################################## # NO CUT ENZYMES ( 67 enzymes) # ################################## Acc65I AclI AgeI Aor51HI AscI AseI AsnI Asp718I AviII AvrII BamHI BbrPI BclI BglII BlnI BsaBI BsiEI BspDI BssHII Bst1107I BstBI BstEII BstPI BstZ17I ClaI CpoI EagI EclXI Eco47III Eco52I EcoO65I EcoRV EcoT22I FbaI FspI HindIII HpaI KpnI KspI MamI MfeI MluI MunI NheI NotI NruI NsiI NspV PacI PaeR7I PinAI PmaCI PmeI PmlI PshBI Psp1406I PvuI RsrII SacII SfuI SgrAI SnaBI SpeI SspI SwaI XbaI XhoI ################################### # ONE CUT ENZYMES ( 42 enzymes) # ################################### AatII 2608 GACGTC GACGT^C AflII 904 CTTAAG C^TTAAG Alw44I 3610 GTGCAC G^TGCAC ApaLI 3610 GTGCAC G^TGCAC BalI 1610 TGGCCA TGG^CCA BbeI 1214 GGCGCC GGCGC^C BbsI 3859 GTCTTC GAAGAC(2/6) BbvCI 493 GCTGAGG CCTCAGC(-5/-2) BcgI 2936 GCACTCGGCTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10) BciVI 2574 GGATAC GTATCC(6/5) BfrI 904 CTTAAG C^TTAAG BpuAI 3859 GTCTTC GAAGAC(2/6) BsaAI 3714 CACGTA [CT]AC^GT[GA] BsiWI 3716 CGTACG C^GTACG BsmI 489 GAATGC GAATGC(1/-1) Bsp1407I 116 TGTACA T^GTACA BspLU11I 802 ACATGT A^CATGT BsrGI 116 TGTACA T^GTACA BssSI 2816 CTCGTG CACGAG(-5/-1) DraI 1104 TTTAAA TTT^AAA DraIII 439 CACAAGGTG CAC[ACGT][ACGT][ACGT]^GTG DrdI 3694 GACGAGGGTGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC EcoRI 1917 GAATTC G^AATTC FseI 2389 GGCCGGCC GGCCGG^CC HaeII 1214 GGCGCC [GA]GCGC^[CT] KasI 1214 GGCGCC G^GCGCC MluNI 1610 TGGCCA TGG^CCA MscI 1610 TGGCCA TGG^CCA NaeI 2390 GCCGGC GCC^GGC NarI 1214 GGCGCC GG^CGCC NdeI 1772 CATATG CA^TATG NgoMIV 2390 GCCGGC G^CCGGC PshAI 799 GACACATGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC SapI 273 GAAGAGC GCTCTTC(1/4) SbfI 1803 CCTGCAGG CCTGCA^GG ScaI 224 AGTACT AGT^ACT SexAI 344 ACCTGGT A^CC[AT]GGT SfiI 2384 GGCCAGGCCGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC SfoI 1214 GGCGCC GGC^GCC Sse8387I 1803 CCTGCAGG CCTGCA^GG SspBI 116 TGTACA T^GTACA StuI 1658 AGGCCT AGG^CCT ################################### # TWO CUT ENZYMES ( 34 enzymes) # ################################### AccIII 3032 TCCGGA T^CCGGA 3269 TCCGGA AcsI 1917 GAATTC [GA]^AATT[CT] 3448 AAATTC AcyI 1214 GGCGCC G[GA]^CG[CT]C 2608 GACGTC AflIII 802 ACATGT A^C[GA][CT]GT 3713 ACACGT AhdI 2464 GACTACTCGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC 2806 GACTACCAGTC AocI 411 CCTCAGG CC^T[ACGT]AGG 2208 CCTCAGG ApoI 1917 GAATTC [GA]^AATT[CT] 3448 AAATTC Asp700I 555 GAACACTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC 3045 GAAGCGATTC AspEI 2464 GACTACTCGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC 2806 GACTACCAGTC BglI 2151 GCCTCCAGGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC 2385 GCCAGGCCGGC BlpI 526 GCTCAGC GC^T[ACGT]AGC 2167 GCTCAGC Bpu1102I 526 GCTCAGC GC^T[ACGT]AGC 2167 GCTCAGC BsaHI 1214 GGCGCC G[GA]^CG[CT]C 2608 GACGTC BsaWI 3032 TCCGGA [AT]^CCGG[AT] 3269 TCCGGA BseAI 3032 TCCGGA T^CCGGA 3269 TCCGGA BsmBI 1390 CGTCTC CGTCTC(1/5) 3838 GAGACG BspEI 3032 TCCGGA T^CCGGA 3269 TCCGGA BsrDI 95 GCAATG GCAATG(2/0) 2708 GCAATG BsrFI 1710 GCCGGT [GA]^CCGG[CT] 2390 GCCGGC BstXI 668 CCATCGAGATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 2811 CCAGTCTCGTGG Bsu36I 411 CCTCAGG CC^T[ACGT]AGG 2208 CCTCAGG CelII 526 GCTCAGC GC^T[ACGT]AGC 2167 GCTCAGC Cfr10I 1710 GCCGGT [GA]^CCGG[CT] 2390 GCCGGC Eam1105I 2464 GACTACTCGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC 2806 GACTACCAGTC Eco81I 411 CCTCAGG CC^T[ACGT]AGG 2208 CCTCAGG EcoNI 372 CCTGAAGGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG 2271 CCTCCCCCAGG Hin1I 1214 GGCGCC G[GA]^CG[CT]C 2608 GACGTC MroI 3032 TCCGGA T^CCGGA 3269 TCCGGA SmaI 282 CCCGGG CCC^GGG 3005 CCCGGG SphI 1416 GCATGC GCATG^C 2697 GCATGC Tsp509I 1918 AATT ^AATT 3449 AATT XmaCI 282 CCCGGG C^CCGGG 3005 CCCGGG XmaI 282 CCCGGG C^CCGGG 3005 CCCGGG XmnI 555 GAACACTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC 3045 GAAGCGATTC ######################################### # 3 OR MORE CUT ENZYMES ( 115 enzymes) # ######################################### AccI AccII AciI AfaI AluI AlwI AlwNI ApaI AspHI AspI AvaI AvaII BanI BanII BbvI BcnI BfaI BmyI BpmI BsaI BsaJI BseRI BsgI BsiHKAI BsiYI BslI BsmAI BsmFI BsoBI Bsp1286I BspHI BspMI BsrBI BsrI BssKI BstAPI BstF5I BstNI BstUI BstYI Cac8I CfoI Cfr13I DdeI DpnII DraII DsaI EaeI EarI EcoO109I EcoRII EcoT14I FauI Fnu4HI FokI HaeIII HapII HgaI HhaI HinP1I HincII HindII HinfI HpaII HphI ItaI Ksp632I MaeI MaeII MaeIII MboI MboII MflI MnlI MseI MslI MspA1I MspI MvaI MvnI MwoI NciI NcoI NdeII NlaIII NlaIV NspI PflFI PflMI PleI PpuMI PstI PvuII RcaI RsaI SacI SalI Sau3AI Sau96I ScrFI SfaNI SfcI SmlI StyI TaqI TfiI Tru9I TseI Tsp45I TspRI Tth111I TthHB8I Van91I XcmI XhoII