(Linear) (Six-base) MAPSORT of: KIEE3922.seq Check: 3713 from: 1 to: 2727 KIEE3922.orf With 182 enzymes: SgfI * March 22, 2012 16:51 .. AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 0 908 2727 Size: 908 1819 AfeI AGC'GCT Cuts at: 0 363 2727 Size: 363 2364 AflIII A'CryG_T Cuts at: 0 186 812 1379 2727 Size: 186 626 567 1348 Fragments arranged by size: 1348 626 567 186 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 0 1760 2650 2727 Size: 1760 890 77 Fragments arranged by size: 1760 890 77 AlwNI CAG_nnn'CTG Cuts at: 0 1237 2727 Size: 1237 1490 ApaLI G'TGCA_C Cuts at: 0 1633 2727 Size: 1633 1094 ApoI r'AATT_y Cuts at: 0 153 160 1432 2067 2727 Size: 153 7 1272 635 660 Fragments arranged by size: 1272 660 635 153 7 AseI AT'TA_AT Cuts at: 0 100 742 2727 Size: 100 642 1985 Fragments arranged by size: 1985 642 100 AvaI C'yCGr_G Cuts at: 0 70 756 2727 Size: 70 686 1971 Fragments arranged by size: 1971 686 70 BanI G'GyrC_C Cuts at: 0 2160 2727 Size: 2160 567 BbsI GAAGACnn'nnnn_ Cuts at: 0 803 2468 2727 Size: 803 1665 259 Fragments arranged by size: 1665 803 259 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 0 2052 2086 2727 Size: 2052 34 641 Fragments arranged by size: 2052 641 34 BclI T'GATC_A Cuts at: 0 120 1905 2727 Size: 120 1785 822 Fragments arranged by size: 1785 822 120 BfrBI ATG'CAT Cuts at: 0 178 1548 1840 2627 2727 Size: 178 1370 292 787 100 Fragments arranged by size: 1370 787 292 178 100 BglII A'GATC_T Cuts at: 0 1937 2727 Size: 1937 790 BlpI GC'TnA_GC Cuts at: 0 919 2727 Size: 919 1808 Bme1580I G_kGCm'C Cuts at: 0 1637 2727 Size: 1637 1090 BmgBI CAC'GTC Cuts at: 0 187 987 2727 Size: 187 800 1740 Fragments arranged by size: 1740 800 187 BmrI ACTGGGnnnn_n' Cuts at: 0 1007 1845 2727 Size: 1007 838 882 Fragments arranged by size: 1007 882 838 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 0 2506 2727 Size: 2506 221 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 850 1214 2727 Size: 850 364 1513 Fragments arranged by size: 1513 850 364 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 2456 2623 2656 2727 Size: 2456 167 33 71 Fragments arranged by size: 2456 167 71 33 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 0 823 853 1683 1713 2727 Size: 823 30 830 30 1014 Fragments arranged by size: 1014 830 823 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 0 671 683 2491 2727 Size: 671 12 1808 236 Fragments arranged by size: 1808 671 236 12 BseYI C'CCAG_C Cuts at: 0 1930 2727 Size: 1930 797 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 0 472 559 1334 2727 Size: 472 87 775 1393 Fragments arranged by size: 1393 775 472 87 BsiHKAI G_wGCw'C Cuts at: 0 1637 2727 Size: 1637 1090 BsmI GAATG_Cn' Cuts at: 0 178 241 1605 2727 Size: 178 63 1364 1122 Fragments arranged by size: 1364 1122 178 63 Bsp1286I G_dGCh'C Cuts at: 0 1637 2727 Size: 1637 1090 BsrDI GCAATG_nn' Cuts at: 0 209 2080 2156 2727 Size: 209 1871 76 571 Fragments arranged by size: 1871 571 209 76 BsrFI r'CCGG_y Cuts at: 0 1349 2727 Size: 1349 1378 BsrGI T'GTAC_A Cuts at: 0 295 708 2727 Size: 295 413 2019 Fragments arranged by size: 2019 413 295 BssHII G'CGCG_C Cuts at: 0 52 2727 Size: 52 2675 BstAPI GCAn_nnn'nTGC Cuts at: 0 546 1723 2727 Size: 546 1177 1004 Fragments arranged by size: 1177 1004 546 BstBI TT'CG_AA Cuts at: 0 993 2241 2727 Size: 993 1248 486 Fragments arranged by size: 1248 993 486 BstYI r'GATC_y Cuts at: 0 641 1937 2427 2727 Size: 641 1296 490 300 Fragments arranged by size: 1296 641 490 300 BtgI C'CryG_G Cuts at: 0 59 485 1758 2727 Size: 59 426 1273 969 Fragments arranged by size: 1273 969 426 59 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 0 25 2727 Size: 25 2702 BtsI GCAGTG_nn' Cuts at: 0 499 547 2727 Size: 499 48 2180 Fragments arranged by size: 2180 499 48 DraI TTT'AAA Cuts at: 0 1460 1572 2727 Size: 1460 112 1155 Fragments arranged by size: 1460 1155 112 DraIII CAC_nnn'GTG Cuts at: 0 2530 2727 Size: 2530 197 EarI CTCTTCn'nnn_ Cuts at: 0 946 2150 2727 Size: 946 1204 577 Fragments arranged by size: 1204 946 577 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 0 441 2727 Size: 441 2286 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 0 850 908 1214 2727 Size: 850 58 306 1513 Fragments arranged by size: 1513 850 306 58 EcoO109I rG'GnC_Cy Cuts at: 0 1234 2520 2727 Size: 1234 1286 207 Fragments arranged by size: 1286 1234 207 EcoRV GAT'ATC Cuts at: 0 1659 2727 Size: 1659 1068 HaeII r_GCGC'y Cuts at: 0 365 2727 Size: 365 2362 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 0 823 855 1404 1436 1519 1551 1795 1812 Size: 823 32 549 32 83 32 244 17 Cuts at: 1812 1827 1844 2026 2058 2727 Size: 15 17 182 32 669 Fragments arranged by size: 823 669 549 244 182 83 32 32 32 32 17 17 15 HincII GTy'rAC Cuts at: 0 372 2727 Size: 372 2355 HindIII A'AGCT_T Cuts at: 0 198 1370 2727 Size: 198 1172 1357 Fragments arranged by size: 1357 1172 198 MslI CAynn'nnrTG Cuts at: 0 461 2632 2727 Size: 461 2171 95 Fragments arranged by size: 2171 461 95 MspA1I CmG'CkG Cuts at: 0 1858 1968 2727 Size: 1858 110 759 Fragments arranged by size: 1858 759 110 NcoI C'CATG_G Cuts at: 0 485 1758 2727 Size: 485 1273 969 Fragments arranged by size: 1273 969 485 NdeI CA'TA_TG Cuts at: 0 2294 2727 Size: 2294 433 NsiI A_TGCA'T Cuts at: 0 180 1550 1842 2629 2727 Size: 180 1370 292 787 98 Fragments arranged by size: 1370 787 292 180 98 NspI r_CATG'y Cuts at: 0 460 508 816 1383 2364 2627 2637 2727 Size: 460 48 308 567 981 263 10 90 Fragments arranged by size: 981 567 460 308 263 90 48 10 PciI A'CATG_T Cuts at: 0 812 1379 2727 Size: 812 567 1348 Fragments arranged by size: 1348 812 567 PflMI CCAn_nnn'nTGG Cuts at: 0 1237 2037 2530 2727 Size: 1237 800 493 197 Fragments arranged by size: 1237 800 493 197 PfoI T'CCnGG_A Cuts at: 0 1230 1404 2727 Size: 1230 174 1323 Fragments arranged by size: 1323 1230 174 PpuMI rG'GwC_Cy Cuts at: 0 1234 2520 2727 Size: 1234 1286 207 Fragments arranged by size: 1286 1234 207 PvuII CAG'CTG Cuts at: 0 1858 1968 2727 Size: 1858 110 759 Fragments arranged by size: 1858 759 110 SanDI GG'GwC_CC Cuts at: 0 2520 2727 Size: 2520 207 SapI GCTCTTCn'nnn_ Cuts at: 0 946 2727 Size: 946 1781 SmlI C'TyrA_G Cuts at: 0 2471 2638 2671 2727 Size: 2471 167 33 56 Fragments arranged by size: 2471 167 56 33 SphI G_CATG'C Cuts at: 0 2364 2727 Size: 2364 363 SspI AAT'ATT Cuts at: 0 1977 2727 Size: 1977 750 StuI AGG'CCT Cuts at: 0 392 2727 Size: 392 2335 StyI C'CwwG_G Cuts at: 0 485 1758 2727 Size: 485 1273 969 Fragments arranged by size: 1273 969 485 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 0 2539 2727 Size: 2539 188 TatI w'GTAC_w Cuts at: 0 295 602 708 2727 Size: 295 307 106 2019 Fragments arranged by size: 2019 307 295 106 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 0 1683 1715 2727 Size: 1683 32 1012 Fragments arranged by size: 1683 1012 32 XbaI T'CTAG_A Cuts at: 0 2109 2727 Size: 2109 618 XcmI CCAnnnn_n'nnnnTGG Cuts at: 0 1858 2438 2727 Size: 1858 580 289 Fragments arranged by size: 1858 580 289 XmnI GAAnn'nnTTC Cuts at: 0 1413 2727 Size: 1413 1314 Enzymes that do cut: AcuI AfeI AflIII AlfI AlwNI ApaLI ApoI AseI AvaI BanI BbsI BcgI BclI BfrBI BglII BlpI Bme1580I BmgBI BmrI BplI BpmI BpuEI BsaXI BseRI BseYI BsgI BsiHKAI BsmI Bsp1286I BsrDI BsrFI BsrGI BssHII BstAPI BstBI BstYI BtgI BtgZI BtsI DraI DraIII EarI EciI Eco57MI EcoO109I EcoRV HaeII Hin4I HincII HindIII MslI MspA1I NcoI NdeI NsiI NspI PciI PflMI PfoI PpuMI PvuII SanDI SapI SmlI SphI SspI StuI StyI TaqII TatI TstI XbaI XcmI XmnI Enzymes that do not cut: AarI AatII AccI Acc65I AclI AflII AgeI AhdI AleI AloI ApaI AscI AsiSI AvrII BaeI BamHI BanII BbeI BbvCI BciVI BglI BmtI Bpu10I BsaI BsaAI BsaBI BsaHI BsaWI BsiEI BsiWI BsmBI BspEI BspHI BspMI BsrBI BssSI BstEII BstXI BstZ17I Bsu36I ClaI CspCI DrdI EaeI EagI EcoICRI EcoNI EcoRI FalI FseI FspI FspAI HpaI KasI KpnI MfeI MluI MmeI MscI NaeI NarI NgoMIV NheI NotI NruI PacI PasI PmeI PmlI PpiI PshAI PsiI PspOMI PspXI PsrI PstI PvuI RsrII SacI SacII SalI SbfI ScaI SexAI SfcI SfiI SfoI SgfI SgrAI SmaI SnaBI SpeI SrfI SwaI Tth111I XhoI XmaI ZraI