################################## # NO CUT ENZYMES ( 136 enzymes) # ################################## AatII Acc65I AccIII AclI AflIII AhdI Alw44I AlwNI AocI Aor51HI ApaI ApaLI AscI Asp718I AspEI AspI AviII AvrII BanI BbeI BbrPI BbsI BbvCI BcgI BclI BfaI BglI BglII BlnI BlpI Bpu1102I BpuAI BsaAI BsaBI BseAI BsiEI BsiWI BsmI Bsp1407I BspDI BspEI BspHI BspLU11I BsrDI BsrGI BssHII BssSI BstBI BstEII BstPI BstXI Bsu36I CelII ClaI CpoI DraI DraIII EagI Eam1105I EclXI Eco47III Eco52I Eco81I EcoNI EcoO65I EcoRI EcoRV EcoT22I FbaI FseI FspI HaeII HindIII HinfI HpaI KasI KpnI KspI MaeI MamI MfeI MluI MroI MslI MunI NaeI NarI NdeI NgoMIV NheI NotI NruI NsiI NspV PacI PaeR7I PflFI PflMI PleI PmaCI PmeI PmlI PpuMI PshAI Psp1406I PvuI PvuII RcaI RsrII SacII SalI SapI SbfI ScaI SexAI SfiI SfoI SfuI SgrAI SmaI SnaBI SpeI SphI Sse8387I SspBI SspI StuI SwaI TfiI Tth111I Van91I XbaI XcmI XhoI XmaCI XmaI ################################### # ONE CUT ENZYMES ( 50 enzymes) # ################################### AccII 63 CGCG CG^CG AcyI 1051 GGCGTC G[GA]^CG[CT]C AfaI 319 GTAC GT^AC AgeI 521 ACCGGT A^CCGGT AseI 690 ATTAAT AT^TAAT AsnI 690 ATTAAT AT^TAAT Asp700I 1038 GAAGCACTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC AspHI 1026 GAGCTC G[AT]GC[AT]^C AvaI 1118 CCCGAG C^[CT]CG[GA]G AvaII 162 GGACC G^G[AT]CC BalI 137 TGGCCA TGG^CCA BamHI 120 GGATCC G^GATCC BciVI 807 GTATCC GTATCC(6/5) BcnI 252 CCGGG CC^[GC]GG BpmI 81 CTCCAG CTGGAG(16/14) BsaHI 1051 GGCGTC G[GA]^CG[CT]C BsaI 490 GAGACC GGTCTC(1/5) BsaWI 521 ACCGGT [AT]^CCGG[AT] BsgI 203 GTGCAG GTGCAG(16/14) BsiHKAI 1026 GAGCTC G[AT]GC[AT]^C BsmBI 1018 GAGACG CGTCTC(1/5) BsoBI 1118 CCCGAG C^[CT]CG[GA]G BspMI 762 GCAGGT ACCTGC(4/8) BsrFI 521 ACCGGT [GA]^CCGG[CT] BstAPI 196 GCAGTCTGTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC BstUI 63 CGCG CG^CG Cfr10I 521 ACCGGT [GA]^CCGG[CT] DdeI 322 CTGAG C^T[ACGT]AG DraII 704 AGGCCCT [GA]G^G[ACGT]CC[CT] DrdI 732 GACTATTGTGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC EaeI 137 TGGCCA [CT]^GGCC[GA] EcoO109I 704 AGGCCCT [GA]G^G[ACGT]CC[CT] HgaI 1052 GCGTC GACGC(5/10) Hin1I 1051 GGCGTC G[GA]^CG[CT]C HincII 865 GTTGAC GT[CT]^[GA]AC HindII 865 GTTGAC GT[CT]^[GA]AC MluNI 137 TGGCCA TGG^CCA MscI 137 TGGCCA TGG^CCA MvnI 63 CGCG CG^CG NciI 252 CCGGG CC^[GC]GG NspI 758 ACATGC [GA]CATG^[CT] PinAI 521 ACCGGT A^CCGGT PshBI 690 ATTAAT AT^TAAT PstI 418 CTGCAG CTGCA^G RsaI 319 GTAC GT^AC SacI 1026 GAGCTC GAGCT^C SfcI 418 CTGCAG C^T[GA][CT]AG TaqI 146 TCGA T^CGA TthHB8I 146 TCGA T^CGA XmnI 1038 GAAGCACTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC ################################### # TWO CUT ENZYMES ( 26 enzymes) # ################################### AccI 754 GTATAC GT^[AC][GT]AC 890 GTATAC AcsI 674 AAATTC [GA]^AATT[CT] 877 GAATTT AflII 381 CTTAAG C^TTAAG 709 CTTAAG ApoI 674 AAATTC [GA]^AATT[CT] 877 GAATTT BanII 1026 GAGCTC G[GA]GC[CT]^C 1174 GGGCTC BfrI 381 CTTAAG C^TTAAG 709 CTTAAG BmyI 1026 GAGCTC G[AGT]GC[ACT]^C 1174 GGGCTC BseRI 1160 CTCCTC GAGGAG(10/8) 1177 CTCCTC Bsp1286I 1026 GAGCTC G[AGT]GC[ACT]^C 1174 GGGCTC Bst1107I 754 GTATAC GTA^TAC 890 GTATAC BstYI 120 GGATCC [GA]^GATC[CT] 846 GGATCT BstZ17I 754 GTATAC GTA^TAC 890 GTATAC CfoI 12 GCGC GCG^C 62 GCGC DsaI 683 CCATGG C^C[GA][CT]GG 1004 CCATGG EarI 413 CTCTTC CTCTTC(1/4) 87 GAAGAG HhaI 12 GCGC GCG^C 62 GCGC HinP1I 12 GCGC G^CGC 62 GCGC HphI 680 TCACC GGTGA(8/7) 1181 TCACC Ksp632I 413 CTCTTC CTCTTC(1/4) 87 GAAGAG MaeII 101 ACGT A^CGT 271 ACGT MaeIII 103 GTGAC ^GT[ACGT]AC 646 GTGAC MflI 120 GGATCC [GA]^GATC[CT] 846 GGATCT MspA1I 310 CCGCTG C[AC]G^C[GT]G 1212 CAGCGG NcoI 683 CCATGG C^CATGG 1004 CCATGG Tsp45I 103 GTGAC ^GT[GC]AC 646 GTGAC XhoII 120 GGATCC [GA]^GATC[CT] 846 GGATCT ######################################### # 3 OR MORE CUT ENZYMES ( 46 enzymes) # ######################################### AciI AluI AlwI BbvI BsaJI BsiYI BslI BsmAI BsmFI BsrBI BsrI BssKI BstF5I BstNI Cac8I Cfr13I DpnII EcoRII EcoT14I FauI Fnu4HI FokI HaeIII HapII HpaII ItaI MboI MboII MnlI MseI MspI MvaI MwoI NdeII NlaIII NlaIV Sau3AI Sau96I ScrFI SfaNI SmlI StyI Tru9I TseI Tsp509I TspRI