################################## # NO CUT ENZYMES ( 146 enzymes) # ################################## AatII Acc65I AccI AccIII AclI AcsI AcyI AflII AflIII AgeI Alw44I AlwNI AocI Aor51HI ApaI ApaLI ApoI AscI AseI AsnI Asp718I AspI AviII AvrII BbeI BbrPI BbsI BbvCI BcgI BclI BfrI BlnI BlpI Bpu1102I BpuAI BsaAI BsaBI BsaHI BsaI BsaWI BseAI BsiEI BsiWI BsmBI BsmI Bsp1407I BspDI BspEI BspHI BspLU11I BspMI BsrBI BsrFI BsrGI BssHII Bst1107I BstAPI BstBI BstEII BstPI BstXI BstZ17I Bsu36I CelII CfoI Cfr10I ClaI CpoI DraI DraII DraIII DrdI EagI EclXI Eco47III Eco52I Eco81I EcoO109I EcoO65I EcoRI EcoRV EcoT22I FauI FbaI FseI FspI HaeII HhaI Hin1I HinP1I HincII HindII HindIII HpaI KasI KpnI KspI MamI MfeI MluI MroI MunI NaeI NarI NgoMIV NheI NotI NruI NsiI NspV PacI PflFI PflMI PinAI PmaCI PmeI PmlI PpuMI PshAI PshBI Psp1406I PstI PvuI RcaI RsrII SacII SalI SbfI ScaI SexAI SfiI SfoI SfuI SgrAI SmaI SnaBI SpeI Sse8387I SspBI SwaI Tth111I Van91I XbaI XcmI XmaCI XmaI ################################### # ONE CUT ENZYMES ( 34 enzymes) # ################################### AccII 927 CGCG CG^CG AfaI 870 GTAC GT^AC AhdI 601 GACATCTGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC Asp700I 528 GAACACCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC AspEI 601 GACATCTGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC BamHI 259 GGATCC G^GATCC BciVI 273 GGATAC GTATCC(6/5) BglI 26 GCCTGCCCGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC BglII 324 AGATCT A^GATCT BpmI 1252 CTCCAG CTGGAG(16/14) BsmAI 428 GAGAC GTCTC(1/5) BsrDI 827 GCAATG GCAATG(2/0) BsrI 971 ACTGG ACTGG(1/-1) BssSI 1240 CTCGTG CACGAG(-5/-1) BstUI 927 CGCG CG^CG DsaI 1226 CCATGG C^C[GA][CT]GG Eam1105I 601 GACATCTGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC EcoNI 1191 CCTAGCGGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG EcoT14I 1226 CCATGG C^C[AT][AT]GG HgaI 925 GACGC GACGC(5/10) HphI 471 GGTGA GGTGA(8/7) MvnI 927 CGCG CG^CG NcoI 1226 CCATGG C^CATGG NdeI 239 CATATG CA^TATG NspI 35 GCATGC [GA]CATG^[CT] PvuII 502 CAGCTG CAG^CTG RsaI 870 GTAC GT^AC SfaNI 1214 GCATC GCATC(5/9) SfcI 726 CTACAG C^T[GA][CT]AG SphI 35 GCATGC GCATG^C SspI 251 AATATT AAT^ATT StyI 1226 CCATGG C^C[AT][AT]GG Tsp45I 230 GTGAC ^GT[GC]AC XmnI 528 GAACACCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC ################################### # TWO CUT ENZYMES ( 21 enzymes) # ################################### AvaII 608 GGTCC G^G[AT]CC 1160 GGTCC BalI 498 TGGCCA TGG^CCA 939 TGGCCA BanI 541 GGCACC G^G[CT][GA]CC 558 GGCACC BcnI 12 CCCGG CC^[GC]GG 31 CCCGG BseRI 1106 CTCCTC GAGGAG(10/8) 1198 GAGGAG BsmFI 968 GGGAC GGGAC(10/14) 609 GTCCC HapII 13 CCGG C^CGG 32 CCGG HpaII 13 CCGG C^CGG 32 CCGG MaeII 446 ACGT A^CGT 712 ACGT MaeIII 230 GTGAC ^GT[ACGT]AC 244 GTAAC MluNI 498 TGGCCA TGG^CCA 939 TGGCCA MscI 498 TGGCCA TGG^CCA 939 TGGCCA MseI 373 TTAA T^TAA 434 TTAA MslI 531 CACCTTCGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG 1224 CACCATGGTG MspA1I 502 CAGCTG C[AC]G^C[GT]G 1246 CAGCGG MspI 13 CCGG C^CGG 32 CCGG NciI 12 CCCGG CC^[GC]GG 31 CCCGG PaeR7I 906 CTCGAG C^TCGAG 1009 CTCGAG StuI 755 AGGCCT AGG^CCT 1072 AGGCCT Tru9I 373 TTAA T^TAA 434 TTAA XhoI 906 CTCGAG C^TCGAG 1009 CTCGAG ######################################### # 3 OR MORE CUT ENZYMES ( 57 enzymes) # ######################################### AciI AluI AlwI AspHI AvaI BanII BbvI BfaI BmyI BsaJI BsgI BsiHKAI BsiYI BslI BsoBI Bsp1286I BssKI BstF5I BstNI BstYI Cac8I Cfr13I DdeI DpnII EaeI EarI EcoRII Fnu4HI FokI HaeIII HinfI ItaI Ksp632I MaeI MboI MboII MflI MnlI MvaI MwoI NdeII NlaIII NlaIV PleI SacI SapI Sau3AI Sau96I ScrFI SmlI TaqI TfiI TseI Tsp509I TspRI TthHB8I XhoII