Restriction map for
pFN21AE2155
(Linear) (Six-base) MAPSORT of: KIEE2155.seq Check: 9849 from: 1 to: 1134
KIEE2155.orf
With 182 enzymes: SgfI *
August 25, 2016 10:58 ..
AatII G_ACGT'C
Cuts at: 0 929 1134
Size: 929 205
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 405 1134
Size: 405 729
AleI CACnn'nnGTG
Cuts at: 0 112 1134
Size: 112 1022
BanII G_rGCy'C
Cuts at: 0 581 899 1134
Size: 581 318 235
Fragments arranged by size:
581 318 235
BbsI GAAGACnn'nnnn_
Cuts at: 0 360 804 813 1058 1134
Size: 360 444 9 245 76
Fragments arranged by size:
444 360 245 76 9
BbvCI CC'TCA_GC
Cuts at: 0 1039 1134
Size: 1039 95
BciVI GTATCCnnnnn_n'
Cuts at: 0 542 1134
Size: 542 592
BclI T'GATC_A
Cuts at: 0 791 1134
Size: 791 343
BglI GCCn_nnn'nGGC
Cuts at: 0 221 1134
Size: 221 913
Bme1580I G_kGCm'C
Cuts at: 0 507 1134
Size: 507 627
BmgBI CAC'GTC
Cuts at: 0 156 1134
Size: 156 978
BmrI ACTGGGnnnn_n'
Cuts at: 0 229 1134
Size: 229 905
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 580 736 1134
Size: 580 156 398
Fragments arranged by size:
580 398 156
Bpu10I CC'TnA_GC
Cuts at: 0 1039 1134
Size: 1039 95
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 250 1134
Size: 250 884
BsaI GGTCTCn'nnnn_
Cuts at: 0 1105 1134
Size: 1105 29
BsaAI yAC'GTr
Cuts at: 0 465 1134
Size: 465 669
BsaBI GATnn'nnATC
Cuts at: 0 685 1134
Size: 685 449
BsaHI Gr'CG_yC
Cuts at: 0 926 989 1134
Size: 926 63 145
Fragments arranged by size:
926 145 63
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 594 624 1134
Size: 594 30 510
Fragments arranged by size:
594 510 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 615 1080 1134
Size: 615 465 54
Fragments arranged by size:
615 465 54
BsiHKAI G_wGCw'C
Cuts at: 0 145 566 581 899 1134
Size: 145 421 15 318 235
Fragments arranged by size:
421 318 235 145 15
Bsp1286I G_dGCh'C
Cuts at: 0 145 507 566 581 899 1134
Size: 145 362 59 15 318 235
Fragments arranged by size:
362 318 235 145 59 15
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 296 1134
Size: 296 838
BsrDI GCAATG_nn'
Cuts at: 0 231 1134
Size: 231 903
BsrGI T'GTAC_A
Cuts at: 0 584 1134
Size: 584 550
BstAPI GCAn_nnn'nTGC
Cuts at: 0 621 1134
Size: 621 513
BstXI CCAn_nnnn'nTGG
Cuts at: 0 672 1101 1134
Size: 672 429 33
Fragments arranged by size:
672 429 33
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 634 639 780 900 1134
Size: 634 5 141 120 234
Fragments arranged by size:
634 234 141 120 5
BtsI GCAGTG_nn'
Cuts at: 0 615 1066 1134
Size: 615 451 68
Fragments arranged by size:
615 451 68
DraI TTT'AAA
Cuts at: 0 168 1134
Size: 168 966
EaeI y'GGCC_r
Cuts at: 0 353 467 710 863 1134
Size: 353 114 243 153 271
Fragments arranged by size:
353 271 243 153 114
EarI CTCTTCn'nnn_
Cuts at: 0 322 1015 1024 1134
Size: 322 693 9 110
Fragments arranged by size:
693 322 110 9
EcoICRI GAG'CTC
Cuts at: 0 579 897 1134
Size: 579 318 237
Fragments arranged by size:
579 318 237
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 405 580 736 1134
Size: 405 175 156 398
Fragments arranged by size:
405 398 175 156
EcoNI CCTnn'n_nnAGG
Cuts at: 0 751 1042 1134
Size: 751 291 92
Fragments arranged by size:
751 291 92
EcoO109I rG'GnC_Cy
Cuts at: 0 1026 1134
Size: 1026 108
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 411 432 1134
Size: 411 21 702
Fragments arranged by size:
702 411 21
HaeII r_GCGC'y
Cuts at: 0 1089 1134
Size: 1089 45
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 617 649 1134
Size: 617 32 485
Fragments arranged by size:
617 485 32
HincII GTy'rAC
Cuts at: 0 242 963 1134
Size: 242 721 171
Fragments arranged by size:
721 242 171
MscI TGG'CCA
Cuts at: 0 469 712 865 1134
Size: 469 243 153 269
Fragments arranged by size:
469 269 243 153
MslI CAynn'nnrTG
Cuts at: 0 112 670 787 799 1099 1134
Size: 112 558 117 12 300 35
Fragments arranged by size:
558 300 117 112 35 12
MspA1I CmG'CkG
Cuts at: 0 828 953 1134
Size: 828 125 181
Fragments arranged by size:
828 181 125
NspI r_CATG'y
Cuts at: 0 432 649 1134
Size: 432 217 485
Fragments arranged by size:
485 432 217
PflMI CCAn_nnn'nTGG
Cuts at: 0 362 861 1134
Size: 362 499 273
Fragments arranged by size:
499 362 273
PpuMI rG'GwC_Cy
Cuts at: 0 1026 1134
Size: 1026 108
PvuII CAG'CTG
Cuts at: 0 828 1134
Size: 828 306
SacI G_AGCT'C
Cuts at: 0 581 899 1134
Size: 581 318 235
Fragments arranged by size:
581 318 235
SapI GCTCTTCn'nnn_
Cuts at: 0 1024 1134
Size: 1024 110
SfcI C'TryA_G
Cuts at: 0 345 933 1134
Size: 345 588 201
Fragments arranged by size:
588 345 201
SmlI C'TyrA_G
Cuts at: 0 265 1134
Size: 265 869
SphI G_CATG'C
Cuts at: 0 432 1134
Size: 432 702
StuI AGG'CCT
Cuts at: 0 655 1134
Size: 655 479
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 292 1134
Size: 292 842
TatI w'GTAC_w
Cuts at: 0 191 584 1134
Size: 191 393 550
Fragments arranged by size:
550 393 191
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 594 626 1134
Size: 594 32 508
Fragments arranged by size:
594 508 32
ZraI GAC'GTC
Cuts at: 0 927 1134
Size: 927 207
Enzymes that do cut:
AatII AcuI AleI BanII BbsI BbvCI BciVI BclI
BglI Bme1580I BmgBI BmrI BpmI Bpu10I BpuEI BsaI
BsaAI BsaBI BsaHI BsaXI BseRI BsiHKAI Bsp1286I BspMI
BsrDI BsrGI BstAPI BstXI BtgZI BtsI DraI EaeI
EarI EcoICRI Eco57MI EcoNI EcoO109I FalI HaeII Hin4I
HincII MscI MslI MspA1I NspI PflMI PpuMI PvuII
SacI SapI SfcI SmlI SphI StuI TaqII TatI
TstI ZraI
Enzymes that do not cut:
AarI AccI Acc65I AclI AfeI AflII AflIII AgeI
AhdI AlfI AloI AlwNI ApaI ApaLI ApoI AscI
AseI AsiSI AvaI AvrII BaeI BamHI BanI BbeI
BcgI BfrBI BglII BlpI BmtI BplI BsaWI BseYI
BsgI BsiEI BsiWI BsmI BsmBI BspEI BspHI BsrBI
BsrFI BssHII BssSI BstBI BstEII BstYI BstZ17I Bsu36I
BtgI ClaI CspCI DraIII DrdI EagI EciI EcoRI
EcoRV FseI FspI FspAI HindIII HpaI KasI KpnI
MfeI MluI MmeI NaeI NarI NcoI NdeI NgoMIV
NheI NotI NruI NsiI PacI PasI PciI PfoI
PmeI PmlI PpiI PshAI PsiI PspOMI PspXI PsrI
PstI PvuI RsrII SacII SalI SanDI SbfI ScaI
SexAI SfiI SfoI SgfI SgrAI SmaI SnaBI SpeI
SrfI SspI StyI SwaI Tth111I XbaI XcmI XhoI
XmaI XmnI