################################## # NO CUT ENZYMES ( 129 enzymes) # ################################## AatII Acc65I AclI AflII AgeI AhdI Alw44I AlwI AlwNI AocI Aor51HI ApaI ApaLI AscI AseI AsnI Asp700I Asp718I AspEI AspHI AspI AviII AvrII BalI BamHI BbrPI BbsI BcgI BciVI BclI BfrI BglI BlnI BlpI Bpu1102I BpuAI BsaAI BsaBI BsgI BsiEI BsiHKAI BsiWI BsmBI BsmI BspDI BsrDI BsrFI BssHII Bst1107I BstAPI BstBI BstEII BstPI BstZ17I Bsu36I CelII Cfr10I ClaI CpoI DraIII DrdI EagI Eam1105I EclXI Eco47III Eco52I Eco81I EcoO65I EcoRI EcoRV EcoT14I FbaI FseI FspI HincII HindII HindIII HpaI HphI KpnI KspI MamI MfeI MluI MluNI MscI MunI NaeI NcoI NdeI NgoMIV NheI NotI NspV PacI PaeR7I PflFI PflMI PinAI PmaCI PmeI PmlI PshAI PshBI Psp1406I PvuI RsrII SacI SacII SalI SapI SbfI ScaI SexAI SfiI SfuI SgrAI SnaBI SpeI Sse8387I StuI StyI SwaI Tsp45I Tth111I Van91I XbaI XhoI XmnI ################################### # ONE CUT ENZYMES ( 44 enzymes) # ################################### AccI 475 GTCTAC GT^[AC][GT]AC AccIII 100 TCCGGA T^CCGGA AcyI 72 GGCGCC G[GA]^CG[CT]C AflIII 740 ACATGT A^C[GA][CT]GT AvaI 57 CCCGGG C^[CT]CG[GA]G BanI 72 GGCGCC G^G[CT][GA]CC BanII 124 GAGCCC G[GA]GC[CT]^C BbeI 72 GGCGCC GGCGC^C BbvCI 540 CCTCAGC CCTCAGC(-5/-2) BfaI 928 CTAG C^TAG BmyI 124 GAGCCC G[AGT]GC[ACT]^C BsaHI 72 GGCGCC G[GA]^CG[CT]C BsaI 865 GAGACC GGTCTC(1/5) BsaWI 100 TCCGGA [AT]^CCGG[AT] BseAI 100 TCCGGA T^CCGGA BseRI 169 GAGGAG GAGGAG(10/8) BsmFI 1225 GTCCC GGGAC(10/14) BsoBI 57 CCCGGG C^[CT]CG[GA]G Bsp1286I 124 GAGCCC G[AGT]GC[ACT]^C BspEI 100 TCCGGA T^CCGGA BspHI 206 TCATGA T^CATGA BspLU11I 740 ACATGT A^CATGT BsrBI 887 GAGCGG CCGCTC(-3/-3) BssSI 531 CACGAG CACGAG(-5/-1) BstXI 223 CCAGAATATTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG DraI 454 TTTAAA TTT^AAA EaeI 1156 CGGCCA [CT]^GGCC[GA] EcoNI 768 CCTGGCTCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG HaeII 72 GGCGCC [GA]GCGC^[CT] Hin1I 72 GGCGCC G[GA]^CG[CT]C KasI 72 GGCGCC G^GCGCC MaeI 928 CTAG C^TAG MroI 100 TCCGGA T^CCGGA NarI 72 GGCGCC GG^CGCC PpuMI 60 GGGTCCT [GA]G^G[AT]CC[CT] RcaI 206 TCATGA T^CATGA SfoI 72 GGCGCC GGC^GCC SmaI 57 CCCGGG CCC^GGG SphI 392 GCATGC GCATG^C SspI 227 AATATT AAT^ATT TfiI 824 GATTC G^A[AT]TC XcmI 828 CCATGACATCTGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG XmaCI 57 CCCGGG C^CCGGG XmaI 57 CCCGGG C^CCGGG ################################### # TWO CUT ENZYMES ( 38 enzymes) # ################################### AcsI 322 AAATTT [GA]^AATT[CT] 997 GAATTT ApoI 322 AAATTT [GA]^AATT[CT] 997 GAATTT BcnI 57 CCCGG CC^[GC]GG 58 CCGGG BglII 162 AGATCT A^GATCT 350 AGATCT BpmI 166 CTGGAG CTGGAG(16/14) 803 CTGGAG BsmAI 313 GAGAC GTCTC(1/5) 865 GAGAC Bsp1407I 239 TGTACA T^GTACA 1084 TGTACA BspMI 724 ACCTGC ACCTGC(4/8) 1036 ACCTGC BsrGI 239 TGTACA T^GTACA 1084 TGTACA BstYI 162 AGATCT [GA]^GATC[CT] 350 AGATCT CfoI 49 GCGC GCG^C 73 GCGC DpnII 163 GATC ^GATC 351 GATC DraII 60 GGGTCCT [GA]G^G[ACGT]CC[CT] 582 AGGCCCC DsaI 143 CCACGG C^C[GA][CT]GG 397 CCACGG EcoO109I 60 GGGTCCT [GA]G^G[ACGT]CC[CT] 582 AGGCCCC EcoT22I 390 ATGCAT ATGCA^T 630 ATGCAT HapII 58 CCGG C^CGG 101 CCGG HgaI 118 GACGC GACGC(5/10) 130 GACGC HhaI 49 GCGC GCG^C 73 GCGC HinP1I 49 GCGC G^CGC 73 GCGC HpaII 58 CCGG C^CGG 101 CCGG MaeII 550 ACGT A^CGT 678 ACGT MboI 163 GATC ^GATC 351 GATC MflI 162 AGATCT [GA]^GATC[CT] 350 AGATCT MseI 455 TTAA T^TAA 1111 TTAA MspI 58 CCGG C^CGG 101 CCGG NciI 57 CCCGG CC^[GC]GG 58 CCGGG NdeII 163 GATC ^GATC 351 GATC NruI 66 TCGCGA TCG^CGA 437 TCGCGA NsiI 390 ATGCAT ATGCA^T 630 ATGCAT PvuII 906 CAGCTG CAG^CTG 1143 CAGCTG Sau3AI 163 GATC ^GATC 351 GATC SmlI 300 CTCAAG C^T[CT][GA]AG 578 CTCAAG SspBI 239 TGTACA T^GTACA 1084 TGTACA TaqI 95 TCGA T^CGA 107 TCGA Tru9I 455 TTAA T^TAA 1111 TTAA TthHB8I 95 TCGA T^CGA 107 TCGA XhoII 162 AGATCT [GA]^GATC[CT] 350 AGATCT ######################################### # 3 OR MORE CUT ENZYMES ( 47 enzymes) # ######################################### AccII AciI AfaI AluI AvaII BbvI BsaJI BsiYI BslI BsrI BssKI BstF5I BstNI BstUI Cac8I Cfr13I DdeI EarI EcoRII FauI Fnu4HI FokI HaeIII HinfI ItaI Ksp632I MaeIII MboII MnlI MslI MspA1I MvaI MvnI MwoI NlaIII NlaIV NspI PleI PstI RsaI Sau96I ScrFI SfaNI SfcI TseI Tsp509I TspRI