################################## # NO CUT ENZYMES ( 92 enzymes) # ################################## AatII Acc65I AclI AcsI AflII AgeI Aor51HI ApoI AscI Asp700I Asp718I AspI AvrII BamHI BbrPI BcgI BciVI BclI BfrI BlnI BsaAI BsiEI BsiWI Bsp1407I BspDI BspHI BsrFI BsrGI BssHII BssSI BstBI BstEII BstPI Cfr10I ClaI CpoI DraI DrdI EagI EclXI Eco47III Eco52I EcoO65I EcoRI EcoT22I FbaI FseI HincII HindII HindIII HpaI KpnI MfeI MluI MunI NaeI NdeI NgoMIV NheI NotI NruI NsiI NspV PacI PflFI PinAI PmaCI PmeI PmlI Psp1406I PvuI RcaI RsrII SalI SapI SbfI ScaI SexAI SfiI SfuI SgrAI SnaBI SpeI Sse8387I SspBI SspI SwaI TfiI Tsp509I Tth111I XbaI XmnI ################################### # ONE CUT ENZYMES ( 50 enzymes) # ################################### AccI 1580 GTATAC GT^[AC][GT]AC AcyI 213 GGCGCC G[GA]^CG[CT]C AflIII 1798 ACATGT A^C[GA][CT]GT AhdI 58 GACAAAGTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC Alw44I 205 GTGCAC G^TGCAC ApaI 238 GGGCCC GGGCC^C ApaLI 205 GTGCAC G^TGCAC AseI 907 ATTAAT AT^TAAT AsnI 907 ATTAAT AT^TAAT AspEI 58 GACAAAGTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC AviII 1079 TGCGCA TGC^GCA BalI 1064 TGGCCA TGG^CCA BbeI 213 GGCGCC GGCGC^C BbvCI 145 GCTGAGG CCTCAGC(-5/-2) BsaBI 582 GATGAAAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC BsaHI 213 GGCGCC G[GA]^CG[CT]C BsmBI 2019 CGTCTC CGTCTC(1/5) BsmI 540 GAATGC GAATGC(1/-1) BspLU11I 1798 ACATGT A^CATGT BsrBI 1957 GAGCGG CCGCTC(-3/-3) BsrDI 1082 GCAATG GCAATG(2/0) Bst1107I 1580 GTATAC GTA^TAC BstAPI 1116 GCACCTGCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC BstXI 1742 CCAAAGGCCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG BstZ17I 1580 GTATAC GTA^TAC DraIII 391 CACCCTGTG CAC[ACGT][ACGT][ACGT]^GTG Eam1105I 58 GACAAAGTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC EcoNI 1560 CCTAAACCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG EcoRV 1375 GATATC GAT^ATC FspI 1079 TGCGCA TGC^GCA Hin1I 213 GGCGCC G[GA]^CG[CT]C KasI 213 GGCGCC G^GCGCC KspI 289 CCGCGG CCGC^GG MaeII 179 ACGT A^CGT MamI 582 GATGAAAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC MluNI 1064 TGGCCA TGG^CCA MscI 1064 TGGCCA TGG^CCA MseI 908 TTAA T^TAA MslI 1626 CACCATGGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG NarI 213 GGCGCC GG^CGCC PaeR7I 1941 CTCGAG C^TCGAG PshAI 897 GACCAATGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC PshBI 907 ATTAAT AT^TAAT PstI 352 CTGCAG CTGCA^G SacII 289 CCGCGG CCGC^GG SfoI 213 GGCGCC GGC^GCC SmlI 1941 CTCGAG C^T[CT][GA]AG Tru9I 908 TTAA T^TAA XcmI 720 CCAGTTCCTGAATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG XhoI 1941 CTCGAG C^TCGAG ################################### # TWO CUT ENZYMES ( 23 enzymes) # ################################### AccII 202 CGCG CG^CG 290 CGCG AfaI 362 GTAC GT^AC 444 GTAC AocI 1141 CCTGAGG CC^T[ACGT]AGG 1369 CCTGAGG BanI 213 GGCGCC G^G[CT][GA]CC 1861 GGCACC BfaI 844 CTAG C^TAG 1993 CTAG BglI 37 GCCTTCGGGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC 796 GCCATGGTGGC BglII 1541 AGATCT A^GATCT 1937 AGATCT BstUI 202 CGCG CG^CG 290 CGCG Bsu36I 1141 CCTGAGG CC^T[ACGT]AGG 1369 CCTGAGG EarI 307 CTCTTC CTCTTC(1/4) 1192 CTCTTC Eco81I 1141 CCTGAGG CC^T[ACGT]AGG 1369 CCTGAGG Ksp632I 307 CTCTTC CTCTTC(1/4) 1192 CTCTTC MaeI 844 CTAG C^TAG 1993 CTAG MvnI 202 CGCG CG^CG 290 CGCG NcoI 797 CCATGG C^CATGG 1628 CCATGG PflMI 321 CCAGTGGCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 1067 CCAAGACCTGG RsaI 362 GTAC GT^AC 444 GTAC SfcI 352 CTGCAG C^T[GA][CT]AG 556 CTACAG SphI 1656 GCATGC GCATG^C 1718 GCATGC StuI 1526 AGGCCT AGG^CCT 1746 AGGCCT TaqI 1942 TCGA T^CGA 2009 TCGA TthHB8I 1942 TCGA T^CGA 2009 TCGA Van91I 321 CCAGTGGCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 1067 CCAAGACCTGG ######################################### # 3 OR MORE CUT ENZYMES ( 93 enzymes) # ######################################### AccIII AciI AluI AlwI AlwNI AspHI AvaI AvaII BanII BbsI BbvI BcnI BlpI BmyI BpmI Bpu1102I BpuAI BsaI BsaJI BsaWI BseAI BseRI BsgI BsiHKAI BsiYI BslI BsmAI BsmFI BsoBI Bsp1286I BspEI BspMI BsrI BssKI BstF5I BstNI BstYI Cac8I CelII CfoI Cfr13I DdeI DpnII DraII DsaI EaeI EcoO109I EcoRII EcoT14I FauI Fnu4HI FokI HaeII HaeIII HapII HgaI HhaI HinP1I HinfI HpaII HphI ItaI MaeIII MboI MboII MflI MnlI MroI MspA1I MspI MvaI MwoI NciI NdeII NlaIII NlaIV NspI PleI PpuMI PvuII SacI Sau3AI Sau96I ScrFI SfaNI SmaI StyI TseI Tsp45I TspRI XhoII XmaCI XmaI