(Linear) (Six-base) MAPSORT of: KIEE1320.seq Check: 5486 from: 1 to: 1293 KIEE1320.orf With 182 enzymes: SgfI * July 15, 2010 18:36 .. AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 0 200 289 1009 1293 Size: 200 89 720 284 Fragments arranged by size: 720 284 200 89 AhdI GACnn_n'nnGTC Cuts at: 0 606 1293 Size: 606 687 AleI CACnn'nnGTG Cuts at: 0 535 1228 1293 Size: 535 693 65 Fragments arranged by size: 693 535 65 AloI GAACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 0 1144 1176 1293 Size: 1144 32 117 Fragments arranged by size: 1144 117 32 AvaI C'yCGr_G Cuts at: 0 562 906 948 1009 1293 Size: 562 344 42 61 284 Fragments arranged by size: 562 344 284 61 42 BamHI G'GATC_C Cuts at: 0 259 1293 Size: 259 1034 BanI G'GyrC_C Cuts at: 0 541 558 1293 Size: 541 17 735 Fragments arranged by size: 735 541 17 BanII G_rGCy'C Cuts at: 0 100 860 913 1023 1293 Size: 100 760 53 110 270 Fragments arranged by size: 760 270 110 100 53 BciVI GTATCCnnnnn_n' Cuts at: 0 267 1293 Size: 267 1026 BglI GCCn_nnn'nGGC Cuts at: 0 32 1293 Size: 32 1261 BglII A'GATC_T Cuts at: 0 324 1293 Size: 324 969 Bme1580I G_kGCm'C Cuts at: 0 544 1210 1293 Size: 544 666 83 Fragments arranged by size: 666 544 83 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 0 1280 1293 Size: 1280 13 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 1237 1293 Size: 1237 56 Bpu10I CC'TnA_GC Cuts at: 0 794 1293 Size: 794 499 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 724 1293 Size: 724 569 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 0 895 925 1189 1219 1293 Size: 895 30 264 30 74 Fragments arranged by size: 895 264 74 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 0 1097 1213 1293 Size: 1097 116 80 Fragments arranged by size: 1097 116 80 BseYI C'CCAG_C Cuts at: 0 1235 1293 Size: 1235 58 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 0 37 649 1264 1293 Size: 37 612 615 29 Fragments arranged by size: 615 612 37 29 BsiHKAI G_wGCw'C Cuts at: 0 860 913 1023 1293 Size: 860 53 110 270 Fragments arranged by size: 860 270 110 53 Bsp1286I G_dGCh'C Cuts at: 0 100 544 860 913 1023 1210 1293 Size: 100 444 316 53 110 187 83 Fragments arranged by size: 444 316 187 110 100 83 53 BsrDI GCAATG_nn' Cuts at: 0 834 1293 Size: 834 459 BssSI C'ACGA_G Cuts at: 0 1240 1293 Size: 1240 53 BstYI r'GATC_y Cuts at: 0 259 324 974 996 1293 Size: 259 65 650 22 297 Fragments arranged by size: 650 297 259 65 22 BtgI C'CryG_G Cuts at: 0 1226 1293 Size: 1226 67 BtsI GCAGTG_nn' Cuts at: 0 788 963 1293 Size: 788 175 330 Fragments arranged by size: 788 330 175 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 0 695 730 1293 Size: 695 35 563 Fragments arranged by size: 695 563 35 EaeI y'GGCC_r Cuts at: 0 440 498 939 1293 Size: 440 58 441 354 Fragments arranged by size: 441 440 354 58 EarI CTCTTCn'nnn_ Cuts at: 0 56 1028 1126 1163 1293 Size: 56 972 98 37 130 Fragments arranged by size: 972 130 98 56 37 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 0 496 1293 Size: 496 797 EcoICRI GAG'CTC Cuts at: 0 858 911 1021 1293 Size: 858 53 110 272 Fragments arranged by size: 858 272 110 53 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 0 200 289 1009 1237 1293 Size: 200 89 720 228 56 Fragments arranged by size: 720 228 200 89 56 EcoNI CCTnn'n_nnAGG Cuts at: 0 1195 1293 Size: 1195 98 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 0 117 1293 Size: 117 1176 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 0 150 182 592 624 895 927 1169 1201 Size: 150 32 410 32 271 32 242 32 Cuts at: 1201 1293 Size: 92 Fragments arranged by size: 410 271 242 150 92 32 32 32 32 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 0 695 947 1244 1293 Size: 695 252 297 49 Fragments arranged by size: 695 297 252 49 MscI TGG'CCA Cuts at: 0 500 941 1293 Size: 500 441 352 Fragments arranged by size: 500 441 352 MslI CAynn'nnrTG Cuts at: 0 535 1228 1293 Size: 535 693 65 Fragments arranged by size: 693 535 65 MspA1I CmG'CkG Cuts at: 0 504 1248 1293 Size: 504 744 45 Fragments arranged by size: 744 504 45 NcoI C'CATG_G Cuts at: 0 1226 1293 Size: 1226 67 NdeI CA'TA_TG Cuts at: 0 240 1293 Size: 240 1053 NspI r_CATG'y Cuts at: 0 39 1293 Size: 39 1254 PasI CC'CwG_GG Cuts at: 0 612 1293 Size: 612 681 PspXI vC'TCGA_Gb Cuts at: 0 906 1293 Size: 906 387 PvuII CAG'CTG Cuts at: 0 504 1293 Size: 504 789 SacI G_AGCT'C Cuts at: 0 860 913 1023 1293 Size: 860 53 110 270 Fragments arranged by size: 860 270 110 53 SapI GCTCTTCn'nnn_ Cuts at: 0 56 1028 1126 1163 1293 Size: 56 972 98 37 130 Fragments arranged by size: 972 130 98 56 37 SfcI C'TryA_G Cuts at: 0 726 1293 Size: 726 567 SmlI C'TyrA_G Cuts at: 0 739 906 1009 1293 Size: 739 167 103 284 Fragments arranged by size: 739 284 167 103 SphI G_CATG'C Cuts at: 0 39 1293 Size: 39 1254 SspI AAT'ATT Cuts at: 0 253 1293 Size: 253 1040 StuI AGG'CCT Cuts at: 0 757 1074 1293 Size: 757 317 219 Fragments arranged by size: 757 317 219 StyI C'CwwG_G Cuts at: 0 1226 1293 Size: 1226 67 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 0 529 868 1293 Size: 529 339 425 Fragments arranged by size: 529 425 339 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 0 1189 1221 1293 Size: 1189 32 72 Fragments arranged by size: 1189 72 32 XhoI C'TCGA_G Cuts at: 0 906 1009 1293 Size: 906 103 284 Fragments arranged by size: 906 284 103 XmnI GAAnn'nnTTC Cuts at: 0 532 1293 Size: 532 761 Enzymes that do cut: AcuI AhdI AleI AloI AvaI BamHI BanI BanII BciVI BglI BglII Bme1580I BplI BpmI Bpu10I BpuEI BsaXI BseRI BseYI BsgI BsiHKAI Bsp1286I BsrDI BssSI BstYI BtgI BtsI CspCI EaeI EarI EciI EcoICRI Eco57MI EcoNI FalI Hin4I MmeI MscI MslI MspA1I NcoI NdeI NspI PasI PspXI PvuII SacI SapI SfcI SmlI SphI SspI StuI StyI TaqII TstI XhoI XmnI Enzymes that do not cut: AarI AatII AccI Acc65I AclI AfeI AflII AflIII AgeI AlfI AlwNI ApaI ApaLI ApoI AscI AseI AsiSI AvrII BaeI BbeI BbsI BbvCI BcgI BclI BfrBI BlpI BmgBI BmrI BmtI BsaI BsaAI BsaBI BsaHI BsaWI BsiEI BsiWI BsmI BsmBI BspEI BspHI BspMI BsrBI BsrFI BsrGI BssHII BstAPI BstBI BstEII BstXI BstZ17I Bsu36I BtgZI ClaI DraI DraIII DrdI EagI EcoO109I EcoRI EcoRV FseI FspI FspAI HaeII HincII HindIII HpaI KasI KpnI MfeI MluI NaeI NarI NgoMIV NheI NotI NruI NsiI PacI PciI PflMI PfoI PmeI PmlI PpiI PpuMI PshAI PsiI PspOMI PsrI PstI PvuI RsrII SacII SalI SanDI SbfI ScaI SexAI SfiI SfoI SgfI SgrAI SmaI SnaBI SpeI SrfI SwaI TatI Tth111I XbaI XcmI XmaI ZraI