################################## # NO CUT ENZYMES ( 101 enzymes) # ################################## AatII AccIII AcyI AflII AflIII AgeI AhdI AocI Aor51HI ApaI AscI AseI AsnI Asp700I AspEI AvrII BamHI BbeI BcgI BfrI BglI BlnI BsaHI BsaWI BseAI BsiEI BsiWI BsmBI BsmI Bsp1407I BspDI BspEI BspHI BspLU11I BsrGI BssHII BssSI Bst1107I BstBI BstEII BstPI BstZ17I Bsu36I ClaI DraI EagI Eam1105I EclXI Eco47III Eco52I Eco81I EcoNI EcoO65I EcoRI EcoRV EcoT22I FseI HaeII Hin1I HincII HindII HindIII HpaI KasI MfeI MluI MroI MunI NarI NheI NotI NruI NsiI NspV PacI PinAI PmeI PshAI PshBI PstI PvuI RcaI SalI SapI SbfI ScaI SexAI SfcI SfiI SfoI SfuI SgrAI SnaBI SpeI Sse8387I SspBI SspI StuI SwaI XbaI XmnI ################################### # ONE CUT ENZYMES ( 41 enzymes) # ################################### Acc65I 1876 GGTACC G^GTACC AccI 1453 GTCTAC GT^[AC][GT]AC AclI 2218 AACGTT AA^CGTT AcsI 821 GAATTT [GA]^AATT[CT] Alw44I 2332 GTGCAC G^TGCAC ApaLI 2332 GTGCAC G^TGCAC ApoI 821 GAATTT [GA]^AATT[CT] Asp718I 1876 GGTACC G^GTACC AspI 949 GACCCAGTC GAC[ACGT]^[ACGT][ACGT]GTC AviII 2063 TGCGCA TGC^GCA BbrPI 538 CACGTG CAC^GTG BbvCI 1486 GCTGAGG CCTCAGC(-5/-2) BciVI 271 GTATCC GTATCC(6/5) BglII 725 AGATCT A^GATCT BsgI 1834 CTGCAC GTGCAG(16/14) BstAPI 1403 GCATCTTCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC CpoI 1928 CGGACCG CG^G[AT]CCG DraIII 883 CACGGGGTG CAC[ACGT][ACGT][ACGT]^GTG DrdI 1000 GACAATGGAGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC FspI 2063 TGCGCA TGC^GCA KpnI 1876 GGTACC GGTAC^C KspI 426 CCGCGG CCGC^GG MseI 806 TTAA T^TAA NaeI 263 GCCGGC GCC^GGC NgoMIV 263 GCCGGC G^CCGGC PaeR7I 2438 CTCGAG C^TCGAG PflFI 949 GACCCAGTC GAC[ACGT]^[ACGT][ACGT]GTC PmaCI 538 CACGTG CAC^GTG PmlI 538 CACGTG CAC^GTG PpuMI 183 GGGACCT [GA]G^G[AT]CC[CT] Psp1406I 2218 AACGTT AA^CGTT PvuII 296 CAGCTG CAG^CTG RsrII 1928 CGGACCG CG^G[AT]CCG SacI 354 GAGCTC GAGCT^C SacII 426 CCGCGG CCGC^GG SphI 323 GCATGC GCATG^C Tru9I 806 TTAA T^TAA Tsp509I 822 AATT ^AATT Tth111I 949 GACCCAGTC GAC[ACGT]^[ACGT][ACGT]GTC XcmI 1895 CCAAGAAGGAAGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG XhoI 2438 CTCGAG C^TCGAG ################################### # TWO CUT ENZYMES ( 17 enzymes) # ################################### BclI 542 TGATCA T^GATCA 1799 TGATCA BfaI 328 CTAG C^TAG 1394 CTAG BsaAI 538 CACGTG [CT]AC^GT[GA] 769 TACGTG BsaBI 726 GATCTACATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC 1794 GATGCTGATC EarI 736 CTCTTC CTCTTC(1/4) 1329 GAAGAG EcoT14I 980 CCATGG C^C[AT][AT]GG 1235 CCATGG FbaI 542 TGATCA T^GATCA 1799 TGATCA Ksp632I 736 CTCTTC CTCTTC(1/4) 1329 GAAGAG MaeI 328 CTAG C^TAG 1394 CTAG MamI 726 GATCTACATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC 1794 GATGCTGATC NcoI 980 CCATGG C^CATGG 1235 CCATGG NdeI 442 CATATG CA^TATG 932 CATATG NspI 323 GCATGC [GA]CATG^[CT] 1604 ACATGC PflMI 1971 CCATCTTGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 2362 CCAGCCCCTGG SmlI 493 CTTGAG C^T[CT][GA]AG 2438 CTCGAG StyI 980 CCATGG C^C[AT][AT]GG 1235 CCATGG Van91I 1971 CCATCTTGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 2362 CCAGCCCCTGG ######################################### # 3 OR MORE CUT ENZYMES ( 99 enzymes) # ######################################### AccII AciI AfaI AluI AlwI AlwNI AspHI AvaI AvaII BalI BanI BanII BbsI BbvI BcnI BlpI BmyI BpmI Bpu1102I BpuAI BsaI BsaJI BseRI BsiHKAI BsiYI BslI BsmAI BsmFI BsoBI Bsp1286I BspMI BsrBI BsrDI BsrFI BsrI BssKI BstF5I BstNI BstUI BstXI BstYI Cac8I CelII CfoI Cfr10I Cfr13I DdeI DpnII DraII DsaI EaeI EcoO109I EcoRII FauI Fnu4HI FokI HaeIII HapII HgaI HhaI HinP1I HinfI HpaII HphI ItaI MaeII MaeIII MboI MboII MflI MluNI MnlI MscI MslI MspA1I MspI MvaI MvnI MwoI NciI NdeII NlaIII NlaIV PleI RsaI Sau3AI Sau96I ScrFI SfaNI SmaI TaqI TfiI TseI Tsp45I TspRI TthHB8I XhoII XmaCI XmaI