################################## # NO CUT ENZYMES ( 126 enzymes) # ################################## AatII AccII AccIII AcyI AflII AflIII AgeI AhdI Alw44I AocI Aor51HI ApaLI AscI AseI AsnI Asp700I AspEI AviII AvrII BalI BamHI BbeI BbrPI BcgI BciVI BclI BfrI BglI BglII BlnI BsaAI BsaBI BsaHI BsaWI BseAI BsiEI BsiWI BsmBI Bsp1407I BspDI BspEI BspHI BspLU11I BsrFI BsrGI BssHII Bst1107I BstAPI BstBI BstEII BstPI BstUI BstYI BstZ17I Bsu36I Cfr10I ClaI CpoI DraI EaeI EagI Eam1105I EclXI Eco47III Eco52I Eco81I EcoO65I EcoRV EcoT22I FbaI FseI FspI HaeII HgaI Hin1I KasI KspI MamI MfeI MflI MluI MluNI MroI MscI MunI MvnI NaeI NarI NdeI NgoMIV NotI NruI NsiI NspV PacI PaeR7I PinAI PmaCI PmeI PmlI PshBI PvuI RcaI RsrII SacII SalI SapI ScaI SexAI SfiI SfoI SfuI SgrAI SmaI SnaBI SpeI SphI SspBI SspI SwaI XbaI XhoI XhoII XmaCI XmaI XmnI ################################### # ONE CUT ENZYMES ( 48 enzymes) # ################################### Acc65I 935 GGTACC G^GTACC AccI 911 GTAGAC GT^[AC][GT]AC AclI 780 AACGTT AA^CGTT AcsI 64 GAATTC [GA]^AATT[CT] ApaI 953 GGGCCC GGGCC^C ApoI 64 GAATTC [GA]^AATT[CT] Asp718I 935 GGTACC G^GTACC AspHI 682 GAGCTC G[AT]GC[AT]^C AspI 304 GACCTTGTC GAC[ACGT]^[ACGT][ACGT]GTC AvaI 1187 CCCGAG C^[CT]CG[GA]G BlpI 802 GCTGAGC GC^T[ACGT]AGC Bpu1102I 802 GCTGAGC GC^T[ACGT]AGC BsiHKAI 682 GAGCTC G[AT]GC[AT]^C BsmI 252 GAATGC GAATGC(1/-1) BsoBI 1187 CCCGAG C^[CT]CG[GA]G BssSI 1085 CACGAG CACGAG(-5/-1) BstXI 185 CCAACAAAATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG CelII 802 GCTGAGC GC^T[ACGT]AGC CfoI 295 GCGC GCG^C DraIII 244 CACCCTGTG CAC[ACGT][ACGT][ACGT]^GTG DrdI 204 GACTAGCAAGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC DsaI 942 CCATGG C^C[GA][CT]GG EcoNI 144 CCTTGTGGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG EcoRI 64 GAATTC G^AATTC EcoT14I 942 CCATGG C^C[AT][AT]GG HhaI 295 GCGC GCG^C HinP1I 295 GCGC G^CGC HindIII 160 AAGCTT A^AGCTT KpnI 935 GGTACC GGTAC^C MslI 141 CATCCTTGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG NcoI 942 CCATGG C^CATGG NheI 807 GCTAGC G^CTAGC NspI 1494 ACATGC [GA]CATG^[CT] PflFI 304 GACCTTGTC GAC[ACGT]^[ACGT][ACGT]GTC PflMI 1535 CCAATAAGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG PpuMI 302 AGGACCT [GA]G^G[AT]CC[CT] PshAI 1110 GACAGGAGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC Psp1406I 780 AACGTT AA^CGTT SacI 682 GAGCTC GAGCT^C SbfI 312 CCTGCAGG CCTGCA^GG SmlI 1569 CTTGAG C^T[CT][GA]AG Sse8387I 312 CCTGCAGG CCTGCA^GG StuI 835 AGGCCT AGG^CCT StyI 942 CCATGG C^C[AT][AT]GG TfiI 735 GAATC G^A[AT]TC Tth111I 304 GACCTTGTC GAC[ACGT]^[ACGT][ACGT]GTC Van91I 1535 CCAATAAGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG XcmI 717 CCACGAACGAGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG ################################### # TWO CUT ENZYMES ( 24 enzymes) # ################################### AvaII 303 GGACC G^G[AT]CC 435 GGACC BanI 935 GGTACC G^G[CT][GA]CC 1387 GGTGCC BanII 682 GAGCTC G[GA]GC[CT]^C 953 GGGCCC BbsI 649 GTCTTC GAAGAC(2/6) 1396 GTCTTC BbvCI 636 CCTCAGC CCTCAGC(-5/-2) 226 GCTGAGG BcnI 593 CCGGG CC^[GC]GG 1137 CCGGG BpuAI 649 GTCTTC GAAGAC(2/6) 1396 GTCTTC BsaI 1572 GAGACC GGTCTC(1/5) 1530 GGTCTC BseRI 598 GAGGAG GAGGAG(10/8) 985 GAGGAG BsmFI 539 GGGAC GGGAC(10/14) 998 GGGAC BsrBI 519 CCGCTC CCGCTC(-3/-3) 1467 GAGCGG EarI 766 GAAGAG CTCTTC(1/4) 918 GAAGAG HapII 593 CCGG C^CGG 1137 CCGG HpaI 783 GTTAAC GTT^AAC 1221 GTTAAC HpaII 593 CCGG C^CGG 1137 CCGG Ksp632I 766 GAAGAG CTCTTC(1/4) 918 GAAGAG MaeII 781 ACGT A^CGT 1351 ACGT MspI 593 CCGG C^CGG 1137 CCGG NciI 593 CCGGG CC^[GC]GG 1137 CCGGG PleI 319 GACTC GAGTC(4/5) 1115 GAGTC TaqI 1049 TCGA T^CGA 1292 TCGA Tsp45I 618 GTCAC ^GT[GC]AC 1478 GTGAC TspRI 372 CACTG CA[GC]TG[ACGT][ACGT]^ 1122 CACTG TthHB8I 1049 TCGA T^CGA 1292 TCGA ######################################### # 3 OR MORE CUT ENZYMES ( 60 enzymes) # ######################################### AciI AfaI AluI AlwI AlwNI BbvI BfaI BmyI BpmI BsaJI BsgI BsiYI BslI BsmAI Bsp1286I BspMI BsrDI BsrI BssKI BstF5I BstNI Cac8I Cfr13I DdeI DpnII DraII EcoO109I EcoRII FauI Fnu4HI FokI HaeIII HincII HindII HinfI HphI ItaI MaeI MaeIII MboI MboII MnlI MseI MspA1I MvaI MwoI NdeII NlaIII NlaIV PstI PvuII RsaI Sau3AI Sau96I ScrFI SfaNI SfcI Tru9I TseI Tsp509I