(Circular) (Six-base) MAPSORT of: pFN21AE1391.seq Check: 2093 from: 1 to: 7507 pFN21AE1391 7507 bp With 182 enzymes: SgfI * August 16, 2010 14:43 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 4610 4610 Size: 7507 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 5317 278 Size: 53 83 186 936 3781 2468 Fragments arranged by size: 3781 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3696 4850 1937 Size: 1759 1154 4594 Fragments arranged by size: 4594 1759 1154 Acc65I G'GTAC_C Cuts at: 4828 4828 Size: 7507 AclI AA'CG_TT Cuts at: 1563 5634 6007 1563 Size: 4071 373 3063 Fragments arranged by size: 4071 3063 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 4039 5570 6954 7222 1632 Size: 2407 1531 1384 268 1917 Fragments arranged by size: 2407 1917 1531 1384 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 7310 AflIII A'CryG_T Cuts at: 1203 3223 6406 7383 1203 Size: 2020 3183 977 1327 Fragments arranged by size: 3183 2020 1327 977 AgeI A'CCGG_T Cuts at: 6338 6338 Size: 7507 AhdI GACnn_n'nnGTC Cuts at: 2018 3618 6236 2018 Size: 1600 2618 3289 Fragments arranged by size: 3289 2618 1600 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 7507 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 3561 1184 Size: 2377 5130 AlwNI CAG_nnn'CTG Cuts at: 3614 4349 5250 6822 3614 Size: 735 901 1572 4299 Fragments arranged by size: 4299 1572 901 735 ApaLI G'TGCA_C Cuts at: 3262 5564 6720 3262 Size: 2302 1156 4049 Fragments arranged by size: 4049 2302 1156 ApoI r'AATT_y Cuts at: 1493 2432 4011 4249 4816 5042 5194 7303 1493 Size: 939 1579 238 567 226 152 2109 1697 Fragments arranged by size: 2109 1697 1579 939 567 238 226 152 AseI AT'TA_AT Cuts at: 160 1032 6061 160 Size: 872 5029 1606 Fragments arranged by size: 5029 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 7507 AvaI C'yCGr_G Cuts at: 1870 1943 3762 4832 1870 Size: 73 1819 1070 4545 Fragments arranged by size: 4545 1819 1070 73 BamHI G'GATC_C Cuts at: 2949 4837 5204 2949 Size: 1888 367 5252 Fragments arranged by size: 5252 1888 367 BanI G'GyrC_C Cuts at: 618 943 1160 1787 3641 4828 6283 618 Size: 325 217 627 1854 1187 1455 1842 Fragments arranged by size: 1854 1842 1455 1187 627 325 217 BanII G_rGCy'C Cuts at: 1391 2616 2837 3389 4826 1391 Size: 1225 221 552 1437 4072 Fragments arranged by size: 4072 1437 1225 552 221 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 3410 3711 3837 4383 928 Size: 971 1511 301 126 546 4052 Fragments arranged by size: 4052 1511 971 546 301 126 BbvCI CC'TCA_GC Cuts at: 3939 3939 Size: 7507 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 5698 5732 1903 Size: 34 3761 34 3678 Fragments arranged by size: 3761 3678 34 34 BciVI GTATCCnnnnn_n' Cuts at: 4037 5399 6609 4037 Size: 1362 1210 4935 Fragments arranged by size: 4935 1362 1210 BclI T'GATC_A Cuts at: 1548 2049 2077 1548 Size: 501 28 6978 Fragments arranged by size: 6978 501 28 BfrBI ATG'CAT Cuts at: 2087 2385 4356 2087 Size: 298 1971 5238 Fragments arranged by size: 5238 1971 298 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 4026 4206 4521 Size: 107 122 71 631 605 2354 180 315 Cuts at: 4521 6118 136 Size: 1597 1525 Fragments arranged by size: 2354 1597 1525 631 605 315 180 122 107 71 BglII A'GATC_T Cuts at: 2315 2995 4399 7502 2315 Size: 680 1404 3103 2320 Fragments arranged by size: 3103 2320 1404 680 BlpI GC'TnA_GC Cuts at: 4924 4924 Size: 7507 Bme1580I G_kGCm'C Cuts at: 1790 3266 5568 6724 1790 Size: 1476 2302 1156 2573 Fragments arranged by size: 2573 2302 1476 1156 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 7507 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2325 4621 5238 Size: 416 53 83 399 24 908 2296 617 Cuts at: 5238 6196 442 Size: 958 1753 Fragments arranged by size: 2296 1753 958 908 617 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 7507 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2667 3416 3657 3745 3936 4099 4353 6167 Size: 1428 749 241 88 191 163 254 1814 Cuts at: 6167 1239 Size: 2579 Fragments arranged by size: 2579 1814 1428 749 254 241 191 163 88 Bpu10I CC'TnA_GC Cuts at: 3939 3939 Size: 7507 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2803 5632 6497 6795 2803 Size: 2829 865 298 3515 Fragments arranged by size: 3515 2829 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 6170 882 Size: 617 4671 2219 Fragments arranged by size: 4671 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 5298 493 Size: 711 4094 2702 Fragments arranged by size: 4094 2702 711 BsaBI GATnn'nnATC Cuts at: 7501 7501 Size: 7507 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 5314 5696 6331 275 Size: 53 83 186 936 3781 382 635 1451 Fragments arranged by size: 3781 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 3969 5940 6338 6612 6759 7170 3969 Size: 1971 398 274 147 411 4306 Fragments arranged by size: 4306 1971 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 3376 3406 3718 3748 3990 Size: 30 165 30 1973 30 312 30 242 Cuts at: 3990 4020 4406 4436 1178 Size: 30 386 30 4249 Fragments arranged by size: 4249 1973 386 312 242 165 30 30 30 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2634 3413 3807 1186 Size: 1448 779 394 4886 Fragments arranged by size: 4886 1448 779 394 BseYI C'CCAG_C Cuts at: 4220 4327 6710 7206 4220 Size: 107 2383 496 4521 Fragments arranged by size: 4521 2383 496 107 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2984 3522 3820 4151 1163 Size: 573 1248 538 298 331 4519 Fragments arranged by size: 4519 1248 573 538 331 298 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 4525 4957 5718 6746 1540 Size: 275 186 2524 432 761 1028 2301 Fragments arranged by size: 2524 2301 1028 761 432 275 186 BsiHKAI G_wGCw'C Cuts at: 2837 3266 3389 3577 5568 5653 6724 2837 Size: 429 123 188 1991 85 1071 3620 Fragments arranged by size: 3620 1991 1071 429 188 123 85 BsmI GAATG_Cn' Cuts at: 2047 2385 2480 3773 4103 4358 5018 5111 2047 Size: 338 95 1293 330 255 660 93 4443 Fragments arranged by size: 4443 1293 660 338 330 255 95 93 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2616 2837 3266 3389 3577 4826 5568 Size: 399 826 221 429 123 188 1249 742 Cuts at: 5568 5653 6724 1391 Size: 85 1071 2174 Fragments arranged by size: 2174 1249 1071 826 742 429 399 221 188 123 85 BspEI T'CCGG_A Cuts at: 3969 7170 3969 Size: 3201 4306 BspHI T'CATG_A Cuts at: 5396 7126 5396 Size: 1730 5777 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 4610 4862 844 Size: 3766 252 3489 Fragments arranged by size: 3766 3489 252 BsrBI CCG'CTC Cuts at: 1394 1823 5394 1394 Size: 429 3571 3507 Fragments arranged by size: 3571 3507 429 BsrDI GCAATG_nn' Cuts at: 65 1248 2457 2485 2593 6002 6176 65 Size: 1183 1209 28 108 3409 174 1396 Fragments arranged by size: 3409 1396 1209 1183 174 108 28 BsrFI r'CCGG_y Cuts at: 1700 1810 6151 6338 1700 Size: 110 4341 187 2869 Fragments arranged by size: 4341 2869 187 110 BsrGI T'GTAC_A Cuts at: 96 2572 3063 96 Size: 2476 491 4540 Fragments arranged by size: 4540 2476 491 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 7507 BssSI C'ACGA_G Cuts at: 5567 6579 5567 Size: 1012 6495 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 7507 BstBI TT'CG_AA Cuts at: 6311 6311 Size: 7507 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 7507 BstYI r'GATC_y Cuts at: 1972 2315 2949 2995 4346 4399 4837 5204 5589 Size: 343 634 46 1351 53 438 367 385 Cuts at: 5589 5606 7058 7144 7156 7502 1972 Size: 17 1452 86 12 346 1977 Fragments arranged by size: 1977 1452 1351 634 438 385 367 346 343 86 53 46 17 12 Bsu36I CC'TnA_GG Cuts at: 3524 3524 Size: 7507 BtgI C'CryG_G Cuts at: 513 1065 2004 2270 513 Size: 552 939 266 5750 Fragments arranged by size: 5750 939 552 266 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 6768 Fragments arranged by size: 6768 683 56 BtsI GCAGTG_nn' Cuts at: 3157 3510 5023 5817 5837 3157 Size: 353 1513 794 20 4827 Fragments arranged by size: 4827 1513 794 353 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 7507 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 7472 DraI TTT'AAA Cuts at: 4476 4811 4476 Size: 335 7172 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 6514 809 Size: 507 5198 1802 Fragments arranged by size: 5198 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2007 2449 2703 Size: 54 1425 50 275 18 177 442 254 Cuts at: 2703 3592 4522 4803 4954 5843 7472 8 Size: 889 930 281 151 889 1629 43 Fragments arranged by size: 1629 1425 930 889 889 442 281 275 254 177 151 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 4522 4954 1537 Size: 275 2710 432 4090 Fragments arranged by size: 4090 2710 432 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2406 5437 1353 Size: 1053 3031 3423 Fragments arranged by size: 3423 3031 1053 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 6094 6468 6614 387 Size: 1064 138 4505 374 146 1280 Fragments arranged by size: 4505 1280 1064 374 146 138 EcoICRI GAG'CTC Cuts at: 2835 3387 2835 Size: 552 6955 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2667 3416 3657 3745 3936 4039 4099 Size: 393 1035 749 241 88 191 103 60 Cuts at: 4099 4353 5570 6167 6954 7222 1239 Size: 254 1217 597 787 268 1524 Fragments arranged by size: 1524 1217 1035 787 749 597 393 268 254 241 191 103 88 60 EcoO109I rG'GnC_Cy Cuts at: 4074 4074 Size: 7507 EcoRI G'AATT_C Cuts at: 4249 4816 5194 4249 Size: 567 378 6562 Fragments arranged by size: 6562 567 378 FspI TGC'GCA Cuts at: 6013 6013 Size: 7507 HaeII r_GCGC'y Cuts at: 1678 6654 1678 Size: 4976 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2004 2036 2226 2258 2610 Size: 32 135 32 432 32 190 32 352 Cuts at: 2610 2642 2671 2703 3376 3408 3588 3604 3620 Size: 32 29 32 673 32 180 16 16 Cuts at: 3620 3636 3653 3668 3685 3700 4406 4438 6148 Size: 16 17 15 17 15 706 32 1710 Cuts at: 6148 6180 6222 6254 1373 Size: 32 42 32 2626 Fragments arranged by size: 2626 1710 706 673 432 352 190 180 135 42 32 32 32 32 32 32 32 32 32 32 29 17 17 16 16 16 15 15 HincII GTy'rAC Cuts at: 669 1657 1938 4851 5097 669 Size: 988 281 2913 246 3079 Fragments arranged by size: 3079 2913 988 281 246 HindIII A'AGCT_T Cuts at: 748 3531 748 Size: 2783 4724 HpaI GTT'AAC Cuts at: 5097 5097 Size: 7507 KpnI G_GTAC'C Cuts at: 4832 4832 Size: 7507 MfeI C'AATT_G Cuts at: 5106 5106 Size: 7507 MluI A'CGCG_T Cuts at: 3223 7383 3223 Size: 4160 3347 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 3976 4021 4449 4590 6621 6805 1305 Size: 2671 45 428 141 2031 184 2007 Fragments arranged by size: 2671 2031 2007 428 184 141 45 MscI TGG'CCA Cuts at: 10 64 1489 1832 2009 10 Size: 54 1425 343 177 5508 Fragments arranged by size: 5508 1425 343 177 54 MslI CAynn'nnrTG Cuts at: 518 1166 4361 5465 5824 5983 518 Size: 648 3195 1104 359 159 2042 Fragments arranged by size: 3195 2042 1104 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2106 2454 4327 4923 5600 6748 6993 1606 Size: 500 348 1873 596 677 1148 245 2120 Fragments arranged by size: 2120 1873 1148 677 596 500 348 245 NcoI C'CATG_G Cuts at: 513 1065 2004 2270 513 Size: 552 939 266 5750 Fragments arranged by size: 5750 939 552 266 NdeI CA'TA_TG Cuts at: 387 387 Size: 7507 NheI G'CTAG_C Cuts at: 1052 1052 Size: 7507 NotI GC'GGCC_GC Cuts at: 4954 4954 Size: 7507 NsiI A_TGCA'T Cuts at: 2089 2387 4358 2089 Size: 298 1971 5238 Fragments arranged by size: 5238 1971 298 NspI r_CATG'y Cuts at: 1132 1229 4242 4865 6410 1132 Size: 97 3013 623 1545 2229 Fragments arranged by size: 3013 2229 1545 623 97 PasI CC'CwG_GG Cuts at: 1348 3115 1348 Size: 1767 5740 PciI A'CATG_T Cuts at: 6406 6406 Size: 7507 PflMI CCAn_nnn'nTGG Cuts at: 1108 3959 1108 Size: 2851 4656 PfoI T'CCnGG_A Cuts at: 3722 4253 4789 3722 Size: 531 536 6440 Fragments arranged by size: 6440 536 531 PmeI GTTT'AAAC Cuts at: 4811 4811 Size: 7507 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 5576 5608 7089 7121 5576 Size: 32 1481 32 5962 Fragments arranged by size: 5962 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 4074 4074 Size: 7507 PshAI GACnn'nnGTC Cuts at: 1613 2272 1613 Size: 659 6848 PsiI TTA'TAA Cuts at: 3681 3747 5077 3681 Size: 66 1330 6111 Fragments arranged by size: 6111 1330 66 PstI C_TGCA'G Cuts at: 830 2743 4859 830 Size: 1913 2116 3478 Fragments arranged by size: 3478 2116 1913 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 7507 PvuII CAG'CTG Cuts at: 2106 4327 2106 Size: 2221 5286 SacI G_AGCT'C Cuts at: 2837 3389 2837 Size: 552 6955 SalI G'TCGA_C Cuts at: 1936 4849 1936 Size: 2913 4594 SbfI CC_TGCA'GG Cuts at: 4859 4859 Size: 7507 ScaI AGT'ACT Cuts at: 2168 4052 5755 7467 2168 Size: 1884 1703 1712 2208 Fragments arranged by size: 2208 1884 1712 1703 SfcI C'TryA_G Cuts at: 826 1045 2739 4381 4855 5990 6671 6862 826 Size: 219 1694 1642 474 1135 681 191 1471 Fragments arranged by size: 1694 1642 1471 1135 681 474 219 191 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 7507 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 7507 SmaI CCC'GGG Cuts at: 1872 4834 1872 Size: 2962 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2818 5611 6512 6774 820 Size: 197 926 875 2793 901 262 1553 Fragments arranged by size: 2793 1553 926 901 875 262 197 SnaBI TAC'GTA Cuts at: 493 5298 493 Size: 4805 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 7507 SphI G_CATG'C Cuts at: 1132 4865 1132 Size: 3733 3774 SspI AAT'ATT Cuts at: 5 52 3075 5431 5 Size: 47 3023 2356 2081 Fragments arranged by size: 3023 2356 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 7507 StyI C'CwwG_G Cuts at: 513 1065 2004 2270 4946 513 Size: 552 939 266 2676 3074 Fragments arranged by size: 3074 2676 939 552 266 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 4279 5534 5551 5704 5889 6334 325 Size: 1255 2699 1255 17 153 185 445 1498 Fragments arranged by size: 2699 1498 1255 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2025 2166 2572 Size: 274 80 33 51 1443 48 141 406 Cuts at: 2572 3063 4050 5753 7465 96 Size: 491 987 1703 1712 138 Fragments arranged by size: 1712 1703 1443 987 491 406 274 141 138 80 51 48 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 3245 3277 3718 3750 3790 3822 7467 7499 3245 Size: 32 441 32 40 32 3645 32 3253 Fragments arranged by size: 3645 3253 441 40 32 32 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 7507 XbaI T'CTAG_A Cuts at: 3043 4843 3043 Size: 1800 5707 XcmI CCAnnnn_n'nnnnTGG Cuts at: 3478 4074 3478 Size: 596 6911 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 7507 XmaI C'CCGG_G Cuts at: 1870 4832 1870 Size: 2962 4545 XmnI GAAnn'nnTTC Cuts at: 5636 5636 Size: 7507 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 5315 276 Size: 53 83 186 936 3781 2468 Fragments arranged by size: 3781 2468 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BbvCI BcgI BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NsiI NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PstI PvuI PvuII SacI SalI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AfeI AloI ApaI AscI AvrII BaeI BbeI BplI BsiWI BsmBI BstXI BstZ17I DraIII EcoNI EcoRV FalI FseI FspAI KasI NaeI NarI NgoMIV NruI PacI PmlI PspOMI PspXI PsrI RsrII SacII SanDI SapI SexAI SfiI SfoI SrfI SwaI