(Linear) (Six-base) MAPSORT of: KIEE1570.seq Check: 7647 from: 1 to: 2028 KIEE1570.orf With 182 enzymes: SgfI * July 15, 2010 18:42 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 0 1127 1263 1651 2028 Size: 1127 136 388 377 Fragments arranged by size: 1127 388 377 136 AccI GT'mk_AC Cuts at: 0 1581 2028 Size: 1581 447 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 0 409 2028 Size: 409 1619 AflIII A'CryG_T Cuts at: 0 1798 2028 Size: 1798 230 AhdI GACnn_n'nnGTC Cuts at: 0 63 2028 Size: 63 1965 AleI CACnn'nnGTG Cuts at: 0 1630 2028 Size: 1630 398 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 0 719 1741 2028 Size: 719 1022 287 Fragments arranged by size: 1022 719 287 AloI GAACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 0 244 276 2005 2028 Size: 244 32 1729 23 Fragments arranged by size: 1729 244 32 23 AlwNI CAG_nnn'CTG Cuts at: 0 327 673 726 2028 Size: 327 346 53 1302 Fragments arranged by size: 1302 346 327 53 ApaI G_GGCC'C Cuts at: 0 242 2028 Size: 242 1786 ApaLI G'TGCA_C Cuts at: 0 205 2028 Size: 205 1823 AseI AT'TA_AT Cuts at: 0 908 2028 Size: 908 1120 AvaI C'yCGr_G Cuts at: 0 86 1247 1385 1601 1941 2028 Size: 86 1161 138 216 340 87 Fragments arranged by size: 1161 340 216 138 87 86 BanI G'GyrC_C Cuts at: 0 213 1861 2028 Size: 213 1648 167 Fragments arranged by size: 1648 213 167 BanII G_rGCy'C Cuts at: 0 100 242 515 617 856 961 1523 1576 Size: 100 142 273 102 239 105 562 53 Cuts at: 1576 2028 Size: 452 Fragments arranged by size: 562 452 273 239 142 105 102 100 53 BbeI G_GCGC'C Cuts at: 0 217 2028 Size: 217 1811 BbsI GAAGACnn'nnnn_ Cuts at: 0 509 1140 1403 2028 Size: 509 631 263 625 Fragments arranged by size: 631 625 509 263 BbvCI CC'TCA_GC Cuts at: 0 146 2028 Size: 146 1882 BglI GCCn_nnn'nGGC Cuts at: 0 43 802 2028 Size: 43 759 1226 Fragments arranged by size: 1226 759 43 BglII A'GATC_T Cuts at: 0 1541 1937 2028 Size: 1541 396 91 Fragments arranged by size: 1541 396 91 BlpI GC'TnA_GC Cuts at: 0 674 839 997 1647 1828 2028 Size: 674 165 158 650 181 200 Fragments arranged by size: 674 650 200 181 165 158 Bme1580I G_kGCm'C Cuts at: 0 209 242 1864 2028 Size: 209 33 1622 164 Fragments arranged by size: 1622 209 164 33 BmrI ACTGGGnnnn_n' Cuts at: 0 214 714 2028 Size: 214 500 1314 Fragments arranged by size: 1314 500 214 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 0 870 1563 2028 Size: 870 693 465 Fragments arranged by size: 870 693 465 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 349 385 639 1507 1615 1862 2028 Size: 349 36 254 868 108 247 166 Fragments arranged by size: 868 349 254 247 166 108 36 Bpu10I CC'TnA_GC Cuts at: 0 146 1575 2028 Size: 146 1429 453 Fragments arranged by size: 1429 453 146 BsaI GGTCTCn'nnnn_ Cuts at: 0 739 1591 1838 2028 Size: 739 852 247 190 Fragments arranged by size: 852 739 247 190 BsaBI GATnn'nnATC Cuts at: 0 586 2028 Size: 586 1442 BsaHI Gr'CG_yC Cuts at: 0 214 2028 Size: 214 1814 BsaWI w'CCGG_w Cuts at: 0 28 656 1379 1869 2028 Size: 28 628 723 490 159 Fragments arranged by size: 723 628 490 159 28 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 0 2005 2028 Size: 2005 23 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 0 163 295 433 636 1403 1578 1903 2028 Size: 163 132 138 203 767 175 325 125 Fragments arranged by size: 767 325 203 175 163 138 132 125 BseYI C'CCAG_C Cuts at: 0 234 666 1714 2028 Size: 234 432 1048 314 Fragments arranged by size: 1048 432 314 234 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 0 1108 1213 1466 1636 1691 2028 Size: 1108 105 253 170 55 337 Fragments arranged by size: 1108 337 253 170 105 55 BsiHKAI G_wGCw'C Cuts at: 0 209 401 515 680 692 1523 1576 1880 Size: 209 192 114 165 12 831 53 304 Cuts at: 1880 2028 Size: 148 Fragments arranged by size: 831 304 209 192 165 148 114 53 12 BsmI GAATG_Cn' Cuts at: 0 546 2028 Size: 546 1482 BsmBI CGTCTCn'nnnn_ Cuts at: 0 2025 2028 Size: 2025 3 Bsp1286I G_dGCh'C Cuts at: 0 100 209 242 401 515 617 680 692 Size: 100 109 33 159 114 102 63 12 Cuts at: 692 856 961 1523 1576 1864 1880 2028 Size: 164 105 562 53 288 16 148 Fragments arranged by size: 562 288 164 159 148 114 109 105 102 100 63 53 33 16 12 BspEI T'CCGG_A Cuts at: 0 656 1379 1869 2028 Size: 656 723 490 159 Fragments arranged by size: 723 656 490 159 BspMI ACCTGCnnnn'nnnn_ Cuts at: 0 1127 1263 1651 1715 2028 Size: 1127 136 388 64 313 Fragments arranged by size: 1127 388 313 136 64 BsrBI CCG'CTC Cuts at: 0 1959 2028 Size: 1959 69 BsrDI GCAATG_nn' Cuts at: 0 1089 2028 Size: 1089 939 BstAPI GCAn_nnn'nTGC Cuts at: 0 1122 2028 Size: 1122 906 BstXI CCAn_nnnn'nTGG Cuts at: 0 1749 2028 Size: 1749 279 BstYI r'GATC_y Cuts at: 0 644 1541 1937 2028 Size: 644 897 396 91 Fragments arranged by size: 897 644 396 91 BstZ17I GTA'TAC Cuts at: 0 1582 2028 Size: 1582 446 Bsu36I CC'TnA_GG Cuts at: 0 1142 1370 2028 Size: 1142 228 658 Fragments arranged by size: 1142 658 228 BtgI C'CryG_G Cuts at: 0 289 797 1628 2028 Size: 289 508 831 400 Fragments arranged by size: 831 508 400 289 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 0 1893 2028 Size: 1893 135 BtsI GCAGTG_nn' Cuts at: 0 103 134 477 1179 1454 2028 Size: 103 31 343 702 275 574 Fragments arranged by size: 702 574 343 275 103 31 DraIII CAC_nnn'GTG Cuts at: 0 396 2028 Size: 396 1632 EaeI y'GGCC_r Cuts at: 0 2 297 1064 2028 Size: 2 295 767 964 Fragments arranged by size: 964 767 295 2 EarI CTCTTCn'nnn_ Cuts at: 0 313 1198 2028 Size: 313 885 830 Fragments arranged by size: 885 830 313 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 0 742 1917 2028 Size: 742 1175 111 Fragments arranged by size: 1175 742 111 EcoICRI GAG'CTC Cuts at: 0 513 1521 1574 2028 Size: 513 1008 53 454 Fragments arranged by size: 1008 513 454 53 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 0 349 385 409 639 1507 1615 1862 2028 Size: 349 36 24 230 868 108 247 166 Fragments arranged by size: 868 349 247 230 166 108 36 24 EcoNI CCTnn'n_nnAGG Cuts at: 0 1564 2028 Size: 1564 464 EcoO109I rG'GnC_Cy Cuts at: 0 83 238 239 341 662 817 1107 1853 Size: 83 155 1 102 321 155 290 746 Cuts at: 1853 1893 1968 2028 Size: 40 75 60 Fragments arranged by size: 746 321 290 155 155 102 83 75 60 40 1 EcoRV GAT'ATC Cuts at: 0 1377 2028 Size: 1377 651 FspI TGC'GCA Cuts at: 0 1081 2028 Size: 1081 947 HaeII r_GCGC'y Cuts at: 0 146 217 414 2028 Size: 146 71 197 1614 Fragments arranged by size: 1614 197 146 71 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 0 49 81 2028 Size: 49 32 1947 Fragments arranged by size: 1947 49 32 KasI G'GCGC_C Cuts at: 0 213 2028 Size: 213 1815 MscI TGG'CCA Cuts at: 0 1066 2028 Size: 1066 962 MslI CAynn'nnrTG Cuts at: 0 1630 2028 Size: 1630 398 MspA1I CmG'CkG Cuts at: 0 269 279 291 673 758 975 1173 1344 Size: 269 10 12 382 85 217 198 171 Cuts at: 1344 1725 2028 Size: 381 303 Fragments arranged by size: 382 381 303 269 217 198 171 85 12 10 NarI GG'CG_CC Cuts at: 0 214 2028 Size: 214 1814 NcoI C'CATG_G Cuts at: 0 797 1628 2028 Size: 797 831 400 Fragments arranged by size: 831 797 400 NspI r_CATG'y Cuts at: 0 1660 1722 1760 1802 2028 Size: 1660 62 38 42 226 Fragments arranged by size: 1660 226 62 42 38 PasI CC'CwG_GG Cuts at: 0 1857 2028 Size: 1857 171 PciI A'CATG_T Cuts at: 0 1798 2028 Size: 1798 230 PflMI CCAn_nnn'nTGG Cuts at: 0 327 1073 2028 Size: 327 746 955 Fragments arranged by size: 955 746 327 PfoI T'CCnGG_A Cuts at: 0 170 1952 2028 Size: 170 1782 76 Fragments arranged by size: 1782 170 76 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 0 2005 2028 Size: 2005 23 PpuMI rG'GwC_Cy Cuts at: 0 83 341 662 817 2028 Size: 83 258 321 155 1211 Fragments arranged by size: 1211 321 258 155 83 PshAI GACnn'nnGTC Cuts at: 0 901 2028 Size: 901 1127 PspOMI G'GGCC_C Cuts at: 0 238 2028 Size: 238 1790 PstI C_TGCA'G Cuts at: 0 356 2028 Size: 356 1672 PvuII CAG'CTG Cuts at: 0 279 673 1173 1344 1725 2028 Size: 279 394 500 171 381 303 Fragments arranged by size: 500 394 381 303 279 171 SacI G_AGCT'C Cuts at: 0 515 1523 1576 2028 Size: 515 1008 53 452 Fragments arranged by size: 1008 515 452 53 SacII CC_GC'GG Cuts at: 0 292 2028 Size: 292 1736 SanDI GG'GwC_CC Cuts at: 0 83 341 2028 Size: 83 258 1687 Fragments arranged by size: 1687 258 83 SfcI C'TryA_G Cuts at: 0 352 556 2028 Size: 352 204 1472 Fragments arranged by size: 1472 352 204 SfoI GGC'GCC Cuts at: 0 215 2028 Size: 215 1813 SmaI CCC'GGG Cuts at: 0 88 1249 1603 2028 Size: 88 1161 354 425 Fragments arranged by size: 1161 425 354 88 SmlI C'TyrA_G Cuts at: 0 1941 2028 Size: 1941 87 SphI G_CATG'C Cuts at: 0 1660 1722 2028 Size: 1660 62 306 Fragments arranged by size: 1660 306 62 StuI AGG'CCT Cuts at: 0 1528 1748 2028 Size: 1528 220 280 Fragments arranged by size: 1528 280 220 StyI C'CwwG_G Cuts at: 0 368 797 1550 1628 2028 Size: 368 429 753 78 400 Fragments arranged by size: 753 429 400 368 78 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 0 1065 1728 1749 2028 Size: 1065 663 21 279 Fragments arranged by size: 1065 663 279 21 XcmI CCAnnnn_n'nnnnTGG Cuts at: 0 727 2028 Size: 727 1301 XhoI C'TCGA_G Cuts at: 0 1941 2028 Size: 1941 87 XmaI C'CCGG_G Cuts at: 0 86 1247 1601 2028 Size: 86 1161 354 427 Fragments arranged by size: 1161 427 354 86 Enzymes that do cut: AarI AccI AcuI AflIII AhdI AleI AlfI AloI AlwNI ApaI ApaLI AseI AvaI BanI BanII BbeI BbsI BbvCI BglI BglII BlpI Bme1580I BmrI BplI BpmI Bpu10I BsaI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspMI BsrBI BsrDI BstAPI BstXI BstYI BstZ17I Bsu36I BtgI BtgZI BtsI DraIII EaeI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRV FspI HaeII Hin4I KasI MscI MslI MspA1I NarI NcoI NspI PasI PciI PflMI PfoI PpiI PpuMI PshAI PspOMI PstI PvuII SacI SacII SanDI SfcI SfoI SmaI SmlI SphI StuI StyI TaqII XcmI XhoI XmaI Enzymes that do not cut: AatII Acc65I AclI AfeI AflII AgeI ApoI AscI AsiSI AvrII BaeI BamHI BcgI BciVI BclI BfrBI BmgBI BmtI BpuEI BsaAI BsiEI BsiWI BspHI BsrFI BsrGI BssHII BssSI BstBI BstEII ClaI CspCI DraI DrdI EagI EcoRI FalI FseI FspAI HincII HindIII HpaI KpnI MfeI MluI MmeI NaeI NdeI NgoMIV NheI NotI NruI NsiI PacI PmeI PmlI PsiI PspXI PsrI PvuI RsrII SalI SapI SbfI ScaI SexAI SfiI SgfI SgrAI SnaBI SpeI SrfI SspI SwaI TatI TstI Tth111I XbaI XmnI ZraI