Restriction map for
pFN21AB5994
(Linear) (Six-base) MAPSORT of: KIBB5994.seq Check: 3165 from: 1 to: 1827
KIBB5994.orf
With 182 enzymes: SgfI *
July 10, 2012 13:28 ..
AclI AA'CG_TT
Cuts at: 0 1588 1827
Size: 1588 239
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 148 916 1415 1827
Size: 148 768 499 412
Fragments arranged by size:
768 499 412 148
AflIII A'CryG_T
Cuts at: 0 226 1827
Size: 226 1601
AleI CACnn'nnGTG
Cuts at: 0 83 1827
Size: 83 1744
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 1626 1827
Size: 1626 201
ApaLI G'TGCA_C
Cuts at: 0 441 1827
Size: 441 1386
ApoI r'AATT_y
Cuts at: 0 123 214 685 1198 1227 1276 1827
Size: 123 91 471 513 29 49 551
Fragments arranged by size:
551 513 471 123 91 49 29
AvrII C'CTAG_G
Cuts at: 0 1359 1827
Size: 1359 468
BbsI GAAGACnn'nnnn_
Cuts at: 0 510 1038 1427 1827
Size: 510 528 389 400
Fragments arranged by size:
528 510 400 389
BbvCI CC'TCA_GC
Cuts at: 0 1031 1827
Size: 1031 796
BglII A'GATC_T
Cuts at: 0 780 816 1827
Size: 780 36 1011
Fragments arranged by size:
1011 780 36
Bme1580I G_kGCm'C
Cuts at: 0 445 1827
Size: 445 1382
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 870 1827
Size: 870 957
Bpu10I CC'TnA_GC
Cuts at: 0 727 1031 1827
Size: 727 304 796
Fragments arranged by size:
796 727 304
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 649 1335 1827
Size: 649 686 492
Fragments arranged by size:
686 649 492
BsaI GGTCTCn'nnnn_
Cuts at: 0 414 1353 1759 1827
Size: 414 939 406 68
Fragments arranged by size:
939 414 406 68
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 1795 1827
Size: 1795 32
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1436 1485 1637 1827
Size: 1436 49 152 190
Fragments arranged by size:
1436 190 152 49
BsiHKAI G_wGCw'C
Cuts at: 0 445 1827
Size: 445 1382
BsmI GAATG_Cn'
Cuts at: 0 376 808 907 1180 1290 1412 1827
Size: 376 432 99 273 110 122 415
Fragments arranged by size:
432 415 376 273 122 110 99
Bsp1286I G_dGCh'C
Cuts at: 0 445 1827
Size: 445 1382
BspHI T'CATG_A
Cuts at: 0 453 1170 1827
Size: 453 717 657
Fragments arranged by size:
717 657 453
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 1777 1827
Size: 1777 50
BsrDI GCAATG_nn'
Cuts at: 0 938 1827
Size: 938 889
BstEII G'GTnAC_C
Cuts at: 0 9 1827
Size: 9 1818
BstXI CCAn_nnnn'nTGG
Cuts at: 0 688 1827
Size: 688 1139
BstYI r'GATC_y
Cuts at: 0 780 816 1164 1827
Size: 780 36 348 663
Fragments arranged by size:
780 663 348 36
Bsu36I CC'TnA_GG
Cuts at: 0 1818 1827
Size: 1818 9
BtgI C'CryG_G
Cuts at: 0 810 1433 1827
Size: 810 623 394
Fragments arranged by size:
810 623 394
BtsI GCAGTG_nn'
Cuts at: 0 175 443 933 1827
Size: 175 268 490 894
Fragments arranged by size:
894 490 268 175
DraI TTT'AAA
Cuts at: 0 105 1203 1827
Size: 105 1098 624
Fragments arranged by size:
1098 624 105
EarI CTCTTCn'nnn_
Cuts at: 0 314 458 1211 1827
Size: 314 144 753 616
Fragments arranged by size:
753 616 314 144
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 859 1827
Size: 859 968
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 148 870 916 1415 1827
Size: 148 722 46 499 412
Fragments arranged by size:
722 499 412 148 46
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 527 1062 1827
Size: 527 535 765
Fragments arranged by size:
765 535 527
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 868 900 1414 1446 1827
Size: 868 32 514 32 381
Fragments arranged by size:
868 514 381 32 32
HincII GTy'rAC
Cuts at: 0 1328 1827
Size: 1328 499
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 902 1357 1827
Size: 902 455 470
Fragments arranged by size:
902 470 455
MslI CAynn'nnrTG
Cuts at: 0 83 389 458 1827
Size: 83 306 69 1369
Fragments arranged by size:
1369 306 83 69
MspA1I CmG'CkG
Cuts at: 0 247 1159 1827
Size: 247 912 668
Fragments arranged by size:
912 668 247
NcoI C'CATG_G
Cuts at: 0 810 1827
Size: 810 1017
NdeI CA'TA_TG
Cuts at: 0 1128 1827
Size: 1128 699
NspI r_CATG'y
Cuts at: 0 230 1059 1827
Size: 230 829 768
Fragments arranged by size:
829 768 230
PciI A'CATG_T
Cuts at: 0 226 1827
Size: 226 1601
PstI C_TGCA'G
Cuts at: 0 1164 1827
Size: 1164 663
PvuII CAG'CTG
Cuts at: 0 247 1827
Size: 247 1580
SapI GCTCTTCn'nnn_
Cuts at: 0 1211 1827
Size: 1211 616
SfcI C'TryA_G
Cuts at: 0 1160 1827
Size: 1160 667
SmlI C'TyrA_G
Cuts at: 0 664 1350 1827
Size: 664 686 477
Fragments arranged by size:
686 664 477
StyI C'CwwG_G
Cuts at: 0 134 810 1359 1511 1682 1827
Size: 134 676 549 152 171 145
Fragments arranged by size:
676 549 171 152 145 134
TatI w'GTAC_w
Cuts at: 0 1271 1827
Size: 1271 556
Tth111I GACn'n_nGTC
Cuts at: 0 1486 1827
Size: 1486 341
XbaI T'CTAG_A
Cuts at: 0 1140 1827
Size: 1140 687
Enzymes that do cut:
AclI AcuI AflIII AleI AlfI ApaLI ApoI AvrII
BbsI BbvCI BglII Bme1580I BpmI Bpu10I BpuEI BsaI
BseRI BsgI BsiHKAI BsmI Bsp1286I BspHI BspMI BsrDI
BstEII BstXI BstYI Bsu36I BtgI BtsI DraI EarI
EciI Eco57MI FalI Hin4I HincII MmeI MslI MspA1I
NcoI NdeI NspI PciI PstI PvuII SapI SfcI
SmlI StyI TatI Tth111I XbaI
Enzymes that do not cut:
AarI AatII AccI Acc65I AfeI AflII AgeI AhdI
AloI AlwNI ApaI AscI AseI AsiSI AvaI BaeI
BamHI BanI BanII BbeI BcgI BciVI BclI BfrBI
BglI BlpI BmgBI BmrI BmtI BplI BsaAI BsaBI
BsaHI BsaWI BsaXI BseYI BsiEI BsiWI BsmBI BspEI
BsrBI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstZ17I
BtgZI ClaI CspCI DraIII DrdI EaeI EagI EcoICRI
EcoNI EcoO109I EcoRI EcoRV FseI FspI FspAI HaeII
HindIII HpaI KasI KpnI MfeI MluI MscI NaeI
NarI NgoMIV NheI NotI NruI NsiI PacI PasI
PflMI PfoI PmeI PmlI PpiI PpuMI PshAI PsiI
PspOMI PspXI PsrI PvuI RsrII SacI SacII SalI
SanDI SbfI ScaI SexAI SfiI SfoI SgfI SgrAI
SmaI SnaBI SpeI SphI SrfI SspI StuI SwaI
TaqII TstI XcmI XhoI XmaI XmnI ZraI