(Circular) (Six-base) MAPSORT of: pFN21AB7141.seq Check: 5478 from: 1 to: 5716 pFN21AB7141 5716 bp With 182 enzymes: SgfI * December 28, 2011 15:26 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 2330 2330 Size: 5716 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3526 278 Size: 53 83 186 936 1990 2468 Fragments arranged by size: 2468 1990 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2355 2622 2981 3059 1937 Size: 418 267 359 78 4594 Fragments arranged by size: 4594 418 359 267 78 Acc65I G'GTAC_C Cuts at: 3037 3037 Size: 5716 AclI AA'CG_TT Cuts at: 1563 3843 4216 1563 Size: 2280 373 3063 Fragments arranged by size: 3063 2280 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2149 3779 5163 5431 1632 Size: 517 1630 1384 268 1917 Fragments arranged by size: 1917 1630 1384 517 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5519 AflIII A'CryG_T Cuts at: 1203 2015 2567 4615 5592 1203 Size: 812 552 2048 977 1327 Fragments arranged by size: 2048 1327 977 812 552 AgeI A'CCGG_T Cuts at: 4547 4547 Size: 5716 AhdI GACnn_n'nnGTC Cuts at: 4445 4445 Size: 5716 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 5716 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 5716 AlwNI CAG_nnn'CTG Cuts at: 3459 5031 3459 Size: 1572 4144 ApaLI G'TGCA_C Cuts at: 3773 4929 3773 Size: 1156 4560 ApoI r'AATT_y Cuts at: 1493 2154 2293 2921 3025 3251 3403 5512 1493 Size: 661 139 628 104 226 152 2109 1697 Fragments arranged by size: 2109 1697 661 628 226 152 139 104 AseI AT'TA_AT Cuts at: 160 1032 4270 160 Size: 872 3238 1606 Fragments arranged by size: 3238 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5716 AvaI C'yCGr_G Cuts at: 1870 1943 3041 1870 Size: 73 1098 4545 Fragments arranged by size: 4545 1098 73 BamHI G'GATC_C Cuts at: 3046 3413 3046 Size: 367 5349 BanI G'GyrC_C Cuts at: 618 943 1160 1787 3037 4492 618 Size: 325 217 627 1250 1455 1842 Fragments arranged by size: 1842 1455 1250 627 325 217 BanII G_rGCy'C Cuts at: 1391 3035 1391 Size: 1644 4072 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2787 928 Size: 971 888 3857 Fragments arranged by size: 3857 971 888 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 3907 3941 1903 Size: 34 1970 34 3678 Fragments arranged by size: 3678 1970 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3608 4818 3608 Size: 1210 4506 BclI T'GATC_A Cuts at: 1548 1548 Size: 5716 BfrBI ATG'CAT Cuts at: 2195 2195 Size: 5716 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 4327 136 Size: 107 122 71 631 605 2655 1525 Fragments arranged by size: 2655 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 5711 5711 Size: 5716 BlpI GC'TnA_GC Cuts at: 3133 3133 Size: 5716 Bme1580I G_kGCm'C Cuts at: 1790 3777 4933 1790 Size: 1987 1156 2573 Fragments arranged by size: 2573 1987 1156 BmgBI CAC'GTC Cuts at: 1318 2568 1318 Size: 1250 4466 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2940 3447 4405 Size: 416 53 83 399 24 1523 507 958 Cuts at: 4405 442 Size: 1753 Fragments arranged by size: 1753 1523 958 507 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5716 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 4376 1239 Size: 3137 2579 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 3032 3841 4706 5004 3032 Size: 809 865 298 3744 Fragments arranged by size: 3744 865 809 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 4379 882 Size: 617 2880 2219 Fragments arranged by size: 2880 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 3507 493 Size: 711 2303 2702 Fragments arranged by size: 2702 2303 711 BsaBI GATnn'nnATC Cuts at: 5710 5710 Size: 5716 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 3523 3905 4540 275 Size: 53 83 186 936 1990 382 635 1451 Fragments arranged by size: 1990 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 2613 4149 4547 4821 4968 5379 2613 Size: 1536 398 274 147 411 2950 Fragments arranged by size: 2950 1536 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2236 2266 1178 Size: 30 165 30 833 30 4628 Fragments arranged by size: 4628 833 165 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 1186 Size: 5716 BseYI C'CCAG_C Cuts at: 4919 5415 4919 Size: 496 5220 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2911 1163 Size: 573 1175 3968 Fragments arranged by size: 3968 1175 573 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3166 3927 4955 1540 Size: 275 186 1165 761 1028 2301 Fragments arranged by size: 2301 1165 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 3777 3862 4933 3777 Size: 85 1071 4560 Fragments arranged by size: 4560 1071 85 BsmI GAATG_Cn' Cuts at: 2088 2312 3227 3320 2088 Size: 224 915 93 4484 Fragments arranged by size: 4484 915 224 93 Bsp1286I G_dGCh'C Cuts at: 1391 1790 3035 3777 3862 4933 1391 Size: 399 1245 742 85 1071 2174 Fragments arranged by size: 2174 1245 1071 742 399 85 BspEI T'CCGG_A Cuts at: 5379 5379 Size: 5716 BspHI T'CATG_A Cuts at: 3605 5335 3605 Size: 1730 3986 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2330 3071 844 Size: 1486 741 3489 Fragments arranged by size: 3489 1486 741 BsrBI CCG'CTC Cuts at: 1394 1823 3603 1394 Size: 429 1780 3507 Fragments arranged by size: 3507 1780 429 BsrDI GCAATG_nn' Cuts at: 65 1248 2325 4211 4385 65 Size: 1183 1077 1886 174 1396 Fragments arranged by size: 1886 1396 1183 1077 174 BsrFI r'CCGG_y Cuts at: 1700 1810 4360 4547 1700 Size: 110 2550 187 2869 Fragments arranged by size: 2869 2550 187 110 BsrGI T'GTAC_A Cuts at: 96 2286 2570 96 Size: 2190 284 3242 Fragments arranged by size: 3242 2190 284 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 5716 BssSI C'ACGA_G Cuts at: 3776 4788 3776 Size: 1012 4704 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 5716 BstBI TT'CG_AA Cuts at: 2778 4520 2778 Size: 1742 3974 BstEII G'GTnAC_C Cuts at: 1184 2593 1184 Size: 1409 4307 BstYI r'GATC_y Cuts at: 1972 2105 3046 3413 3798 3815 5267 5353 5365 Size: 133 941 367 385 17 1452 86 12 Cuts at: 5365 5711 1972 Size: 346 1977 Fragments arranged by size: 1977 1452 941 385 367 346 133 86 17 12 Bsu36I CC'TnA_GG Cuts at: 2378 2378 Size: 5716 BtgI C'CryG_G Cuts at: 513 1065 2649 513 Size: 552 1584 3580 Fragments arranged by size: 3580 1584 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 2499 487 Size: 683 56 1273 3704 Fragments arranged by size: 3704 1273 683 56 BtsI GCAGTG_nn' Cuts at: 3232 4026 4046 3232 Size: 794 20 4902 Fragments arranged by size: 4902 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5716 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5681 DraI TTT'AAA Cuts at: 2847 3020 2847 Size: 173 5543 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4723 809 Size: 507 3407 1802 Fragments arranged by size: 3407 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 3163 4052 5681 Size: 54 1425 50 275 18 1333 889 1629 Cuts at: 5681 8 Size: 43 Fragments arranged by size: 1629 1425 1333 889 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3163 1537 Size: 275 1351 4090 Fragments arranged by size: 4090 1351 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2168 2663 3646 1353 Size: 815 495 983 3423 Fragments arranged by size: 3423 983 815 495 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4303 4677 4823 387 Size: 1064 138 2714 374 146 1280 Fragments arranged by size: 2714 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2149 3779 4376 5163 5431 1239 Size: 393 517 1630 597 787 268 1524 Fragments arranged by size: 1630 1524 787 597 517 393 268 EcoO109I rG'GnC_Cy Cuts at: 3007 3007 Size: 5716 EcoRI G'AATT_C Cuts at: 3025 3403 3025 Size: 378 5338 FspI TGC'GCA Cuts at: 4222 4222 Size: 5716 HaeII r_GCGC'y Cuts at: 1678 4863 1678 Size: 3185 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2236 2268 2382 2414 4357 Size: 32 135 32 664 32 114 32 1943 Cuts at: 4357 4389 4431 4463 1373 Size: 32 42 32 2626 Fragments arranged by size: 2626 1943 664 135 114 42 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3060 3306 669 Size: 988 281 1122 246 3079 Fragments arranged by size: 3079 1122 988 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 5716 HpaI GTT'AAC Cuts at: 3306 3306 Size: 5716 KpnI G_GTAC'C Cuts at: 3041 3041 Size: 5716 MfeI C'AATT_G Cuts at: 3315 3315 Size: 5716 MluI A'CGCG_T Cuts at: 5592 5592 Size: 5716 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 4830 5014 1305 Size: 3525 184 2007 Fragments arranged by size: 3525 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 3894 Fragments arranged by size: 3894 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2072 2288 3674 4033 4192 518 Size: 648 906 216 1386 359 159 2042 Fragments arranged by size: 2042 1386 906 648 359 216 159 MspA1I CmG'CkG Cuts at: 1606 3132 3809 4957 5202 1606 Size: 1526 677 1148 245 2120 Fragments arranged by size: 2120 1526 1148 677 245 NcoI C'CATG_G Cuts at: 513 1065 513 Size: 552 5164 NdeI CA'TA_TG Cuts at: 387 387 Size: 5716 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5716 NotI GC'GGCC_GC Cuts at: 3163 3163 Size: 5716 NsiI A_TGCA'T Cuts at: 2197 2197 Size: 5716 NspI r_CATG'y Cuts at: 1132 1229 2019 2768 3074 4619 1132 Size: 97 790 749 306 1545 2229 Fragments arranged by size: 2229 1545 790 749 306 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 5716 PciI A'CATG_T Cuts at: 2015 4615 2015 Size: 2600 3116 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 5716 PmeI GTTT'AAAC Cuts at: 3020 3020 Size: 5716 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3785 3817 5298 5330 3785 Size: 32 1481 32 4171 Fragments arranged by size: 4171 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 3007 3007 Size: 5716 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5716 PsiI TTA'TAA Cuts at: 3286 3286 Size: 5716 PstI C_TGCA'G Cuts at: 830 3068 830 Size: 2238 3478 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5716 SalI G'TCGA_C Cuts at: 1936 3058 1936 Size: 1122 4594 SbfI CC_TGCA'GG Cuts at: 3068 3068 Size: 5716 ScaI AGT'ACT Cuts at: 3964 5676 3964 Size: 1712 4004 SfcI C'TryA_G Cuts at: 826 1045 3064 4199 4880 5071 826 Size: 219 2019 1135 681 191 1471 Fragments arranged by size: 2019 1471 1135 681 219 191 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5716 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5716 SmaI CCC'GGG Cuts at: 1872 3043 1872 Size: 1171 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 3011 3820 4721 4983 820 Size: 197 926 1068 809 901 262 1553 Fragments arranged by size: 1553 1068 926 901 809 262 197 SnaBI TAC'GTA Cuts at: 493 3507 493 Size: 3014 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5716 SphI G_CATG'C Cuts at: 1132 2768 3074 1132 Size: 1636 306 3774 Fragments arranged by size: 3774 1636 306 SspI AAT'ATT Cuts at: 5 52 2101 2480 3640 5 Size: 47 2049 379 1160 2081 Fragments arranged by size: 2081 2049 1160 379 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 5716 StyI C'CwwG_G Cuts at: 513 1065 3155 513 Size: 552 2090 3074 Fragments arranged by size: 3074 2090 552 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 3743 3760 3913 4098 4543 325 Size: 1255 2163 17 153 185 445 1498 Fragments arranged by size: 2163 1498 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2286 2570 2871 Size: 274 80 33 51 1443 309 284 301 Cuts at: 2871 3962 5674 96 Size: 1091 1712 138 Fragments arranged by size: 1712 1443 1091 309 301 284 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 5676 5708 5676 Size: 32 5684 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 5716 XbaI T'CTAG_A Cuts at: 3052 3052 Size: 5716 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2422 2792 2422 Size: 370 5346 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5716 XmaI C'CCGG_G Cuts at: 1870 3041 1870 Size: 1171 4545 XmnI GAAnn'nnTTC Cuts at: 3845 3845 Size: 5716 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3524 276 Size: 53 83 186 936 1990 2468 Fragments arranged by size: 2468 1990 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NsiI NspI PasI PciI PflMI PmeI PpiI PpuMI PshAI PsiI PstI PvuI SalI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AfeI AloI ApaI AscI AvrII BaeI BbeI BbvCI BplI Bpu10I BsiWI BsmBI BstXI BstZ17I DraIII EcoICRI EcoNI EcoRV FalI FseI FspAI KasI NaeI NarI NgoMIV NruI PacI PfoI PmlI PspOMI PspXI PsrI PvuII RsrII SacI SacII SanDI SapI SexAI SfiI SfoI SrfI SwaI