(Circular) (Six-base) MAPSORT of: pFN21AE1359.seq Check: 1879 from: 1 to: 5935 pFN21AE1359 5935 bp With 182 enzymes: SgfI * August 16, 2010 14:45 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3745 278 Size: 53 83 186 936 2209 2468 Fragments arranged by size: 2468 2209 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2760 2896 3278 1937 Size: 823 136 382 4594 Fragments arranged by size: 4594 823 382 136 Acc65I G'GTAC_C Cuts at: 3256 3256 Size: 5935 AclI AA'CG_TT Cuts at: 1563 4062 4435 1563 Size: 2499 373 3063 Fragments arranged by size: 3063 2499 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 3034 3062 3998 5382 5650 1632 Size: 1402 28 936 1384 268 1917 Fragments arranged by size: 1917 1402 1384 936 268 28 AflII C'TTAA_G Cuts at: 820 1017 2386 2714 820 Size: 197 1369 328 4041 Fragments arranged by size: 4041 1369 328 197 AflIII A'CryG_T Cuts at: 1203 4834 5811 1203 Size: 3631 977 1327 Fragments arranged by size: 3631 1327 977 AgeI A'CCGG_T Cuts at: 2526 4766 2526 Size: 2240 3695 AhdI GACnn_n'nnGTC Cuts at: 4664 4664 Size: 5935 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 5935 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 2233 2658 1184 Size: 1049 425 4461 Fragments arranged by size: 4461 1049 425 AlwNI CAG_nnn'CTG Cuts at: 3678 5250 3678 Size: 1572 4363 ApaLI G'TGCA_C Cuts at: 3992 5148 3992 Size: 1156 4779 ApoI r'AATT_y Cuts at: 1493 2679 2882 3244 3470 3622 5731 1493 Size: 1186 203 362 226 152 2109 1697 Fragments arranged by size: 2109 1697 1186 362 226 203 152 AseI AT'TA_AT Cuts at: 160 1032 2696 4489 160 Size: 872 1664 1793 1606 Fragments arranged by size: 1793 1664 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5935 AvaI C'yCGr_G Cuts at: 1870 1943 3123 3260 1870 Size: 73 1180 137 4545 Fragments arranged by size: 4545 1180 137 73 BamHI G'GATC_C Cuts at: 2125 3265 3632 2125 Size: 1140 367 4428 Fragments arranged by size: 4428 1140 367 BanI G'GyrC_C Cuts at: 618 943 1160 1787 3256 4711 618 Size: 325 217 627 1469 1455 1842 Fragments arranged by size: 1842 1469 1455 627 325 217 BanII G_rGCy'C Cuts at: 1391 3035 3183 3254 1391 Size: 1644 148 71 4072 Fragments arranged by size: 4072 1644 148 71 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 928 Size: 971 4964 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 1987 2021 4126 4160 1903 Size: 34 50 34 2105 34 3678 Fragments arranged by size: 3678 2105 50 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 2823 3827 5037 2823 Size: 1004 1210 3721 Fragments arranged by size: 3721 1210 1004 BclI T'GATC_A Cuts at: 1548 1548 Size: 5935 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 4546 136 Size: 107 122 71 631 605 2874 1525 Fragments arranged by size: 2874 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 5930 5930 Size: 5935 BlpI GC'TnA_GC Cuts at: 3352 3352 Size: 5935 Bme1580I G_kGCm'C Cuts at: 1790 3996 5152 1790 Size: 2206 1156 2573 Fragments arranged by size: 2573 2206 1156 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 5935 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3168 3666 4624 Size: 416 53 83 399 24 1751 498 958 Cuts at: 4624 442 Size: 1753 Fragments arranged by size: 1753 1751 958 498 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5935 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2071 4595 1239 Size: 832 2524 2579 Fragments arranged by size: 2579 2524 832 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2426 2630 4060 4925 5223 2426 Size: 204 1430 865 298 3138 Fragments arranged by size: 3138 1430 865 298 204 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2489 4598 882 Size: 617 990 2109 2219 Fragments arranged by size: 2219 2109 990 617 BsaAI yAC'GTr Cuts at: 493 1204 3726 493 Size: 711 2522 2702 Fragments arranged by size: 2702 2522 711 BsaBI GATnn'nnATC Cuts at: 5929 5929 Size: 5935 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 3057 3742 4124 4759 Size: 53 83 186 936 1524 685 382 635 Cuts at: 4759 275 Size: 1451 Fragments arranged by size: 1524 1451 936 685 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 2526 4368 4766 5040 5187 5598 2526 Size: 1842 398 274 147 411 2863 Fragments arranged by size: 2863 1842 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2204 2234 1178 Size: 30 165 30 801 30 4879 Fragments arranged by size: 4879 801 165 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 3156 3173 1186 Size: 1970 17 3948 Fragments arranged by size: 3948 1970 17 BseYI C'CCAG_C Cuts at: 2331 2817 5138 5634 2331 Size: 486 2321 496 2632 Fragments arranged by size: 2632 2321 496 486 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2229 1163 Size: 573 493 4869 Fragments arranged by size: 4869 573 493 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3385 4146 5174 1540 Size: 275 186 1384 761 1028 2301 Fragments arranged by size: 2301 1384 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 3035 3996 4081 5152 3035 Size: 961 85 1071 3818 Fragments arranged by size: 3818 1071 961 85 BsmI GAATG_Cn' Cuts at: 3446 3539 3446 Size: 93 5842 BsmBI CGTCTCn'nnnn_ Cuts at: 3017 3017 Size: 5935 Bsp1286I G_dGCh'C Cuts at: 1391 1790 3035 3183 3254 3996 4081 5152 1391 Size: 399 1245 148 71 742 85 1071 2174 Fragments arranged by size: 2174 1245 1071 742 399 148 85 71 BspEI T'CCGG_A Cuts at: 5598 5598 Size: 5935 BspHI T'CATG_A Cuts at: 3824 5554 3824 Size: 1730 4205 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2758 3290 844 Size: 1914 532 3489 Fragments arranged by size: 3489 1914 532 BsrBI CCG'CTC Cuts at: 1394 1823 2026 2041 3201 3822 1394 Size: 429 203 15 1160 621 3507 Fragments arranged by size: 3507 1160 621 429 203 15 BsrDI GCAATG_nn' Cuts at: 65 1248 4430 4604 65 Size: 1183 3182 174 1396 Fragments arranged by size: 3182 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2526 4579 4766 1700 Size: 110 716 2053 187 2869 Fragments arranged by size: 2869 2053 716 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 5935 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 5935 BssSI C'ACGA_G Cuts at: 3995 5007 3995 Size: 1012 4923 BstAPI GCAn_nnn'nTGC Cuts at: 794 2207 794 Size: 1413 4522 BstBI TT'CG_AA Cuts at: 4739 4739 Size: 5935 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 5935 BstYI r'GATC_y Cuts at: 1972 2125 2851 3265 3632 4017 4034 5486 5572 Size: 153 726 414 367 385 17 1452 86 Cuts at: 5572 5584 5930 1972 Size: 12 346 1977 Fragments arranged by size: 1977 1452 726 414 385 367 346 153 86 17 12 BstZ17I GTA'TAC Cuts at: 2761 2897 2761 Size: 136 5799 BtgI C'CryG_G Cuts at: 513 1065 2688 3009 513 Size: 552 1623 321 3439 Fragments arranged by size: 3439 1623 552 321 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5196 Fragments arranged by size: 5196 683 56 BtsI GCAGTG_nn' Cuts at: 3451 4245 4265 3451 Size: 794 20 5121 Fragments arranged by size: 5121 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5935 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5900 DraI TTT'AAA Cuts at: 3239 3239 Size: 5935 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 2743 4942 809 Size: 507 1427 2199 1802 Fragments arranged by size: 2199 1802 1427 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2142 3382 4271 Size: 54 1425 50 275 18 312 1240 889 Cuts at: 4271 5900 8 Size: 1629 43 Fragments arranged by size: 1629 1425 1240 889 312 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3382 1537 Size: 275 1570 4090 Fragments arranged by size: 4090 1570 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2087 2424 3865 1353 Size: 734 337 1441 3423 Fragments arranged by size: 3423 1441 734 337 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4522 4896 5042 387 Size: 1064 138 2933 374 146 1280 Fragments arranged by size: 2933 1280 1064 374 146 138 EcoICRI GAG'CTC Cuts at: 3033 3033 Size: 5935 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2071 3034 3062 3998 4595 5382 5650 Size: 393 439 963 28 936 597 787 268 Cuts at: 5650 1239 Size: 1524 Fragments arranged by size: 1524 963 936 787 597 439 393 268 28 EcoO109I rG'GnC_Cy Cuts at: 2710 2710 Size: 5935 EcoRI G'AATT_C Cuts at: 3244 3622 3244 Size: 378 5557 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2401 2401 Size: 5935 FspI TGC'GCA Cuts at: 4441 4441 Size: 5935 HaeII r_GCGC'y Cuts at: 1678 5082 1678 Size: 3404 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2204 2236 2334 2366 4576 Size: 32 135 32 632 32 98 32 2210 Cuts at: 4576 4608 4650 4682 1373 Size: 32 42 32 2626 Fragments arranged by size: 2626 2210 632 135 98 42 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2872 3279 3525 669 Size: 988 281 934 407 246 3079 Fragments arranged by size: 3079 988 934 407 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 5935 HpaI GTT'AAC Cuts at: 3525 3525 Size: 5935 KpnI G_GTAC'C Cuts at: 3260 3260 Size: 5935 MfeI C'AATT_G Cuts at: 3534 3534 Size: 5935 MluI A'CGCG_T Cuts at: 5811 5811 Size: 5935 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2829 5049 5233 1305 Size: 1524 2220 184 2007 Fragments arranged by size: 2220 2007 1524 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2144 10 Size: 54 1425 343 312 3801 Fragments arranged by size: 3801 1425 343 312 54 MslI CAynn'nnrTG Cuts at: 518 1166 3893 4252 4411 518 Size: 648 2727 359 159 2042 Fragments arranged by size: 2727 2042 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2317 3219 3351 4028 5176 5421 1606 Size: 711 902 132 677 1148 245 2120 Fragments arranged by size: 2120 1148 902 711 677 245 132 NcoI C'CATG_G Cuts at: 513 1065 2688 3009 513 Size: 552 1623 321 3439 Fragments arranged by size: 3439 1623 552 321 NdeI CA'TA_TG Cuts at: 387 387 Size: 5935 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5935 NotI GC'GGCC_GC Cuts at: 3382 3382 Size: 5935 NspI r_CATG'y Cuts at: 1132 1229 2767 3293 4838 1132 Size: 97 1538 526 1545 2229 Fragments arranged by size: 2229 1545 1538 526 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 5935 PciI A'CATG_T Cuts at: 4834 4834 Size: 5935 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 5935 PfoI T'CCnGG_A Cuts at: 2087 2862 2087 Size: 775 5160 PmeI GTTT'AAAC Cuts at: 3239 3239 Size: 5935 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4004 4036 5517 5549 4004 Size: 32 1481 32 4390 Fragments arranged by size: 4390 1481 32 32 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5935 PsiI TTA'TAA Cuts at: 3017 3505 3017 Size: 488 5447 PstI C_TGCA'G Cuts at: 830 2427 3287 830 Size: 1597 860 3478 Fragments arranged by size: 3478 1597 860 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5935 SacI G_AGCT'C Cuts at: 3035 3035 Size: 5935 SalI G'TCGA_C Cuts at: 1936 3277 1936 Size: 1341 4594 SbfI CC_TGCA'GG Cuts at: 3287 3287 Size: 5935 ScaI AGT'ACT Cuts at: 4183 5895 4183 Size: 1712 4223 SfcI C'TryA_G Cuts at: 826 1045 2423 3283 4418 5099 5290 826 Size: 219 1378 860 1135 681 191 1471 Fragments arranged by size: 1471 1378 1135 860 681 219 191 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5935 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5935 SmaI CCC'GGG Cuts at: 1872 3262 1872 Size: 1390 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2386 2405 2609 2714 4039 4940 Size: 197 926 443 19 204 105 1325 901 Cuts at: 4940 5202 820 Size: 262 1553 Fragments arranged by size: 1553 1325 926 901 443 262 204 197 105 19 SnaBI TAC'GTA Cuts at: 493 3726 493 Size: 3233 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5935 SphI G_CATG'C Cuts at: 1132 3293 1132 Size: 2161 3774 SspI AAT'ATT Cuts at: 5 52 3859 5 Size: 47 3807 2081 Fragments arranged by size: 3807 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 5935 StyI C'CwwG_G Cuts at: 513 1065 2186 2688 3009 3374 513 Size: 552 1121 502 321 365 3074 Fragments arranged by size: 3074 1121 552 502 365 321 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 3962 3979 4132 4317 4762 325 Size: 1255 2382 17 153 185 445 1498 Fragments arranged by size: 2382 1498 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2323 4181 5893 Size: 274 80 33 51 1443 346 1858 1712 Cuts at: 5893 96 Size: 138 Fragments arranged by size: 1858 1712 1443 346 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 5895 5927 5895 Size: 32 5903 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 5935 XbaI T'CTAG_A Cuts at: 3271 3271 Size: 5935 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5935 XmaI C'CCGG_G Cuts at: 1870 3260 1870 Size: 1390 4545 XmnI GAAnn'nnTTC Cuts at: 3047 4064 3047 Size: 1017 4918 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3743 276 Size: 53 83 186 936 2209 2468 Fragments arranged by size: 2468 2209 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BstZ17I BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PfoI PmeI PpiI PshAI PsiI PstI PvuI SacI SalI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI ApaI AscI AvrII BaeI BbeI BbvCI BfrBI BplI Bpu10I BsiWI BstXI Bsu36I DraIII EcoNI EcoRV FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PmlI PpuMI PspOMI PspXI PsrI PvuII RsrII SacII SanDI SapI SexAI SfiI SfoI SrfI SwaI XcmI