################################## # NO CUT ENZYMES ( 89 enzymes) # ################################## AatII Acc65I AccI AccIII AclI AflII AflIII AgeI AhdI Alw44I Aor51HI ApaLI AscI AseI AsnI Asp700I Asp718I AspEI AviII BbeI BbrPI BcgI BciVI BfrI BglII BsaAI BsaBI BseAI BsmBI BspEI BspLU11I BssHII BssSI Bst1107I BstBI BstZ17I CpoI DraI DrdI Eam1105I Eco47III EcoRV EcoT22I FseI FspI HindIII HpaI KasI KpnI KspI MamI MluI MroI NaeI NarI NdeI NgoMIV NheI NotI NruI NsiI NspV PacI PinAI PmaCI PmeI PmlI PshBI Psp1406I PvuI RsrII SacI SacII SalI SbfI SfiI SfoI SfuI SgrAI SmaI SnaBI SpeI SphI Sse8387I SwaI XbaI XmaCI XmaI XmnI ################################### # ONE CUT ENZYMES ( 45 enzymes) # ################################### AccII 3270 CGCG CG^CG AocI 713 CCTCAGG CC^T[ACGT]AGG ApaI 2671 GGGCCC GGGCC^C AspI 2389 GACAGTGTC GAC[ACGT]^[ACGT][ACGT]GTC AvrII 2368 CCTAGG C^CTAGG BlnI 2368 CCTAGG C^CTAGG BlpI 482 GCTCAGC GC^T[ACGT]AGC Bpu1102I 482 GCTCAGC GC^T[ACGT]AGC BsiEI 3276 CGGCCG CG[GA][CT]^CG BsiWI 402 CGTACG C^GTACG BspDI 2759 ATCGAT AT^CGAT BspHI 3317 TCATGA T^CATGA BsrBI 214 GAGCGG CCGCTC(-3/-3) BsrFI 3278 GCCGGT [GA]^CCGG[CT] BstAPI 2246 GCAGGCGGTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC BstEII 2001 GGTCACC G^GT[ACGT]ACC BstPI 2001 GGTCACC G^GT[ACGT]ACC BstUI 3270 CGCG CG^CG Bsu36I 713 CCTCAGG CC^T[ACGT]AGG CelII 482 GCTCAGC GC^T[ACGT]AGC Cfr10I 3278 GCCGGT [GA]^CCGG[CT] ClaI 2759 ATCGAT AT^CGAT DraIII 2717 CACCCAGTG CAC[ACGT][ACGT][ACGT]^GTG EagI 3276 CGGCCG C^GGCCG EclXI 3276 CGGCCG C^GGCCG Eco52I 3276 CGGCCG C^GGCCG Eco81I 713 CCTCAGG CC^T[ACGT]AGG EcoO65I 2001 GGTCACC G^GT[ACGT]ACC EcoRI 2848 GAATTC G^AATTC HaeII 3488 AGCGCC [GA]GCGC^[CT] MfeI 2537 CAATTG C^AATTG MunI 2537 CAATTG C^AATTG MvnI 3270 CGCG CG^CG PaeR7I 3453 CTCGAG C^TCGAG PflFI 2389 GACAGTGTC GAC[ACGT]^[ACGT][ACGT]GTC PpuMI 3245 AGGACCC [GA]G^G[AT]CC[CT] RcaI 3317 TCATGA T^CATGA SapI 3321 GAAGAGC GCTCTTC(1/4) ScaI 1118 AGTACT AGT^ACT SexAI 3134 ACCTGGT A^CC[AT]GGT SmlI 3453 CTCGAG C^T[CT][GA]AG SspI 1891 AATATT AAT^ATT StuI 14 AGGCCT AGG^CCT Tth111I 2389 GACAGTGTC GAC[ACGT]^[ACGT][ACGT]GTC XhoI 3453 CTCGAG C^TCGAG ################################### # TWO CUT ENZYMES ( 15 enzymes) # ################################### AvaI 2978 CTCGGG C^[CT]CG[GA]G 3453 CTCGAG BclI 3045 TGATCA T^GATCA 3545 TGATCA BsaI 1747 GAGACC GGTCTC(1/5) 464 GGTCTC BsaWI 2276 ACCGGA [AT]^CCGG[AT] 2735 TCCGGT BsmI 636 GAATGC GAATGC(1/-1) 1257 GAATGC BsoBI 2978 CTCGGG C^[CT]CG[GA]G 3453 CTCGAG Bsp1407I 598 TGTACA T^GTACA 1529 TGTACA BspMI 300 ACCTGC ACCTGC(4/8) 616 ACCTGC BsrGI 598 TGTACA T^GTACA 1529 TGTACA FbaI 3045 TGATCA T^GATCA 3545 TGATCA MaeII 1283 ACGT A^CGT 1966 ACGT PflMI 11 CCAAGGCCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 287 CCACTGACTGG PshAI 501 GACTGGGGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC 2052 GACCCAGGTC SspBI 598 TGTACA T^GTACA 1529 TGTACA Van91I 11 CCAAGGCCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 287 CCACTGACTGG ######################################### # 3 OR MORE CUT ENZYMES ( 109 enzymes) # ######################################### AciI AcsI AcyI AfaI AluI AlwI AlwNI ApoI AspHI AvaII BalI BamHI BanI BanII BbsI BbvCI BbvI BcnI BfaI BglI BmyI BpmI BpuAI BsaHI BsaJI BseRI BsgI BsiHKAI BsiYI BslI BsmAI BsmFI Bsp1286I BsrDI BsrI BssKI BstF5I BstNI BstXI BstYI Cac8I CfoI Cfr13I DdeI DpnII DraII DsaI EaeI EarI EcoNI EcoO109I EcoRII EcoT14I FauI Fnu4HI FokI HaeIII HapII HgaI HhaI Hin1I HinP1I HincII HindII HinfI HpaII HphI ItaI Ksp632I MaeI MaeIII MboI MboII MflI MluNI MnlI MscI MseI MslI MspA1I MspI MvaI MwoI NciI NcoI NdeII NlaIII NlaIV NspI PleI PstI PvuII RsaI Sau3AI Sau96I ScrFI SfaNI SfcI StyI TaqI TfiI Tru9I TseI Tsp45I Tsp509I TspRI TthHB8I XcmI XhoII