Restriction map for
pFN21AB5994
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# NO CUT ENZYMES ( 141 enzymes) #
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AatII Acc65I AccI AccII AccIII
AcyI AflII AgeI AhdI AlwNI
Aor51HI ApaI AscI AseI AsnI
Asp700I Asp718I AspEI AvaI AviII
BalI BamHI BanI BanII BbeI
BbrPI BcgI BciVI BclI BfrI
BglI BlpI Bpu1102I BsaAI BsaBI
BsaHI BsaWI BseAI BsiEI BsiWI
BsmBI BsmFI BsoBI Bsp1407I BspDI
BspEI BsrBI BsrFI BsrGI BssHII
BssSI Bst1107I BstAPI BstBI BstUI
BstZ17I CelII Cfr10I ClaI CpoI
DraII DraIII DrdI EaeI EagI
Eam1105I EclXI Eco47III Eco52I EcoNI
EcoO109I EcoRI EcoRV EcoT22I FauI
FbaI FseI FspI HaeII HaeIII
HgaI Hin1I HindIII HpaI KasI
KpnI KspI MamI MfeI MluI
MluNI MroI MscI MunI MvnI
NaeI NarI NgoMIV NheI NlaIV
NotI NruI NsiI NspV PacI
PaeR7I PflMI PinAI PmaCI PmeI
PmlI PpuMI PshAI PshBI PvuI
RsrII SacI SacII SalI SbfI
ScaI SexAI SfiI SfoI SfuI
SgrAI SmaI SnaBI SpeI SphI
Sse8387I SspBI SspI StuI SwaI
Van91I XcmI XhoI XmaCI XmaI
XmnI
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# ONE CUT ENZYMES ( 47 enzymes) #
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AclI 1587 AACGTT AA^CGTT
AflIII 226 ACATGT A^C[GA][CT]GT
Alw44I 441 GTGCAC G^TGCAC
AlwI 1165 GATCC GGATC(4/5)
AocI 1817 CCTTAGG CC^T[ACGT]AGG
ApaLI 441 GTGCAC G^TGCAC
AspHI 441 GTGCAC G[AT]GC[AT]^C
AspI 1483 GACAGAGTC GAC[ACGT]^[ACGT][ACGT]GTC
AvrII 1359 CCTAGG C^CTAGG
BbvCI 1030 GCTGAGG CCTCAGC(-5/-2)
BcnI 525 CCCGG CC^[GC]GG
BlnI 1359 CCTAGG C^CTAGG
BmyI 441 GTGCAC G[AGT]GC[ACT]^C
BpmI 885 CTCCAG CTGGAG(16/14)
BseRI 1780 GAGGAG GAGGAG(10/8)
BsiHKAI 441 GTGCAC G[AT]GC[AT]^C
BsiYI 408 CCCCCGATTGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
BslI 408 CCCCCGATTGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
Bsp1286I 441 GTGCAC G[AGT]GC[ACT]^C
BspLU11I 226 ACATGT A^CATGT
BspMI 1768 ACCTGC ACCTGC(4/8)
BsrDI 939 CATTGC GCAATG(2/0)
BstEII 9 GGTAACC G^GT[ACGT]ACC
BstPI 9 GGTAACC G^GT[ACGT]ACC
BstXI 681 CCAAAAATTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
Bsu36I 1817 CCTTAGG CC^T[ACGT]AGG
CfoI 1288 GCGC GCG^C
Eco81I 1817 CCTTAGG CC^T[ACGT]AGG
EcoO65I 9 GGTAACC G^GT[ACGT]ACC
HapII 526 CCGG C^CGG
HhaI 1288 GCGC GCG^C
HinP1I 1288 GCGC G^CGC
HincII 1326 GTCAAC GT[CT]^[GA]AC
HindII 1326 GTCAAC GT[CT]^[GA]AC
HpaII 526 CCGG C^CGG
MspI 526 CCGG C^CGG
NciI 525 CCCGG CC^[GC]GG
NcoI 810 CCATGG C^CATGG
NdeI 1127 CATATG CA^TATG
PflFI 1483 GACAGAGTC GAC[ACGT]^[ACGT][ACGT]GTC
Psp1406I 1587 AACGTT AA^CGTT
PstI 1160 CTGCAG CTGCA^G
PvuII 245 CAGCTG CAG^CTG
SapI 1216 GAAGAGC GCTCTTC(1/4)
SfcI 1160 CTGCAG C^T[GA][CT]AG
Tth111I 1483 GACAGAGTC GAC[ACGT]^[ACGT][ACGT]GTC
XbaI 1140 TCTAGA T^CTAGA
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# TWO CUT ENZYMES ( 21 enzymes) #
###################################
AciI 1157 CCGC CCGC(-3/-1)
844 GCGG
AfaI 1272 GTAC GT^AC
1315 GTAC
AvaII 846 GGACC G^G[AT]CC
1437 GGTCC
BfaI 1141 CTAG C^TAG
1360 CTAG
BglII 780 AGATCT A^GATCT
816 AGATCT
BspHI 453 TCATGA T^CATGA
1170 TCATGA
BstNI 53 CCAGG CC^[AT]GG
1050 CCTGG
Cfr13I 846 GGACC G^G[ACGT]CC
1437 GGTCC
DraI 103 TTTAAA TTT^AAA
1201 TTTAAA
DsaI 810 CCATGG C^C[GA][CT]GG
1433 CCGTGG
EcoRII 53 CCAGG ^CC[AT]GG
1050 CCTGG
MaeI 1141 CTAG C^TAG
1360 CTAG
MaeII 1562 ACGT A^CGT
1588 ACGT
MspA1I 245 CAGCTG C[AC]G^C[GT]G
1157 CCGCTG
MvaI 53 CCAGG CC^[AT]GG
1050 CCTGG
NspI 226 ACATGT [GA]CATG^[CT]
1055 GCATGT
RcaI 453 TCATGA T^CATGA
1170 TCATGA
RsaI 1272 GTAC GT^AC
1315 GTAC
Sau96I 846 GGACC G^G[ACGT]CC
1437 GGTCC
SmlI 664 CTCAAG C^T[CT][GA]AG
1350 CTCAAG
TfiI 877 GATTC G^A[AT]TC
1507 GAATC
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# 3 OR MORE CUT ENZYMES ( 49 enzymes) #
#########################################
AcsI AluI ApoI BbsI BbvI
BpuAI BsaI BsaJI BsgI BsmAI
BsmI BsrI BssKI BstF5I BstYI
Cac8I DdeI DpnII EarI EcoT14I
Fnu4HI FokI HinfI HphI ItaI
Ksp632I MaeIII MboI MboII MflI
MnlI MseI MslI MwoI NdeII
NlaIII PleI Sau3AI ScrFI SfaNI
StyI TaqI Tru9I TseI Tsp45I
Tsp509I TspRI TthHB8I XhoII