(Circular) (Six-base) MAPSORT of: pFN21AE1570.seq Check: 3378 from: 1 to: 6733 pFN21AE1570 6733 bp With 182 enzymes: SgfI * August 16, 2010 14:48 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 3132 3268 3656 3132 Size: 136 388 6209 Fragments arranged by size: 6209 388 136 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 4543 278 Size: 53 83 186 936 3007 2468 Fragments arranged by size: 3007 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3586 4076 1937 Size: 1649 490 4594 Fragments arranged by size: 4594 1649 490 Acc65I G'GTAC_C Cuts at: 4054 4054 Size: 6733 AclI AA'CG_TT Cuts at: 1563 4860 5233 1563 Size: 3297 373 3063 Fragments arranged by size: 3297 3063 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2414 4796 6180 6448 1632 Size: 782 2382 1384 268 1917 Fragments arranged by size: 2382 1917 1384 782 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 6536 AflIII A'CryG_T Cuts at: 1203 3803 5632 6609 1203 Size: 2600 1829 977 1327 Fragments arranged by size: 2600 1829 1327 977 AgeI A'CCGG_T Cuts at: 5564 5564 Size: 6733 AhdI GACnn_n'nnGTC Cuts at: 2068 5462 2068 Size: 3394 3339 AleI CACnn'nnGTG Cuts at: 1166 3635 1166 Size: 2469 4264 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 2724 3746 1184 Size: 1540 1022 4171 Fragments arranged by size: 4171 1540 1022 AloI GAACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2249 2281 4010 4042 2249 Size: 32 1729 32 4940 Fragments arranged by size: 4940 1729 32 32 AlwNI CAG_nnn'CTG Cuts at: 2332 2678 2731 4476 6048 2332 Size: 346 53 1745 1572 3017 Fragments arranged by size: 3017 1745 1572 346 53 ApaI G_GGCC'C Cuts at: 2247 2247 Size: 6733 ApaLI G'TGCA_C Cuts at: 2210 4790 5946 2210 Size: 2580 1156 2997 Fragments arranged by size: 2997 2580 1156 ApoI r'AATT_y Cuts at: 1493 4042 4268 4420 6529 1493 Size: 2549 226 152 2109 1697 Fragments arranged by size: 2549 2109 1697 226 152 AseI AT'TA_AT Cuts at: 160 1032 2913 5287 160 Size: 872 1881 2374 1606 Fragments arranged by size: 2374 1881 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6733 AvaI C'yCGr_G Cuts at: 1870 1943 2091 3252 3390 3606 3946 4058 1870 Size: 73 148 1161 138 216 340 112 4545 Fragments arranged by size: 4545 1161 340 216 148 138 112 73 BamHI G'GATC_C Cuts at: 4063 4430 4063 Size: 367 6366 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2218 3866 4054 5509 618 Size: 325 217 627 431 1648 188 1455 1842 Fragments arranged by size: 1842 1648 1455 627 431 325 217 188 BanII G_rGCy'C Cuts at: 1391 2105 2247 2520 2622 2861 2966 3528 3581 Size: 714 142 273 102 239 105 562 53 Cuts at: 3581 4052 1391 Size: 471 4072 Fragments arranged by size: 4072 714 562 471 273 239 142 105 102 53 BbeI G_GCGC'C Cuts at: 2222 2222 Size: 6733 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2514 3145 3408 928 Size: 971 615 631 263 4253 Fragments arranged by size: 4253 971 631 615 263 BbvCI CC'TCA_GC Cuts at: 2151 2151 Size: 6733 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 4924 4958 1903 Size: 34 2987 34 3678 Fragments arranged by size: 3678 2987 34 34 BciVI GTATCCnnnnn_n' Cuts at: 4625 5835 4625 Size: 1210 5523 BclI T'GATC_A Cuts at: 1548 1548 Size: 6733 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2048 2807 5344 Size: 107 122 71 631 605 376 759 2537 Cuts at: 5344 136 Size: 1525 Fragments arranged by size: 2537 1525 759 631 605 376 122 107 71 BglII A'GATC_T Cuts at: 3546 3942 6728 3546 Size: 396 2786 3551 Fragments arranged by size: 3551 2786 396 BlpI GC'TnA_GC Cuts at: 2679 2844 3002 3652 3833 4150 2679 Size: 165 158 650 181 317 5262 Fragments arranged by size: 5262 650 317 181 165 158 Bme1580I G_kGCm'C Cuts at: 1790 2214 2247 3869 4794 5950 1790 Size: 424 33 1622 925 1156 2573 Fragments arranged by size: 2573 1622 1156 925 424 33 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 6733 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2219 2719 4464 Size: 416 53 83 399 24 802 500 1745 Cuts at: 4464 5422 442 Size: 958 1753 Fragments arranged by size: 1753 1745 958 802 500 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6733 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2875 3568 2875 Size: 693 6040 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2354 2390 2644 3512 3620 3867 5393 1239 Size: 1115 36 254 868 108 247 1526 2579 Fragments arranged by size: 2579 1526 1115 868 254 247 108 36 Bpu10I CC'TnA_GC Cuts at: 2151 3580 2151 Size: 1429 5304 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 4858 5723 6021 4858 Size: 865 298 5570 Fragments arranged by size: 5570 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2744 3596 3843 5396 882 Size: 617 1245 852 247 1553 2219 Fragments arranged by size: 2219 1553 1245 852 617 247 BsaAI yAC'GTr Cuts at: 493 1204 4524 493 Size: 711 3320 2702 Fragments arranged by size: 3320 2702 711 BsaBI GATnn'nnATC Cuts at: 2591 6727 2591 Size: 4136 2597 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2219 4540 4922 5557 Size: 53 83 186 936 686 2321 382 635 Cuts at: 5557 275 Size: 1451 Fragments arranged by size: 2321 1451 936 686 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 2033 2661 3384 3874 5166 5564 5838 5985 6396 Size: 628 723 490 1292 398 274 147 411 Cuts at: 6396 2033 Size: 2370 Fragments arranged by size: 2370 1292 723 628 490 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 4010 4040 1178 Size: 30 165 30 2607 30 3871 Fragments arranged by size: 3871 2607 165 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2168 2300 2438 2641 3408 3583 3908 1186 Size: 982 132 138 203 767 175 325 4011 Fragments arranged by size: 4011 982 767 325 203 175 138 132 BseYI C'CCAG_C Cuts at: 2239 2671 3719 5936 6432 2239 Size: 432 1048 2217 496 2540 Fragments arranged by size: 2540 2217 1048 496 432 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 3113 3218 3471 3641 3696 1163 Size: 573 1377 105 253 170 55 4200 Fragments arranged by size: 4200 1377 573 253 170 105 55 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 4183 4944 5972 1540 Size: 275 186 2182 761 1028 2301 Fragments arranged by size: 2301 2182 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2214 2406 2520 2685 2697 3528 3581 3885 4794 Size: 192 114 165 12 831 53 304 909 Cuts at: 4794 4879 5950 2214 Size: 85 1071 2997 Fragments arranged by size: 2997 1071 909 831 304 192 165 114 85 53 12 BsmI GAATG_Cn' Cuts at: 2551 4244 4337 2551 Size: 1693 93 4947 Fragments arranged by size: 4947 1693 93 BsmBI CGTCTCn'nnnn_ Cuts at: 4030 4030 Size: 6733 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2105 2214 2247 2406 2520 2622 2685 Size: 399 315 109 33 159 114 102 63 Cuts at: 2685 2697 2861 2966 3528 3581 3869 3885 4052 Size: 12 164 105 562 53 288 16 167 Cuts at: 4052 4794 4879 5950 1391 Size: 742 85 1071 2174 Fragments arranged by size: 2174 1071 742 562 399 315 288 167 164 159 114 109 105 102 85 63 53 33 16 12 BspEI T'CCGG_A Cuts at: 2661 3384 3874 6396 2661 Size: 723 490 2522 2998 Fragments arranged by size: 2998 2522 723 490 BspHI T'CATG_A Cuts at: 4622 6352 4622 Size: 1730 5003 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 3132 3268 3656 3720 4088 844 Size: 2288 136 388 64 368 3489 Fragments arranged by size: 3489 2288 388 368 136 64 BsrBI CCG'CTC Cuts at: 1394 1823 3964 4620 1394 Size: 429 2141 656 3507 Fragments arranged by size: 3507 2141 656 429 BsrDI GCAATG_nn' Cuts at: 65 1248 3094 5228 5402 65 Size: 1183 1846 2134 174 1396 Fragments arranged by size: 2134 1846 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 5377 5564 1700 Size: 110 3567 187 2869 Fragments arranged by size: 3567 2869 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 6733 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6733 BssSI C'ACGA_G Cuts at: 4793 5805 4793 Size: 1012 5721 BstAPI GCAn_nnn'nTGC Cuts at: 794 3127 794 Size: 2333 4400 BstBI TT'CG_AA Cuts at: 5537 5537 Size: 6733 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 6733 BstXI CCAn_nnnn'nTGG Cuts at: 3754 3754 Size: 6733 BstYI r'GATC_y Cuts at: 1972 2649 3546 3942 4063 4430 4815 4832 6284 Size: 677 897 396 121 367 385 17 1452 Cuts at: 6284 6370 6382 6728 1972 Size: 86 12 346 1977 Fragments arranged by size: 1977 1452 897 677 396 385 367 346 121 86 17 12 BstZ17I GTA'TAC Cuts at: 3587 3587 Size: 6733 Bsu36I CC'TnA_GG Cuts at: 3147 3375 3147 Size: 228 6505 BtgI C'CryG_G Cuts at: 513 1065 2004 2294 2802 3633 513 Size: 552 939 290 508 831 3613 Fragments arranged by size: 3613 939 831 552 508 290 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 3898 487 Size: 683 56 2672 3322 Fragments arranged by size: 3322 2672 683 56 BtsI GCAGTG_nn' Cuts at: 2108 2139 2482 3184 3459 4249 5043 5063 2108 Size: 31 343 702 275 790 794 20 3778 Fragments arranged by size: 3778 794 790 702 343 275 31 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6733 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 6698 DraI TTT'AAA Cuts at: 4037 4037 Size: 6733 DraIII CAC_nnn'GTG Cuts at: 2401 2401 Size: 6733 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 5740 809 Size: 507 4424 1802 Fragments arranged by size: 4424 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2007 2302 3069 Size: 54 1425 50 275 18 177 295 767 Cuts at: 3069 4180 5069 6698 8 Size: 1111 889 1629 43 Fragments arranged by size: 1629 1425 1111 889 767 295 275 177 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 4180 1537 Size: 275 2368 4090 Fragments arranged by size: 4090 2368 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2318 3203 4663 1353 Size: 965 885 1460 3423 Fragments arranged by size: 3423 1460 965 885 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2747 3922 5320 5694 5840 387 Size: 1064 138 1158 1175 1398 374 146 1280 Fragments arranged by size: 1398 1280 1175 1158 1064 374 146 138 EcoICRI GAG'CTC Cuts at: 2518 3526 3579 2518 Size: 1008 53 5672 Fragments arranged by size: 5672 1008 53 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2354 2390 2414 2644 3512 3620 3867 Size: 393 722 36 24 230 868 108 247 Cuts at: 3867 4796 5393 6180 6448 1239 Size: 929 597 787 268 1524 Fragments arranged by size: 1524 929 868 787 722 597 393 268 247 230 108 36 24 EcoNI CCTnn'n_nnAGG Cuts at: 3569 3569 Size: 6733 EcoO109I rG'GnC_Cy Cuts at: 2088 2243 2244 2346 2667 2822 3112 3858 3898 Size: 155 1 102 321 155 290 746 40 Cuts at: 3898 3973 2088 Size: 75 4848 Fragments arranged by size: 4848 746 321 290 155 155 102 75 40 1 EcoRI G'AATT_C Cuts at: 4042 4420 4042 Size: 378 6355 EcoRV GAT'ATC Cuts at: 3382 3382 Size: 6733 FspI TGC'GCA Cuts at: 3086 5239 3086 Size: 2153 4580 HaeII r_GCGC'y Cuts at: 1678 2151 2222 2419 5880 1678 Size: 473 71 197 3461 2531 Fragments arranged by size: 3461 2531 473 197 71 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2054 2086 5374 5406 5448 Size: 32 135 32 482 32 3288 32 42 Cuts at: 5448 5480 1373 Size: 32 2626 Fragments arranged by size: 3288 2626 482 135 42 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 4077 4323 669 Size: 988 281 2139 246 3079 Fragments arranged by size: 3079 2139 988 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 6733 HpaI GTT'AAC Cuts at: 4323 4323 Size: 6733 KasI G'GCGC_C Cuts at: 2218 2218 Size: 6733 KpnI G_GTAC'C Cuts at: 4058 4058 Size: 6733 MfeI C'AATT_G Cuts at: 4332 4332 Size: 6733 MluI A'CGCG_T Cuts at: 6609 6609 Size: 6733 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 5847 6031 1305 Size: 4542 184 2007 Fragments arranged by size: 4542 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 3071 10 Size: 54 1425 343 1239 3672 Fragments arranged by size: 3672 1425 1239 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 3635 4691 5050 5209 518 Size: 648 2469 1056 359 159 2042 Fragments arranged by size: 2469 2042 1056 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2274 2284 2296 2678 2763 2980 3178 3349 Size: 668 10 12 382 85 217 198 171 Cuts at: 3349 3730 4149 4826 5974 6219 1606 Size: 381 419 677 1148 245 2120 Fragments arranged by size: 2120 1148 677 668 419 382 381 245 217 198 171 85 12 10 NarI GG'CG_CC Cuts at: 2219 2219 Size: 6733 NcoI C'CATG_G Cuts at: 513 1065 2004 2802 3633 513 Size: 552 939 798 831 3613 Fragments arranged by size: 3613 939 831 798 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 6733 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6733 NotI GC'GGCC_GC Cuts at: 4180 4180 Size: 6733 NspI r_CATG'y Cuts at: 1132 1229 3665 3727 3765 3807 4091 5636 1132 Size: 97 2436 62 38 42 284 1545 2229 Fragments arranged by size: 2436 2229 1545 284 97 62 42 38 PasI CC'CwG_GG Cuts at: 1348 3862 1348 Size: 2514 4219 PciI A'CATG_T Cuts at: 3803 5632 3803 Size: 1829 4904 PflMI CCAn_nnn'nTGG Cuts at: 1108 2332 3078 1108 Size: 1224 746 4763 Fragments arranged by size: 4763 1224 746 PfoI T'CCnGG_A Cuts at: 2175 3957 2175 Size: 1782 4951 PmeI GTTT'AAAC Cuts at: 4037 4037 Size: 6733 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4010 4042 4802 4834 6315 6347 4010 Size: 32 760 32 1481 32 4396 Fragments arranged by size: 4396 1481 760 32 32 32 PpuMI rG'GwC_Cy Cuts at: 2088 2346 2667 2822 2088 Size: 258 321 155 5999 Fragments arranged by size: 5999 321 258 155 PshAI GACnn'nnGTC Cuts at: 1613 2906 1613 Size: 1293 5440 PsiI TTA'TAA Cuts at: 4303 4303 Size: 6733 PspOMI G'GGCC_C Cuts at: 2243 2243 Size: 6733 PstI C_TGCA'G Cuts at: 830 2361 4085 830 Size: 1531 1724 3478 Fragments arranged by size: 3478 1724 1531 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6733 PvuII CAG'CTG Cuts at: 2284 2678 3178 3349 3730 2284 Size: 394 500 171 381 5287 Fragments arranged by size: 5287 500 394 381 171 SacI G_AGCT'C Cuts at: 2520 3528 3581 2520 Size: 1008 53 5672 Fragments arranged by size: 5672 1008 53 SacII CC_GC'GG Cuts at: 2297 2297 Size: 6733 SalI G'TCGA_C Cuts at: 1936 4075 1936 Size: 2139 4594 SanDI GG'GwC_CC Cuts at: 2088 2346 2088 Size: 258 6475 SbfI CC_TGCA'GG Cuts at: 4085 4085 Size: 6733 ScaI AGT'ACT Cuts at: 4981 6693 4981 Size: 1712 5021 SfcI C'TryA_G Cuts at: 826 1045 2357 2561 4081 5216 5897 6088 826 Size: 219 1312 204 1520 1135 681 191 1471 Fragments arranged by size: 1520 1471 1312 1135 681 219 204 191 SfoI GGC'GCC Cuts at: 2220 2220 Size: 6733 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6733 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6733 SmaI CCC'GGG Cuts at: 1872 2093 3254 3608 4060 1872 Size: 221 1161 354 452 4545 Fragments arranged by size: 4545 1161 452 354 221 SmlI C'TyrA_G Cuts at: 820 1017 1943 3946 4837 5738 6000 820 Size: 197 926 2003 891 901 262 1553 Fragments arranged by size: 2003 1553 926 901 891 262 197 SnaBI TAC'GTA Cuts at: 493 4524 493 Size: 4031 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 6733 SphI G_CATG'C Cuts at: 1132 3665 3727 4091 1132 Size: 2533 62 364 3774 Fragments arranged by size: 3774 2533 364 62 SspI AAT'ATT Cuts at: 5 52 4657 5 Size: 47 4605 2081 Fragments arranged by size: 4605 2081 47 StuI AGG'CCT Cuts at: 1517 3533 3753 1517 Size: 2016 220 4497 Fragments arranged by size: 4497 2016 220 StyI C'CwwG_G Cuts at: 513 1065 2004 2373 2802 3555 3633 4172 513 Size: 552 939 369 429 753 78 539 3074 Fragments arranged by size: 3074 939 753 552 539 429 369 78 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 3070 3733 3754 4760 4777 4930 5115 Size: 1255 1490 663 21 1006 17 153 185 Cuts at: 5115 5560 325 Size: 445 1498 Fragments arranged by size: 1498 1490 1255 1006 663 445 185 153 21 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 4979 6691 96 Size: 274 80 33 51 1443 3002 1712 138 Fragments arranged by size: 3002 1712 1443 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 6693 6725 6693 Size: 32 6701 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 6733 XbaI T'CTAG_A Cuts at: 4069 4069 Size: 6733 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2732 2732 Size: 6733 XhoI C'TCGA_G Cuts at: 1943 3946 1943 Size: 2003 4730 XmaI C'CCGG_G Cuts at: 1870 2091 3252 3606 4058 1870 Size: 221 1161 354 452 4545 Fragments arranged by size: 4545 1161 452 354 221 XmnI GAAnn'nnTTC Cuts at: 4862 4862 Size: 6733 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 4541 276 Size: 53 83 186 936 3007 2468 Fragments arranged by size: 3007 2468 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AloI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BstZ17I Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI EcoRV FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NarI NcoI NdeI NheI NotI NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PspOMI PstI PvuI PvuII SacI SacII SalI SanDI SbfI ScaI SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AfeI AscI AvrII BaeI BfrBI BsiWI FalI FseI FspAI NaeI NgoMIV NruI NsiI PacI PmlI PspXI PsrI RsrII SapI SexAI SfiI SrfI SwaI