Restriction map for
pFN21AE1391
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# NO CUT ENZYMES ( 111 enzymes) #
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AatII Acc65I AclI AcyI AflII
AgeI Aor51HI ApaI AscI AseI
AsnI Asp700I Asp718I AspI AviII
AvrII BbeI BbrPI BcgI BfrI
BlnI BlpI Bpu1102I BsaAI BsaBI
BsaHI BsaI BsiWI BsmBI BspDI
BspHI BspLU11I BsrBI BsrFI BssHII
BssSI Bst1107I BstAPI BstBI BstEII
BstPI BstXI BstZ17I CelII CfoI
Cfr10I ClaI CpoI DraIII DrdI
Eco47III EcoNI EcoO65I EcoRV FseI
FspI HaeII HhaI Hin1I HinP1I
HincII HindII HpaI KasI KpnI
KspI MaeII MamI MfeI MunI
NaeI NarI NdeI NgoMIV NheI
NotI NruI NspV PacI PaeR7I
PflFI PinAI PmaCI PmeI PmlI
PshBI Psp1406I PvuI RcaI RsrII
SacII SalI SapI SbfI SexAI
SfiI SfoI SfuI SgrAI SmaI
SnaBI SpeI SphI Sse8387I StuI
SwaI Tth111I XhoI XmaCI XmaI
XmnI
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# ONE CUT ENZYMES ( 54 enzymes) #
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AccI 1690 GTAGAC GT^[AC][GT]AC
AccII 1219 CGCG CG^CG
AccIII 1964 TCCGGA T^CCGGA
AflIII 1218 ACGCGT A^C[GA][CT]GT
Alw44I 1257 GTGCAC G^TGCAC
AocI 1518 CCTCAGG CC^T[ACGT]AGG
ApaLI 1257 GTGCAC G^TGCAC
AvaI 1757 CCCGAG C^[CT]CG[GA]G
BalI 2 TGGCCA TGG^CCA
BamHI 944 GGATCC G^GATCC
BanI 1636 GGCACC G^G[CT][GA]CC
BbvCI 1933 CCTCAGC CCTCAGC(-5/-2)
BciVI 2038 GGATAC GTATCC(6/5)
BcnI 1925 CCGGG CC^[GC]GG
BsaWI 1964 TCCGGA [AT]^CCGG[AT]
BseAI 1964 TCCGGA T^CCGGA
BsiEI 2517 CGGCCG CG[GA][CT]^CG
BsoBI 1757 CCCGAG C^[CT]CG[GA]G
BspEI 1964 TCCGGA T^CCGGA
BspMI 2596 ACCTGC ACCTGC(4/8)
BstUI 1219 CGCG CG^CG
Bsu36I 1518 CCTCAGG CC^T[ACGT]AGG
DraI 2469 TTTAAA TTT^AAA
DraII 2068 GGGACCT [GA]G^G[ACGT]CC[CT]
DsaI 265 CCATGG C^C[GA][CT]GG
EagI 2517 CGGCCG C^GGCCG
EarI 406 GAAGAG CTCTTC(1/4)
EclXI 2517 CGGCCG C^GGCCG
Eco52I 2517 CGGCCG C^GGCCG
Eco81I 1518 CCTCAGG CC^T[ACGT]AGG
EcoO109I 2068 GGGACCT [GA]G^G[ACGT]CC[CT]
EcoRI 2244 GAATTC G^AATTC
EcoT14I 265 CCATGG C^C[AT][AT]GG
FauI 2440 CCCGC CCCGC(4/6)
HindIII 1526 AAGCTT A^AGCTT
Ksp632I 406 GAAGAG CTCTTC(1/4)
MluI 1218 ACGCGT A^CGCGT
MluNI 2 TGGCCA TGG^CCA
MroI 1964 TCCGGA T^CCGGA
MscI 2 TGGCCA TGG^CCA
MslI 2352 CATTTGTATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
MvnI 1219 CGCG CG^CG
NciI 1925 CCGGG CC^[GC]GG
NcoI 265 CCATGG C^CATGG
NspI 2233 ACATGC [GA]CATG^[CT]
PflMI 1948 CCAGAAAATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PpuMI 2068 GGGACCT [GA]G^G[AT]CC[CT]
PshAI 263 GACCATGGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
PstI 734 CTGCAG CTGCA^G
SmlI 813 CTCAAG C^T[CT][GA]AG
SspI 1068 AATATT AAT^ATT
StyI 265 CCATGG C^C[AT][AT]GG
Van91I 1948 CCAGAAAATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XbaI 1038 TCTAGA T^CTAGA
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# TWO CUT ENZYMES ( 15 enzymes) #
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AhdI 8 GACGCTCTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
1608 GACTGAATGTC
AlwNI 1604 CAGGGACTG CAG[ACGT][ACGT][ACGT]^CTG
2339 CAGGATCTG
AspEI 8 GACGCTCTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
1608 GACTGAATGTC
BclI 44 TGATCA T^GATCA
72 TGATCA
Bsp1407I 567 TGTACA T^GTACA
1058 TGTACA
BsrGI 567 TGTACA T^GTACA
1058 TGTACA
Eam1105I 8 GACGCTCTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
1608 GACTGAATGTC
FbaI 44 TGATCA T^GATCA
72 TGATCA
HgaI 8 GACGC GACGC(5/10)
321 GCGTC
PvuII 99 CAGCTG CAG^CTG
2320 CAGCTG
SacI 828 GAGCTC GAGCT^C
1380 GAGCTC
ScaI 161 AGTACT AGT^ACT
2045 AGTACT
SfcI 734 CTGCAG C^T[GA][CT]AG
2376 CTGTAG
SspBI 567 TGTACA T^GTACA
1058 TGTACA
XcmI 1466 CCATCGTGGACTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
2062 CCAGACGGGACCTGG
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# 3 OR MORE CUT ENZYMES ( 78 enzymes) #
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AciI AcsI AfaI AluI AlwI
ApoI AspHI AvaII BanII BbsI
BbvI BfaI BglI BglII BmyI
BpmI BpuAI BsaJI BseRI BsgI
BsiHKAI BsiYI BslI BsmAI BsmFI
BsmI Bsp1286I BsrDI BsrI BssKI
BstF5I BstNI BstYI Cac8I Cfr13I
DdeI DpnII EaeI EcoRII EcoT22I
Fnu4HI FokI HaeIII HapII HinfI
HpaII HphI ItaI MaeI MaeIII
MboI MboII MflI MnlI MseI
MspA1I MspI MvaI MwoI NdeII
NlaIII NlaIV NsiI PleI RsaI
Sau3AI Sau96I ScrFI SfaNI TaqI
TfiI Tru9I TseI Tsp45I Tsp509I
TspRI TthHB8I XhoII