(Circular) (Six-base) MAPSORT of: pFN21AE1426.seq Check: 5160 from: 1 to: 5962 pFN21AE1426 5962 bp With 182 enzymes: SgfI * August 16, 2010 14:46 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3772 278 Size: 53 83 186 936 2236 2468 Fragments arranged by size: 2468 2236 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2481 3305 1937 Size: 544 824 4594 Fragments arranged by size: 4594 824 544 Acc65I G'GTAC_C Cuts at: 3283 3283 Size: 5962 AclI AA'CG_TT Cuts at: 1563 4089 4462 1563 Size: 2526 373 3063 Fragments arranged by size: 3063 2526 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 4025 5409 5677 1632 Size: 2393 1384 268 1917 Fragments arranged by size: 2393 1917 1384 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5765 AflIII A'CryG_T Cuts at: 1203 2745 4861 5838 1203 Size: 1542 2116 977 1327 Fragments arranged by size: 2116 1542 1327 977 AgeI A'CCGG_T Cuts at: 4793 4793 Size: 5962 AhdI GACnn_n'nnGTC Cuts at: 4691 4691 Size: 5962 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 5962 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 5962 AlwNI CAG_nnn'CTG Cuts at: 3705 5277 3705 Size: 1572 4390 ApaLI G'TGCA_C Cuts at: 4019 5175 4019 Size: 1156 4806 ApoI r'AATT_y Cuts at: 1493 2327 3002 3271 3497 3649 5758 1493 Size: 834 675 269 226 152 2109 1697 Fragments arranged by size: 2109 1697 834 675 269 226 152 AseI AT'TA_AT Cuts at: 160 1032 4516 160 Size: 872 3484 1606 Fragments arranged by size: 3484 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5962 AvaI C'yCGr_G Cuts at: 1870 1943 2062 3287 1870 Size: 73 119 1225 4545 Fragments arranged by size: 4545 1225 119 73 BamHI G'GATC_C Cuts at: 3292 3659 3292 Size: 367 5595 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2077 3283 4738 618 Size: 325 217 627 290 1206 1455 1842 Fragments arranged by size: 1842 1455 1206 627 325 290 217 BanII G_rGCy'C Cuts at: 1391 2133 3281 1391 Size: 742 1148 4072 Fragments arranged by size: 4072 1148 742 BbeI G_GCGC'C Cuts at: 2081 2081 Size: 5962 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 928 Size: 971 4991 BbvCI CC'TCA_GC Cuts at: 2546 2546 Size: 5962 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 1987 2021 4153 4187 1903 Size: 34 50 34 2132 34 3678 Fragments arranged by size: 3678 2132 50 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3854 5064 3854 Size: 1210 4752 BclI T'GATC_A Cuts at: 1548 1548 Size: 5962 BfrBI ATG'CAT Cuts at: 2397 2637 2397 Size: 240 5722 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 4573 136 Size: 107 122 71 631 605 2901 1525 Fragments arranged by size: 2901 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 2167 2355 5957 2167 Size: 188 3602 2172 Fragments arranged by size: 3602 2172 188 BlpI GC'TnA_GC Cuts at: 3379 3379 Size: 5962 Bme1580I G_kGCm'C Cuts at: 1790 4023 5179 1790 Size: 2233 1156 2573 Fragments arranged by size: 2573 2233 1156 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 5962 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3693 4651 442 Size: 416 53 83 399 24 2276 958 1753 Fragments arranged by size: 2276 1753 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5962 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2406 2406 Size: 5962 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2192 2829 4622 1239 Size: 953 637 1793 2579 Fragments arranged by size: 2579 1793 953 637 Bpu10I CC'TnA_GC Cuts at: 2258 2546 2778 2258 Size: 288 232 5442 Fragments arranged by size: 5442 288 232 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2290 2568 4087 4952 5250 2290 Size: 278 1519 865 298 3002 Fragments arranged by size: 3002 1519 865 298 278 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2864 4625 882 Size: 617 1365 1761 2219 Fragments arranged by size: 2219 1761 1365 617 BsaAI yAC'GTr Cuts at: 493 1204 3753 493 Size: 711 2549 2702 Fragments arranged by size: 2702 2549 711 BsaBI GATnn'nnATC Cuts at: 5956 5956 Size: 5962 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2078 3769 4151 4786 Size: 53 83 186 936 545 1691 382 635 Cuts at: 4786 275 Size: 1451 Fragments arranged by size: 1691 1451 936 635 545 382 186 83 53 BsaWI w'CCGG_w Cuts at: 2105 4395 4793 5067 5214 5625 2105 Size: 2290 398 274 147 411 2442 Fragments arranged by size: 2442 2290 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2165 2195 1178 Size: 30 165 30 762 30 4945 Fragments arranged by size: 4945 762 165 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2189 1186 Size: 1003 4959 BseYI C'CCAG_C Cuts at: 2014 2978 5165 5661 2014 Size: 964 2187 496 2315 Fragments arranged by size: 2315 2187 964 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 1163 Size: 573 5389 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3412 4173 5201 1540 Size: 275 186 1411 761 1028 2301 Fragments arranged by size: 2301 1411 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 4023 4108 5179 4023 Size: 85 1071 4806 Fragments arranged by size: 4806 1071 85 BsmI GAATG_Cn' Cuts at: 3473 3566 3473 Size: 93 5869 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2133 3281 4023 4108 5179 1391 Size: 399 343 1148 742 85 1071 2174 Fragments arranged by size: 2174 1148 1071 742 399 343 85 BspEI T'CCGG_A Cuts at: 2105 5625 2105 Size: 3520 2442 BspHI T'CATG_A Cuts at: 2211 3851 5581 2211 Size: 1640 1730 2592 Fragments arranged by size: 2592 1730 1640 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2738 3050 3317 844 Size: 1894 312 267 3489 Fragments arranged by size: 3489 1894 312 267 BsrBI CCG'CTC Cuts at: 1394 1823 2894 3849 1394 Size: 429 1071 955 3507 Fragments arranged by size: 3507 1071 955 429 BsrDI GCAATG_nn' Cuts at: 65 1248 4457 4631 65 Size: 1183 3209 174 1396 Fragments arranged by size: 3209 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 4606 4793 1700 Size: 110 2796 187 2869 Fragments arranged by size: 2869 2796 187 110 BsrGI T'GTAC_A Cuts at: 96 2244 3089 96 Size: 2148 845 2969 Fragments arranged by size: 2969 2148 845 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 5962 BssSI C'ACGA_G Cuts at: 2536 4022 5034 2536 Size: 1486 1012 3464 Fragments arranged by size: 3464 1486 1012 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 5962 BstBI TT'CG_AA Cuts at: 4766 4766 Size: 5962 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 5962 BstXI CCAn_nnnn'nTGG Cuts at: 2235 2235 Size: 5962 BstYI r'GATC_y Cuts at: 1972 2167 2355 3292 3659 4044 4061 5513 5599 Size: 195 188 937 367 385 17 1452 86 Cuts at: 5599 5611 5957 1972 Size: 12 346 1977 Fragments arranged by size: 1977 1452 937 385 367 346 195 188 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2148 2402 513 Size: 552 1083 254 4073 Fragments arranged by size: 4073 1083 552 254 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5223 Fragments arranged by size: 5223 683 56 BtsI GCAGTG_nn' Cuts at: 2525 3478 4272 4292 2525 Size: 953 794 20 4195 Fragments arranged by size: 4195 953 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5962 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5927 DraI TTT'AAA Cuts at: 2461 3266 2461 Size: 805 5157 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4969 809 Size: 507 3653 1802 Fragments arranged by size: 3653 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 3161 3409 4298 Size: 54 1425 50 275 18 1331 248 889 Cuts at: 4298 5927 8 Size: 1629 43 Fragments arranged by size: 1629 1425 1331 889 275 248 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3409 1537 Size: 275 1597 4090 Fragments arranged by size: 4090 1597 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2310 2862 2978 3892 1353 Size: 957 552 116 914 3423 Fragments arranged by size: 3423 957 914 552 116 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4549 4923 5069 387 Size: 1064 138 2960 374 146 1280 Fragments arranged by size: 2960 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2192 2829 4025 4622 5409 5677 1239 Size: 393 560 637 1196 597 787 268 1524 Fragments arranged by size: 1524 1196 787 637 597 560 393 268 EcoNI CCTnn'n_nnAGG Cuts at: 2777 2777 Size: 5962 EcoO109I rG'GnC_Cy Cuts at: 2066 2588 2066 Size: 522 5440 EcoRI G'AATT_C Cuts at: 3271 3649 3271 Size: 378 5584 FspI TGC'GCA Cuts at: 4468 4468 Size: 5962 HaeII r_GCGC'y Cuts at: 1678 2081 5109 1678 Size: 403 3028 2531 Fragments arranged by size: 3028 2531 403 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2100 2132 2927 2959 4603 Size: 32 135 32 528 32 795 32 1644 Cuts at: 4603 4635 4677 4709 1373 Size: 32 42 32 2626 Fragments arranged by size: 2626 1644 795 528 135 42 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3306 3552 669 Size: 988 281 1368 246 3079 Fragments arranged by size: 3079 1368 988 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 5962 HpaI GTT'AAC Cuts at: 3552 3552 Size: 5962 KasI G'GCGC_C Cuts at: 2077 2077 Size: 5962 KpnI G_GTAC'C Cuts at: 3287 3287 Size: 5962 MfeI C'AATT_G Cuts at: 3561 3561 Size: 5962 MluI A'CGCG_T Cuts at: 5838 5838 Size: 5962 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 5076 5260 1305 Size: 3771 184 2007 Fragments arranged by size: 3771 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 4140 Fragments arranged by size: 4140 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2950 3022 3106 3920 4279 4438 518 Size: 648 1784 72 84 814 359 159 2042 Fragments arranged by size: 2042 1784 814 648 359 159 84 72 MspA1I CmG'CkG Cuts at: 1606 2051 2913 3150 3378 4055 5203 5448 1606 Size: 445 862 237 228 677 1148 245 2120 Fragments arranged by size: 2120 1148 862 677 445 245 237 228 NarI GG'CG_CC Cuts at: 2078 2078 Size: 5962 NcoI C'CATG_G Cuts at: 513 1065 513 Size: 552 5410 NdeI CA'TA_TG Cuts at: 387 387 Size: 5962 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5962 NotI GC'GGCC_GC Cuts at: 3409 3409 Size: 5962 NruI TCG'CGA Cuts at: 2073 2444 2073 Size: 371 5591 NsiI A_TGCA'T Cuts at: 2399 2639 2399 Size: 240 5722 NspI r_CATG'y Cuts at: 1132 1229 2401 2641 2749 3021 3147 3320 4865 Size: 97 1172 240 108 272 126 173 1545 Cuts at: 4865 1132 Size: 2229 Fragments arranged by size: 2229 1545 1172 272 240 173 126 108 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 5962 PciI A'CATG_T Cuts at: 2745 4861 2745 Size: 2116 3846 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 5962 PfoI T'CCnGG_A Cuts at: 3067 3067 Size: 5962 PmeI GTTT'AAAC Cuts at: 3266 3266 Size: 5962 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4031 4063 5544 5576 4031 Size: 32 1481 32 4417 Fragments arranged by size: 4417 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2066 2066 Size: 5962 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5962 PsiI TTA'TAA Cuts at: 2346 3532 2346 Size: 1186 4776 PstI C_TGCA'G Cuts at: 830 2338 2520 2768 2891 3314 830 Size: 1508 182 248 123 423 3478 Fragments arranged by size: 3478 1508 423 248 182 123 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5962 PvuII CAG'CTG Cuts at: 2913 3150 2913 Size: 237 5725 SalI G'TCGA_C Cuts at: 1936 3304 1936 Size: 1368 4594 SbfI CC_TGCA'GG Cuts at: 3314 3314 Size: 5962 ScaI AGT'ACT Cuts at: 4210 5922 4210 Size: 1712 4250 SfcI C'TryA_G Cuts at: 826 1045 2334 2482 2516 2764 2887 3310 4445 Size: 219 1289 148 34 248 123 423 1135 Cuts at: 4445 5126 5317 826 Size: 681 191 1471 Fragments arranged by size: 1471 1289 1135 681 423 248 219 191 148 123 34 SfoI GGC'GCC Cuts at: 2079 2079 Size: 5962 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5962 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5962 SmaI CCC'GGG Cuts at: 1872 2064 3289 1872 Size: 192 1225 4545 Fragments arranged by size: 4545 1225 192 SmlI C'TyrA_G Cuts at: 820 1017 1943 2305 2583 4066 4967 5229 820 Size: 197 926 362 278 1483 901 262 1553 Fragments arranged by size: 1553 1483 926 901 362 278 262 197 SnaBI TAC'GTA Cuts at: 493 3753 493 Size: 3260 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5962 SphI G_CATG'C Cuts at: 1132 2401 3320 1132 Size: 1269 919 3774 Fragments arranged by size: 3774 1269 919 SspI AAT'ATT Cuts at: 5 52 2234 3886 5 Size: 47 2182 1652 2081 Fragments arranged by size: 2182 2081 1652 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 5962 StyI C'CwwG_G Cuts at: 513 1065 3401 513 Size: 552 2336 3074 Fragments arranged by size: 3074 2336 552 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 3989 4006 4159 4344 4789 325 Size: 1255 2409 17 153 185 445 1498 Fragments arranged by size: 2409 1498 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2218 2244 3089 Size: 274 80 33 51 1443 241 26 845 Cuts at: 3089 4208 5920 96 Size: 1119 1712 138 Fragments arranged by size: 1712 1443 1119 845 274 241 138 80 51 33 26 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 5922 5954 5922 Size: 32 5930 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 5962 XbaI T'CTAG_A Cuts at: 3298 3298 Size: 5962 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2011 2840 2011 Size: 829 5133 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5962 XmaI C'CCGG_G Cuts at: 1870 2062 3287 1870 Size: 192 1225 4545 Fragments arranged by size: 4545 1225 192 XmnI GAAnn'nnTTC Cuts at: 4091 4091 Size: 5962 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3770 276 Size: 53 83 186 936 2236 2468 Fragments arranged by size: 2468 2236 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI BbvCI BcgI BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoNI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NarI NcoI NdeI NheI NotI NruI NsiI NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PstI PvuI PvuII SalI SbfI ScaI SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI ApaI AscI AvrII BaeI BsiWI BsmBI BstZ17I Bsu36I DraIII EcoICRI EcoRV FalI FseI FspAI NaeI NgoMIV PacI PmlI PspOMI PspXI PsrI RsrII SacI SacII SanDI SapI SexAI SfiI SrfI SwaI