(Circular) (Six-base) MAPSORT of: pFN21AE5615.seq Check: 2407 from: 1 to: 6418 pFN21AE5615 6418 bp With 182 enzymes: SgfI * December 8, 2014 13:40 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 4228 278 Size: 53 83 186 936 2692 2468 Fragments arranged by size: 2692 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2831 3228 3761 1937 Size: 894 397 533 4594 Fragments arranged by size: 4594 894 533 397 Acc65I G'GTAC_C Cuts at: 2211 3739 2211 Size: 1528 4890 AclI AA'CG_TT Cuts at: 1563 4545 4918 1563 Size: 2982 373 3063 Fragments arranged by size: 3063 2982 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2883 4481 5865 6133 1632 Size: 1251 1598 1384 268 1917 Fragments arranged by size: 1917 1598 1384 1251 268 AflII C'TTAA_G Cuts at: 820 1017 3437 820 Size: 197 2420 3801 Fragments arranged by size: 3801 2420 197 AflIII A'CryG_T Cuts at: 1203 5317 6294 1203 Size: 4114 977 1327 Fragments arranged by size: 4114 1327 977 AgeI A'CCGG_T Cuts at: 5249 5249 Size: 6418 AhdI GACnn_n'nnGTC Cuts at: 5147 5147 Size: 6418 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 6418 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 3723 1184 Size: 2539 3879 AloI GAACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2895 2927 2895 Size: 32 6386 AlwNI CAG_nnn'CTG Cuts at: 4161 5733 4161 Size: 1572 4846 ApaLI G'TGCA_C Cuts at: 2612 4475 5631 2612 Size: 1863 1156 3399 Fragments arranged by size: 3399 1863 1156 ApoI r'AATT_y Cuts at: 1493 2079 2201 2426 2891 3260 3278 3335 3727 Size: 586 122 225 465 369 18 57 392 Cuts at: 3727 3953 4105 6214 1493 Size: 226 152 2109 1697 Fragments arranged by size: 2109 1697 586 465 392 369 226 225 152 122 57 18 AseI AT'TA_AT Cuts at: 160 1032 2694 4972 160 Size: 872 1662 2278 1606 Fragments arranged by size: 2278 1662 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6418 AvaI C'yCGr_G Cuts at: 1870 1943 2304 3743 1870 Size: 73 361 1439 4545 Fragments arranged by size: 4545 1439 361 73 BamHI G'GATC_C Cuts at: 3748 4115 3748 Size: 367 6051 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2211 3739 5194 618 Size: 325 217 627 424 1528 1455 1842 Fragments arranged by size: 1842 1528 1455 627 424 325 217 BanII G_rGCy'C Cuts at: 1391 3737 1391 Size: 2346 4072 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2595 3000 928 Size: 971 696 405 4346 Fragments arranged by size: 4346 971 696 405 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 2915 2949 4609 4643 1903 Size: 34 978 34 1660 34 3678 Fragments arranged by size: 3678 1660 978 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 4310 5520 4310 Size: 1210 5208 BclI T'GATC_A Cuts at: 1548 1548 Size: 6418 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 5029 136 Size: 107 122 71 631 605 3357 1525 Fragments arranged by size: 3357 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 6413 6413 Size: 6418 BlpI GC'TnA_GC Cuts at: 3835 3835 Size: 6418 Bme1580I G_kGCm'C Cuts at: 1790 2616 4479 5635 1790 Size: 826 1863 1156 2573 Fragments arranged by size: 2573 1863 1156 826 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 6418 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 4149 5107 442 Size: 416 53 83 399 24 2732 958 1753 Fragments arranged by size: 2732 1753 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6418 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3590 3590 Size: 6418 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2046 2424 3168 5078 1239 Size: 807 378 744 1910 2579 Fragments arranged by size: 2579 1910 807 744 378 Bpu10I CC'TnA_GC Cuts at: 3631 3631 Size: 6418 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 4543 5408 5706 4543 Size: 865 298 5255 Fragments arranged by size: 5255 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2307 3197 5081 882 Size: 617 808 890 1884 2219 Fragments arranged by size: 2219 1884 890 808 617 BsaAI yAC'GTr Cuts at: 493 1204 4209 493 Size: 711 3005 2702 Fragments arranged by size: 3005 2702 711 BsaBI GATnn'nnATC Cuts at: 2018 6412 2018 Size: 4394 2024 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 4225 4607 5242 275 Size: 53 83 186 936 2692 382 635 1451 Fragments arranged by size: 2692 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 4851 5249 5523 5670 6081 4851 Size: 398 274 147 411 5188 Fragments arranged by size: 5188 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 3539 3569 1178 Size: 30 165 30 2136 30 4027 Fragments arranged by size: 4027 2136 165 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 1186 Size: 6418 BseYI C'CCAG_C Cuts at: 5621 6117 5621 Size: 496 5922 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2521 3274 3678 1163 Size: 573 785 753 404 3903 Fragments arranged by size: 3903 785 753 573 404 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3868 4629 5657 1540 Size: 275 186 1867 761 1028 2301 Fragments arranged by size: 2301 1867 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2616 4479 4564 5635 2616 Size: 1863 85 1071 3399 Fragments arranged by size: 3399 1863 1071 85 BsiWI C'GTAC_G Cuts at: 2844 2844 Size: 6418 BsmI GAATG_Cn' Cuts at: 2808 3929 4022 2808 Size: 1121 93 5204 Fragments arranged by size: 5204 1121 93 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2616 3737 4479 4564 5635 1391 Size: 399 826 1121 742 85 1071 2174 Fragments arranged by size: 2174 1121 1071 826 742 399 85 BspEI T'CCGG_A Cuts at: 6081 6081 Size: 6418 BspHI T'CATG_A Cuts at: 2400 3186 4307 6037 2400 Size: 786 1121 1730 2781 Fragments arranged by size: 2781 1730 1121 786 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 3773 844 Size: 2929 3489 BsrBI CCG'CTC Cuts at: 1394 1823 4305 1394 Size: 429 2482 3507 Fragments arranged by size: 3507 2482 429 BsrDI GCAATG_nn' Cuts at: 65 1248 2977 3707 4913 5087 65 Size: 1183 1729 730 1206 174 1396 Fragments arranged by size: 1729 1396 1206 1183 730 174 BsrFI r'CCGG_y Cuts at: 1700 1810 5062 5249 1700 Size: 110 3252 187 2869 Fragments arranged by size: 3252 2869 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 6418 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6418 BssSI C'ACGA_G Cuts at: 2220 4478 5490 2220 Size: 2258 1012 3148 Fragments arranged by size: 3148 2258 1012 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 6418 BstBI TT'CG_AA Cuts at: 5222 5222 Size: 6418 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 6418 BstXI CCAn_nnnn'nTGG Cuts at: 3119 3119 Size: 6418 BstYI r'GATC_y Cuts at: 1972 3748 4115 4500 4517 5969 6055 6067 6413 Size: 1776 367 385 17 1452 86 12 346 Cuts at: 6413 1972 Size: 1977 Fragments arranged by size: 1977 1776 1452 385 367 346 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2004 2498 513 Size: 552 939 494 4433 Fragments arranged by size: 4433 939 552 494 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5679 Fragments arranged by size: 5679 683 56 BtsI GCAGTG_nn' Cuts at: 2302 3934 4728 4748 2302 Size: 1632 794 20 3972 Fragments arranged by size: 3972 1632 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6418 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 2938 2973 529 Size: 35 2374 35 3974 Fragments arranged by size: 3974 2374 35 35 DraI TTT'AAA Cuts at: 3722 3722 Size: 6418 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 5425 809 Size: 507 4109 1802 Fragments arranged by size: 4109 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2324 2946 3865 Size: 54 1425 50 275 18 494 622 919 Cuts at: 3865 4754 6383 8 Size: 889 1629 43 Fragments arranged by size: 1629 1425 919 889 622 494 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3865 1537 Size: 275 2053 4090 Fragments arranged by size: 4090 2053 275 EarI CTCTTCn'nnn_ Cuts at: 1353 4348 1353 Size: 2995 3423 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 5005 5379 5525 387 Size: 1064 138 3416 374 146 1280 Fragments arranged by size: 3416 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2046 2424 2883 3168 4481 5078 5865 Size: 393 414 378 459 285 1313 597 787 Cuts at: 5865 6133 1239 Size: 268 1524 Fragments arranged by size: 1524 1313 787 597 459 414 393 378 285 268 EcoNI CCTnn'n_nnAGG Cuts at: 3630 3630 Size: 6418 EcoO109I rG'GnC_Cy Cuts at: 3582 3582 Size: 6418 EcoRI G'AATT_C Cuts at: 2079 3260 3278 3727 4105 2079 Size: 1181 18 449 378 4392 Fragments arranged by size: 4392 1181 449 378 18 EcoRV GAT'ATC Cuts at: 2362 2605 2362 Size: 243 6175 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 3544 3544 Size: 6418 FspI TGC'GCA Cuts at: 4924 4924 Size: 6418 HaeII r_GCGC'y Cuts at: 1678 5565 1678 Size: 3887 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 3159 3191 5059 5091 5133 Size: 32 135 32 1587 32 1868 32 42 Cuts at: 5133 5165 1373 Size: 32 2626 Fragments arranged by size: 2626 1868 1587 135 42 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2755 2832 3505 3762 4008 669 Size: 988 281 817 77 673 257 246 3079 Fragments arranged by size: 3079 988 817 673 281 257 246 77 HindIII A'AGCT_T Cuts at: 748 3514 748 Size: 2766 3652 HpaI GTT'AAC Cuts at: 2755 4008 2755 Size: 1253 5165 KpnI G_GTAC'C Cuts at: 2215 3743 2215 Size: 1528 4890 MfeI C'AATT_G Cuts at: 4017 4017 Size: 6418 MluI A'CGCG_T Cuts at: 6294 6294 Size: 6418 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2337 5532 5716 1305 Size: 1032 3195 184 2007 Fragments arranged by size: 3195 2007 1032 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2326 2948 10 Size: 54 1425 343 494 622 3480 Fragments arranged by size: 3480 1425 622 494 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 4376 4735 4894 518 Size: 648 3210 359 159 2042 Fragments arranged by size: 3210 2042 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2804 3834 4511 5659 5904 1606 Size: 1198 1030 677 1148 245 2120 Fragments arranged by size: 2120 1198 1148 1030 677 245 NcoI C'CATG_G Cuts at: 513 1065 2004 513 Size: 552 939 4927 Fragments arranged by size: 4927 939 552 NdeI CA'TA_TG Cuts at: 387 3679 387 Size: 3292 3126 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6418 NotI GC'GGCC_GC Cuts at: 3865 3865 Size: 6418 NspI r_CATG'y Cuts at: 1132 1229 2495 2930 3776 5321 1132 Size: 97 1266 435 846 1545 2229 Fragments arranged by size: 2229 1545 1266 846 435 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 6418 PciI A'CATG_T Cuts at: 5317 5317 Size: 6418 PflMI CCAn_nnn'nTGG Cuts at: 1108 2107 3680 1108 Size: 999 1573 3846 Fragments arranged by size: 3846 1573 999 PfoI T'CCnGG_A Cuts at: 2761 2761 Size: 6418 PmeI GTTT'AAAC Cuts at: 3722 3722 Size: 6418 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4487 4519 6000 6032 4487 Size: 32 1481 32 4873 Fragments arranged by size: 4873 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 3582 3582 Size: 6418 PshAI GACnn'nnGTC Cuts at: 1613 3162 1613 Size: 1549 4869 PsiI TTA'TAA Cuts at: 3988 3988 Size: 6418 PstI C_TGCA'G Cuts at: 830 2488 3770 830 Size: 1658 1282 3478 Fragments arranged by size: 3478 1658 1282 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6418 SalI G'TCGA_C Cuts at: 1936 2830 3760 1936 Size: 894 930 4594 Fragments arranged by size: 4594 930 894 SbfI CC_TGCA'GG Cuts at: 3770 3770 Size: 6418 ScaI AGT'ACT Cuts at: 4666 6378 4666 Size: 1712 4706 SfcI C'TryA_G Cuts at: 826 1045 2241 2484 3577 3766 4901 5582 5773 Size: 219 1196 243 1093 189 1135 681 191 Cuts at: 5773 826 Size: 1471 Fragments arranged by size: 1471 1196 1135 1093 681 243 219 191 189 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6418 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6418 SmaI CCC'GGG Cuts at: 1872 3745 1872 Size: 1873 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2304 3437 4522 5423 5685 820 Size: 197 926 361 1133 1085 901 262 1553 Fragments arranged by size: 1553 1133 1085 926 901 361 262 197 SnaBI TAC'GTA Cuts at: 493 4209 493 Size: 3716 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 6418 SphI G_CATG'C Cuts at: 1132 3776 1132 Size: 2644 3774 SspI AAT'ATT Cuts at: 5 52 3003 4342 5 Size: 47 2951 1339 2081 Fragments arranged by size: 2951 2081 1339 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 6418 StyI C'CwwG_G Cuts at: 513 1065 2004 2327 2704 3562 3857 513 Size: 552 939 323 377 858 295 3074 Fragments arranged by size: 3074 939 858 552 377 323 295 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2615 3414 4445 4462 4615 4800 5245 Size: 1255 1035 799 1031 17 153 185 445 Cuts at: 5245 325 Size: 1498 Fragments arranged by size: 1498 1255 1035 1031 799 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 4664 6376 96 Size: 274 80 33 51 1443 2687 1712 138 Fragments arranged by size: 2687 1712 1443 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 3539 3571 6378 6410 3539 Size: 32 2807 32 3547 Fragments arranged by size: 3547 2807 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 6418 XbaI T'CTAG_A Cuts at: 3754 3754 Size: 6418 XhoI C'TCGA_G Cuts at: 1943 2304 1943 Size: 361 6057 XmaI C'CCGG_G Cuts at: 1870 3743 1870 Size: 1873 4545 XmnI GAAnn'nnTTC Cuts at: 2592 4547 2592 Size: 1955 4463 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 4226 276 Size: 53 83 186 936 2692 2468 Fragments arranged by size: 2692 2468 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AloI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsiWI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoNI EcoO109I EcoRI EcoRV FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PstI PvuI SalI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI ApaI AscI AvrII BaeI BbeI BbvCI BfrBI BsmBI BstZ17I Bsu36I DraIII EcoICRI FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PmlI PspOMI PspXI PsrI PvuII RsrII SacI SacII SanDI SapI SexAI SfiI SfoI SrfI SwaI XcmI