(Linear) (Six-base) MAPSORT of: KIEE5618.seq Check: 1589 from: 1 to: 1599 KIEE5618.orf With 182 enzymes: SgfI * December 5, 2014 13:03 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 0 1054 1599 Size: 1054 545 AatII G_ACGT'C Cuts at: 0 10 1599 Size: 10 1589 Acc65I G'GTAC_C Cuts at: 0 999 1599 Size: 999 600 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 0 137 290 1526 1599 Size: 137 153 1236 73 Fragments arranged by size: 1236 153 137 73 AflIII A'CryG_T Cuts at: 0 1588 1599 Size: 1588 11 AlwNI CAG_nnn'CTG Cuts at: 0 250 1599 Size: 250 1349 ApaLI G'TGCA_C Cuts at: 0 1067 1599 Size: 1067 532 ApoI r'AATT_y Cuts at: 0 208 644 843 1031 1599 Size: 208 436 199 188 568 Fragments arranged by size: 568 436 208 199 188 AseI AT'TA_AT Cuts at: 0 1075 1599 Size: 1075 524 AvaI C'yCGr_G Cuts at: 0 1388 1599 Size: 1388 211 BaeI ACnnnnGTAyCnnnnnnn_nnnnn' Cuts at: 0 1322 1355 1599 Size: 1322 33 244 Fragments arranged by size: 1322 244 33 BanI G'GyrC_C Cuts at: 0 999 1599 Size: 999 600 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 0 28 1599 Size: 28 1571 BciVI GTATCCnnnnn_n' Cuts at: 0 282 1599 Size: 282 1317 BglII A'GATC_T Cuts at: 0 465 1599 Size: 465 1134 Bme1580I G_kGCm'C Cuts at: 0 917 1071 1599 Size: 917 154 528 Fragments arranged by size: 917 528 154 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 188 849 1599 Size: 188 661 750 Fragments arranged by size: 750 661 188 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 171 1599 Size: 171 1428 BsaI GGTCTCn'nnnn_ Cuts at: 0 395 938 1599 Size: 395 543 661 Fragments arranged by size: 661 543 395 BsaAI yAC'GTr Cuts at: 0 1521 1599 Size: 1521 78 BsaHI Gr'CG_yC Cuts at: 0 7 1599 Size: 7 1592 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 0 954 984 1599 Size: 954 30 615 Fragments arranged by size: 954 615 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 0 1373 1376 1599 Size: 1373 3 223 Fragments arranged by size: 1373 223 3 BseYI C'CCAG_C Cuts at: 0 365 1206 1599 Size: 365 841 393 Fragments arranged by size: 841 393 365 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 0 1421 1599 Size: 1421 178 BsiHKAI G_wGCw'C Cuts at: 0 1071 1305 1599 Size: 1071 234 294 Fragments arranged by size: 1071 294 234 BsmBI CGTCTCn'nnnn_ Cuts at: 0 14 1599 Size: 14 1585 Bsp1286I G_dGCh'C Cuts at: 0 917 1071 1305 1599 Size: 917 154 234 294 Fragments arranged by size: 917 294 234 154 BspMI ACCTGCnnnn'nnnn_ Cuts at: 0 154 987 1054 1599 Size: 154 833 67 545 Fragments arranged by size: 833 545 154 67 BsrDI GCAATG_nn' Cuts at: 0 998 1599 Size: 998 601 BsrGI T'GTAC_A Cuts at: 0 91 1599 Size: 91 1508 BstYI r'GATC_y Cuts at: 0 39 465 1599 Size: 39 426 1134 Fragments arranged by size: 1134 426 39 BtgI C'CryG_G Cuts at: 0 56 284 608 1326 1599 Size: 56 228 324 718 273 Fragments arranged by size: 718 324 273 228 56 BtsI GCAGTG_nn' Cuts at: 0 840 944 1442 1599 Size: 840 104 498 157 Fragments arranged by size: 840 498 157 104 DraIII CAC_nnn'GTG Cuts at: 0 1524 1599 Size: 1524 75 EaeI y'GGCC_r Cuts at: 0 348 1374 1599 Size: 348 1026 225 Fragments arranged by size: 1026 348 225 EarI CTCTTCn'nnn_ Cuts at: 0 266 1599 Size: 266 1333 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 0 137 188 290 849 1526 1599 Size: 137 51 102 559 677 73 Fragments arranged by size: 677 559 137 102 73 51 EcoNI CCTnn'n_nnAGG Cuts at: 0 150 398 1599 Size: 150 248 1201 Fragments arranged by size: 1201 248 150 EcoO109I rG'GnC_Cy Cuts at: 0 22 1599 Size: 22 1577 EcoRI G'AATT_C Cuts at: 0 208 644 1599 Size: 208 436 955 Fragments arranged by size: 955 436 208 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 0 142 1599 Size: 142 1457 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 0 757 789 910 942 1157 1189 1371 1403 Size: 757 32 121 32 215 32 182 32 Cuts at: 1403 1411 1443 1599 Size: 8 32 156 Fragments arranged by size: 757 215 182 156 121 32 32 32 32 32 8 HincII GTy'rAC Cuts at: 0 266 1231 1599 Size: 266 965 368 Fragments arranged by size: 965 368 266 HindIII A'AGCT_T Cuts at: 0 562 1599 Size: 562 1037 KpnI G_GTAC'C Cuts at: 0 1003 1599 Size: 1003 596 MfeI C'AATT_G Cuts at: 0 104 1599 Size: 104 1495 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 0 328 1117 1260 1599 Size: 328 789 143 339 Fragments arranged by size: 789 339 328 143 MscI TGG'CCA Cuts at: 0 1376 1599 Size: 1376 223 MspA1I CmG'CkG Cuts at: 0 36 1599 Size: 36 1563 NcoI C'CATG_G Cuts at: 0 284 608 1326 1599 Size: 284 324 718 273 Fragments arranged by size: 718 324 284 273 NdeI CA'TA_TG Cuts at: 0 131 1599 Size: 131 1468 NspI r_CATG'y Cuts at: 0 1592 1599 Size: 1592 7 PasI CC'CwG_GG Cuts at: 0 910 1599 Size: 910 689 PciI A'CATG_T Cuts at: 0 1588 1599 Size: 1588 11 PflMI CCAn_nnn'nTGG Cuts at: 0 1498 1524 1599 Size: 1498 26 75 Fragments arranged by size: 1498 75 26 PpuMI rG'GwC_Cy Cuts at: 0 22 1599 Size: 22 1577 PspXI vC'TCGA_Gb Cuts at: 0 1388 1599 Size: 1388 211 PstI C_TGCA'G Cuts at: 0 801 1238 1599 Size: 801 437 361 Fragments arranged by size: 801 437 361 PvuII CAG'CTG Cuts at: 0 36 1599 Size: 36 1563 SanDI GG'GwC_CC Cuts at: 0 22 1599 Size: 22 1577 SfcI C'TryA_G Cuts at: 0 251 797 1234 1599 Size: 251 546 437 365 Fragments arranged by size: 546 437 365 251 SmlI C'TyrA_G Cuts at: 0 150 1388 1599 Size: 150 1238 211 Fragments arranged by size: 1238 211 150 SpeI A'CTAG_T Cuts at: 0 1087 1581 1599 Size: 1087 494 18 Fragments arranged by size: 1087 494 18 StyI C'CwwG_G Cuts at: 0 284 608 973 1326 1599 Size: 284 324 365 353 273 Fragments arranged by size: 365 353 324 284 273 TatI w'GTAC_w Cuts at: 0 91 1599 Size: 91 1508 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 0 969 1001 1599 Size: 969 32 598 Fragments arranged by size: 969 598 32 Tth111I GACn'n_nGTC Cuts at: 0 1242 1599 Size: 1242 357 XcmI CCAnnnn_n'nnnnTGG Cuts at: 0 33 1599 Size: 33 1566 XhoI C'TCGA_G Cuts at: 0 1388 1599 Size: 1388 211 XmnI GAAnn'nnTTC Cuts at: 0 843 1599 Size: 843 756 ZraI GAC'GTC Cuts at: 0 8 1599 Size: 8 1591 Enzymes that do cut: AarI AatII Acc65I AcuI AflIII AlwNI ApaLI ApoI AseI AvaI BaeI BanI BcgI BciVI BglII Bme1580I BpmI BpuEI BsaI BsaAI BsaHI BsaXI BseRI BseYI BsgI BsiHKAI BsmBI Bsp1286I BspMI BsrDI BsrGI BstYI BtgI BtsI DraIII EaeI EarI Eco57MI EcoNI EcoO109I EcoRI FalI Hin4I HincII HindIII KpnI MfeI MmeI MscI MspA1I NcoI NdeI NspI PasI PciI PflMI PpuMI PspXI PstI PvuII SanDI SfcI SmlI SpeI StyI TatI TstI Tth111I XcmI XhoI XmnI ZraI Enzymes that do not cut: AccI AclI AfeI AflII AgeI AhdI AleI AlfI AloI ApaI AscI AsiSI AvrII BamHI BanII BbeI BbsI BbvCI BclI BfrBI BglI BlpI BmgBI BmrI BmtI BplI Bpu10I BsaBI BsaWI BsiEI BsiWI BsmI BspEI BspHI BsrBI BsrFI BssHII BssSI BstAPI BstBI BstEII BstXI BstZ17I Bsu36I BtgZI ClaI CspCI DraI DrdI EagI EciI EcoICRI EcoRV FseI FspI FspAI HaeII HpaI KasI MluI MslI NaeI NarI NgoMIV NheI NotI NruI NsiI PacI PfoI PmeI PmlI PpiI PshAI PsiI PspOMI PsrI PvuI RsrII SacI SacII SalI SapI SbfI ScaI SexAI SfiI SfoI SgfI SgrAI SmaI SnaBI SphI SrfI SspI StuI SwaI TaqII XbaI XmaI