(Circular) (Six-base) MAPSORT of: pFN21AE1320.seq Check: 7484 from: 1 to: 5998 pFN21AE1320 5998 bp With 182 enzymes: SgfI * August 16, 2010 14:47 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3808 278 Size: 53 83 186 936 2272 2468 Fragments arranged by size: 2468 2272 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3341 1937 Size: 1404 4594 Acc65I G'GTAC_C Cuts at: 3319 3319 Size: 5998 AclI AA'CG_TT Cuts at: 1563 4125 4498 1563 Size: 2562 373 3063 Fragments arranged by size: 3063 2562 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2205 2294 3014 4061 5445 5713 1632 Size: 573 89 720 1047 1384 268 1917 Fragments arranged by size: 1917 1384 1047 720 573 268 89 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5801 AflIII A'CryG_T Cuts at: 1203 4897 5874 1203 Size: 3694 977 1327 Fragments arranged by size: 3694 1327 977 AgeI A'CCGG_T Cuts at: 4829 4829 Size: 5998 AhdI GACnn_n'nnGTC Cuts at: 2611 4727 2611 Size: 2116 3882 AleI CACnn'nnGTG Cuts at: 1166 2540 3233 1166 Size: 1374 693 3931 Fragments arranged by size: 3931 1374 693 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 5998 AloI GAACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 3149 3181 3149 Size: 32 5966 AlwNI CAG_nnn'CTG Cuts at: 3741 5313 3741 Size: 1572 4426 ApaLI G'TGCA_C Cuts at: 4055 5211 4055 Size: 1156 4842 ApoI r'AATT_y Cuts at: 1493 3307 3533 3685 5794 1493 Size: 1814 226 152 2109 1697 Fragments arranged by size: 2109 1814 1697 226 152 AseI AT'TA_AT Cuts at: 160 1032 4552 160 Size: 872 3520 1606 Fragments arranged by size: 3520 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5998 AvaI C'yCGr_G Cuts at: 1870 1943 2567 2911 2953 3014 3323 1870 Size: 73 624 344 42 61 309 4545 Fragments arranged by size: 4545 624 344 309 73 61 42 BamHI G'GATC_C Cuts at: 2264 3328 3695 2264 Size: 1064 367 4567 Fragments arranged by size: 4567 1064 367 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2546 2563 3319 4774 618 Size: 325 217 627 759 17 756 1455 1842 Fragments arranged by size: 1842 1455 759 756 627 325 217 17 BanII G_rGCy'C Cuts at: 1391 2105 2865 2918 3028 3317 1391 Size: 714 760 53 110 289 4072 Fragments arranged by size: 4072 760 714 289 110 53 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 928 Size: 971 5027 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 4189 4223 1903 Size: 34 2252 34 3678 Fragments arranged by size: 3678 2252 34 34 BciVI GTATCCnnnnn_n' Cuts at: 2272 3890 5100 2272 Size: 1618 1210 3170 Fragments arranged by size: 3170 1618 1210 BclI T'GATC_A Cuts at: 1548 1548 Size: 5998 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2037 4609 136 Size: 107 122 71 631 605 365 2572 1525 Fragments arranged by size: 2572 1525 631 605 365 122 107 71 BglII A'GATC_T Cuts at: 2329 5993 2329 Size: 3664 2334 BlpI GC'TnA_GC Cuts at: 3415 3415 Size: 5998 Bme1580I G_kGCm'C Cuts at: 1790 2549 3215 4059 5215 1790 Size: 759 666 844 1156 2573 Fragments arranged by size: 2573 1156 844 759 666 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 5998 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3729 4687 442 Size: 416 53 83 399 24 2312 958 1753 Fragments arranged by size: 2312 1753 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5998 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3285 3285 Size: 5998 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 3242 4658 1239 Size: 2003 1416 2579 Fragments arranged by size: 2579 2003 1416 Bpu10I CC'TnA_GC Cuts at: 2799 2799 Size: 5998 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2729 4123 4988 5286 2729 Size: 1394 865 298 3441 Fragments arranged by size: 3441 1394 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 4661 882 Size: 617 3162 2219 Fragments arranged by size: 3162 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 3789 493 Size: 711 2585 2702 Fragments arranged by size: 2702 2585 711 BsaBI GATnn'nnATC Cuts at: 5992 5992 Size: 5998 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 3805 4187 4822 275 Size: 53 83 186 936 2272 382 635 1451 Fragments arranged by size: 2272 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 4431 4829 5103 5250 5661 4431 Size: 398 274 147 411 4768 Fragments arranged by size: 4768 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2900 2930 3194 3224 1178 Size: 30 165 30 1497 30 264 30 3952 Fragments arranged by size: 3952 1497 264 165 30 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 3102 3218 1186 Size: 1916 116 3966 Fragments arranged by size: 3966 1916 116 BseYI C'CCAG_C Cuts at: 3240 5201 5697 3240 Size: 1961 496 3541 Fragments arranged by size: 3541 1961 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2042 2654 3269 1163 Size: 573 306 612 615 3892 Fragments arranged by size: 3892 615 612 573 306 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3448 4209 5237 1540 Size: 275 186 1447 761 1028 2301 Fragments arranged by size: 2301 1447 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2865 2918 3028 4059 4144 5215 2865 Size: 53 110 1031 85 1071 3648 Fragments arranged by size: 3648 1071 1031 110 85 53 BsmI GAATG_Cn' Cuts at: 3509 3602 3509 Size: 93 5905 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2105 2549 2865 2918 3028 3215 3317 Size: 399 315 444 316 53 110 187 102 Cuts at: 3317 4059 4144 5215 1391 Size: 742 85 1071 2174 Fragments arranged by size: 2174 1071 742 444 399 316 315 187 110 102 85 53 BspEI T'CCGG_A Cuts at: 5661 5661 Size: 5998 BspHI T'CATG_A Cuts at: 3887 5617 3887 Size: 1730 4268 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 3353 844 Size: 2509 3489 BsrBI CCG'CTC Cuts at: 1394 1823 3885 1394 Size: 429 2062 3507 Fragments arranged by size: 3507 2062 429 BsrDI GCAATG_nn' Cuts at: 65 1248 2839 4493 4667 65 Size: 1183 1591 1654 174 1396 Fragments arranged by size: 1654 1591 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 4642 4829 1700 Size: 110 2832 187 2869 Fragments arranged by size: 2869 2832 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 5998 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 5998 BssSI C'ACGA_G Cuts at: 3245 4058 5070 3245 Size: 813 1012 4173 Fragments arranged by size: 4173 1012 813 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 5998 BstBI TT'CG_AA Cuts at: 4802 4802 Size: 5998 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 5998 BstYI r'GATC_y Cuts at: 1972 2264 2329 2979 3001 3328 3695 4080 4097 Size: 292 65 650 22 327 367 385 17 Cuts at: 4097 5549 5635 5647 5993 1972 Size: 1452 86 12 346 1977 Fragments arranged by size: 1977 1452 650 385 367 346 327 292 86 65 22 17 12 BtgI C'CryG_G Cuts at: 513 1065 2004 3231 513 Size: 552 939 1227 3280 Fragments arranged by size: 3280 1227 939 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5259 Fragments arranged by size: 5259 683 56 BtsI GCAGTG_nn' Cuts at: 2793 2968 3514 4308 4328 2793 Size: 175 546 794 20 4463 Fragments arranged by size: 4463 794 546 175 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5998 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 2700 2735 529 Size: 35 2136 35 3792 Fragments arranged by size: 3792 2136 35 35 DraI TTT'AAA Cuts at: 3302 3302 Size: 5998 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 5005 809 Size: 507 3689 1802 Fragments arranged by size: 3689 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2445 2503 2944 Size: 54 1425 50 275 18 615 58 441 Cuts at: 2944 3445 4334 5963 8 Size: 501 889 1629 43 Fragments arranged by size: 1629 1425 889 615 501 441 275 58 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3445 1537 Size: 275 1633 4090 Fragments arranged by size: 4090 1633 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2061 3033 3131 3168 3928 1353 Size: 708 972 98 37 760 3423 Fragments arranged by size: 3423 972 760 708 98 37 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2501 4585 4959 5105 387 Size: 1064 138 912 2084 374 146 1280 Fragments arranged by size: 2084 1280 1064 912 374 146 138 EcoICRI GAG'CTC Cuts at: 2863 2916 3026 2863 Size: 53 110 5835 Fragments arranged by size: 5835 110 53 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2205 2294 3014 3242 4061 4658 5445 Size: 393 573 89 720 228 819 597 787 Cuts at: 5445 5713 1239 Size: 268 1524 Fragments arranged by size: 1524 819 787 720 597 573 393 268 228 89 EcoNI CCTnn'n_nnAGG Cuts at: 3200 3200 Size: 5998 EcoRI G'AATT_C Cuts at: 3307 3685 3307 Size: 378 5620 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2122 2122 Size: 5998 FspI TGC'GCA Cuts at: 4504 4504 Size: 5998 HaeII r_GCGC'y Cuts at: 1678 5145 1678 Size: 3467 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2155 2187 2597 2629 2900 Size: 32 135 32 583 32 410 32 271 Cuts at: 2900 2932 3174 3206 4639 4671 4713 4745 1373 Size: 32 242 32 1433 32 42 32 2626 Fragments arranged by size: 2626 1433 583 410 271 242 135 42 32 32 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3342 3588 669 Size: 988 281 1404 246 3079 Fragments arranged by size: 3079 1404 988 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 5998 HpaI GTT'AAC Cuts at: 3588 3588 Size: 5998 KpnI G_GTAC'C Cuts at: 3323 3323 Size: 5998 MfeI C'AATT_G Cuts at: 3597 3597 Size: 5998 MluI A'CGCG_T Cuts at: 5874 5874 Size: 5998 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2700 2952 3249 5112 5296 1305 Size: 1395 252 297 1863 184 2007 Fragments arranged by size: 2007 1863 1395 297 252 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2505 2946 10 Size: 54 1425 343 673 441 3062 Fragments arranged by size: 3062 1425 673 441 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2540 3233 3956 4315 4474 518 Size: 648 1374 693 723 359 159 2042 Fragments arranged by size: 2042 1374 723 693 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2509 3253 3414 4091 5239 5484 1606 Size: 903 744 161 677 1148 245 2120 Fragments arranged by size: 2120 1148 903 744 677 245 161 NcoI C'CATG_G Cuts at: 513 1065 2004 3231 513 Size: 552 939 1227 3280 Fragments arranged by size: 3280 1227 939 552 NdeI CA'TA_TG Cuts at: 387 2245 387 Size: 1858 4140 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5998 NotI GC'GGCC_GC Cuts at: 3445 3445 Size: 5998 NspI r_CATG'y Cuts at: 1132 1229 2044 3356 4901 1132 Size: 97 815 1312 1545 2229 Fragments arranged by size: 2229 1545 1312 815 97 PasI CC'CwG_GG Cuts at: 1348 2617 1348 Size: 1269 4729 PciI A'CATG_T Cuts at: 4897 4897 Size: 5998 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 5998 PmeI GTTT'AAAC Cuts at: 3302 3302 Size: 5998 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4067 4099 5580 5612 4067 Size: 32 1481 32 4453 Fragments arranged by size: 4453 1481 32 32 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5998 PsiI TTA'TAA Cuts at: 3568 3568 Size: 5998 PspXI vC'TCGA_Gb Cuts at: 2911 2911 Size: 5998 PstI C_TGCA'G Cuts at: 830 3350 830 Size: 2520 3478 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5998 PvuII CAG'CTG Cuts at: 2509 2509 Size: 5998 SacI G_AGCT'C Cuts at: 2865 2918 3028 2865 Size: 53 110 5835 Fragments arranged by size: 5835 110 53 SalI G'TCGA_C Cuts at: 1936 3340 1936 Size: 1404 4594 SapI GCTCTTCn'nnn_ Cuts at: 2061 3033 3131 3168 2061 Size: 972 98 37 4891 Fragments arranged by size: 4891 972 98 37 SbfI CC_TGCA'GG Cuts at: 3350 3350 Size: 5998 ScaI AGT'ACT Cuts at: 4246 5958 4246 Size: 1712 4286 SfcI C'TryA_G Cuts at: 826 1045 2731 3346 4481 5162 5353 826 Size: 219 1686 615 1135 681 191 1471 Fragments arranged by size: 1686 1471 1135 681 615 219 191 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5998 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5998 SmaI CCC'GGG Cuts at: 1872 3325 1872 Size: 1453 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2744 2911 3014 4102 5003 5265 Size: 197 926 801 167 103 1088 901 262 Cuts at: 5265 820 Size: 1553 Fragments arranged by size: 1553 1088 926 901 801 262 197 167 103 SnaBI TAC'GTA Cuts at: 493 3789 493 Size: 3296 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5998 SphI G_CATG'C Cuts at: 1132 2044 3356 1132 Size: 912 1312 3774 Fragments arranged by size: 3774 1312 912 SspI AAT'ATT Cuts at: 5 52 2258 3922 5 Size: 47 2206 1664 2081 Fragments arranged by size: 2206 2081 1664 47 StuI AGG'CCT Cuts at: 1517 2762 3079 1517 Size: 1245 317 4436 Fragments arranged by size: 4436 1245 317 StyI C'CwwG_G Cuts at: 513 1065 2004 3231 3437 513 Size: 552 939 1227 206 3074 Fragments arranged by size: 3074 1227 939 552 206 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2534 2873 4025 4042 4195 4380 4825 Size: 1255 954 339 1152 17 153 185 445 Cuts at: 4825 325 Size: 1498 Fragments arranged by size: 1498 1255 1152 954 445 339 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 4244 5956 96 Size: 274 80 33 51 1443 2267 1712 138 Fragments arranged by size: 2267 1712 1443 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 3194 3226 5958 5990 3194 Size: 32 2732 32 3202 Fragments arranged by size: 3202 2732 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 5998 XbaI T'CTAG_A Cuts at: 3334 3334 Size: 5998 XhoI C'TCGA_G Cuts at: 1943 2911 3014 1943 Size: 968 103 4927 Fragments arranged by size: 4927 968 103 XmaI C'CCGG_G Cuts at: 1870 3323 1870 Size: 1453 4545 XmnI GAAnn'nnTTC Cuts at: 2537 4127 2537 Size: 1590 4408 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3806 276 Size: 53 83 186 936 2272 2468 Fragments arranged by size: 2468 2272 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AloI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PmeI PpiI PshAI PsiI PspXI PstI PvuI PvuII SacI SalI SapI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI ApaI AscI AvrII BaeI BbeI BbvCI BfrBI BsiWI BsmBI BstXI BstZ17I Bsu36I DraIII EcoO109I EcoRV FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PfoI PmlI PpuMI PspOMI PsrI RsrII SacII SanDI SexAI SfiI SfoI SrfI SwaI XcmI