(Linear) (Six-base) MAPSORT of: KIEE6408.seq Check: 6441 from: 1 to: 1599 KIEE6408.orf With 182 enzymes: SgfI * March 16, 2015 11:31 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 0 561 1599 Size: 561 1038 AccI GT'mk_AC Cuts at: 0 912 1599 Size: 912 687 Acc65I G'GTAC_C Cuts at: 0 935 1599 Size: 935 664 AclI AA'CG_TT Cuts at: 0 781 1599 Size: 781 818 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 0 400 1599 Size: 400 1199 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 0 605 1599 Size: 605 994 AlwNI CAG_nnn'CTG Cuts at: 0 129 220 627 802 1599 Size: 129 91 407 175 797 Fragments arranged by size: 797 407 175 129 91 ApaI G_GGCC'C Cuts at: 0 957 1599 Size: 957 642 ApoI r'AATT_y Cuts at: 0 64 1599 Size: 64 1535 AvaI C'yCGr_G Cuts at: 0 1187 1599 Size: 1187 412 BaeI ACnnnnGTAyCnnnnnnn_nnnnn' Cuts at: 0 1427 1460 1599 Size: 1427 33 139 Fragments arranged by size: 1427 139 33 BanI G'GyrC_C Cuts at: 0 935 1387 1599 Size: 935 452 212 Fragments arranged by size: 935 452 212 BanII G_rGCy'C Cuts at: 0 686 957 1599 Size: 686 271 642 Fragments arranged by size: 686 642 271 BbsI GAAGACnn'nnnn_ Cuts at: 0 642 1389 1599 Size: 642 747 210 Fragments arranged by size: 747 642 210 BbvCI CC'TCA_GC Cuts at: 0 227 637 1599 Size: 227 410 962 Fragments arranged by size: 962 410 227 BlpI GC'TnA_GC Cuts at: 0 803 1599 Size: 803 796 Bme1580I G_kGCm'C Cuts at: 0 86 678 957 1599 Size: 86 592 279 642 Fragments arranged by size: 642 592 279 86 BmtI G_CTAG'C Cuts at: 0 811 1599 Size: 811 788 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 319 700 749 1599 Size: 319 381 49 850 Fragments arranged by size: 850 381 319 49 Bpu10I CC'TnA_GC Cuts at: 0 46 227 637 1599 Size: 46 181 410 962 Fragments arranged by size: 962 410 181 46 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 1590 1599 Size: 1590 9 BsaI GGTCTCn'nnnn_ Cuts at: 0 1536 1566 1599 Size: 1536 30 33 Fragments arranged by size: 1536 33 30 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 0 979 1009 1599 Size: 979 30 590 Fragments arranged by size: 979 590 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 0 613 1000 1599 Size: 613 387 599 Fragments arranged by size: 613 599 387 BseYI C'CCAG_C Cuts at: 0 179 1599 Size: 179 1420 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 0 76 142 373 652 1599 Size: 76 66 231 279 947 Fragments arranged by size: 947 279 231 76 66 BsiHKAI G_wGCw'C Cuts at: 0 686 1599 Size: 686 913 BsmI GAATG_Cn' Cuts at: 0 258 1599 Size: 258 1341 Bsp1286I G_dGCh'C Cuts at: 0 86 678 686 957 1599 Size: 86 592 8 271 642 Fragments arranged by size: 642 592 271 86 8 BspMI ACCTGCnnnn'nnnn_ Cuts at: 0 319 561 857 1599 Size: 319 242 296 742 Fragments arranged by size: 742 319 296 242 BsrBI CCG'CTC Cuts at: 0 521 1469 1599 Size: 521 948 130 Fragments arranged by size: 948 521 130 BsrDI GCAATG_nn' Cuts at: 0 19 491 968 1599 Size: 19 472 477 631 Fragments arranged by size: 631 477 472 19 BssSI C'ACGA_G Cuts at: 0 1085 1599 Size: 1085 514 BstXI CCAn_nnnn'nTGG Cuts at: 0 192 1599 Size: 192 1407 BtgI C'CryG_G Cuts at: 0 942 1599 Size: 942 657 DraIII CAC_nnn'GTG Cuts at: 0 249 1599 Size: 249 1350 DrdI GACnn_nn'nnGTC Cuts at: 0 210 1599 Size: 210 1389 EarI CTCTTCn'nnn_ Cuts at: 0 761 913 1599 Size: 761 152 686 Fragments arranged by size: 761 686 152 EcoICRI GAG'CTC Cuts at: 0 684 1599 Size: 684 915 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 0 319 400 700 749 1599 Size: 319 81 300 49 850 Fragments arranged by size: 850 319 300 81 49 EcoNI CCTnn'n_nnAGG Cuts at: 0 148 1599 Size: 148 1451 EcoO109I rG'GnC_Cy Cuts at: 0 303 953 954 1599 Size: 303 650 1 645 Fragments arranged by size: 650 645 303 1 EcoRI G'AATT_C Cuts at: 0 64 1599 Size: 64 1535 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 0 178 1599 Size: 178 1421 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 0 864 896 1018 1050 1599 Size: 864 32 122 32 549 Fragments arranged by size: 864 549 122 32 32 HincII GTy'rAC Cuts at: 0 785 1223 1596 1599 Size: 785 438 373 3 Fragments arranged by size: 785 438 373 3 HindIII A'AGCT_T Cuts at: 0 160 1599 Size: 160 1439 HpaI GTT'AAC Cuts at: 0 785 1223 1599 Size: 785 438 376 Fragments arranged by size: 785 438 376 KpnI G_GTAC'C Cuts at: 0 939 1599 Size: 939 660 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 0 1180 1599 Size: 1180 419 MslI CAynn'nnrTG Cuts at: 0 145 1599 Size: 145 1454 MspA1I CmG'CkG Cuts at: 0 217 325 357 963 1319 1441 1599 Size: 217 108 32 606 356 122 158 Fragments arranged by size: 606 356 217 158 122 108 32 NcoI C'CATG_G Cuts at: 0 942 1599 Size: 942 657 NheI G'CTAG_C Cuts at: 0 807 1599 Size: 807 792 NspI r_CATG'y Cuts at: 0 1498 1599 Size: 1498 101 PflMI CCAn_nnn'nTGG Cuts at: 0 1541 1599 Size: 1541 58 PfoI T'CCnGG_A Cuts at: 0 465 1599 Size: 465 1134 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 0 1079 1111 1599 Size: 1079 32 488 Fragments arranged by size: 1079 488 32 PpuMI rG'GwC_Cy Cuts at: 0 303 1599 Size: 303 1296 PshAI GACnn'nnGTC Cuts at: 0 1114 1599 Size: 1114 485 PspOMI G'GGCC_C Cuts at: 0 953 1599 Size: 953 646 PstI C_TGCA'G Cuts at: 0 317 330 1239 1599 Size: 317 13 909 360 Fragments arranged by size: 909 360 317 13 PvuII CAG'CTG Cuts at: 0 217 325 963 1441 1599 Size: 217 108 638 478 158 Fragments arranged by size: 638 478 217 158 108 SacI G_AGCT'C Cuts at: 0 686 1599 Size: 686 913 SbfI CC_TGCA'GG Cuts at: 0 317 1599 Size: 317 1282 SfcI C'TryA_G Cuts at: 0 313 326 930 1235 1599 Size: 313 13 604 305 364 Fragments arranged by size: 604 364 313 305 13 SmlI C'TyrA_G Cuts at: 0 1569 1599 Size: 1569 30 StuI AGG'CCT Cuts at: 0 837 1599 Size: 837 762 StyI C'CwwG_G Cuts at: 0 942 1599 Size: 942 657 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 0 649 1599 Size: 649 950 TatI w'GTAC_w Cuts at: 0 137 1599 Size: 137 1462 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 0 979 1011 1599 Size: 979 32 588 Fragments arranged by size: 979 588 32 Tth111I GACn'n_nGTC Cuts at: 0 307 1599 Size: 307 1292 XcmI CCAnnnn_n'nnnnTGG Cuts at: 0 724 1599 Size: 724 875 Enzymes that do cut: AarI AccI Acc65I AclI AcuI AlfI AlwNI ApaI ApoI AvaI BaeI BanI BanII BbsI BbvCI BlpI Bme1580I BmtI BpmI Bpu10I BpuEI BsaI BsaXI BseRI BseYI BsgI BsiHKAI BsmI Bsp1286I BspMI BsrBI BsrDI BssSI BstXI BtgI DraIII DrdI EarI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI Hin4I HincII HindIII HpaI KpnI MmeI MslI MspA1I NcoI NheI NspI PflMI PfoI PpiI PpuMI PshAI PspOMI PstI PvuII SacI SbfI SfcI SmlI StuI StyI TaqII TatI TstI Tth111I XcmI Enzymes that do not cut: AatII AfeI AflII AflIII AgeI AhdI AleI AloI ApaLI AscI AseI AsiSI AvrII BamHI BbeI BcgI BciVI BclI BfrBI BglI BglII BmgBI BmrI BplI BsaAI BsaBI BsaHI BsaWI BsiEI BsiWI BsmBI BspEI BspHI BsrFI BsrGI BssHII BstAPI BstBI BstEII BstYI BstZ17I Bsu36I BtgZI BtsI ClaI CspCI DraI EaeI EagI EciI EcoRV FseI FspI FspAI HaeII KasI MfeI MluI MscI NaeI NarI NdeI NgoMIV NotI NruI NsiI PacI PasI PciI PmeI PmlI PsiI PspXI PsrI PvuI RsrII SacII SalI SanDI SapI ScaI SexAI SfiI SfoI SgfI SgrAI SmaI SnaBI SpeI SphI SrfI SspI SwaI XbaI XhoI XmaI XmnI ZraI