(Circular) (Six-base) MAPSORT of: pFN21AB5994.seq Check: 9052 from: 1 to: 6532 pFN21AB5994 6532 bp With 182 enzymes: SgfI * December 8, 2014 13:55 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 4342 278 Size: 53 83 186 936 2806 2468 Fragments arranged by size: 2806 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3875 1937 Size: 1938 4594 Acc65I G'GTAC_C Cuts at: 3853 3853 Size: 6532 AclI AA'CG_TT Cuts at: 1563 3593 4659 5032 1563 Size: 2030 1066 373 3063 Fragments arranged by size: 3063 2030 1066 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2153 2921 3420 4595 5979 6247 1632 Size: 521 768 499 1175 1384 268 1917 Fragments arranged by size: 1917 1384 1175 768 521 499 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 6335 AflIII A'CryG_T Cuts at: 1203 2231 5431 6408 1203 Size: 1028 3200 977 1327 Fragments arranged by size: 3200 1327 1028 977 AgeI A'CCGG_T Cuts at: 5363 5363 Size: 6532 AhdI GACnn_n'nnGTC Cuts at: 5261 5261 Size: 6532 AleI CACnn'nnGTG Cuts at: 1166 2088 1166 Size: 922 5610 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 3631 1184 Size: 2447 4085 AlwNI CAG_nnn'CTG Cuts at: 4275 5847 4275 Size: 1572 4960 ApaLI G'TGCA_C Cuts at: 2446 4589 5745 2446 Size: 2143 1156 3233 Fragments arranged by size: 3233 2143 1156 ApoI r'AATT_y Cuts at: 1493 2128 2219 2690 3203 3232 3281 3841 4067 Size: 635 91 471 513 29 49 560 226 Cuts at: 4067 4219 6328 1493 Size: 152 2109 1697 Fragments arranged by size: 2109 1697 635 560 513 471 226 152 91 49 29 AseI AT'TA_AT Cuts at: 160 1032 5086 160 Size: 872 4054 1606 Fragments arranged by size: 4054 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6532 AvaI C'yCGr_G Cuts at: 1870 1943 3857 1870 Size: 73 1914 4545 Fragments arranged by size: 4545 1914 73 AvrII C'CTAG_G Cuts at: 3364 3364 Size: 6532 BamHI G'GATC_C Cuts at: 3862 4229 3862 Size: 367 6165 BanI G'GyrC_C Cuts at: 618 943 1160 1787 3853 5308 618 Size: 325 217 627 2066 1455 1842 Fragments arranged by size: 2066 1842 1455 627 325 217 BanII G_rGCy'C Cuts at: 1391 3851 1391 Size: 2460 4072 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2515 3043 3432 928 Size: 971 616 528 389 4028 Fragments arranged by size: 4028 971 616 528 389 BbvCI CC'TCA_GC Cuts at: 3036 3036 Size: 6532 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 4723 4757 1903 Size: 34 2786 34 3678 Fragments arranged by size: 3678 2786 34 34 BciVI GTATCCnnnnn_n' Cuts at: 4424 5634 4424 Size: 1210 5322 BclI T'GATC_A Cuts at: 1548 1548 Size: 6532 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 5143 136 Size: 107 122 71 631 605 3471 1525 Fragments arranged by size: 3471 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 2785 2821 6527 2785 Size: 36 3706 2790 Fragments arranged by size: 3706 2790 36 BlpI GC'TnA_GC Cuts at: 3949 3949 Size: 6532 Bme1580I G_kGCm'C Cuts at: 1790 2450 4593 5749 1790 Size: 660 2143 1156 2573 Fragments arranged by size: 2573 2143 1156 660 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 6532 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 4263 5221 442 Size: 416 53 83 399 24 2846 958 1753 Fragments arranged by size: 2846 1753 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6532 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2875 5192 1239 Size: 1636 2317 2579 Fragments arranged by size: 2579 2317 1636 Bpu10I CC'TnA_GC Cuts at: 2732 3036 2732 Size: 304 6228 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2654 3340 4657 5522 5820 2654 Size: 686 1317 865 298 3366 Fragments arranged by size: 3366 1317 865 686 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2419 3358 3764 5195 882 Size: 617 920 939 406 1431 2219 Fragments arranged by size: 2219 1431 939 920 617 406 BsaAI yAC'GTr Cuts at: 493 1204 4323 493 Size: 711 3119 2702 Fragments arranged by size: 3119 2702 711 BsaBI GATnn'nnATC Cuts at: 6526 6526 Size: 6532 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 4339 4721 5356 275 Size: 53 83 186 936 2806 382 635 1451 Fragments arranged by size: 2806 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 4965 5363 5637 5784 6195 4965 Size: 398 274 147 411 5302 Fragments arranged by size: 5302 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 6307 Fragments arranged by size: 6307 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 3800 1186 Size: 2614 3918 BseYI C'CCAG_C Cuts at: 5735 6231 5735 Size: 496 6036 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 3441 3490 3642 1163 Size: 573 1705 49 152 4053 Fragments arranged by size: 4053 1705 573 152 49 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3982 4743 5771 1540 Size: 275 186 1981 761 1028 2301 Fragments arranged by size: 2301 1981 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2450 4593 4678 5749 2450 Size: 2143 85 1071 3233 Fragments arranged by size: 3233 2143 1071 85 BsmI GAATG_Cn' Cuts at: 2381 2813 2912 3185 3295 3417 4043 4136 2381 Size: 432 99 273 110 122 626 93 4777 Fragments arranged by size: 4777 626 432 273 122 110 99 93 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2450 3851 4593 4678 5749 1391 Size: 399 660 1401 742 85 1071 2174 Fragments arranged by size: 2174 1401 1071 742 660 399 85 BspEI T'CCGG_A Cuts at: 6195 6195 Size: 6532 BspHI T'CATG_A Cuts at: 2458 3175 4421 6151 2458 Size: 717 1246 1730 2839 Fragments arranged by size: 2839 1730 1246 717 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 3782 3887 844 Size: 2938 105 3489 Fragments arranged by size: 3489 2938 105 BsrBI CCG'CTC Cuts at: 1394 1823 4419 1394 Size: 429 2596 3507 Fragments arranged by size: 3507 2596 429 BsrDI GCAATG_nn' Cuts at: 65 1248 2943 5027 5201 65 Size: 1183 1695 2084 174 1396 Fragments arranged by size: 2084 1695 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 5176 5363 1700 Size: 110 3366 187 2869 Fragments arranged by size: 3366 2869 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 6532 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6532 BssSI C'ACGA_G Cuts at: 4592 5604 4592 Size: 1012 5520 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 6532 BstBI TT'CG_AA Cuts at: 5336 5336 Size: 6532 BstEII G'GTnAC_C Cuts at: 1184 2014 1184 Size: 830 5702 BstXI CCAn_nnnn'nTGG Cuts at: 2693 2693 Size: 6532 BstYI r'GATC_y Cuts at: 1972 2785 2821 3169 3862 4229 4614 4631 6083 Size: 813 36 348 693 367 385 17 1452 Cuts at: 6083 6169 6181 6527 1972 Size: 86 12 346 1977 Fragments arranged by size: 1977 1452 813 693 385 367 348 346 86 36 17 12 Bsu36I CC'TnA_GG Cuts at: 3823 3823 Size: 6532 BtgI C'CryG_G Cuts at: 513 1065 2815 3438 513 Size: 552 1750 623 3607 Fragments arranged by size: 3607 1750 623 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5793 Fragments arranged by size: 5793 683 56 BtsI GCAGTG_nn' Cuts at: 2180 2448 2938 4048 4842 4862 2180 Size: 268 490 1110 794 20 3850 Fragments arranged by size: 3850 1110 794 490 268 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6532 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 6497 DraI TTT'AAA Cuts at: 2110 3208 3836 2110 Size: 1098 628 4806 Fragments arranged by size: 4806 1098 628 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 5539 809 Size: 507 4223 1802 Fragments arranged by size: 4223 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 3979 4868 6497 Size: 54 1425 50 275 18 2149 889 1629 Cuts at: 6497 8 Size: 43 Fragments arranged by size: 2149 1629 1425 889 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3979 1537 Size: 275 2167 4090 Fragments arranged by size: 4090 2167 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2319 2463 3216 4462 1353 Size: 966 144 753 1246 3423 Fragments arranged by size: 3423 1246 966 753 144 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2864 5119 5493 5639 387 Size: 1064 138 1275 2255 374 146 1280 Fragments arranged by size: 2255 1280 1275 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2153 2875 2921 3420 4595 5192 5979 Size: 393 521 722 46 499 1175 597 787 Cuts at: 5979 6247 1239 Size: 268 1524 Fragments arranged by size: 1524 1175 787 722 597 521 499 393 268 46 EcoRI G'AATT_C Cuts at: 3841 4219 3841 Size: 378 6154 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2532 3067 2532 Size: 535 5997 FspI TGC'GCA Cuts at: 5038 5038 Size: 6532 HaeII r_GCGC'y Cuts at: 1678 5679 1678 Size: 4001 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2873 2905 3419 3451 5173 Size: 32 135 32 1301 32 514 32 1722 Cuts at: 5173 5205 5247 5279 1373 Size: 32 42 32 2626 Fragments arranged by size: 2626 1722 1301 514 135 42 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3333 3876 4122 669 Size: 988 281 1395 543 246 3079 Fragments arranged by size: 3079 1395 988 543 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 6532 HpaI GTT'AAC Cuts at: 4122 4122 Size: 6532 KpnI G_GTAC'C Cuts at: 3857 3857 Size: 6532 MfeI C'AATT_G Cuts at: 4131 4131 Size: 6532 MluI A'CGCG_T Cuts at: 6408 6408 Size: 6532 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2907 3362 5646 5830 1305 Size: 1602 455 2284 184 2007 Fragments arranged by size: 2284 2007 1602 455 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 4710 Fragments arranged by size: 4710 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2088 2394 2463 4490 4849 5008 518 Size: 648 922 306 69 2027 359 159 2042 Fragments arranged by size: 2042 2027 922 648 359 306 159 69 MspA1I CmG'CkG Cuts at: 1606 2252 3164 3948 4625 5773 6018 1606 Size: 646 912 784 677 1148 245 2120 Fragments arranged by size: 2120 1148 912 784 677 646 245 NcoI C'CATG_G Cuts at: 513 1065 2815 513 Size: 552 1750 4230 Fragments arranged by size: 4230 1750 552 NdeI CA'TA_TG Cuts at: 387 3133 387 Size: 2746 3786 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6532 NotI GC'GGCC_GC Cuts at: 3979 3979 Size: 6532 NspI r_CATG'y Cuts at: 1132 1229 2235 3064 3890 5435 1132 Size: 97 1006 829 826 1545 2229 Fragments arranged by size: 2229 1545 1006 829 826 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 6532 PciI A'CATG_T Cuts at: 2231 5431 2231 Size: 3200 3332 PflMI CCAn_nnn'nTGG Cuts at: 1108 2010 1108 Size: 902 5630 PmeI GTTT'AAAC Cuts at: 3836 3836 Size: 6532 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4601 4633 6114 6146 4601 Size: 32 1481 32 4987 Fragments arranged by size: 4987 1481 32 32 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 6532 PsiI TTA'TAA Cuts at: 4102 4102 Size: 6532 PstI C_TGCA'G Cuts at: 830 3169 3884 830 Size: 2339 715 3478 Fragments arranged by size: 3478 2339 715 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6532 PvuII CAG'CTG Cuts at: 2252 2252 Size: 6532 SalI G'TCGA_C Cuts at: 1936 3874 1936 Size: 1938 4594 SapI GCTCTTCn'nnn_ Cuts at: 3216 3216 Size: 6532 SbfI CC_TGCA'GG Cuts at: 3884 3884 Size: 6532 ScaI AGT'ACT Cuts at: 4780 6492 4780 Size: 1712 4820 SfcI C'TryA_G Cuts at: 826 1045 3165 3880 5015 5696 5887 826 Size: 219 2120 715 1135 681 191 1471 Fragments arranged by size: 2120 1471 1135 715 681 219 191 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6532 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6532 SmaI CCC'GGG Cuts at: 1872 3859 1872 Size: 1987 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2669 3355 4636 5537 5799 820 Size: 197 926 726 686 1281 901 262 1553 Fragments arranged by size: 1553 1281 926 901 726 686 262 197 SnaBI TAC'GTA Cuts at: 493 4323 493 Size: 3830 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 6532 SphI G_CATG'C Cuts at: 1132 3890 1132 Size: 2758 3774 SspI AAT'ATT Cuts at: 5 52 4456 5 Size: 47 4404 2081 Fragments arranged by size: 4404 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 6532 StyI C'CwwG_G Cuts at: 513 1065 2139 2815 3364 3516 3687 3971 513 Size: 552 1074 676 549 152 171 284 3074 Fragments arranged by size: 3074 1074 676 552 549 284 171 152 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 4559 4576 4729 4914 5359 325 Size: 1255 2979 17 153 185 445 1498 Fragments arranged by size: 2979 1498 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 3276 4778 6490 Size: 274 80 33 51 1443 1299 1502 1712 Cuts at: 6490 96 Size: 138 Fragments arranged by size: 1712 1502 1443 1299 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 6492 6524 6492 Size: 32 6500 Tth111I GACn'n_nGTC Cuts at: 1316 3491 1316 Size: 2175 4357 XbaI T'CTAG_A Cuts at: 3145 3868 3145 Size: 723 5809 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 6532 XmaI C'CCGG_G Cuts at: 1870 3857 1870 Size: 1987 4545 XmnI GAAnn'nnTTC Cuts at: 4661 4661 Size: 6532 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 4340 276 Size: 53 83 186 936 2806 2468 Fragments arranged by size: 2806 2468 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI AvrII BamHI BanI BanII BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PmeI PpiI PshAI PsiI PstI PvuI PvuII SalI SapI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI ApaI AscI BaeI BbeI BfrBI BplI BsiWI BsmBI BstZ17I DraIII EcoICRI EcoNI EcoO109I EcoRV FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PfoI PmlI PpuMI PspOMI PspXI PsrI RsrII SacI SacII SanDI SexAI SfiI SfoI SrfI SwaI XcmI