(Circular) (Six-base) MAPSORT of: pFN21AE6408.seq Check: 4723 from: 1 to: 6304 pFN21AE6408 6304 bp With 182 enzymes: SgfI * March 18, 2015 14:51 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 2566 2566 Size: 6304 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 4114 278 Size: 53 83 186 936 2578 2468 Fragments arranged by size: 2578 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2917 3647 1937 Size: 980 730 4594 Fragments arranged by size: 4594 980 730 Acc65I G'GTAC_C Cuts at: 2940 3625 2940 Size: 685 5619 AclI AA'CG_TT Cuts at: 1563 2786 4431 4804 1563 Size: 1223 1645 373 3063 Fragments arranged by size: 3063 1645 1223 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2405 4367 5751 6019 1632 Size: 773 1962 1384 268 1917 Fragments arranged by size: 1962 1917 1384 773 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 6107 AflIII A'CryG_T Cuts at: 1203 5203 6180 1203 Size: 4000 977 1327 Fragments arranged by size: 4000 1327 977 AgeI A'CCGG_T Cuts at: 5135 5135 Size: 6304 AhdI GACnn_n'nnGTC Cuts at: 5033 5033 Size: 6304 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 6304 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 2610 1184 Size: 1426 4878 AlwNI CAG_nnn'CTG Cuts at: 2134 2225 2632 2807 4047 5619 2134 Size: 91 407 175 1240 1572 2819 Fragments arranged by size: 2819 1572 1240 407 175 91 ApaI G_GGCC'C Cuts at: 2962 2962 Size: 6304 ApaLI G'TGCA_C Cuts at: 4361 5517 4361 Size: 1156 5148 ApoI r'AATT_y Cuts at: 1493 2069 3613 3839 3991 6100 1493 Size: 576 1544 226 152 2109 1697 Fragments arranged by size: 2109 1697 1544 576 226 152 AseI AT'TA_AT Cuts at: 160 1032 4858 160 Size: 872 3826 1606 Fragments arranged by size: 3826 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6304 AvaI C'yCGr_G Cuts at: 1870 1943 3192 3629 1870 Size: 73 1249 437 4545 Fragments arranged by size: 4545 1249 437 73 BaeI ACnnnnGTAyCnnnnnnn_nnnnn' Cuts at: 3432 3465 3432 Size: 33 6271 BamHI G'GATC_C Cuts at: 3634 4001 3634 Size: 367 5937 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2940 3392 3625 5080 618 Size: 325 217 627 1153 452 233 1455 1842 Fragments arranged by size: 1842 1455 1153 627 452 325 233 217 BanII G_rGCy'C Cuts at: 1391 2691 2962 3623 1391 Size: 1300 271 661 4072 Fragments arranged by size: 4072 1300 661 271 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2647 3394 928 Size: 971 748 747 3838 Fragments arranged by size: 3838 971 748 747 BbvCI CC'TCA_GC Cuts at: 2232 2642 2232 Size: 410 5894 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 4495 4529 1903 Size: 34 2558 34 3678 Fragments arranged by size: 3678 2558 34 34 BciVI GTATCCnnnnn_n' Cuts at: 4196 5406 4196 Size: 1210 5094 BclI T'GATC_A Cuts at: 1548 1548 Size: 6304 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 4915 136 Size: 107 122 71 631 605 3243 1525 Fragments arranged by size: 3243 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 6299 6299 Size: 6304 BlpI GC'TnA_GC Cuts at: 2808 3721 2808 Size: 913 5391 Bme1580I G_kGCm'C Cuts at: 1790 2091 2683 2962 4365 5521 1790 Size: 301 592 279 1403 1156 2573 Fragments arranged by size: 2573 1403 1156 592 301 279 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 6304 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 4035 4993 442 Size: 416 53 83 399 24 2618 958 1753 Fragments arranged by size: 2618 1753 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 2816 1056 Size: 1760 4544 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2324 2705 2754 4964 1239 Size: 1085 381 49 2210 2579 Fragments arranged by size: 2579 2210 1085 381 49 Bpu10I CC'TnA_GC Cuts at: 2051 2232 2642 2051 Size: 181 410 5713 Fragments arranged by size: 5713 410 181 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 3595 4429 5294 5592 3595 Size: 834 865 298 4307 Fragments arranged by size: 4307 865 834 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 3541 3571 4967 882 Size: 617 2042 30 1396 2219 Fragments arranged by size: 2219 2042 1396 617 30 BsaAI yAC'GTr Cuts at: 493 1204 4095 493 Size: 711 2891 2702 Fragments arranged by size: 2891 2702 711 BsaBI GATnn'nnATC Cuts at: 6298 6298 Size: 6304 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 4111 4493 5128 275 Size: 53 83 186 936 2578 382 635 1451 Fragments arranged by size: 2578 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 4737 5135 5409 5556 5967 4737 Size: 398 274 147 411 5074 Fragments arranged by size: 5074 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2984 3014 1178 Size: 30 165 30 1581 30 4468 Fragments arranged by size: 4468 1581 165 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2618 3005 1186 Size: 1432 387 4485 Fragments arranged by size: 4485 1432 387 BseYI C'CCAG_C Cuts at: 2184 5507 6003 2184 Size: 3323 496 2485 Fragments arranged by size: 3323 2485 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2081 2147 2378 2657 1163 Size: 573 345 66 231 279 4810 Fragments arranged by size: 4810 573 345 279 231 66 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3754 4515 5543 1540 Size: 275 186 1753 761 1028 2301 Fragments arranged by size: 2301 1753 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2691 4365 4450 5521 2691 Size: 1674 85 1071 3474 Fragments arranged by size: 3474 1674 1071 85 BsmI GAATG_Cn' Cuts at: 2263 3815 3908 2263 Size: 1552 93 4659 Fragments arranged by size: 4659 1552 93 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2091 2683 2691 2962 3623 4365 4450 Size: 399 301 592 8 271 661 742 85 Cuts at: 4450 5521 1391 Size: 1071 2174 Fragments arranged by size: 2174 1071 742 661 592 399 301 271 85 8 BspEI T'CCGG_A Cuts at: 5967 5967 Size: 6304 BspHI T'CATG_A Cuts at: 4193 5923 4193 Size: 1730 4574 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2324 2566 2862 3659 844 Size: 1480 242 296 797 3489 Fragments arranged by size: 3489 1480 797 296 242 BsrBI CCG'CTC Cuts at: 1394 1823 2526 3474 4191 1394 Size: 429 703 948 717 3507 Fragments arranged by size: 3507 948 717 703 429 BsrDI GCAATG_nn' Cuts at: 65 1248 2024 2496 2973 4799 4973 65 Size: 1183 776 472 477 1826 174 1396 Fragments arranged by size: 1826 1396 1183 776 477 472 174 BsrFI r'CCGG_y Cuts at: 1700 1810 4948 5135 1700 Size: 110 3138 187 2869 Fragments arranged by size: 3138 2869 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 6304 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6304 BssSI C'ACGA_G Cuts at: 3090 4364 5376 3090 Size: 1274 1012 4018 Fragments arranged by size: 4018 1274 1012 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 6304 BstBI TT'CG_AA Cuts at: 5108 5108 Size: 6304 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 6304 BstXI CCAn_nnnn'nTGG Cuts at: 2197 2197 Size: 6304 BstYI r'GATC_y Cuts at: 1972 3634 4001 4386 4403 5855 5941 5953 6299 Size: 1662 367 385 17 1452 86 12 346 Cuts at: 6299 1972 Size: 1977 Fragments arranged by size: 1977 1662 1452 385 367 346 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2947 513 Size: 552 1882 3870 Fragments arranged by size: 3870 1882 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5565 Fragments arranged by size: 5565 683 56 BtsI GCAGTG_nn' Cuts at: 3820 4614 4634 3820 Size: 794 20 5490 Fragments arranged by size: 5490 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6304 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 6269 DraI TTT'AAA Cuts at: 3608 3608 Size: 6304 DraIII CAC_nnn'GTG Cuts at: 2254 2254 Size: 6304 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 2215 5311 809 Size: 507 899 3096 1802 Fragments arranged by size: 3096 1802 899 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 3751 4640 6269 Size: 54 1425 50 275 18 1921 889 1629 Cuts at: 6269 8 Size: 43 Fragments arranged by size: 1921 1629 1425 889 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3751 1537 Size: 275 1939 4090 Fragments arranged by size: 4090 1939 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2766 2918 4234 1353 Size: 1413 152 1316 3423 Fragments arranged by size: 3423 1413 1316 152 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4891 5265 5411 387 Size: 1064 138 3302 374 146 1280 Fragments arranged by size: 3302 1280 1064 374 146 138 EcoICRI GAG'CTC Cuts at: 2689 2689 Size: 6304 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2324 2405 2705 2754 4367 4964 5751 Size: 393 692 81 300 49 1613 597 787 Cuts at: 5751 6019 1239 Size: 268 1524 Fragments arranged by size: 1613 1524 787 692 597 393 300 268 81 49 EcoNI CCTnn'n_nnAGG Cuts at: 2153 2153 Size: 6304 EcoO109I rG'GnC_Cy Cuts at: 2308 2958 2959 2308 Size: 650 1 5653 Fragments arranged by size: 5653 650 1 EcoRI G'AATT_C Cuts at: 2069 3613 3991 2069 Size: 1544 378 4382 Fragments arranged by size: 4382 1544 378 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2183 2183 Size: 6304 FspI TGC'GCA Cuts at: 4810 4810 Size: 6304 HaeII r_GCGC'y Cuts at: 1678 5451 1678 Size: 3773 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2869 2901 3023 3055 4945 Size: 32 135 32 1297 32 122 32 1890 Cuts at: 4945 4977 5019 5051 1373 Size: 32 42 32 2626 Fragments arranged by size: 2626 1890 1297 135 122 42 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2790 3228 3601 3648 3894 669 Size: 988 281 852 438 373 47 246 3079 Fragments arranged by size: 3079 988 852 438 373 281 246 47 HindIII A'AGCT_T Cuts at: 748 2165 748 Size: 1417 4887 HpaI GTT'AAC Cuts at: 2790 3228 3894 2790 Size: 438 666 5200 Fragments arranged by size: 5200 666 438 KpnI G_GTAC'C Cuts at: 2944 3629 2944 Size: 685 5619 MfeI C'AATT_G Cuts at: 3903 3903 Size: 6304 MluI A'CGCG_T Cuts at: 6180 6180 Size: 6304 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 3185 5418 5602 1305 Size: 1880 2233 184 2007 Fragments arranged by size: 2233 2007 1880 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 4482 Fragments arranged by size: 4482 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2150 4262 4621 4780 518 Size: 648 984 2112 359 159 2042 Fragments arranged by size: 2112 2042 984 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2222 2330 2362 2968 3324 3446 3720 4397 Size: 616 108 32 606 356 122 274 677 Cuts at: 4397 5545 5790 1606 Size: 1148 245 2120 Fragments arranged by size: 2120 1148 677 616 606 356 274 245 122 108 32 NcoI C'CATG_G Cuts at: 513 1065 2947 513 Size: 552 1882 3870 Fragments arranged by size: 3870 1882 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 6304 NheI G'CTAG_C Cuts at: 1052 2812 1052 Size: 1760 4544 NotI GC'GGCC_GC Cuts at: 3751 3751 Size: 6304 NspI r_CATG'y Cuts at: 1132 1229 3503 3662 5207 1132 Size: 97 2274 159 1545 2229 Fragments arranged by size: 2274 2229 1545 159 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 6304 PciI A'CATG_T Cuts at: 5203 5203 Size: 6304 PflMI CCAn_nnn'nTGG Cuts at: 1108 3546 1108 Size: 2438 3866 PfoI T'CCnGG_A Cuts at: 2470 2470 Size: 6304 PmeI GTTT'AAAC Cuts at: 3608 3608 Size: 6304 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3084 3116 4373 4405 5886 5918 3084 Size: 32 1257 32 1481 32 3470 Fragments arranged by size: 3470 1481 1257 32 32 32 PpuMI rG'GwC_Cy Cuts at: 2308 2308 Size: 6304 PshAI GACnn'nnGTC Cuts at: 1613 3119 1613 Size: 1506 4798 PsiI TTA'TAA Cuts at: 3874 3874 Size: 6304 PspOMI G'GGCC_C Cuts at: 2958 2958 Size: 6304 PstI C_TGCA'G Cuts at: 830 2322 2335 3244 3656 830 Size: 1492 13 909 412 3478 Fragments arranged by size: 3478 1492 909 412 13 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6304 PvuII CAG'CTG Cuts at: 2222 2330 2968 3446 2222 Size: 108 638 478 5080 Fragments arranged by size: 5080 638 478 108 SacI G_AGCT'C Cuts at: 2691 2691 Size: 6304 SalI G'TCGA_C Cuts at: 1936 3646 1936 Size: 1710 4594 SbfI CC_TGCA'GG Cuts at: 2322 3656 2322 Size: 1334 4970 ScaI AGT'ACT Cuts at: 4552 6264 4552 Size: 1712 4592 SfcI C'TryA_G Cuts at: 826 1045 2318 2331 2935 3240 3652 4787 5468 Size: 219 1273 13 604 305 412 1135 681 Cuts at: 5468 5659 826 Size: 191 1471 Fragments arranged by size: 1471 1273 1135 681 604 412 305 219 191 13 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6304 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6304 SmaI CCC'GGG Cuts at: 1872 3631 1872 Size: 1759 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 3574 4408 5309 5571 820 Size: 197 926 1631 834 901 262 1553 Fragments arranged by size: 1631 1553 926 901 834 262 197 SnaBI TAC'GTA Cuts at: 493 4095 493 Size: 3602 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 6304 SphI G_CATG'C Cuts at: 1132 3662 1132 Size: 2530 3774 SspI AAT'ATT Cuts at: 5 52 4228 5 Size: 47 4176 2081 Fragments arranged by size: 4176 2081 47 StuI AGG'CCT Cuts at: 1517 2842 1517 Size: 1325 4979 StyI C'CwwG_G Cuts at: 513 1065 2947 3743 513 Size: 552 1882 796 3074 Fragments arranged by size: 3074 1882 796 552 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2005 2654 4331 4348 4501 4686 5131 Size: 1255 425 649 1677 17 153 185 445 Cuts at: 5131 325 Size: 1498 Fragments arranged by size: 1677 1498 1255 649 445 425 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2142 4550 6262 Size: 274 80 33 51 1443 165 2408 1712 Cuts at: 6262 96 Size: 138 Fragments arranged by size: 2408 1712 1443 274 165 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2984 3016 6264 6296 2984 Size: 32 3248 32 2992 Fragments arranged by size: 3248 2992 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 2312 1316 Size: 996 5308 XbaI T'CTAG_A Cuts at: 3640 3640 Size: 6304 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2729 2729 Size: 6304 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 6304 XmaI C'CCGG_G Cuts at: 1870 3629 1870 Size: 1759 4545 XmnI GAAnn'nnTTC Cuts at: 4433 4433 Size: 6304 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 4112 276 Size: 53 83 186 936 2578 2468 Fragments arranged by size: 2578 2468 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BaeI BamHI BanI BanII BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PspOMI PstI PvuI PvuII SacI SalI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AfeI AloI AscI AvrII BbeI BfrBI BplI BsiWI BsmBI BstZ17I Bsu36I EcoRV FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PmlI PspXI PsrI RsrII SacII SanDI SapI SexAI SfiI SfoI SrfI SwaI