################################## # NO CUT ENZYMES ( 166 enzymes) # ################################## AatII Acc65I AccII AccIII AclI AcyI AflII AgeI AhdI Alw44I AlwNI Aor51HI ApaI ApaLI AscI AseI AsnI Asp700I Asp718I AspEI AspHI AspI AvaI AviII AvrII BalI BamHI BanI BanII BbeI BbrPI BbvCI BcgI BciVI BclI BcnI BfrI BglI BglII BlnI BlpI BmyI BpmI Bpu1102I BsaAI BsaBI BsaHI BsaI BseAI BseRI BsiEI BsiHKAI BsiWI BsmAI BsmBI BsmFI BsoBI Bsp1286I BspDI BspEI BspHI BsrBI BsrFI BssHII BssSI Bst1107I BstAPI BstUI BstXI BstZ17I CelII CfoI Cfr10I ClaI CpoI DraIII DrdI EaeI EagI Eam1105I EclXI Eco47III Eco52I EcoNI EcoRI EcoRV EcoT14I FauI FbaI FseI FspI HaeII HaeIII HgaI HhaI Hin1I HinP1I HincII HindII HindIII HpaI KasI KpnI KspI MamI MfeI MluI MluNI MroI MscI MspA1I MunI MvnI MwoI NaeI NarI NciI NcoI NdeI NgoMIV NheI NlaIV NotI NruI PacI PaeR7I PflFI PflMI PinAI PleI PmaCI PmeI PmlI PshAI PshBI Psp1406I PstI PvuI PvuII RcaI RsrII SacI SacII SalI SapI SbfI ScaI SexAI SfcI SfiI SfoI SgrAI SmaI SnaBI SpeI Sse8387I StuI StyI SwaI Tth111I Van91I XbaI XhoI XmaCI XmaI XmnI ################################### # ONE CUT ENZYMES ( 53 enzymes) # ################################### AciI 630 GCGG CCGC(-3/-1) AluI 624 AGCT AG^CT AocI 372 CCTAAGG CC^T[ACGT]AGG AvaII 1002 GGTCC G^G[AT]CC BbsI 775 GAAGAC GAAGAC(2/6) BfaI 145 CTAG C^TAG BpuAI 775 GAAGAC GAAGAC(2/6) BsaJI 644 CCACGG C^C[ACGT][ACGT]GG BsaWI 608 ACCGGA [AT]^CCGG[AT] BsgI 921 CTGCAC GTGCAG(16/14) BsiYI 326 CCGTTGATCGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG BslI 326 CCGTTGATCGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG BspLU11I 10 ACATGT A^CATGT BspMI 316 ACCTGC ACCTGC(4/8) BsrDI 321 CATTGC GCAATG(2/0) BssKI 33 CCTGG ^CC[ACGT]GG BstBI 772 TTCGAA TT^CGAA BstEII 588 GGTCACC G^GT[ACGT]ACC BstF5I 594 CATCC GGATG(2/0) BstNI 33 CCTGG CC^[AT]GG BstPI 588 GGTCACC G^GT[ACGT]ACC BstYI 100 GGATCT [GA]^GATC[CT] Bsu36I 372 CCTAAGG CC^T[ACGT]AGG Cfr13I 1002 GGTCC G^G[ACGT]CC DdeI 373 CTAAG C^T[ACGT]AG DraI 840 TTTAAA TTT^AAA DraII 1001 AGGTCCT [GA]G^G[ACGT]CC[CT] DsaI 644 CCACGG C^C[GA][CT]GG Eco81I 372 CCTAAGG CC^T[ACGT]AGG EcoO109I 1001 AGGTCCT [GA]G^G[ACGT]CC[CT] EcoO65I 588 GGTCACC G^GT[ACGT]ACC EcoRII 33 CCTGG ^CC[AT]GG EcoT22I 188 ATGCAT ATGCA^T FokI 594 CATCC GGATG(9/13) HapII 609 CCGG C^CGG HpaII 609 CCGG C^CGG MaeI 145 CTAG C^TAG MaeII 562 ACGT A^CGT MflI 100 GGATCT [GA]^GATC[CT] MspI 609 CCGG C^CGG MvaI 33 CCTGG CC^[AT]GG NsiI 188 ATGCAT ATGCA^T NspV 772 TTCGAA TT^CGAA PpuMI 1001 AGGTCCT [GA]G^G[AT]CC[CT] Sau96I 1002 GGTCC G^G[ACGT]CC ScrFI 33 CCTGG CC^[ACGT]GG SfuI 772 TTCGAA TT^CGAA SmlI 1006 CTTGAG C^T[CT][GA]AG SphI 759 GCATGC GCATG^C TaqI 773 TCGA T^CGA TspRI 924 CACTG CA[GC]TG[ACGT][ACGT]^ TthHB8I 773 TCGA T^CGA XhoII 100 GGATCT [GA]^GATC[CT] ################################### # TWO CUT ENZYMES ( 18 enzymes) # ################################### AflIII 10 ACATGT A^C[GA][CT]GT 562 ACGTGT BbvI 299 GCAGC GCAGC(8/12) 746 GCTGC BsmI 83 GCATTC GAATGC(1/-1) 307 GCATTC Bsp1407I 281 TGTACA T^GTACA 565 TGTACA BsrGI 281 TGTACA T^GTACA 565 TGTACA BsrI 236 ACTGG ACTGG(1/-1) 925 ACTGG Cac8I 123 GCAAGC GC[ACGT]^[ACGT]GC 759 GCATGC EarI 157 CTCTTC CTCTTC(1/4) 663 GAAGAG HphI 314 TCACC GGTGA(8/7) 590 TCACC Ksp632I 157 CTCTTC CTCTTC(1/4) 663 GAAGAG MslI 63 CATCTTTGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG 279 CATGTACATG NspI 10 ACATGT [GA]CATG^[CT] 759 GCATGC SfaNI 481 GATGC GCATC(5/9) 689 GCATC SspBI 281 TGTACA T^GTACA 565 TGTACA SspI 94 AATATT AAT^ATT 473 AATATT TseI 299 GCAGC G^C[AT]GC 746 GCTGC Tsp45I 168 GTCAC ^GT[GC]AC 589 GTCAC XcmI 410 CCATCTGGATATTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG 780 CCACAGCAATTCTGG ######################################### # 3 OR MORE CUT ENZYMES ( 21 enzymes) # ######################################### AccI AcsI AfaI AlwI ApoI DpnII Fnu4HI HinfI ItaI MaeIII MboI MboII MnlI MseI NdeII NlaIII RsaI Sau3AI TfiI Tru9I Tsp509I