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 0 1161 1353 1731 Size: 1161 192 378 Fragments arranged by size: 1161 378 192 FspI TGC'GCA Cuts at: 0 1366 1731 Size: 1366 365 HaeII r_GCGC'y Cuts at: 0 292 536 592 630 673 1288 1435 1519 Size: 292 244 56 38 43 615 147 84 Cuts at: 1519 1528 1637 1731 Size: 9 109 94 Fragments arranged by size: 615 292 244 147 109 94 84 56 43 38 9 HincII GTy'rAC Cuts at: 0 432 1664 1731 Size: 432 1232 67 Fragments arranged by size: 1232 432 67 KasI G'GCGC_C Cuts at: 0 532 1633 1731 Size: 532 1101 98 Fragments arranged by size: 1101 532 98 KpnI G_GTAC'C Cuts at: 0 992 1731 Size: 992 739 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 0 701 1731 Size: 701 1030 MscI TGG'CCA Cuts at: 0 1327 1657 1731 Size: 1327 330 74 Fragments arranged by size: 1327 330 74 MslI CAynn'nnrTG Cuts at: 0 394 475 1731 Size: 394 81 1256 Fragments arranged by size: 1256 394 81 MspA1I CmG'CkG Cuts at: 0 41 132 261 514 914 1076 1624 1731 Size: 41 91 129 253 400 162 548 107 Fragments arranged by size: 548 400 253 162 129 107 91 41 NaeI GCC'GGC Cuts at: 0 1474 1632 1699 1731 Size: 1474 158 67 32 Fragments arranged by size: 1474 158 67 32 NarI GG'CG_CC Cuts at: 0 533 1634 1731 Size: 533 1101 97 Fragments arranged by size: 1101 533 97 NcoI C'CATG_G Cuts at: 0 728 1731 Size: 728 1003 NgoMIV G'CCGG_C Cuts at: 0 1472 1630 1697 1731 Size: 1472 158 67 34 Fragments arranged by size: 1472 158 67 34 NspI r_CATG'y Cuts at: 0 438 1035 1404 1731 Size: 438 597 369 327 Fragments arranged by size: 597 438 369 327 PasI CC'CwG_GG Cuts at: 0 503 1731 Size: 503 1228 PciI A'CATG_T Cuts at: 0 434 1400 1731 Size: 434 966 331 Fragments arranged by size: 966 434 331 PflMI CCAn_nnn'nTGG Cuts at: 0 339 1731 Size: 339 1392 PpuMI rG'GwC_Cy Cuts at: 0 315 417 462 765 1070 1731 Size: 315 102 45 303 305 661 Fragments arranged by size: 661 315 305 303 102 45 PstI C_TGCA'G Cuts at: 0 33 1731 Size: 33 1698 PvuI CG_AT'CG Cuts at: 0 144 1731 Size: 144 1587 RsrII CG'GwC_CG Cuts at: 0 1706 1731 Size: 1706 25 SacI G_AGCT'C Cuts at: 0 746 1731 Size: 746 985 SacII CC_GC'GG Cuts at: 0 515 1625 1731 Size: 515 1110 106 Fragments arranged by size: 1110 515 106 SalI G'TCGA_C Cuts at: 0 430 1731 Size: 430 1301 SexAI A'CCwGG_T Cuts at: 0 194 1731 Size: 194 1537 SfcI C'TryA_G Cuts at: 0 29 1731 Size: 29 1702 SfiI GGCCn_nnn'nGGCC Cuts at: 0 1333 1342 1627 1731 Size: 1333 9 285 104 Fragments arranged by size: 1333 285 104 9 SfoI GGC'GCC Cuts at: 0 534 1635 1731 Size: 534 1101 96 Fragments arranged by size: 1101 534 96 SgrAI Cr'CCGG_yG Cuts at: 0 230 1731 Size: 230 1501 SmaI CCC'GGG Cuts at: 0 772 1731 Size: 772 959 SmlI C'TyrA_G Cuts at: 0 958 1731 Size: 958 773 SphI G_CATG'C Cuts at: 0 1035 1731 Size: 1035 696 StuI AGG'CCT Cuts at: 0 926 964 1609 1731 Size: 926 38 645 122 Fragments arranged by size: 926 645 122 38 StyI C'CwwG_G Cuts at: 0 728 1279 1328 1731 Size: 728 551 49 403 Fragments arranged by size: 728 551 403 49 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 0 1284 1641 1665 1731 Size: 1284 357 24 66 Fragments arranged by size: 1284 357 66 24 TatI w'GTAC_w Cuts at: 0 653 997 1058 1160 1670 1731 Size: 653 344 61 102 510 61 Fragments arranged by size: 653 510 344 102 61 61 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 0 886 918 1731 Size: 886 32 813 Fragments arranged by size: 886 813 32 XcmI CCAnnnn_n'nnnnTGG Cuts at: 0 735 1000 1731 Size: 735 265 731 Fragments arranged by size: 735 731 265 XmaI C'CCGG_G Cuts at: 0 770 1731 Size: 770 961 Enzymes that do cut: AccI Acc65I AfeI AflIII AleI AlwNI AvaI BamHI BanI BanII BbeI BbsI BciVI BglI BlpI Bme1580I BmgBI BmrI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaHI BseYI BsgI BsiEI BsiHKAI BsiWI BsmBI Bsp1286I BspHI BspMI BsrBI BsrFI BsrGI BssHII BssSI BstXI BstYI BtgI BtgZI BtsI DraIII DrdI EaeI EarI EciI EcoICRI Eco57MI EcoO109I FalI FspI HaeII HincII KasI KpnI MmeI MscI MslI MspA1I NaeI NarI NcoI NgoMIV NspI PasI PciI PflMI PpuMI PstI PvuI RsrII SacI SacII SalI SexAI SfcI SfiI SfoI SgrAI SmaI SmlI SphI StuI StyI TaqII TatI TstI XcmI XmaI Enzymes that do not cut: AarI AatII AclI AcuI AflII AgeI AhdI AlfI AloI ApaI ApaLI ApoI AscI AseI AsiSI AvrII BaeI BbvCI BcgI BclI BfrBI BglII BmtI BsaBI BsaWI BsaXI BseRI BsmI BspEI BsrDI BstAPI BstBI BstEII BstZ17I Bsu36I ClaI CspCI DraI EagI EcoNI EcoRI EcoRV FseI FspAI Hin4I HindIII HpaI MfeI MluI NdeI NheI NotI NruI NsiI PacI PfoI PmeI PmlI PpiI PshAI PsiI PspOMI PspXI PsrI PvuII SanDI SapI SbfI ScaI SgfI SnaBI SpeI SrfI SspI SwaI Tth111I XbaI XhoI XmnI ZraI