################################## # NO CUT ENZYMES ( 113 enzymes) # ################################## AatII Acc65I AccI AccIII AclI AcyI AflII AgeI AhdI AocI ApaI AscI Asp718I AspEI AspI AviII AvrII BalI BamHI BanII BbeI BbrPI BbvCI BciVI BfrI BglI BlnI BsaAI BsaBI BsaHI BsaI BsaWI BseAI BsiEI BsiWI BsmBI BspDI BspEI BspHI BspMI BsrBI BssSI Bst1107I BstEII BstPI BstXI BstZ17I Bsu36I ClaI CpoI DrdI EaeI EagI Eam1105I EclXI Eco52I Eco81I EcoNI EcoO65I EcoRI FauI FseI FspI Hin1I HpaI KasI KpnI KspI MamI MfeI MluI MluNI MroI MscI MunI NaeI NarI NgoMIV NheI NotI NruI PacI PaeR7I PflFI PinAI PmaCI PmeI PmlI PshAI Psp1406I PstI PvuI RcaI RsrII SacI SacII SalI SbfI ScaI SexAI SfcI SfiI SfoI SgrAI SmaI SnaBI SpeI Sse8387I SwaI Tth111I XhoI XmaCI XmaI ################################### # ONE CUT ENZYMES ( 36 enzymes) # ################################### Alw44I 1633 GTGCAC G^TGCAC AlwNI 1232 CAGGACCTG CAG[ACGT][ACGT][ACGT]^CTG Aor51HI 361 AGCGCT AGC^GCT ApaLI 1633 GTGCAC G^TGCAC Asp700I 1409 GAATGACTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC AspHI 1633 GTGCAC G[AT]GC[AT]^C BanI 2160 GGTGCC G^G[CT][GA]CC BcgI 2063 CGAAAAATTTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10) BcnI 1405 CCGGG CC^[GC]GG BglII 1937 AGATCT A^GATCT BlpI 918 GCTTAGC GC^T[ACGT]AGC BmyI 1633 GTGCAC G[AGT]GC[ACT]^C Bpu1102I 918 GCTTAGC GC^T[ACGT]AGC BsiHKAI 1633 GTGCAC G[AT]GC[AT]^C BsmFI 2521 GTCCC GGGAC(10/14) Bsp1286I 1633 GTGCAC G[AGT]GC[ACT]^C BsrFI 1349 ACCGGC [GA]^CCGG[CT] BssHII 52 GCGCGC G^CGCGC CelII 918 GCTTAGC GC^T[ACGT]AGC Cfr10I 1349 ACCGGC [GA]^CCGG[CT] DraIII 2525 CACATGGTG CAC[ACGT][ACGT][ACGT]^GTG Eco47III 361 AGCGCT AGC^GCT EcoRV 1657 GATATC GAT^ATC HaeII 361 AGCGCT [GA]GCGC^[CT] HgaI 33 GACGC GACGC(5/10) HincII 370 GTCAAC GT[CT]^[GA]AC HindII 370 GTCAAC GT[CT]^[GA]AC NciI 1405 CCGGG CC^[GC]GG NdeI 2293 CATATG CA^TATG PleI 1150 GACTC GAGTC(4/5) SapI 951 GAAGAGC GCTCTTC(1/4) SphI 2360 GCATGC GCATG^C SspI 1975 AATATT AAT^ATT StuI 390 AGGCCT AGG^CCT XbaI 2109 TCTAGA T^CTAGA XmnI 1409 GAATGACTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC ################################### # TWO CUT ENZYMES ( 38 enzymes) # ################################### AseI 99 ATTAAT AT^TAAT 741 ATTAAT AsnI 99 ATTAAT AT^TAAT 741 ATTAAT AvaI 70 CCCGAG C^[CT]CG[GA]G 756 CCCGAG BbsI 796 GAAGAC GAAGAC(2/6) 2461 GAAGAC BclI 120 TGATCA T^GATCA 1905 TGATCA BpmI 1229 CTCCAG CTGGAG(16/14) 829 CTGGAG BpuAI 796 GAAGAC GAAGAC(2/6) 2461 GAAGAC BsoBI 70 CCCGAG C^[CT]CG[GA]G 756 CCCGAG Bsp1407I 295 TGTACA T^GTACA 708 TGTACA BspLU11I 812 ACATGT A^CATGT 1379 ACATGT BsrGI 295 TGTACA T^GTACA 708 TGTACA BstAPI 540 GCAGTGCTTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC 1717 GCAAATTGTGC BstBI 992 TTCGAA TT^CGAA 2240 TTCGAA DraI 1458 TTTAAA TTT^AAA 1570 TTTAAA DraII 1233 AGGACCT [GA]G^G[ACGT]CC[CT] 2519 GGGTCCC EarI 951 GAAGAG CTCTTC(1/4) 2155 GAAGAG EcoO109I 1233 AGGACCT [GA]G^G[ACGT]CC[CT] 2519 GGGTCCC EcoT14I 485 CCATGG C^C[AT][AT]GG 1758 CCATGG FbaI 120 TGATCA T^GATCA 1905 TGATCA HapII 1350 CCGG C^CGG 1405 CCGG HindIII 198 AAGCTT A^AGCTT 1370 AAGCTT HpaII 1350 CCGG C^CGG 1405 CCGG HphI 753 TCACC GGTGA(8/7) 1417 TCACC Ksp632I 951 GAAGAG CTCTTC(1/4) 2155 GAAGAG MslI 457 CATGTCAGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG 2628 CATCAACATG MspA1I 1856 CAGCTG C[AC]G^C[GT]G 1966 CAGCTG MspI 1350 CCGG C^CGG 1405 CCGG NcoI 485 CCATGG C^CATGG 1758 CCATGG NspV 992 TTCGAA TT^CGAA 2240 TTCGAA PpuMI 1233 AGGACCT [GA]G^G[AT]CC[CT] 2519 GGGTCCC PshBI 99 ATTAAT AT^TAAT 741 ATTAAT PvuII 1856 CAGCTG CAG^CTG 1966 CAGCTG SfuI 992 TTCGAA TT^CGAA 2240 TTCGAA SspBI 295 TGTACA T^GTACA 708 TGTACA StyI 485 CCATGG C^C[AT][AT]GG 1758 CCATGG TaqI 993 TCGA T^CGA 2241 TCGA TthHB8I 993 TCGA T^CGA 2241 TCGA XcmI 1851 CCAGTCAGCTGATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG 2431 CCAGTTATTCAGTGG ######################################### # 3 OR MORE CUT ENZYMES ( 71 enzymes) # ######################################### AccII AciI AcsI AfaI AflIII AluI AlwI ApoI AvaII BbvI BfaI BsaJI BseRI BsgI BsiYI BslI BsmAI BsmI BsrDI BsrI BssKI BstF5I BstNI BstUI BstYI Cac8I CfoI Cfr13I DdeI DpnII DsaI EcoRII EcoT22I Fnu4HI FokI HaeIII HhaI HinP1I HinfI ItaI MaeI MaeII MaeIII MboI MboII MflI MnlI MseI MvaI MvnI MwoI NdeII NlaIII NlaIV NsiI NspI PflMI RsaI Sau3AI Sau96I ScrFI SfaNI SmlI TfiI Tru9I TseI Tsp45I Tsp509I TspRI Van91I XhoII